文献一覧: 佐藤光毅 (著者)

1 0 0 0 OA 非鍛錬者における持久性運動負荷および8週間のトレーニングが好中球活性酸素種産生能に及ぼす影響

著者: 佐藤英樹鈴木克彦中路重之菅原和夫戸塚学佐藤光毅
出版者: The Japanese Society for Hygiene
雑誌: 日本衛生学雑誌 (ISSN:00215082)
巻号頁・発行日: vol.53, no.2, pp.431-440, 1998-07-15 (Released:2009-02-17)
参考文献数: 35
被引用文献数: 12 14

We investigated the effects of acute endurance exercise and habitual physical activity for health maintenance on human neutrophil function in 12 untrained men. The acute exercise condition was a continuous exercise for 90 minutes at the intensity of 50% and 55% of maximal oxygen uptake (VO2max) on an ergometer. The training program was 3km jogging three times per week for 8 weeks. The capacity of neutrophils to produce reactive oxygen species (ROS) was detected with lucigenin-dependent chemiluminescence (LgCL) and luminol-dependent chemiluminescence (LmCL) on stimulation with opsonized zymosan (OZ) and phorbol myristate acetate (PMA).As for the acute exercise effects, both LgCL and LmCL responses of neutrophils stimulated using PMA consistently increased after exercise at 50% VO2max, whereas those stimulated with OZ remained unchanged. At 55% VO2max, LgCL responses to both stimulants increase maximally 1h after exercise, and then decreased 3h after exercise, whereas LmCL responses to both stimulants increased continuously after exercise at 55% VO2max. These phenomena observed at 55% VO2max compared to 50% VO2max suggests the improved capacity of producing ROS neutrophils after exercise. The number of neutrophils also increased maximally 1h after exercise, due to the mobilization of band neutrophils (shift to the left), suggesting that functional changes was associated with cell mobilization. The increase in the capacity of neutrophils to produce ROS and marked neutrophilia following the acute endurance exercise suggests that a large quantity of ROS may be produced in vivo.As for the training effects, the LgCL and LmCL responses were maintained in the exercise group as compared to the decreased ones in the control group. The difference between the exercise group and the control group was observed only in LgCL response to OZ. Humoral immune factors (IgG, IgA, IgM, C3, C4) and serum opsonic activity were also unaltered. These phenomena suggest that homeostasis might be kept constant in terms of immunity through regular physical activity.

2020-04-18 14:26:43
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1 0 0 0 OA スポーツ選手における最大運動負荷の血中白血球数と好中球活性酸素産生能に及ぼす影響

著者: 鈴木克彦佐藤英樹遠藤哲長谷川裕子望月充邦中路重之菅原和夫戸塚学佐藤光毅
出版者: The Japanese Society of Physical Fitness and Sports Medicine
雑誌: 体力科学 (ISSN:0039906X)
巻号頁・発行日: vol.45, no.4, pp.451-460, 1996-08-01 (Released:2010-09-30)
参考文献数: 42
被引用文献数: 2 1

スポーツ選手を対象として, 最大運動負荷に伴う白血球分画と好中球活性酸素産生能の変動を検討したところ, 以下の知見が得られた.1.運動直後に総白血球数が一過性に2倍程度上昇したが, これはリンパ球, 好中球および単球の数的増加によるものであった.好中球数は運動終了1時間後でも上昇した状態にあったが, 核左方移動は認められず, かつ分葉核好中球数の変動と相関が強かったため, 壁在プール由来の好中球動員であったと考えられる.2.リンパ球のなかでLGL (NK細胞) は運動直後に6倍も上昇しており, 終了1時間後には半減した.このようなリンパ球の数的・構成的変動が各リンパ球の機能を測定する上で誤差要因とならないように注意する必要がある.3.ルミノール依存性化学発光法を用いて単離好中球の活性酸素産生能を検討したところ, 刺激物質として貪食粒子のOZを用いた場合のみならず可溶性のPMAを用いた場合にも運動負荷に伴い有意に上昇し, かつ両者の変動には正相関が認められたことから, 単一機序で発光が増強したものと推察される.ルミノール依存性化学発光の反応機構から, 好中球の刺激に伴う脱顆粒能亢進によってMPOを介して強力な活性酸素種 (HOCl) が効率的に産生されやすくなることが示唆された.短時間の運動であっても極端に強度が高い場合には, 毒性の高い活性酸素種を生成しやすい好中球が血中に増加し, リンパ球の機能抑制や筋の炎症等の組織傷害作用を発現する可能性があり, 今後その体内動態をめぐっては, さらに踏み込んだ検討が必要である.