- 著者
- 新名 人士 谷口 佳苗 西野 有里 岩崎 憲治 高本 淳一 宮澤 敦夫 安永 卓生
- 出版者
- 一般社団法人日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.47, no.1, 2007-11-20
2 0 0 0 OA 電子顕微鏡技術の進展と相関解析
- 著者
- 岩崎 憲治
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.57, no.1, pp.66-71, 2015-02-28 (Released:2015-03-05)
- 参考文献数
- 24
Recent advances that improve direct electron detectors have revolutionized structural determination of biological molecules by cryo-electron microscopy. It has brought about the advent of near atomic resolution in single-particle reconstruction even with membrane proteins of 200~400 kDa. In fact, such high-resolution structures have allowed de novo building of atomic models. This rapid progress will change the quality and quantity of the conventional hybrid approach combining cryo-electron microscopy and X-ray crystallography or light microscopy, and computational methods such as molecular dynamics will also take on added significance to create integrated models of huge and complex macromolecules.
1 0 0 0 滑膜肉腫関連タンパク質SS18-SSX1の新規機能
1 0 0 0 OA 開閉可能な人工タンパク質ケージの構造と性質
- 著者
- 岩崎 憲治 宮崎 直幸
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.60, no.3, pp.153-156, 2020 (Released:2020-05-27)
- 参考文献数
- 7
An ultra-stable artificial protein cage was created by using TRAP (trp RNA-binding attenuation protein). Adding a gold (I) triphenylphosphine compound to a cysteine-substituted TRAP generated a cage 22 nm in diameter. The TRAP cage resisted severe conditions, such as high temperature, denaturing agents, and acidic and basic solvents. However, it was instantly disassembled when reducing agents were added to the solution. We can therefore fully control both assembly and disassembly of the TRAP cage. Cryo-EM single-particle analysis revealed a chiral pair of TRAP cages and their atomic structures, in which gold atoms acted as staples to connect the TRAP rings.