著者

山森 邦夫 松居 隆 河原 栄二郎 天野 勝文

出版者

北里大学

雑誌

基盤研究(B)

巻号頁・発行日

1997

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タイ国ではカブトガニ類の卵巣を食べる習慣があり、これに伴う食中毒死事件がたまに起こる。原因毒はフグ毒(TTX)や麻痺性貝毒(PSP)などの麻痺性毒である。カブトガニ類は原始的な節足動物であり、世界には3属4種が現存している。アメリカカブトガニ、カブトガニ、ミナミカブトガニ、マルオカブトガニである。タイには後2者が生息しているが、食中毒はもっぱらマルオカブトガニによるものであり、ミナミカブトガニによる中毒例はない。そこで本研究ではマルオカブトガニの毒化機構を明らかにする研究の一部として、カブトガニ類のTTXおよびサキシトキシン(STX)に対する抵抗性を調べ、比較検討した。TTX投与時の最小致死量は、ミナミカブトガニ成体では60〜150MU/20g 体重、アメリカカブトガニ幼体では50〜100MU/20g体重のかなり高い抵抗性を示したが、マルオカブトガニ成体では約900MU/20g体重、幼体では3600MU/20g体重以上となり、前2者を大きく上回った。一方、STXに対する抵抗性はマルオカブトガニ幼体およびアメリカカブトガニ幼体のいずれにおいても100〜200MU/20g体重とかなり高いが、差はなかった。TTX結合タンパク質がクサフグの血漿からDEAEセルロース処理、硫酸アンモニウム分画、Sephad exゲル濾過,Sephacryl S-200とCellulofine A-500によるカラムクロマトグラフィーを経て精製された。TSK G-3000SLカラムを用いた高速液体クロマトグラフィーによる最終精製物は単一のタンパク質ピークを示した。そのタンパク質の分子量はSDS-PAGEおよびmass spectrometryで、それぞれ、116,000および96,000と推定された。精製されたタンパク質のアミノ末端側アミノ酸配列はAla-Pro-Ser-Pro-?-?-?-His-?-Leu-The-Lys-Pro-Val-と推定された。

著者

松居 隆 大塚 幸 酒井 浄

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.120, no.10, pp.825-837, 2000-10-01

被引用文献数

1

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One century has passed since fugu toxin was named tetrodotoxin (TTX) by Tahara. Chemical problems such as crystallization of tetrodotoxin and subsequent structure determination were solved by research groups headed by Tsuda, Hirata, Woodward, and Mosher. The International Symposium on the Chemistry of Natural Products in Kyoto (1964) was well known as symposium which the structure of TTX was internationally clarified. Since the first isolation of toxin from taricha torosa (imori) as natural source except for fugu fishes, distribution of toxin in nature has been widely investigated. And, it was proved that toxin is not produced by fugu fishes, but rather is formed by sea bacteria (30 sp.) such as Alteromonas sp, Vibrio sp, Shewanella. However, it seems to be difficult to explain the tetrodotoxin accumulation at high concentration in fugu by only toxin production by bacteria. TTX analogues were isolated from natural origins such as crabs, fish, annelids, and algae. Based on the structure of these toxin analogues, the biosynthesis of toxin and the structure-activity relationship (Na^+ channel) were proposed by Yasumoto-Yamashita. The findings of wide distribution of toxin in nature may be attributed to development of highly sensitive detection method for toxin. The interesting proposal for the biosynthesis and the structure activity, and the detection method for toxin are outlined in this review.

著者

松居 隆 大塚 幸 酒井 浄

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.120, no.10, pp.825-837, 2000-10-01 (Released:2008-05-30)

参考文献数

36

被引用文献数

4 11

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One century has passed since fugu toxin was named tetrodotoxin (TTX) by Tahara. Chemical problems such as crystallization of tetrodotoxin and subsequent structure determination were solved by research groups headed by Tsuda, Hirata, Woodward, and Mosher. The International Symposium on the Chemistry of Natural Products in Kyoto (1964) was well known as symposium which the structure of TTX was internationally clarified. Since the first isolation of toxin from taricha torosa (imori) as natural source except for fugu fishes, distribution of toxin in nature has been widely investigated. And, it was proved that toxin is not produced by fugu fishes, but rather is formed by sea bacteria (30 sp.) such as Alteromonas sp, Vibrio sp, Shewanella. However, it seems to be difficult to explain the tetrodotoxin accumulation at high concentration in fugu by only toxin production by bacteria. TTX analogues were isolated from natural origins such as crabs, fish, annelids, and algae. Based on the structure of these toxin analogues, the biosynthesis of toxin and the structure-activity relationship (Na+ channel) were proposed by Yasumoto-Yamashita. The findings of wide distribution of toxin in nature may be attributed to development of highly sensitive detection method for toxin. The interesting proposal for the biosynthesis and the structure activity, and the detection method for toxin are outlined in this review.

著者

松居 隆

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.20, no.3, pp.219-221, 1984-03-01

著者

山森 邦夫 出口 進 前原 栄世 松居 隆

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.58, no.6, pp.1157-1162, 1992 (Released:2008-02-29)

参考文献数

17

被引用文献数

8 14

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Lethal effects of tetrodotoxin (TTX) and saxitoxin (STX) on 6 species of nontoxic marine crabs, as well as effects of the toxins on their nerves, were investigated. There was wide variance among species examined for the lethal effect of TTX and STX. The tolerances of nerves are almost compatible to lethal tolerance with the exception of Hemigrapsus sanguineus. This species showed relatively high resistance to TTX, but its nerve was at almost the same level of tolerance as those of other TTX-sensitive crabs. In order to elucidate the mechanism for high TTX-resis-tance in H. sanguineus, nerve tolerance was measured in the presence of its body fluid. The body fluid reduced the effect of TTX on the nerves, while no effect was observed for STX. It is also con-firmed that pre-injection of the body fluid of H. sanguineus into a mouse was effective to some extent in protecting the mouse from TTX attack.