著者

池崎 和海 芝口 翼 杉浦 崇夫 宮田 浩文

出版者

一般社団法人日本体力医学会

雑誌

体力科学 (ISSN:0039906X)

巻号頁・発行日

vol.66, no.5, pp.345-354, 2017-10-01 (Released:2017-09-29)

参考文献数

27

被引用文献数

1

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Although icing treatment has been well accepted as aftercare in sports fields, the detailed mechanisms of the treatment is not fully understood. In this study, we investigated the effect of icing treatment on the recovery process of rat plantaris muscles with artificially induced muscle damage. Sixty male Wistar rats (8-weeks-old) were randomly assigned to three groups; control (CTL), bupivacaine-injected (BPVC), and icing treatment after BPVC (ICE). Icing treatment was applied for 20 min immediately after BPVC, and the treatment was used once per day for 3 days. The plantaris muscles were removed at 3, 7, 15, and 28 days after the muscle damage, then immunohistochemical and real time RT-PCR analysis were performed. In histochemical analysis, although significant changes were found in the relative muscle weight, cross-sectional area of muscle fiber, percentage of muscle fiber with central nuclei, and expressed immature myosin heavy chain isoforms after muscle damage, as compared to the CTL group, no differences were found between BPVC and ICE groups. In mRNA expression analysis, the ICE group had a significantly lower value of MyoD than the BPVC group at 3 days after the damage. Expression of IL-6 mRNA, which relates to muscle inflammation, indicated significantly higher value in BPVC, but not in ICE, than CTL groups at 7days after the damage. Furthermore, BKB2 receptor, which relates to acute muscle soreness, indicated a significantly higher expression in BPVC than ICE groups at 3 days after the damage. These results suggest that icing treatment is effective to suppress muscle inflammation and soreness at an early stage of recovery from damage, but not effective for muscle regeneration at a later stage.

著者

柳川 洋一 杉浦 崇夫 阪本 敏久 岡田 芳明

出版者

Japanese Association for Acute Medicine

雑誌

日本救急医学会雑誌 (ISSN:0915924X)

巻号頁・発行日

vol.17, no.2, pp.62-66, 2006-02-15 (Released:2009-03-27)

参考文献数

9

被引用文献数

3 3

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症例は33歳の男性。重症破傷風に罹患し,筋弛緩剤と併用でデクスメデトミジンやプロポフォールで治療を行っていたが,副作用や強直抑制効果が乏しかったなどの理由で,21日間のマグネシウム大量投与療法を行った。同療法を施行中はある程度意思疎通を保つことができ,適当な筋弛緩作用と交感神経抑制作用も得ることができた。リハビリテーション後,第88日に退院となった。マグネシウム大量投与療法による破傷風の治療報告は本邦では初めてであるが,廉価で意思疎通がある程度保て,筋弛緩,交感神経抑制作用を有し,破傷風治療に有用と考えられた。

著者

杉浦 崇夫 的場 秀樹 村上 悳

出版者

The Japanese Society of Physical Fitness and Sports Medicine

雑誌

体力科学 (ISSN:0039906X)

巻号頁・発行日

vol.32, no.1, pp.17-24, 1983-02-01 (Released:2010-12-10)

参考文献数

32

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Sex difference in enzyme activities of the skeletal muscle were examined in rats aged 10 and 35 weeks. At 10 and 35 weeks of age, animals were anesthetized with ether and weighed. M, gastrocnemius, m.extensor digitorum longus and m.soleus were removed from both legs under pentobarbital anethesia and weighed. These muscles were used for the determination of myosin ATPase activity, phosphofruktokinase (PFK) activity, succinate dehydrogenase (SDH) activity and non-collagenous protein (NCP) content.The results were summarized as follows:1) Thirty-five week-old rats had heavier body and muscle weights than 10-weekold rats in both sexes and males had significantly heavier body and muscle weights than females at both 10 and 35 weeks of age.2) Similar tendency was observed with regard to total NCP content. Furthermore, it was found that total NCP content was positively correrated to muscle weight (r=0.871, r=0.909 and r=0.871 in m. gastrocnemius, m.extensor digitorum longus and m.soleus, respectively) . However, no significant difference in NCP content per wet weight was found between both sexes and between different age groups.3) Myosin ATPase activity tended to be lower at 35 weeks of age than at 10 weeks of age, the tendency being the most prominent in the gastrocnemius muscle of male rat (p<0.05) . However, no significant sex difference in myosin ATPase activity was observed in three muscle examined in both age groups.4) Although not statistically significant, mean PFK activity was slightly lower in 35-week-old rats than in 10-week-old rats, and there was no sex difference in PFK activity at both weeks of age.5) SDH activity was significantly lower in 35-week-old rats than in 10-week-old rats except that there was no significant age difference in the gastrocnemius muscle of males. There was no significant sex difference in SDH activity in both age groups with an exception of the extensor digitorum longus muscle from 35-week-old rats, where males had significantly higher SDH activity than females.