著者
早野 順一郎 山田 眞己 藤浪 隆夫 横山 清子 渡辺 與作 高田 和之
出版者
The Biophysical Society of Japan General Incorporated Association
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.28, no.4, pp.198-202, 1988
被引用文献数
41 11

Method for spectral analysis of heart rate variability (HRV), and physiological characteristics of their spectral components are discussed. Respiratory component has a liner relationship with cardiac parasympathetic activity. Other component ranging around 0.1Hz is related to Mayer wave of arterial blood pressure. They provide a quantitative index of autonomic nervous function.
著者
竹口 紀晃
出版者
The Biophysical Society of Japan General Incorporated Association
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.23, no.6, pp.283-290, 1983

Fluxes of ions such as H<SUP>+</SUP>, Cl<SUP>-</SUP>, K<SUP>+</SUP>, Na<SUP>+</SUP>, and HCO<SUB>3</SUB><SUP>-</SUP> in the parietal cell are summarized. Gastric vesicles containing membrane (H<SUP>+</SUP>+K<SUP>+</SUP>)-ATPase are isolated from the parietal cell and can accumulate H<SUP>+</SUP> in exchange for K<SUP>+</SUP> (H<SUP>+</SUP>/K<SUP>+</SUP> antiport, nonelectrogenic) on the addition of MgATP. The intravesicular pH falls as low as 1-3, dependending on the methods used for estimation. The biochemical properties of the ATPase are summarized. Onset of acid secretion changes the morphology of the parietal cell at the secretory surface. This is explained by the increase in KCl conductance of gastric vesicle membrane. The increase is due to the opening of the anion channel caused by S-S cross-linkings.
著者
小松 俊朗 北島 博己 吉原 透
出版者
The Biophysical Society of Japan General Incorporated Association
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.20, no.5, pp.297-305, 1980

Recent studies of quantum chemical calculations on visual pigments are summerized.<BR>It is generally accepted that the red-shift and the wide distribution of λ<SUB>max'</SUB>s (wavelengths of absorption maximum) of visual pigments can be explained by the protonated Schiff-base binding of retinal with opsin and by the electrostatic interaction between retinal and a counter-ion on opsin. Experimental and theoretical evidences for this primary linkage and interaction are first presented and discussed.<BR>Secondly, recent studies of the secondary in teractions between retinal and opsin are summarized. At present, the following three types of the secondary interactions are mainly investigated: (1) the interaction of retinal through itsβ-ionone ring; (2) the interaction through its 9-methyl group; (3) the interaction with the local electric field due to the microenvironment of chromophore.<BR>Thirdly, as for the photochemical conversion of rhodopsin to bathorhodopsin, it is generally considered that retinal isomerizes from 11-cis to all-trans form. Concerning to the mechanism of this isomerization, the potential surfaces of the ground and excited states have recently been examined by several authors. Their models for the intermediates in photobleaching process are reviewed and discussed briefly.
著者
藤田 克昌
出版者
The Biophysical Society of Japan General Incorporated Association
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.50, no.4, pp.174-179, 2010-07-25
被引用文献数
4 1

Recent developments in fluorescence microscopy techniques have broken the diffraction limit and achieved the spatial resolution of sub 100 nm range. Saturated excitation (SAX) microscopy and stimulated emission depletion (STED) microscopy utilize saturable optical phenomena seen in laser excitation and stimulate emission of fluorescence molecules to induce strongly nonlinear optical effects for the resolution improvement. Photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) applied photoswitchable fluorescence probes for precise measurement of positions of the fluorescence probes in a sample in a few tens of nanometer scale. This review introduces the principles and the characteristics of those super resolution microscopy techniques with discussing the imaging formation and the resolution limit in conventional microscopy techniques.<br>
著者
柳田 敏雄
出版者
The Biophysical Society of Japan General Incorporated Association
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.50, no.3, pp.116-117, 2010-05-25
被引用文献数
1 1
著者
大西 武雄
出版者
The Biophysical Society of Japan General Incorporated Association
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.26, no.1, pp.11-19, 1986
被引用文献数
3

Most of the investigations of ultraviolet(UV)-radiation effects on organisms have been made with 254nm from a germicidal lamp. The specific DNA lesion, pyrimidine dimers, caused by UV irradiation have been shown to be toxic, mutagenic and carcinogenic. In contrast, knowledge of the biological effects of far UV(UV-C) radiation may not be relevant to the carcinogenic, mutagenic and lethal effects of UV in sunlight. Recently, since it has been indicated that the DNA lesions caused by mid-UV(280-320nm, UV-B) and nearUV(320-400nm, UV-A) is harmful to a wide variety of organisms, the studies of mid-UV and near-UV effects are considered to be important. The possible DNA damages caused by mid-UV and near-UV are summarized in the present review. In mid-UV range, both the pyrimidine dimers and other photoproducts such as 5-hydroxy-methyl cytosine may be responsible for the observed biological effects and they are repairable by excision repair systems or T4 endonuclease. Several DNA damages of near-UV are indicated to be DNA strand breaks dependent on pol<SUP>+</SUP> and nur<SUP>+</SUP> repair systems, DNA-protein cross-links and a small amount of pyrimidine dimers which are responsible to repair deficint mutants including human patients. In addition, other kinds of photodamages in tRNA carrying cytidine 13 and thiouridine 8 link than DNA damage are indicated to also interact to repair processes.