- 著者
- YANG Ling AKAO Teruaki KOBASHI Kyoichi HATTORI Masao
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- Biological & pharmaceutical bulletin (ISSN:09186158)
- 巻号頁・発行日
- vol.19, no.5, pp.701-704, 1996-05-15
- 参考文献数
- 15
- 被引用文献数
- 1 8
Bifidobacterium sp. strain SEN was isolated and characterized by hydrolytic conversion of sennosides to sennidins (Akao et al., Appl. Environ. Microbiol., 60, 1041 (1994)). The sennoside-hydrolyzing capacity of the strain SEN was disappeared following the addition of glucose to the media in spite of good bacterial growth and potent activity hydrolyzing p-nitrophenyl β-D-glucopyranoside (pNPG). In a fructose-containing medium, no such suppressing effect was shown. Following a 10 h incubation in 50 mM potassium phosphate buffer (pH 7.4), the sennoside-hydrolyzing activity of the bacterium increased, dose-dependently, with the addition of sennoside B. Inhibition of the substrate-induced increase in sennoside-hydrolyzing activity was observed following the addition of some antibiotics (chloramphenicol, streptomycin, and rifampicin). In particular, chloramphenicol completely inhibited the increase of sennoside-hydrolyzing activity while 38% pNPG-hydrolyzing activity remained. It is suggested that the strain SEN produces two different β-glucosidases of which the sennoside-hydrolyzing enzyme is inducible. In addition, the glucosides pNPG, esculin, salicin, or amygdalin stimulated the induction of the sennoside β-glucosidase, but less markedly than sennoside. Sennidin A or sugars (glucose, fructose, cellobiose, or maltose) did not induce the enzyme.
- 著者
- 谷川 聖明 GOTO Hirozo NAKAMURA Norio TANAKA Nobumitsu HATTORI Masao ITOH Takashi TERASAWA Katsutoshi
- 出版者
- 和漢医薬学会
- 雑誌
- 和漢医薬学雑誌 (ISSN:13406302)
- 巻号頁・発行日
- vol.16, no.2, pp.45-50, 1999-07-20
- 被引用文献数
- 6
桂皮の血流改善作用については古くから知られており,これに関連した報告はあるものの,その詳細な検討はなされていない。今回我々はマグヌス法を用いて,ラット胸部大動脈輪状標本における桂皮含有クンニンの血管作動性について検討した。桂皮含有タンニンは,プロスタグランディンF_<2α>(PGF_<2α>)の血管収縮に対し,内皮保存血管において濃度依存性に血管弛緩作用が認められた。しかし,内皮除去血管及ぴN^G-nitro-l-argininemethyl ester(L-NAME)前処置内皮保存血管においては,血管弛緩作用はほぼ消失した。以上より,桂皮含有タンニンの血管弛緩作用は内皮依存性であることが明らかとなった。桂皮含有タンニンをさらに二量体から五量体までのタンニン画分に分取し検討したところ,二量体以上の重合したタンニンにおいて血管弛緩作用が認められた。また,重合度が増すに従い血管弛緩作用はより低い濃度で発揮され,作用も増強されることが明らかとなった。
- 著者
- Namba Tsuneo HATTORI MASAO TSUNEZUKA MASA YAMAGISHI TAKAYOSHI KONISHI KENICHI
- 出版者
- 日本生薬学会
- 雑誌
- 生薬学雑誌 (ISSN:00374377)
- 巻号頁・発行日
- vol.36, no.3, pp.222-227, 1982-09-20
- 被引用文献数
- 1
By the agar dilution technique, magnolol and honokiol, the components of Magnoliae Cortex, were demonstrated to have antibacterial activity against various strains of gram-positive bacteria, including Streptococcus sp., Staphylococcus sp., Peptococcus sp., Peptostreptococcus sp., Micrococcus sp., Lactobacillus sp., Eubacterium sp., Bifidobacterium sp. and Clostridium sp. with minimal inhibitory concentrations of 12. 5-25 μg/ml, but not against those of gram-negative bacteria. Both compounds were strongly bactericidal against Streptococcus mutans, Streptococcus pyogenes, Streptococcus pneumoniae, Staphylococcus aureus etc., which were killed within 2. 5 mins in contact with the solutions of 100μg/ml.
- 著者
- Nawawi As'ari Ma Chao-mei Nakamura Norio HATTORI Masao KUROKAWA Masahiko SHIRAKI Kimiyasu KASHIWABA Noriaki ONO Minoru
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- Biological & pharmaceutical bulletin (ISSN:09186158)
- 巻号頁・発行日
- vol.22, no.3, pp.268-274, 1999-03-15
- 被引用文献数
- 3 34
By screening water and MeOH extracts of 30 Chinese medicinal plants for their anti-herpes simplex virus (HSV)-1 activity, a MeOH extract of the root tubers of Stephania cepharantha HAYATA showed the most potent activity on the plaque reduction assay with an IC_<50> value of 18.0 μg/ml. Of 49 alkaloids isolated from the MeOH extract, 17 alkaloids were found to be active against HSV-1,including 13 bisbenzylisoquinoline, 1 protoberberine, 2 morphinane and 1 proaporphine alkaloids, while benzylisoquinoline and hasubanane alkaloids were inactive. Although N-methylcrotsparine was active against HSV-1,as well as HSV-1 thymidine kinase deficient (acyclovir resistant type, HSV-1 TK^-)and HSV-2 (IC_<50> values of 8.3,7.7 and 6.7 μg/ml, respectively), it was cytotoxic. FK-3000 was found to be the most active against HSV-1,HSV-1 TK^- and HSV-2 (IC_<50> values of 7.8,9.9 and 8.7 μg/ml) with in vitro therapeutic indices of 90,71 and 81,respectively. FK-3000 was found to be a promising candidate as an anti-HSV agent against HSV-1,acyclovir (ACV) resistant-type HSV-1 and HSV-2.
- 著者
- Gao Jiang-Jing Min Byung-Sun Ahn Eun-Mi NAKAMURA Norio LEE Hyeong-Kyu HATTORI Masao
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- Chemical & pharmaceutical bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.50, no.6, pp.837-840, 2002-06-01
- 被引用文献数
- 6 119
Three new lanostante-type triterpene aldehydes, named lucialdehydes A-C (1-3), were isolated from the fruiting bodies of Ganoderma lucidum, together with ganodermanonol (4), ganodermadiol (5), ganodermanondiol (6), ganodermanontriol (7), ganoderic acid A (8), ganoderic acid B8 (9), and ganoderic acid C1 (10). The structures of the new triterpenes were determined as (24E)-3β-hydroxy-5α-lanosta-7,9(11),24-trien-26-al (1), (24E)-3,7-dioxo-5α-lanosta-8,24-dien-26-al (2), and (24E)-3β-hydroxy-7-oxo-5α-lanosta-8,24-dien-26-al (3), respectively, by spectroscopic means. The cytotoxicity of the compounds isolated from the ganoderma mushroom was tested in vitro against Lewis lung carcinoma (LLC), T-47D, Sarcoma 180, and Meth-A tumor cell lines. Lucialdehydes B, C (2, 3), ganodermanonol (4) and ganodermanondiol (6) showed cytotoxic effects on tested tumor cells. Of the compounds, lucialdehyde C (3) exhibited the most potent cytotoxicity against LLC, T-47D, Sarcoma 180, and Meth-A tumor cells with ED_<50> values of 10.7, 4.7, 7.1, and 3.8 /μg/ml, respectively.