著者
中村 彰彦 石田 卓也 鮫島 正浩 五十嵐 圭日子
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.57, no.1, pp.59-65, 2015-02-28 (Released:2015-03-05)
参考文献数
7

Structural analysis including hydrogen position is important for estimating the reaction mechanism of hydrolases. We applied neutron and X-ray structural analysis to a mysterious β-1,4 glucanase from a fungi Phanerochaete chrysosporium (PcCel45A). The joint analysis of neutron and X-ray structures showed that PcCel45A utilizing an imidic acid form of asparagine residue as a general base instead of typical acidic residues.