著者

Katsuhito SONE Hideo AKIYOSHI Junichiro SHIMIZU Zheng CAO Yijyun LI Toshiyuki TANAKA Akiyoshi HAYASHI Shunji SUGII Fumihito OHASHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0255, (Released:2013-01-17)

被引用文献数

1 6

---

Pulmonary surfactant protein A (SP-A) is used as a biomarker to understand the clinical features of pulmonary diseases and associated prognostic indices in human medicine. This study was conducted to investigate whether or not serum SP-A concentration can be used as a biomarker for identifying pulmonary parenchymal diseases in dogs. Thirty-two dogs with pulmonary parenchymal diseases, 34 with nonrespiratory diseases and 57 healthy dogs were included. Serum SP-A concentration was measured in all dogs using sandwich enzyme linked immunosorbent assay with an anti-dog SP-A polyclonal antibody. Median serum SP-A concentration in healthy dogs was <2.0 ng/ml, whereas that in dogs with aspiration pneumonia (n=11), primary lung tumors (n=9) and blunt traumatic lung injury (BTLI; n=12) was 3.1, 7.2 and 2.6 ng/ml, respectively; these values were significantly higher than those in healthy dogs. The serum SP-A concentration in dogs with nonrespiratory diseases was comparable with that in healthy dogs. No correlation was observed between the serum SP-A and plasma C-reactive protein concentrations in dogs with aspiration pneumonia and BTLI. There was a significant correlation between the serum SP-A concentration and thoracic radiographic changes in dogs with BTLI. These findings suggest that the serum SP-A concentration may be a useful clinical biomarker of alveolar damage that can be used for differential diagnosis of pulmonary parenchymal diseases and nonrespiratory diseases in dogs.

著者

Takaharu ITAMI Kodai KAWASE Naomichi TAMARU Tomohito ISHIZUKA Jun TAMURA Kenjirou MIYOSHI Mohammed A. UMAR Hiroki INOUE Kazuto YAMASHITA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0208, (Released:2013-01-07)

被引用文献数

5 14

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Tramadol is an atypical opioid analgesic widely used in small animal practice. This study was designed to determine the effect of a single intravenous (IV) dose of tramadol on the minimum alveolar concentration (MAC) of sevoflurane in dogs. Six beagle dogs were anesthetized twice to determine the sevoflurane MAC with or without an administration of tramadol (4 mg/kg, IV) at 7 days interval. The sevoflurane MAC was determined using a tail clamp method in each dog ventilated with positive pressure ventilation. The tramadol administration produced a significant reduction in the sevoflurane MAC by 22.3 ± 12.2% (1.44 ± 0.28% with tramadol versus 1.86 ± 0.30% without tramadol, P=0.010). This MAC reduction had been determined from 122 ± 19 to 180 ± 41 min following the tramadol administration. During this period, the plasma concentrations of tramadol and its metabolite, O-desmethyltramadol (M1), decreased from 429 ± 64 to 332 ± 55 ng/ml and from 136 ± 24 to 114 ± 68 ng/ml, respectively, but these changes were not statistically significant. There was no significant difference in heart rate, mean arterial blood pressure and SpO2 between the control and tramadol treatment. The dogs that received tramadol treatment sometimes breathed spontaneously. Therefore, their respiratory rates significantly increased, and PETCO2 decreased during the MAC determination. In conclusion, the single IV dose of tramadol produced a significant reduction in the sevoflurane MAC in dogs.

著者

Hiroko MIZOOKU Yumiko KAGAWA Kazuya MATSUDA Minoru OKAMOTO Hiroyuki TANIYAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0447, (Released:2012-12-28)

被引用文献数

6

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Histological and immunohistochemical evaluation of lobular dissecting hepatitis (LDH) were performed in nine American cocker spaniel dogs. Histological examination showed diffuse fibrosis with weak inflammatory reaction of extensive neutrophils, macrophages, lymphocytes and plasma cells. Immunohistochemical examination revealed the myofibroblastic cells positive to anti-α-smooth muscle actin and anti-α-vimentin antibodies produced reticular and collagen fibers and brought about the dissection of hepatic cords and diffuse disappearance of hepatocytes. Reticular fibers invading between hepatocytes and the surrounding small group of hepatocytes were strongly positive for anti-collagen type Ⅲ and anti-collagen type Ⅳ antibodies. The positivity to anti-fibronectin and anti-laminin antibodies was frequently continuous on the basement membrane of the sinusoids of the remaining hepatic cords and between the hepatocytes. Positive findings for anti-E-cadherin antibody were not observed between the hepatocytes showing the positive findings to anti-collagen type Ⅲ and anti-collagen type Ⅳ antibodies. These results may explain the expression of fibronectin and laminin that occurs prior to the invasion of reticular fibers between the hepatocytes. The present study further suggests that expression of an extracellular matrix mainly containing of fibronectin and laminin between the hepatocytes and proliferation of collagen fibers and reticular fibers has a major role in the rupture of the hepatic cords and disappearance of hepatocytes.

著者

Kanchana POONSUK Rungtip CHUANCHUEN

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.12, pp.1575-1582, 2012 (Released:2012-12-28)

参考文献数

34

被引用文献数

6 19

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As study of multidrug efflux pumps is a crucial step for development of efflux pump inhibitors for treatment of Pseudomonas aeruginosa infection, the objective of this study was to examine the contribution of the MexXY multidrug efflux systems and other chromosomal mechanisms in aminoglycoside (AMG) resistance in P. aeruginosa isolated from dogs and cats. Thirteen Pseudomonas aeruginosa isolates from canine and feline infections were examined for contribution of the MexXY multidrug efflux pump and four other chromosomally-encoded genes including PA5471, galU, nuoG and rplY to AMG resistance. All the isolates were resistant to multiple AMGs and expressed mexXY. Deletion of mexXY caused 2- to 16-fold reduction in AMG MICs. Overproduction of MexXY did not fully account for the observed AMG resistance. No good correlations were detected between MexXY transcription level and AMG MICs. While no mutations were found in mexZ, PA5471 expression varied and its impact on MexXY expression and AMG resistance is diverse. No mutations were found in galU. Only two isolates carried a single base change G-367-T in rplY. Complete transcription of nuoG was detected in all the isolates. In conclusion, the MexXY multidrug efflux pump plays a role in AMG resistance in the dog and cat P. aeruginosa isolates, while disruption of nuoG, rplY and galU did not have a significant impact. These results indicate the existence of uncharacterized AMG-resistance mechanisms.

著者

Mari VAINIONPÄÄ Marja RAEKALLIO Elina TUHKALAINEN Hannele HÄNNINEN Noora ALHOPURO Maija SAVOLAINEN Jouni JUNNILA Anna HIELM-BJÖRKMAN Marjatta SNELLMAN Outi VAINIO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.12, pp.1539-1544, 2012 (Released:2012-12-28)

参考文献数

30

被引用文献数

1 15

---

The objective of this study was to compare the method of thermography by using three different resolution thermal cameras and basic software for thermographic images, separating the two persons taking the thermographic images (thermographers) from the three persons interpreting the thermographic images (interpreters). This was accomplished by studying the repeatability between thermographers and interpreters. Forty-nine client-owned dogs of 26 breeds were enrolled in the study. The thermal cameras used were of different resolutions-80 × 80, 180 × 180 and 320 × 240 pixels. Two trained thermographers took thermographic images of the hip area in all dogs using all three cameras. A total of six thermographic images per dog were taken. The thermographic images were analyzed using appropriate computer software, FLIR QuickReport 2.1. Three trained interpreters independently evaluated the mean temperatures of hip joint areas of the six thermographic images for each dog. The repeatability between thermographers was >0.975 with the two higher-resolution cameras and 0.927 with the lowest resolution camera. The repeatability between interpreters was >0.97 with each camera. Thus, the between-interpreter variation was small. The repeatability between thermographers and interpreters was considered high enough to encourage further studies with thermographic imaging in dogs.

著者

Hak-Hyun RYU Byung-Jae KANG Sung-Su PARK Yongsun KIM Gyu-Jin SUNG Heung-Myong WOO Wan Hee KIM Oh-Kyeong KWEON

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.12, pp.1617-1630, 2012 (Released:2012-12-28)

参考文献数

54

被引用文献数

21 99

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Previous animal studies have shown that transplantation of mesenchymal stem cells (MSCs) into spinal cord lesions enhances axonal regeneration and promotes functional recovery. We isolated the MSCs derived from fat, bone marrow, Wharton’s jelly and umbilical cord blood (UCB) positive for MSC markers and negative for hematopoietic cell markers. Their effects on the regeneration of injured canine spinal cords were compared. Spinal cord injury was induced by balloon catheter compression. Dogs with injured spinal cords were treated with only matrigel or matrigel mixed with each type of MSCs. Olby and modified Tarlov scores, immunohistochemistry, ELISA and Western blot analysis were used to evaluate the therapeutic effects. The different MSC groups showed significant improvements in locomotion at 8 weeks after transplantation (PP<0.05). Transplanted MSCs survived for 8 weeks and reduced IL-6 and COX-2 levels, which may have promoted neuronal regeneration in the spinal cord. Our data suggest that transplantation of MSCs promotes functional recovery after SCI. Furthermore, application of UCSCs led to more nerve regeneration, neuroprotection and less inflammation compared to other MSCs.

著者

Kenji TAKATA Yoshiaki HIKASA Hiroshi SATOH

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.12, pp.1545-1550, 2012 (Released:2012-12-28)

参考文献数

27

被引用文献数

2

---

This study elucidated differences in predisposition to the gastrointestinal adverse effects of ketoprofen between young and adult cats. Ketoprofen was administered subcutaneously (2.0 mg/kg, s.c.) once a day for 3 days. The animals were sacrificed 24 hr after final injection to allow examination of gastrointestinal mucosal lesions. Ketoprofen caused gastric lesions in adult cats (>6 months) but not in young cats (<3 months). Ketoprofen caused more severe small intestinal lesions in adult cats than in young cats. In the study of prevention of lipopolysaccharide (LPS)-induced hyperthermia using ketoprofen, young and adult cats of both sexes were administered LPS (0.3 μg/kg, intravenously), and body temperature was measured 24 hr later. Ketoprofen was administered subcutaneously 30 min before LPS injection. LPS-induced hyperthermia was almost completely inhibited by pretreatment with ketoprofen in both adult and young cats. In the pharmacokinetics of ketoprofen, plasma concentrations were analyzed by high-performance liquid chromatography. No significant differences were observed in plasma concentrations of two mirror-image R(-) and S(+) ketoprofen between young and adult cats from 0.5–4 hr after injection. As observed in a previous study using flunixin, the degree of gastrointestinal damage was unrelated to plasma concentrations of ketoprofen. The results of this study demonstrated that ketoprofen is safer for use in young cats than in adult cats from the viewpoint of gastrointestinal adverse effects.

著者

Okjin KIM Kyung-Suk KIM

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.67, no.1, pp.121-123, 2005 (Released:2005-02-05)

参考文献数

7

被引用文献数

8 13 19

---

A male 8-year-old Yorkshire Terrier dog with unilateral cryptorchism was presented for investigation of reduced appetite and multifocal alopecia. Abdominal sonography and radiography demonstrated abnormal enlargement of left testicle in abdominal cavity. Both of the retroperitoneal cryptorchid testicle and the other contralateral testicle were removed surgically. The concentrations of testosterone and estradiol in blood collected from the jugular vein and the two spermatic veins were evaluated and the results revealed high estradiol concentration. The retroperitoneal cryptorchid testicle was enlarged, firm, bulging sphere mass. The cut surface revealed homogeneous white color and lobulation by septa. The contralateral testicle in scrotum showed atrophic testicle and enlarged epididymis. Histopathologically, the retroperitoneal cryptorchid testicle was diagnosed as seminoma. We thought that hyperesterogenemia and alopecia in this case was probably related with his seminoma, although high correlations between Sertoli cell tumor and alopecia have been reported. To our knowledge, this report may be a rare case of seminoma with hyperesterogenemia and alopecia.

著者

Ji-Seon YOON Tomohiko MINAMI Yasuko TAKIZAWA Maiko SEKIGUCHI Atsushi YABUZOE Kaori IDE Koji NISHIFUJI Toshiroh IWASAKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.72, no.11, pp.1513-1516, 2010 (Released:2010-11-30)

参考文献数

12

被引用文献数

1 2 1

---

Two dogs of juvenile-onset skin diseases with involvement of extremities were examined by histopathological, immunohistochemical and ultrastructural analyses. Clinically, both cases showed alopecia and crusts on the face and extremities. Case 1 showed histopathology of dermo-epidermal separation. Ultrastructural analysis revealed that the clefts were recognized between hemidesmosomes and lamina densa. In case 2, histopathology showed follicular atrophy, vacuolar degeneration at lower epidermis and masseter muscle degeneration, without remarkable ultrastructural abnormalities in basement membrane zone of the skin. Thus, the findings in case 1 were compatible to those in junctional epidermolysis bullosa, while those in case 2 were compatible to dermatomyositis-like disease. Combination of histopathological and ultrastructural analyses was useful to distinguish the diseases in two dogs.

著者

Ikuo INOUE Sanae SHIBATA Tsuneo FUKATA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0489, (Released:2012-12-19)

被引用文献数

1 4 2

---

The aim of this study was to determine the antimicrobial resistance of E. coli isolated from the uteri of bitches with pyometra, and 38 E. coli isolates were used. The antimicrobials used were ampicillin (ABPC), amoxicillin/clavulanic acid, gentamicin, minocycline, cefazolin, levofloxacin (LVFX), trimethoprim-sulfamethoxazole (ST) and fosfomycin (FOM). Resistance to ABPC occurred most frequently, followed by LVFX and ST. Multi-drug resistance, defined as resistance against 3 or more classes of antimicrobials, was found in 23.7% of all isolates. Nine out of 13 resistant strains were multi-drug resistant, but no strain was found to be resistant to FOM. This suggests that FOM should be administrated for E. coli from pyometra.

著者

Hitoshi HATAI Mitsuru IBA Daisuke KOJIMA Chun-Ho PARK Yasuhiko TSUCHIDA Toshifumi OYAMADA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0446, (Released:2012-12-10)

被引用文献数

1 6

---

A 13-year-old castrated male Labrador retriever dog presented with a mass caudal to the first molar of his left mandible. Although the tumor was excised, a recurrent tumor was detected one month later and resected. Both tumors displayed invasive growth and were composed of neoplastic proliferation arranged in irregular lobules, nests and cords continuous with mucosal epithelium. The most prominent feature of the tumors was the presence of many proliferating spindle cells admixed with palisading basal-like cells, acanthocytes and stellate cells. In immunohistochemical examinations, the spindle cells were found to be positive for vimentin; cytokeratin AE1/AE3, 5/6, 14 and 19; and p63. The other neoplastic cells were positive for all of these markers shown above except vimentin. Based on these findings, the tumors were diagnosed as spindle cell ameloblastic carcinoma.

著者

Kiwamu HANAZONO Shinya FUKUMOTO Kazuko HIRAYAMA Kazuaki TAKASHIMA Yoshihisa YAMANE Masahiro NATSUHORI Tsuyoshi KADOSAWA Tsuyoshi UCHIDE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.11, pp.1477-1482, 2012 (Released:2012-12-01)

参考文献数

22

被引用文献数

3 9 1

---

Gastrointestinal stromal tumor (GIST), a mesenchymal neoplasm affecting the gastrointestinal tract, shows a variety of clinical behaviors from inactive benign to aggressive malignant in dogs. In this study, the feasibility of using clinically significant ultrasonographic features to predict the metastatic potential of canine GIST was investigated through comparison with actual metastatic incidence and findings of malignancy obtained by postoperative pathological examination. Ultrasonographic features, including large tumor size, irregular margin and heterogeneous internal echogenicity with large hypoechoic areas, related closely with the presence of metastasis as well as a high-risk ranking by the human classification system according to pathological findings. Based on these ultrasonographic features, the potential of metastasis in canine GIST could be preoperatively predicted.

著者

Haengbok CHEON Wooshin CHOI Youngjae LEE Donghoon LEE Juhyung KIM Ji-Houn KANG Kijeong NA Jinhwa CHANG Dongwoo CHANG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.11, pp.1461-1467, 2012 (Released:2012-12-01)

参考文献数

30

被引用文献数

5 19 1

---

The aim of this study was to assess age-related changes and anatomic variation in trabecular bone mineral density (tBMD) using quantitative computed tomography (QCT) in normal cats. Seventeen normal cats were included in this study and divided into the following 3 age groups:6 years (n=3). A computed tomographic scan of each vertebra from the 12th thoracic to the 7th lumbar spine and the pelvis was performed with a bone-density phantom (50, 100 and 150 mg/cm3, calcium hydroxyapatite, CIRS phantom®). On the central transverse section, the elliptical region of interest (ROI) was drawn to measure the mean Hounsfield unit (HU) value. Those values were converted to equivalent tBMD (mg/cm3) by use of the bone-density phantom and linear regression analysis (r2 >0.95). The mean tBMD value of the thoracic vertebrae (369.4 ± 31.8 mg/cm3) was significantly higher than that of the lumbar vertebrae (285 ± 58.1 mg/cm3). The maximum tBMD occurred at the T12, T13 and L1 levels in all age groups. There was a statistically significant difference in the mean tBMD value among the 3 age groups at the T12 (PPP=0.013), respectively. The present study suggests that age-related changes and anatomic variation in tBMD values should be considered when assessing tBMD using QCT in cats with bone disorders.

著者

Daisuke HASEGAWA Kazuyuki UCHIDA Takayuki KUWABARA Shunta MIZOGUCHI Naoko YAYOSHI Michio FUJITA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.11, pp.1517-1521, 2012 (Released:2012-12-01)

参考文献数

20

被引用文献数

1 8

---

A 9 year-old, neutered, male French Bulldog showing cluster seizures was diagnosed with a glioma in the right piriform cortex by MRI. Hypofractionated radiation therapy (RT) was performed using a linear accelerator. Although the lesion had involuted significantly at 2 months after RT, recurrence was observed at 4 months after RT. Chemotherapy was started using CCNU (60 mg/m2 every 6–9 weeks) and was continued for one year. Follow-up MRI revealed involution of the lesion and the intervals of CCNU were increased to every 9–14 weeks. Two years after the first presentation, the dog suffered status epilepticus, followed by deficits of left sided postural reaction with cognitive dysfunction. The dog died on day 910, and histopathological diagnosis confirmed anaplastic oligodendroglioma.

著者

Lee-Shuan LIN Yuko KAYASUGA Nobuyuki SHIMOHATA Hiroyuki KAMATA Shigeki SUZUKI Ryosuke ECHIGO Manabu MOCHIZUKI Ung-Il CHUNG Nobuo SASAKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.11, pp.1511-1516, 2012 (Released:2012-12-01)

参考文献数

41

被引用文献数

2 2

---

Trehalose has several novel anti-inflammatory and cell-protective functions. We hypothesized that lyophilized aspirin/trehalose could decrease the severity of aspirin-induced gastropathy. Thirteen dogs were assigned into aspirin, lyophilized aspirin/trehalose, and control groups, and the gastric lesions were assessed on gastroscopy with the modified Lanza scale. Another 6 dogs were used to measure the plasma aspirin concentration by high-performance liquid chromatography after aspirin or lyophilized aspirin/trehalose administration. The results indicated that lyophilized aspirin/trehalose induced less gastric ulceration than aspirin despite maintaining therapeutic concentrations of plasma aspirin in both the groups. Lyophilized aspirin/trehalose might be a solution to decrease aspirin-induced gastropathy.

著者

Hideharu OCHIAI Ken ONDA Kikumi OGIHARA Yuko NAYA Hiroki SUGIYAMA Takuya MARUO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.11, pp.1505-1510, 2012 (Released:2012-12-01)

参考文献数

24

被引用文献数

1 7

---

A full-length cDNA sequence of canine Na-dependent neutral amino acid transporter (ASCT2) and its distribution were determined. The sequence was 2,090 bp long and was predicted to encode 544 amino acid polypeptides. The amino acid sequence deduced from canine ASCT2 showed 90% similarity to that of humans and mice. Northern blot analysis revealed ASCT2 expression in the kidney, heart, lung and muscles, and Western blot analysis using anti-human ASCT2 antiserum detected the bands at 60 and 65 kDa in membrane protein of the lung. RT-PCR analysis revealed ASCT2 expression in all the tissues examined.

著者

Michelle B. CHRISTENSEN Rebecca LANGHORN Amelia GODDARD Eva B. ANDREASEN Elena MOLDAL Asta TVARIJONAVICIUTE Jolle KIRPENTEIJN Sabrina JAKOBSEN Frida PERSSON Mads KJELGAARD-HANSEN

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0404, (Released:2012-11-28)

被引用文献数

4 8

---

Canine serum amyloid A (SAA) is a useful diagnostic marker of systemic inflammation. A latex agglutination turbidimetric immunoassay (LAT) was validated for automated measurements. The aim of the study was to evaluate the clinical applicability of SAA measured by the LAT. SAA was measured in 7 groups of dogs with and without systemic inflammation (n=247). Overlap performance was investigated. Diagnostic performance was compared to body temperature and leukocyte markers. Clinical decision limits for SAA were estimated. In dogs with neurological, neoplastic, or gastrointestinal disorders (n=143), it was investigated whether a higher proportion of SAA positive dogs could be detected in cases of complications with risk of systemic inflammation. Significantly higher concentrations of SAA were measured in dogs with (range [48.75; 5,032 mg/l]), compared to dogs without systemic inflammation [0; 56.4 mg/l]. SAA was a more sensitive and specific marker of systemic inflammation [area under the receiver-operating characteristic curve (AUC) 1.00], compared to body temperature (0.6) and segmented neutrophils (best performing leukocyte marker, 0.84). A clinical decision limit of 56.4 mg/l was established giving close to perfect discrimination between dogs with and without systemic inflammation. Higher proportions of SAA-positive dogs were observed in dogs with neurological, neoplastic, and gastrointestinal disorders with complications known to increase risk of systemic inflammation, compared to uncomplicated cases. The automated LAT makes SAA applicable as a relevant diagnostic marker of systemic inflammation in dogs for routine random-assess real-time use in a general clinical setting.

著者

Kazuya MATSUDA Kanako SAKAGUCHI Shintaro KOBAYASHI Makiko TOMINAGA Kazuko HIRAYAMA Tsuyoshi KADOSAWA Hiroyuki TANIYAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.71, no.2, pp.229-232, 2009 (Released:2009-03-05)

参考文献数

18

被引用文献数

3 11 3

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A 5-year-old female miniature dachshund presenting with persistent vomiting and diarrhea had two concurrent rare pathological conditions: systemic candidiasis and mesenteric mast cell tumor with multiorgan metastases. Neoplastic mast cells formed mass in the mesentery of the cecal-colonic region and were also found in the liver, spleen, kidneys, lungs, adrenal grands, ovaries, bone marrow and other tissues. The cells had intracytoplasmic granules with metachromasia and were immunohistochemically positive for c-kit and histamine. Granulomatous lesions with fungal organisms were present in the heart, lungs, kidneys, pancreas, subserosal and surrounding adipose tissue of the duodenum, thyroid glands and mesenteric mass, and phagocytosed organisms were detected in the liver and bone marrow. Bacteriologically and immunohistochemically, the fungi were consistent with Candida albicans.

著者

Yasushi HARA Takahiro TESHIMA Takahiro TAODA Hirokazu ISHINO Yoshinori NEZU Yasuji HARADA Takuya YOGO Hiroyuki MASUDA Akira TERAMOTO Masahiro TAGAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.72, no.4, pp.397-404, 2010 (Released:2010-05-03)

参考文献数

53

被引用文献数

4 13

---

Postoperative changes in endocrinological status and serum chemistry during the 4 years after transsphenoidal surgery (TSS) in 25 dogs with Cushing's disease were investigated in a prospective study. In all 25 dogs, Cushing's disease was diagnosed from resected pituitary tissues as a corticotroph adenoma in the anterior lobe of the pituitary. Prior to TSS, all 25 dogs showed hypercortisolemia. After TSS, the ACTH stimulation test showed continued low serum cortisol concentrations in 21 dogs (84%). In addition, the serum thyroid stimulating hormone concentrations decreased sequentially, while the serum T4 concentrations tended to increase due to the postoperative hormone substitution therapy utilized to avoid secondary hypothyroidism. In regard to serum chemistry, alkaline phosphatase (ALP), alanine aminotransferase (ALT) and total cholesterol are commonly increased in canine Cushing's disease. In this study, ALP, ALT and total cholesterol were increased in 23 cases (92%), 19 cases (76%) and 20 cases (80%), respectively. However, postoperatively, these concentrations gradually decreased. The postoperative serum concentrations of ALP at 1 year, that of ALT at six months to 2 years and that of total cholesterol over the course of the 4 years decreased significantly compared with the concentrations before TSS. These results show that TSS is an effective treatment for canine Cushing's disease and for long-term improvement of hypercortisolemia. Moreover, TSS is effective in improvement of hypercortisolism, such as increased concentrations of serum ALP, ALT and total cholesterol.

著者

Takahiro TESHIMA Yasushi HARA Kae SHIGIHARA Susumu TAKEKOSHI Yoshinori NEZU Yasuji HARADA Takuya YOGO Akira TERAMOTO Robert Y. OSAMURA Masahiro TAGAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.71, no.1, pp.93-98, 2009 (Released:2009-02-05)

参考文献数

36

被引用文献数

5 8 1

---

Pituitary thyrotroph hyperplasia results from prolonged primary hypothyroidism in humans, mice and rats. In dogs with Cushing's disease, many cases have low serum thyroid hormones concentrations due to euthyroid sick syndrome. A 6-year-old castrated male Beagle diagnosed with Cushing's disease had a high serum thyroid stimulating hormone (TSH) concentration that was treated by hypophysectomy. On histological examination, the resected pituitary gland contained both a corticotroph adenoma and thyrotroph hyperplasia. The TSH-positive cell ratio in this case was greater than that of healthy Beagles. In the present case, the pituitary thyrotroph hyperplasia was probably caused by primary hypothyroidism. In conclusion, this Beagle is the first histological confirmation of the coexistence of a corticotroph adenoma and thyrotroph hyperplasia.