著者

Kouji WADA Hitoshi SASAKI Mitsuya SHIMODA Yutaka OSAJIMA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.51, no.7, pp.1753-1760, 1987 (Released:2006-04-05)

参考文献数

17

被引用文献数

4

---

The aroma profiles of 39 coffee samples (32 arabica and 7 robusta coffees) were evaluated objectively by the coupling of gas chromatographic analysis and two kinds of multivariate analysis (principal component analysis and cluster analysis) and compared with the classification on the basis of a cup test of brewed coffee by cup testers. Robusta coffees were separated from arabica coffees by principal component analysis (PCA) and cluster analysis. Using PCA of only 32 arabica coffee samples, their aroma profiles could be characterized on the first and second principal components. The components responsible for grassy aroma and earthy odor, respectively, were clarified from the factor loadings obtained by PCA. Furthermore, 32 arabica coffees were divided into seven clusters by cluster analysis of the first and second principal components obtained by PCA. Consequently, 39 coffee samples were classified into eight groups and the result was consistent with the classification on the basis of sensory evaluation except for two samples.

著者

Kazuhiko KONDO Masaru OGURA Yuichiro MIDORIKAWA Mitsugi KOZAWA Hiroshi TSUJIBO Yoshihiko INMORI

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.52, no.10, pp.2673-2674, 1988 (Released:2006-04-05)

参考文献数

19

被引用文献数

4

著者

Mitsuaki MORIGUCH

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.46, no.4, pp.955-961, 1982 (Released:2006-03-27)

参考文献数

9

被引用文献数

10

---

Fermentative production of pyruvic acid by yeasts was studied using extracts from citrus natsudaidai peel as a carbon source. Many yeasts showed good growth. Of these yeasts, Debaryomyces coudertii IFO 1381 produced pyruvic acid at high yield. Pretreatment of the peel extract with Amberlite IR-120B (Na+) led to increased production of pyruvic acid. Under optimum conditions, the accumulation of pyruvic acid reached a maximum of 970mg/100ml at 48hr-fermentation. The pyruvic acid from the fermentation broth was identified with lactic acid dehydrogenase and by comparisons of properties of its 2, 4-dinitrophenylhydrazone with those of authentic pyruvic acid in paper chromatography, IR spectrometry and elemental analysis.

著者

SHAH F. H. SEDI M. H. SHEIKH T. H.

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.31, no.6, pp.645-650, 1967

被引用文献数

5

---

Isolation of microorganisms capable of synthesising amino acids, utilizing hydrocarbons, has been reported. These microorganisms were isolated from soil samples by selective culture techniques. 91 strains were found capable of producing amino acids in the broth. Different amino acids and their maximum yield obtained were glutamic acid 160mg/l; leucine 90.0mg/l; isoleucine 40.0mg/1; valine 105.0mg/l; methionine 25.0mg/l; tryptophan 2.5 mg/l; arginine 70.0mg/l; and histidine 10.0mg/l.

著者

Masahiro NISHII Yasuko FUJIHARA Jin-ichiro INAGAKI Kazuo OGAWA Fujio NOHARA Makoto UBUKATA Kiyoshi ISONO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.50, no.10, pp.2697-2699, 1986 (Released:2006-04-05)

参考文献数

5

被引用文献数

1

著者

Hiroshi SASAKI Tomoyoshi HOSOKAWA Yoshiharu NAWATA Kunio ANDO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.38, no.8, pp.1463-1466, 1974 (Released:2008-11-27)

参考文献数

11

被引用文献数

6

---

Ascochlorin and its analogs were isolated from the filter cake of the fermented broth of a fungus, Ascochyta viciae Libert; the compounds obtained were ascochlorin, LL-Z1272δ, LL-Z1272 ε, ascofuranone, ascofuranol and a new analog, C23H31ClO5. Some of these prenyl phenols show hypolipidemic activity in both normolipidemic and hyperlipidemic rats. Details of the structure determination for ascochlorin are presented. The structure of the new analog was elucidated as 4'-hydroxy-5'-hydroascochlorin.

著者

Takashi KIMURA Masahiro YOSHIDA Kayoko OISHI Masafumi OGATA Teruo NAKAKUKI

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.53, no.7, pp.1843-1848, 1989 (Released:2006-04-05)

参考文献数

17

被引用文献数

2 9

---

A dual enzyme system of exo-maltotetraohydrolase [EC 3.2.1.60] and pullulanase [EC 3.2.1.41] was studied for the continuous production of maltotetraose. Porous chitosan beads were selected from among many carriers as the best carrier to immobilize both enzymes. The properties of the immobilized enzymes were examined and compared with those of the native enzymes. For exo-maltotetraohydrolase, the optimum pH of the immobilized enzyme shifted slightly to the acidic side and the pH stability was improved on the alkaline side. The optimum temperature of the immobilized enzyme increased by about 15°C and thermostability was improved by about 10°C. As for pullulanase, very little difference in thermostability was observed. The effects of operating conditions on the continuous production of maltotetraose using exo-maltotetraohydrolase immobilized on the porous chitosan beads were examined. Porous chitosan beads were recognized to be superior to Diaion HP-50. The continuous production of maltotetraose was accomplished using the dual immobilized enzyme system. The dual enzyme system proved to be effective to increase the maltotetraose content in the product. A stable operation was successfully continued for more than 60 days.

著者

Kozo MACHIYA Itsuo ICHIMOTO Kennosuke TONARI Mitsunori KIRIHATA Hiroo UEDA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.49, no.6, pp.1767-1773, 1985 (Released:2006-03-27)

参考文献数

12

被引用文献数

3

---

A convenient synthetic method for (±)-malyngolide (1), an antibiotic from the marine blue-green alga Lyngbya majuscula GOMONT, is described. The compound (±)-1 was synthesized from 2-methylglutaric anhydride (starting material) in four steps, involving the TBHP-based osmiumcatalyzed procedure for vicinal dihydroxylation of the methylene unit in unsaturated carboxylic acid as the key step. The optical resolution of (±)-1 was accomplished by HPLC, in which diastereomeric (+)-2-(4-chlorophenyl)isovaleric acid [(+)-CPIA] esters of (±)-1 derived from (+)-CPIA-C1 could be easily separated, and subsequent hydrolysis of each ester gave the enantiomerically pure (-)-1 and (+)-1, respectively. A synthetic method for α-methylene malyngolide, which is to be expected for biological activity, is also described.

著者

Akira TANAKA Kyohei YAMASHITA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.42, no.8, pp.1585-1588, 1978 (Released:2008-11-27)

参考文献数

24

被引用文献数

4

---

α-Methylene γ- and δ-lactones can be prepared in fair yields by a two step procedure involving formation of the a-hydroxymethylene or α-ethoxyoxalyl sodio derivatives of γ- or δ-lactones followed by their condensation with formaldehyde.

著者

Itsuo ICHIMOTO Kozo MACHIYA Mitsunori KIRIHATA Hiroo UEDA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.54, no.3, pp.657-662, 1990 (Released:2006-04-05)

参考文献数

15

被引用文献数

10

---

A convenient synthetic method for the marine antibiotic (-)-malyngolide and its Stereoisomers was accomplished from a chiral α-alkoxyketone (4), which was readily available as a chiron. Chiral quaternary carbon synthons (5a) and (5b) as the key intermediates were constructed by the chelation controlled addition of Grignard reagent to 4. The diastereomeric mixture of 5a and 5b was readily transformed into a separable mixture of lactones (7a) and (7b), each of which could be easily separated by silica-gel column chromatography. (-)-Malyngolide and its three steroisomers were obtained in optically pure form without the need for optical resolution.

著者

Yoshio YAMASAKI Kazuyuki MAEKAWA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.42, no.9, pp.1761-1765, 1978 (Released:2008-11-27)

参考文献数

21

被引用文献数

14 47

---

A peptide fraction with a delicious taste was isolated from the gravy of beef meat. By successive application of gel filtration with Sephadex G-25, chromatography on an ion exchange resin (Dowex 50×4), and paper electrophoresis, the peptide was purified. The amino acid sequence of the purified peptide was determined by Edman degradation for N-terminal and Cpase A method for C-terminal sequence. As a result, the primary structure of the peptide was proposed as follows; H-Lys-Gly-Asp-Glu-Glu-Ser-Leu-Ala-OH.

著者

Hiroyuki HORITSU Min Yuh WANG Keiichi KAWAI

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.55, no.1, pp.269-271, 1991 (Released:2006-04-05)

参考文献数

4

被引用文献数

3 11

著者

ONO Tomotada CHOI Myoeng Rak IKEDA Ako ODAGIRI Satoshi

出版者

社団法人日本農芸化学会

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.55, no.9, pp.2291-2297, 1991-09-23

被引用文献数

8 42

---

The protein particles in soymilk were fractionated in size by differential centrifugation. Particles of more than 100 nm in diameter (LSP) constituted 40% of the total protein in raw soymilk, 70% of the protein components being 11S globulin. LSP was not formed in the presence of 2-mercaptoethanol and sodium ascorbate. LSP was decreased by heating, and particles of 100-40 nm in diameter (MSP) were increased. The formation of MSP was not due to any degradation of LSP but to the combination of supernatant proteins of less than 40 nm in diameter with each other. MSP formed by heating contained the βsubunit of 7S and the basic subunit of 11S as main components. The particles of more than 40 nm in diameter (LSP+MSP) constituted 50% of the total protein in both raw soymilk and soymilk.

著者

OHMOMO Sadahiro AOSHIMA Ikuko TOZAWA Yukiko UEDA Kiyomoto

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.49, no.9, pp.2767-2768, 1985

被引用文献数

2 1

著者

OHMOMO Sadahiro ITOH Noboru WATANABE Yasuo KANEKO Yasuyuki TOZAWA Yukiko UEDA Kiyomoto

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.49, no.9, pp.2551-2555, 1985

被引用文献数

3 16

---

Continuous decolorization of molasses waste water by mycelia of <i>Coriolus versicolor</i> Ps4a was studied using waste water from a baker's yeast factory, treated by means of methane fermentation and with activated sludge. Optimum decolorization with bare pellet-type mycelia in shaking flasks needed the addition of glucose (0.5%) and peptone (0.05%) and aerobic conditions (1ppm of dissolved oxygen). Continuous decolorization in a bubbling column reactor showed a decolorization yield of approximately 75% in only 20 hr at a dilution rate (<i>D</i>) of 0.03hr^-1 under the optimum conditions.<br> In order to continue the decolorization for a longer time, mycelia immobilized within Ca-alginate gel were tested in a bubbling column reactor under the optimum conditions. The immobilized mycelia showed an almost constant decolorization yield (65.7%) during continuous decolorization for 16 days at <i>D</i>=0.22hr^-1.

著者

AOSHIMA Ikuko TOZAWA Yukiko OHMOMO Sadahiro UEDA Kiyomoto

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.49, no.7, pp.2041-2045, 1985

被引用文献数

9 29

---

The distribution of molasses pigment (melanoidin) decolorizing activity (MDA) was investigated in various Basidiomycetes. MDA was only found in some genera of the white-rot-fungi group of which <i>Coriolus versicolor</i> Ps4a showed high activity, a decolorization yield of approximately 80% under the optimal conditions. Production of MDA by <i>C. versicolor</i> was almost completely coincident with the growth of mycelia. The main MDA was due to intracellular enzymes and induced by the molasses pigment. The induced enzyme consisted of two types, namely a sugar dependent enzyme and a sugar independent enzyme. The decolorization by <i>C. versicolor</i> was due to the decomposition of the molasses pigment.

著者

OHMOMO Sadahiro AOSHIMA Ikuko TOZAWA Yukiko SAKURADA Noriko UEDA Kiyomoto

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.49, no.7, pp.2047-2053, 1985

被引用文献数

11 19

---

Melanoidin decolorizing enzymes (MDE) were extracted from mycelia of <i>Coriolus versicolor</i> Ps4a and purified by DEAE-Sephadex, DEAE-Sephacel and Sephadex G-200 column chromatographies. MDE of this strain consisted of a main fraction, P-fraction, and a minor fraction, E-fraction, and the P-fraction was composed of at least five enzymes. P-III and P-IV in the P-fraction were picked as typical enzymes of this strain, and their enzymatic properties were investigated. P-III had a molecular weight of 48, 400-50, 000, an optimum pH of 5.5 and an optimum temperature of 30-35°C. P-III required glucose and O₂ for the appearance of the activity, and was inhibited by <i>p</i>-CMB, <i>N</i>-BSI, Ag⁺ and <i>o</i>-phenanthroline.<br> On the other hand, P-IV had a molecular weight of 43, 800-45, 000, an optimum pH of 4.0-4.5 and an optimum temperature of 30-35°C. P-IV could decolorize melanoidin in the absence of glucose and O₂, and was inhibited weakly by Ag⁺, <i>p</i>-CME and <i>N</i>-BSI. P-IV is the enzyme that attacks the melanoidin directly in comparison with P-III which attacks melanoidin indirectly as in the sub-reaction of sugar oxidase.<br> Incidentally, a multiplicative effect between P-III and P-IV for decolorization was observed.

著者

Junji MAGAE Kazuo NAGAI Kunio ANDO Gakuzo TAMURA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.52, no.12, pp.3143-3147, 1988 (Released:2006-04-05)

参考文献数

12

被引用文献数

9 1

---

Mouse myeloid leukemia cells, Ml, were induced to differentiate into phagocytes by treatment with ascofuranone (AF). AF also induced differentiation of human promyelocytic leukemia HL60 cells and human erythroid leukemia K562 cells into granulocytes and erythrocytes, as detected by nitroblue tetrazolium reducing activity and benzidine staining, respectively. The antibiotic enhanced acetate incorporation of K562 cells. The increase was not observed with the cells of HL60 and two human B lymphoma lines, Daudi and Raji. The increase was diminished by the addition of a glycolysis inhibitor, deoxyglucose. Inhibitors of respiration, antimycin and sodium azide, also enhanced acetate incorporation of K562 cells specifically, which was diminished by the addition of deoxyglucose. Furthermore, antimycin induced differentiation of K562 and HL60 cells. These results suggest a possible relationship between cell differentiation and inhibition of respiration.

著者

Kenshiro FUJIMOTO Hiroko OHMURA Takashi KANEDA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.50, no.1, pp.101-108, 1986 (Released:2006-04-05)

参考文献数

34

被引用文献数

2

---

The biological antioxidant activities of bromophenols (5-bromo-3, 4-dihydroxy benzaldehyde and 5-bromo-3, 4-dihydroxy benzylalcohol), protocatechualdehyde and vanillyl octadecanamide were compared with α-tocopherol in vitro and in vivo. All of these antioxidants significantly inhibited the lipid peroxidation in microsomal suspensions induced by ascorbate/Fe(II) or NADPH. By removing the non-binding free antioxidants from the suspensions, the inhibitory activities markedly decreased in antioxidants other than α-tocopherol; however, considerable activity was still observed. The effects of orally administered antioxidants on the symptoms of vitamin E deficiency were examined using rats fed with a vitamin E-free diet and supplemented with oxidized oils. As a result and contrary to the effects in vitro, all of the antioxidants were much less effective in liver. However, some of the vitamin E-deficient symptoms such as organic solvent-soluble fluorescent materials in the heart and lung, and oxidative hemolysis were significantly suppressed by some antioxidants.

著者

SHIOZAKI Shozo SHIMIZU Sakayu YAMADA Hideaki

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Agricultural and Biological Chemistry (ISSN:00021369)

巻号頁・発行日

vol.48, no.9, pp.2293-2300, 1984

被引用文献数

11 40

---

In survey studies on microbial accumulation of S-adenosyl-L-methionine (AdoMet), various yeasts were found to accumulate AdoMet intracellularly to a high concentration when they were grown in medium containing L-methionine. A group of sake yeasts (<i>Saccharomyces sake</i>) exhibited especially high accumulation. Of these yeasts, <i>S. sake</i> Kyokai No. 6 (K-6), which exhibited the highest accumulation, produced 12.6 μmol (5.03 mg) of AdoMet/ml broth. Almost all AdoMet produced was accumulated in cells, extracellular accumulation of AdoMet being very low. The maximum content of AdoMet of cells was 5.31 μmol (205 mg)/g dry cells. This was the highest value that had been reported. Methionine adenosyltransferase (EC 2.5.1.6) activity was significantly higher in this yeast compared to those in other yeasts tested. Ultraviolet photomicrographic studies on <i>S. sake</i> K-6 suggested that AdoMet was gradually accumulated in vacuoles with the passage of cultivation time.