著者

木村 啓志 藤井 輝夫 酒井 康行

出版者

公益社団法人 日本薬剤学会

雑誌

薬剤学 (ISSN:03727629)

巻号頁・発行日

vol.76, no.4, pp.238-242, 2016-07-01 (Released:2017-01-01)

参考文献数

5

著者

木村 啓志

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.143, no.1, pp.39-44, 2023-01-01 (Released:2023-01-01)

参考文献数

14

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Microphysiological systems (MPSs) based on microfluidic devices are attracting attention as an alternative cell assay platform to animal experiments in drug discovery. When we use microfluidic devices for cell culture, it is possible to experiment with various culture conditions that are difficult with conventional cell culture methods, such as fabrication of microstructures for cell placement, temporal and spatial control of liquid factors and adhesive conditions, and physical stimulation by flow and expansion/contraction. MPSs, which use microfluidic technology to construct the structure and function of physiological biological tissues and organs, are being commercialized and put to practical use worldwide with the entry of venture companies and pharmaceutical companies. Although research on the practical application of MPS in Japan has lagged far behind the efforts of Western countries, the Japan Agency for Medical Research and Development (AMED) launched the MPS Development and Research Project in FY2017 and established a system for MPS commercialization through industry-government-academia collaboration. The project is characterized by the formation of a consortium involving many researchers not only from academia but also from manufacturing and pharmaceutical companies with the aim of commercializing MPS devices. By FY2021, the final year of this project, several MPSs were successfully positioned in various stages of commercialization. This paper introduces two MPSs that the author was involved in commercializing in collaboration with domestic companies within the project.

著者

木村 啓志 酒井 康行 藤井 輝夫

出版者

日本膜学会

雑誌

膜 (ISSN:03851036)

巻号頁・発行日

vol.34, no.6, pp.304-309, 2009 (Released:2015-06-14)

被引用文献数

1 1

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Conventional method of cell–based assays in life science and medical application can be difficult to duplicate in vivo situation. Microfluidics is an emerging technology with potential to provide integrated environments for cell maintenance, continuous perfusion, and monitoring. In this paper, we introduce possibility of microfluidics to become a novel cell–based assay system with its concept. Then, we show a chip–based coculture system for cytotoxicity test, as our continuous effort to develop a multi–functional micro culture system realized by integration of fluidic control and detection functionalities. The culture zone in the chip was divided into two compartments separated by a microporous membrane through which substances in culture medium can freely come-and-go to induce the mutual interactions between the cells cultured at each compartment. Performances of the chip were examined 1) monitoring of polarized transport activity of intestinal tissue models, 2) cytotoxicity model through oral intake by coculture. As a result, we conclude that microfluidics may have applications in toxicity test and drug screening.

著者

齋藤 飛鳥 Mani Ganesh Kumar 木村 啓志 槌谷 和義

出版者

公益社団法人 精密工学会

雑誌

精密工学会学術講演会講演論文集

巻号頁・発行日

vol.2019, pp.314-315, 2019

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<p>癌マーカーであるCTCの検出において,DLD法による血中細胞を種類ごとに濃縮が可能となっている.本研究ではDLD法によって濃縮されたCTCを流路内で直接的に検出可能とするpHセンサの開発を目的として,AgIO₃およびSbの二種の固体材料を用いた薄膜電極の開発を行っている.本報では比較電極であるAgIO₃層の高機能化を行った.</p>

著者

齋藤 飛鳥 Mani Ganesh Kumar 木村 啓志 槌谷 和義

出版者

公益社団法人 精密工学会

雑誌

精密工学会学術講演会講演論文集

巻号頁・発行日

vol.2018, pp.477-478, 2018

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<p>がんマーカーであるCTCの検出においてDLD法による細胞腫毎の濃縮が可能となっている.本研究ではDLD法によって濃縮されたCTCを流路内で直接的に検出可能とする,固体から成るpHセンサの開発を目的として,AgIO3およびSbの二種の固体材料を用いた薄膜電極の開発を行っている.本報では,バッファ層の探索により,電極の耐久性とセンシング感度の向上を行った.</p>

著者

高橋 翼 中村 寛子 木村 啓志

出版者

一般社団法人 日本機械学会

雑誌

日本機械学会論文集 (ISSN:21879761)

巻号頁・発行日

vol.83, no.850, pp.16-00560-16-00560, 2017 (Released:2017-06-25)

参考文献数

16

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Nowadays, in vitro fertilization (IVF) of mammalian embryos is an essential technique in the reproductive technology and other related life science fields. However, the fertility rate by this technique is still less than 25%. Therefore, a novel in vitro fertilization method which obtains high fertility rate has been highly desired to the reproductive technology. In this study, we proposed and developed an in vitro embryo production device which allows three steps of sperm selection, fertilization and culture on a microdevice. To realize this concept, we integrated the sperm sorting function combining with swim-up and swim-down methods onto the device. To evaluate the device functions, sperm sorting experiment, in vitro fertilization experiment, and embryo culture experiment have been carried out. As results, we concluded that the device has ability to produce high quality embryos by integrating the sperm sorting function. This concept will open and enhance the management of in vitro fertilized embryos for assisted reproductive technology, livestock breeding, and fundamental stage research by further development.