著者

Takumi TAKANO Chiaki AOYAMA Yoshiro TERASAKI Kenichi SUZUKI Aki ANDO Yanting SONG Makoto TSUNODA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.37, no.12, pp.1811-1814, 2021-12-10 (Released:2021-12-10)

参考文献数

15

被引用文献数

1

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Therapeutic oligonucleotides have recently been approved in the United States, the EU, and Japan. Hence, the analysis of oligonucleotides is an important topic in drug development. Liquid chromatographic techniques are commonly used for purity verification and the determination of oligonucleotides. In ion-pair reversed-phase separation, several parameters, such as the pore size of the stationary phase, mobile phase additives, and column temperature, were investigated using three types of oligonucleotides (18, 19, and 20 mer). All of the investigated parameters could influence the separation, and they are expected to be useful for optimizing oligonucleotide separation.

著者

Shunsuke FUJIMURA Takenori ITO Shoujiro OGAWA Takayuki ISHIGE Shoichi NISHIMOTO-KUSUNOSE Tatsuya HIGASHI

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.21P268, (Released:2021-10-01)

被引用文献数

4

著者

Yuto MOGI Yoko KEBUKAWA Kensei KOBAYASHI

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.21P188, (Released:2021-08-13)

被引用文献数

2

著者

矢島 敏司 庄子 卓 巽 広輔

出版者

The Japan Society for Analytical Chemistry

雑誌

分析化学 (ISSN:05251931)

巻号頁・発行日

vol.70, no.9, pp.529-533, 2021-09-05 (Released:2021-11-15)

参考文献数

13

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異なる置換基をもつ8種類の水溶性アズレンについて,ニトロベンゼン|水界面におけるイオン移動ボルタンメトリーを行った.その結果,すべての水溶性アズレンにおいて1価アニオンの可逆な移動波が観測され,それぞれの標準移動電位が決定できた.得られた標準移動電位から溶媒間移行標準ギブズエネルギーを算出し,水溶性アズレンの疎水性の評価を行った.また溶媒間移行標準ギブズエネルギーと薬理活性パラメータ(抗潰瘍係数)との相関について検討したところ,おおむね疎水性の高いものが高い活性を示す一方,最も疎水性の高いものが最も低い活性を示す,という逆の関係も見いだされた.これは,疎水性が高いほど生体膜を通過しやすくなるので活性が上がるが,ある程度以上疎水性が高くなると水への溶解度が落ちる,もしくは生体膜へ蓄積されることにより患部へ運ばれにくくなるためと考えられる.

著者

Yanbei ZHU

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.37, no.12, pp.1653-1654, 2021-12-10 (Released:2021-12-10)

参考文献数

31

被引用文献数

2

著者

Nailong GAO Jianguo CHANG Peng DAI Ziming ZHU Hui YOU

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.37, no.12, pp.1695-1700, 2021-12-10 (Released:2021-12-10)

参考文献数

30

被引用文献数

7

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Alpha-fetoprotein (AFP) is an important disease biomarker, relating to cancers such as hepatocarcinomas and gastric cancer. However, traditional methods are time-consuming, relied on bulky instruments and trained professionals, cannot satisfy the demand for low cost and point-of-care testing (POCT). In this study, a power-free POCT device was developed for the rapid and low-cost detection of AFP via one-sampling. Based on the principle of sandwich immunofluorescence, the chip is capable of automatically accomplishing on-chip mixing, labeling and capturing procedures, which only require that operator add 40 μL sample into the chip one time. The proposed device is capable of sensitively detecting human AFP in FBS with a dynamic range of 10 – 1000 ng/mL and LOD (1.88 ng/mL) within a short time of 3 min. Predictably, our method holds a great potential to be applied in the POC diagnostics of proteins, especially for some regions that are resource-limited.

著者

So TANABE Satohiro ITAGAKI Shuyi SUN Kyohei MATSUI Takamasa KINOSHITA Shigeki NISHII Yojiro YAMAMOTO Yasuhiro SADANAGA Hiroshi SHIIGI

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.37, no.11, pp.1597-1601, 2021-11-10 (Released:2021-11-10)

参考文献数

27

被引用文献数

2

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Enterohemorrhagic Escherichia coli are a dangerous bacterium known to be harmful to the human body, with some infections even resulting in death. Given this danger, food factories are required to perform a quick bacterial test to confirm the absence of this pathogen prior to shipping. We have developed a novel molecular imprinting polymer (MIP) particle that has encapsulated gold nanoparticles (AuNPs) and which can function as both a receptor and an optical signal transmitter in biological systems. This MIP particle is artificially synthesized and can be engineered to specifically recognize and capture antigens on the bacterial cell membrane. In addition, MIP particles containing AuNPs generate strong scattered light signals, and binding of the MIP particles improves the optical intensity of the target bacterial cells. This enables clear visualization under a dark-field microscope and quantification of the target bacteria using the scattering light intensity. Here we describe the successful quantification of Escherichia coli O157 cells in real meat samples using this technology in conjunction with a simple labelling step.

著者

Hideyuki TAKEZAWA Kengo ITADANI Ryosuke OBATA Tomonobu SUGIYAMA Tomohiro UCHIMURA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.37, no.10, pp.1453-1457, 2021-10-10 (Released:2021-10-10)

参考文献数

30

被引用文献数

2

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The creaming behavior of an oil-in-water (O/W) emulsion was quantitatively evaluated via resonance-enhanced multiphoton ionization time-of-flight mass spectrometry. Styrene O/W emulsions were prepared with initial styrene concentrations of 1 and 4 g/L, and the height at the center of the sample was monitored. A peak area of the molecular ion of styrene was set as the signal intensity, for which a time profile was constructed from a series of mass spectra. As a result, the averaged time profiles showed that the signal intensities increased once and then decreased with the onset of creaming. In addition, in order to fit an experimentally obtained time profile, a modified fit function was proposed. Based on the fit results, the ratios of the increases and decreases in signal intensities were different between the two emulsions—higher in the case of an O/W emulsion with a higher initial oil concentration. On the other hand, the duration of the enhancement of the signal intensity with the onset of creaming was independent of the initial oil concentration. The present method offers the possibility to quantitatively evaluate the creaming behavior of an emulsion without pretreatment, and, therefore, would be useful for confirming the stability and quality assurance of emulsions.

著者

Seiji SAKAMOTO Itaru HAMACHI

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.35, no.1, pp.5-27, 2019-01-10 (Released:2019-01-10)

参考文献数

224

被引用文献数

30 75

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Chemical modification of proteins is important for creating a myriad of engineered proteins and for elucidating the function and dynamics of proteins in live cells. A wide variety of chemical protein modification methods have been developed and can be categorized into three classes: (i) modification of proteins using the reactivity of naturally occurring amino acids; (ii) modification by bioorthogonal reactions using unnatural amino acids, most of which can be site-selectively incorporated into proteins-of-interest using genetic codon expansion techniques; and (iii) recognition driven chemical modification, which is the only approach that allows modification of endogenous proteins without any genetic manipulation even under heavily crowded and multi-molecular conditions, as in live cells and organisms. All of these approaches have merits and limitations. In this review, we summarize these approaches and discuss their characteristics with respect to specificity, reaction rate and versatility.

著者

Trisna K. SARI Fumiki TAKAHASHI Jiye JIN Rahmiana ZEIN Edison MUNAF

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.34, no.2, pp.155-160, 2018-02-10 (Released:2018-02-10)

参考文献数

29

被引用文献数

27 45

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In this study, nanocomposites of ligand-free gold nanoparticles that are anchored onto the graphene surface (Graphene/AuNPs) were synthesized by a sonochemical method in a single reaction step. A highly sensitive amperometric sensor using Graphene/AuNPs is proposed for the determination of trace hexavalent chromium Cr(VI) in environmental water samples. Compared with a gold electrode, a glassy carbon electrode and a AuNPs modified glassy carbon electrode, the Graphene/AuNPs modified glassy carbon electrode exhibits the highest electrocatalytic activity and stability towards the reduction of Cr(VI), based on the results by cyclic voltammetry and electrochemical AC impedance studies. This study shows that the Graphene/AuNPs-based sensor can detect Cr(VI) with a low detection limit of 10 nM (∼0.5 μg/L), a wide dynamic range of 0 to 20 μM (R = 0.999) and very good selectivity and reproducibility. The electrode is applied to the determination of Cr(VI) in river samples with satisfactory recovery values.

著者

Yuhui MEN Xiaomin ZHOU Zhijie YAN Linqiang NIU Yang LUO Jiamin WANG Jianhong WANG

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.36, no.9, pp.1053-1057, 2020-09-10 (Released:2020-09-10)

参考文献数

51

被引用文献数

6

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A near-infrared (NIR) and water-soluble probe was synthesized and studied for the detection of Cys/Hcy in aqueous solution, living cells and mice. The probe was composed of cyanine derivative as the NIR fluorescent reporting unit and pyrimidiny-thioether moiety as the Cys/Hcy responsive unit. Treatment with Cys/Hcy induced the formation of sulfur-substituted products, then intramolecular rearrangement reaction would occur to produce amino-substituted products and resulting in enhanced red fluorescence emissions. It could be applied to sense Cys/Hcy both in solution with the detection limit of 0.17 μM (or 0.32 μM) and in living cells. Cell imaging experiments proved that such a probe exhibited good cell penetration. In addition, the probe could detect Cys/Hcy in live mice with strong turn-on fluorescent response.

著者

上野 幸三 太刀川 哲平

出版者

The Japan Society for Analytical Chemistry

雑誌

分析化学 (ISSN:05251931)

巻号頁・発行日

vol.11, no.5, pp.554-558, 1962

被引用文献数

1

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亜硝酸ナトリウムによりスルファニルアミドを1~6<I>N</I>塩酸酸性溶液中において7組合わせの双金属電極,白金-アンチモン,白金-ニッケル,白金-タングステン,白金-パラジウム,白金-タンタル,白金-モリブデン,白金-銀を用いて電位差滴定を行ない,おのおのの組合わせの滴定曲線および終点における電位変化を求め,さらに,塩酸の濃度のそれらにおよぼす影響につき比較検討した.白金-タングステンおよび白金-パラジウムの組合わせはピーク形滴定曲線を示し,終点において電位は急激に降下した.ほかの5組合わせはS字形を示し,終点において電位は上昇した.1<I>N</I>塩酸酸性溶液中においては終点の急激な電位変化は求められず,塩酸濃度が高くなるにつれて終点における電位変化の大きさ(Δ<I>E</I>/ΔV)は大きくなった.7組合わせを用いて,6<I>N</I>塩酸酸性溶液中において40~170mg/55m<I>l</I>のスルファニルアミドを定量することができる.<BR>白金-タングステンおよび白金-パラジウムの組合わせはピーク形の滴定曲線を示し,終点において急激に電位降下し,一方,ほかの5組合わせはほぼS字形の曲線を示し,終点において急激に電位上昇した.また,7組合わせのうち,白金-モリブデンが終点における電位変化の大きさは小さく,ほかの組合わせはほぼ同じ大きさであった.1<I>N</I>塩酸酸性溶液の場合は明確な終点が求められず,また,塩酸濃度の増加にともない終点における電位変化の大きさは大きくなった.したがって,その濃度は6<I>N</I>がよい.7組合わせを用いた電位差滴定法によりスルファニルアミド40~170mg/55m<I>l</I>を直接定量することができる.さらに,ほかの芳香族第一級アミンたとえばアニワン,<I>p</I>-ニトロアニリン,<I>α</I>-ナフチルアミンなども本法により定量可能と考えられる.

著者

Jingli QIU Renping XIONG Xuqin SONG Meiyu ZHANG Kanlin PENG Rong LIU Limin HE

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.21P037, (Released:2021-05-21)

被引用文献数

2

著者

Hitomi NAKANO Shintaro KOMATANI Tsugufumi MATSUYAMA Kouichi TSUJI

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.21P111, (Released:2021-05-21)

被引用文献数

3

著者

Toshiro WATANABE Akira YAMAMOTO Shiro NAGAI Shigeru TERABE

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.13, no.4, pp.571-575, 1997-08-10 (Released:2006-07-07)

参考文献数

13

被引用文献数

17 20

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Capillary electrophoretic methods have been developed for the analysis of Monascus yellow pigments for food additives. Monascus yellow pigments were successfully separated by micellar electrokinetic chromatography (MEKC) with a 20mM sodium dodecyl sulfate (SDS) solution containing 50mM phosphate buffer, pH 7.0, and 20% acetonitorile or a 2.0% butyl acrylate/butyl methacrylate/methacrylic acid copolymer sodium salts (BBMA) solution containing 20mM ammonium formate buffer, pH 7.0, and 10% methanol. The xanthomonasin A was identified with the molecular mass measured by FABMS. Monascus yellow pigments were extracted from candies with solid phase extraction and analyzed by the developed technique.

著者

Mehdi KARIMI Alireza BADIEI Ghodsi MOHAMMADI ZIARANI

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.32, no.5, pp.511-516, 2016-05-10 (Released:2016-05-10)

参考文献数

34

被引用文献数

4 10

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A novel organic-inorganic hybrid optical sensor (NUS) was designed and prepared in two steps: the grafting of 3-(isocyanatopropyl)trimethoxysilane onto the surface of SBA-15, and then the attachment of naphthalene-1-amine. The obtained materials were characterized using low-angle XRD, N2 adsorption-desorption, TGA, FT-IR, and TEM techniques. A fluorescence study revealed that the NUS was a highly selective optical sensor for the detection of Cr2O72− among various anions, including F−, I−, Cl−, Br−, CO32−, HCO3−, NO3−, H2PO4−, CH3COO−, and NO2− with a good linearity between the fluorescence intensity and the concentration of Cr2O72− as well as a detection limit of 1.2 × 10−7 M in a 100% aqueous medium. Moreover, the applicability of real samples of the sensor is also discussed.

著者

Shin-Woong KIM Akiko YUMOTO Noriko MINAGAWA Kon SON Yun HEO Yoshihiro ITO Takanori UZAWA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.20SCP20, (Released:2021-01-22)

著者

Yutaro MAEKAWA Eri AYANO Kenichi NAGASE Hideko KANAZAWA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.20SCR09, (Released:2021-01-29)

被引用文献数

8

著者

Hao Wu Seong-Yun Kim

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.21C001, (Released:2021-04-09)

被引用文献数

7

著者

Abdellah MUHAMMED Ahmed HUSSEN Mesfin REDI Takashi KANETA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.37, no.4, pp.585-592, 2021-04-10 (Released:2021-04-10)

参考文献数

56

被引用文献数

10

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Microfluidic paper-based analytical devices (μ-PADs) fabricated in Japan were employed for the determination of total chromium (Cr) in water, soil, and lettuce irrigated with wastewater in Ethiopia. The μ-PADs, which were printed by wax printing in Japan, were transported to Ethiopia and prepared for the determination of total Cr by adding appropriate reagents to the pretreatment and detection zones. Soil and lettuce samples were determined by the μ-PADs and a UV-Vis spectrophotometer in Ethiopia. A paired t-test showed that the mean total Cr concentrations determined in the soil and lettuce samples were not significantly different between μ-PADs and UV-Vis spectrophotometric analysis at the 5% level of significance. This implies that the μ-PADs have good accuracy and reliability, and could be employed to monitor Cr in environmental samples. We found that the total Cr concentrations in all soil and lettuce samples were above the permissible limit. Moreover, evaluating Cr contamination level using the geo-accumulation index indicated that the soils were contaminated with Cr moderately to heavily. Thus, the present work successfully demonstrated the potential of remote investigations of pollution in a less-equipped laboratory by transporting the μ-PADs fabricated in another laboratory.