著者

高野 幹久 湯元 良子

出版者

日本膜学会

雑誌

膜 (ISSN:03851036)

巻号頁・発行日

vol.36, no.4, pp.145-153, 2011 (Released:2011-12-13)

参考文献数

25

被引用文献数

3 3

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The lung is expected as a promising administration route for protein and peptide drugs. However, information concerning alveolar absorption mechanisms of these high molecular weight drugs is lacking. The alveolar region of the lung is lined with a continuous epithelium comprising of type I and type II epithelial cells, and squamous type I cells cover 90~95% and cuboidal type II cells cover 5~10% of the alveolar surface area. Using primary cultured alveolar type II and transdifferentiated type I cells, however, it was found that type II cells would play a significant role in albumin and insulin endocytosis from the alveolar space. Functional and siRNA studies suggest that albumin is taken up by clathrin-mediated endocytosis in type II cells, while clathrin- and caveolae-independent but dynamin-dependent pathway is involved in insulin endocytosis. The approach to enhance insulin uptake by the alveolar epithelial cells and absorption from the lung is also discussed.

著者

湯元 良子 田川 茉希 永井 純也 酒井 正彦 桑田 直治 木平 健治 石野 岳志 平川 勝洋 高野 幹久

出版者

一般社団法人日本医療薬学会

雑誌

医療薬学 (ISSN:1346342X)

巻号頁・発行日

vol.34, no.10, pp.951-955, 2008 (Released:2010-02-07)

参考文献数

19

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Burow’s solution,a hospital preparation,exhibits antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and other microorganisms that are commonly observed in chronic supprative otitis media.However,it takes several days to prepare Burow’s solution by the method commonly employed in Japan and it is difficult to ensure constant quality.With this in mind,we examined the pharmaceutical and pharmacological characteristics of Burow’s solution and developed a new prescription for it (Neo-Burow’s solution) having an aluminum acetate base.The new prescription takes only a few hours to prepare,is convenient,and ensures a solution of constant quality.In this study,we examined the clinical effects of Neo-Burow’s solution in external and internal otitis.We prepared NeoBurow’s solution in the Department of Pharmacy and used it for 13 patients in whom commercially available antibiotics had had no effect,in the Department of Otorhinolaryngology of Hiroshima University Hospital.Nine of the patients completely recovered after treatment with Neo-Burow’s solution,and it had some effect in 1 patient but was completely ineffective in the remaining 3 patients.No adverse reactions were observed.Our results indicated that Neo-Burow’s solution was effective and safe in the treatment of chronic suppurative otitis,even that accompanied by MRSA.We have received a lot of inquiries about Neo-Burow’s solution from pharmacies and other hospital facilities,most of them relating to its preparation and preservation.Our manuscript includes a summary of the inquiries and the answers given to them.

著者

湯元 良子 高野 幹久

出版者

一般社団法人日本医療薬学会

雑誌

医療薬学 (ISSN:1346342X)

巻号頁・発行日

vol.28, no.5, pp.484-488, 2002-10-10 (Released:2011-03-04)

参考文献数

7

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Clinical research as well as basic research is one of the important aspects for the development of clinical pharmacy. In clinical pharmacy research, like research in medicine, it is desirable to find and investigate problems on clinical pharmacotherapy and to utilize the findings of such results in the therapy. In order to promote such clinical pharmacy research, collaboration between hospital or community pharmacies and pharmaceutical universities is very important, because the former usually does not have enough space, equipment, or pharmacists to do the research, and the latter usually does not have chance to find the problems in clinical pharmaco-therapy. In the present study, we carried out a questionnaire survey concerning clinical pharmacy research, in order to clarify the present situations and needs in hospital or community pharmacy in Hiroshima prefecture. The results indicated that many pharmacists in hospital as well as community pharmacy feel that it is necessary to do clinical pharmacy research to understand and clarify the problems they found in clinical pharmacotherapy. In addition, though many of them are either not doing or can not do clinical pharmacy research under the present situation, they are interested in collaborating with pharmaceutical universities. These results offer important information in promoting clinical pharmacy research based on the collaboration between hospital or community pharmacies and pharmaceutical universities.

著者

石橋 祐子 村上 照夫 湯元 良子 酒井 正彦 新谷 洋通 板羽 秀之 神辺 眞之 益田 慎 夜陣 紘治 木平 健治 高野 幹久

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.124, no.11, pp.833-840, 2004 (Released:2004-11-01)

参考文献数

15

被引用文献数

6 6

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Burow's solution, or aluminum acetate solution, is effective in inhibiting various microorganisms including methicillin-resistant Staphylococcus aureus (MRSA) that are commonly observed in chronic suppurative otitis media. It takes several days to prepare Burow's solution using aluminum sulfate, and the pharmaceutical properties of the solution are not fully understood. In this study, the effect of storage (5 months) of Burow's solution prepared according to the Teine-Keijin Hospital manual on its pharmaceutical properties and antibacterial activities was examined. We also attempted to develop a rapid preparation method of aluminum acetate (or 1.7% aluminum) solution using two commercially available compounds of aluminum acetate basic (Al2O(CH3CO2)4, Al(OH)(CH3CO2)2). The properties of Burow's solution, pH, osmolarity and antibacterial activity, were the same among different preparations and its storage for 5 months at 4°C had no effect on these properties. The antibacterial potency of Burow's solution was dependent on aluminum concentration and its antibacterial potency against S. aureus and several MRSA strains was of the same magnitude. In a rapid preparation, aluminum acetate basic was mixed with appropriate amounts of tartaric acid and acetic acid, and the suspension was boiled for 2—2.5 hr until dissolved. The rapidly prepared aluminum acetate solution showed the same pharmaceutical properties and antibacterial activities as those of Burow's solution. The newly developed preparation method for aluminum acetate solution is expected to be convenient and feasible for hospital treatment of chronic suppurative otitis media.

著者

高野 幹久 永井 純也 湯元 良子 永井 純也 湯元 良子

出版者

広島大学

雑誌

基盤研究(B)

巻号頁・発行日

2007

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初代培養ラット肺胞上皮II型・I型細胞を用いて、アルブミンの輸送について比較解析したところ、取り込みはII型のほうがI型に比べて高く、またクラスリン介在性エンドサイトーシスによることが明らかとなった。一方、インスリンの取り込みは両細胞で同程度であり、クラスリンの関与は小さかった。ラット肺胞上皮II型由来のRLE-6TN細胞においてもインスリンはエンドサイトーシスによって取り込まれ、その取り込みはカチオン性ポリアミノ酸によって促進された。カチオン性ポリアミノ酸の効果は、ラットを用いたin vivoインスリン経肺投与実験でも認められた。これらの知見は新たな経肺投与製剤の開発のための情報として重要である。