著者

武元 則人 丸山 博文 川村 秀樹 小松 靖弘 油田 正樹 細谷 英吉

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.9, no.2, pp.137-140, 1989-03-10 (Released:2010-04-12)

参考文献数

8

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The mitogenic activity of TJ-48 in murine lymphoid cells was examined to get a clue for the augmentation of antibody production by TJ-48.Mitogenic activity of TJ-48 was detected in spleen cells, lymphonodus cells but not in thymocytes. The effect was abolished by pretreatment with anti-Ig antibody but not affected by pretreatment with anti-Thy 1.2 antibody. Coculture of spleen cells with TJ-48 resulted in increased number of sIgM-, sIgG- or sIgD-positive cells according to FAGS analysis. The increase in mitosis by TJ-48 was abolished by the elimination of adherent cells and the readdition of adherent cells recovered the effect of TJ-48. These data indicate that TJ-48 is a B cell mitogen and the activity is T cell-independent and adherent cell-dependent. The mitogenic activity of TJ-48 may account for the augmentation of the immune response.

著者

比留間 航 駿河 康平 門倉 一成 富田 剛 関野 芳弘 小松 靖弘 木村 公彦 小野 信文

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.133, no.5, pp.487-491, 2013-05-01 (Released:2013-05-01)

参考文献数

14

被引用文献数

5 5

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Cancer is the most common cause of death in Japan. Fundamental and clinical studies on cancer were conducted from the viewpoint of Western medicine so far. However, a sustained complete remission has not been achieved yet. In order to alleviate the side effects of anticancer drugs, some traditional herbal medicines (Kampo medicines) have been prescribed to cancer patients. We have been studying on antitumor substances in medicinal herbs and found an antitumor medicinal herb named Rhus verniciflua (lacquer, Urushi in Japanese). To investigate the antitumor effect in vitro, a plant extract mixture was prepared from six medicinal herbs containing lacquer. The plant extract mixture containing lacquer (Rv-PEM) inhibited the proliferation of several mouse and human tumor cell lines. Rv-PEM had more potent inhibitory effect on the proliferation of human leukemia cell lines (MOLT-3, KG-1) than on other tumor cell lines. The IC50 values of Rv-PEM on MOLT-3 and KG-1 cells were 0.208 and 0.293 mg/mL, respectively. After treating Rv-PEM to the tumor cells, DNA fragmentation and Caspase-3 and -9 activity increased in the treated cells. The mechanisms of the inhibitory proliferation activity of Rv-PEM would involve apoptosis of human leukemia cells (MOLT-3, KG-1, K-562) by the mitochondrial pathway.

著者

新井 一郎 小松 靖弘 山浦 常 田口 進

出版者

The Japan Society for Oriental Medicine

雑誌

日本東洋医学雑誌 (ISSN:02874857)

巻号頁・発行日

vol.48, no.1, pp.31-36, 1997-07-20 (Released:2010-03-12)

参考文献数

18

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桔梗湯の消化管ホルモン分泌に対する作用を健常人において検討するとともに, 慢性膵炎患者の腹部症状に対する効果を検討した。健常人において桔梗湯エキス顆粒5g投与により血漿コレシストキニン, セクレチン濃度の上昇が認められた。また, 桔梗湯エキス顆粒7.5g/日投与により慢性膵炎患者の腹痛, 圧痛, 下痢, 軟便などの自覚症状は消失した。これらのことから, 桔梗湯はヒトにおいてコレシストキニン, セクレチンなどの消化管ホルモン分泌を介する膵外分泌刺激作用を示す可能性があり, このことを通じて, 慢性膵炎患者の腹部症状に有効であると推測された。