著者

柳 宇 加藤 信介

出版者

日本建築学会

雑誌

日本建築学会環境系論文集 (ISSN:13480685)

巻号頁・発行日

vol.83, no.754, pp.997-1004, 2018 (Released:2018-12-30)

参考文献数

41

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Since 2005, advances in next-generation sequencing (NGS) technologies have revolutionized biological science. One particular application of NGS technologies is to elucidate microbiomes in built environments. We are currently conducting a series of studies on the elucidation and control of mass infection mechanisms based on dynamic measurement of environment microbiomes. The objective of this study is to clarify the dispersion characteristics of oral bacteria in built environment. Bacterial communities from occupants’ hands and oral cavities, doorknobs, desk and keyboard surfaces, and air in a university laboratory were investigated. For each sample, the variable region 4 (V4) of the bacterial 16S ribosomal RNA (rRNA) gene was amplified by polymerase chain reaction (PCR) using the primer set 5’-acactctttccctacacgacgctcttccgatct-GTGCCAGCMGCCGCGGTAA-3’ (1st_515F) and 5’-gtgactggagttcagacgtgtgctcttccgatct-GGACTACHVGGGTWTCTAAT-3’ (1st_806R). The 16S rRNA amplification protocol version 4_13 was used as a pretreatment with the Next-Generation Sequencer (NGS, Illumina MiSeq system v2). DNA quality was checked using the Agilent 2200 TapeStation. All samples that contained the necessary quality and quantity of nucleic acid concentration for analysis were then analyzed. The produced sequence library was mixed. To improve the quality of the mixed sequence library, a refining processing using the AMPureXP PCR purification system (Beckman Coulter, Inc.) was carried out. About data analysis, the leads for all samples obtained in the sequence analysis (lead 1 and lead 2) were unified, and then assemblies of lead 1 and lead 2 were extracted, followed by clustering and representation arrangement. CD-HIT-OTU was used for alignment extraction and representation arrangement. Basic Local Alignment Search Tool (BLAST) search was performed using the DDBJ 16S rRNA database (version 2016 01 12) by querying representation arrangements. For sequencing, alignment (PyNAST) and genealogical tree creation (FastTree) were performed using a phylogenetic system (RDP classifier), and template alignment was accomplished using the QIIME pipeline. Rarefaction analysis and comparison with a bacillus solution were performed using the QIIME pipeline. The main results obtained by this study are shown as follows. 1) Higher rank 6 phyla of 98% of rates of all 18 phyla being detected and occupying to the whole on a bacterial phyla level more than at composition ratio 1% were Firmicutes (44.9%), Proteobacteria (30.2%), Actinobacteria (9.5%), Bacteroidetes (8.4%), Fusobacteria (3.3%) and Cyanobacteria (1.7%). 2) Higher rank 15 genera of 70% of rates of all 149 genera were Streptococcus (28.4%), Haemophilus (9.0%). Prevotella (5.0%), Staphylococcus (4.5%), Neisseria (4.2%), Corynebacterium (3.8%), Pseudomonas (2.8%), Rothia (2.8%), Fusobacterium (2.1%), Enhydrobacter (1.6%), Veillonella (1.4%), Leptotrichia (1.2%), Granulicatella (1.2%), Acinetobacte (1.1%), Porphyromonas (1.0%). 3) On the species level, reads of one or more larger than 3 orders had 33 species from each sampling point, and about 30% of bacteria were pathogenic bacterium or opportunistic infection bacterium. In particular, P. melaninogenica which is a disease germ in a mouth, and R. dentocariosa and R. mucilaginosa which is an opportunistic infection bacillus in a mouth were detected from all parts. 4) By the analysis of species intersection of each group of bacteria, reads larger than 3 orders from all samples were P. melaninogenica and R. aeria and both bacteria are causative organism of respiratory tract infection. That is, the fact that oral cavity bacteria of this human associated were detected from all surfaces and indoor air showed clearly that oral cavity bacteria had dispersed in all parts among indoor environment.

著者

北澤 幸絵 吉野 博 長谷川 兼一 阿部 恵子 池田 耕一 加藤 則子 熊谷 一清 三田村 輝章 柳 宇 浜田 健佑

出版者

一般社団法人日本建築学会

雑誌

学術講演梗概集 (ISSN:18839363)

巻号頁・発行日

vol.2012, pp.1003-1004, 2012-09-12

著者

高野 大地 池田 耕一 東 賢一 鍵 直樹 柳 宇 大澤 元毅 中川 優馬

出版者

一般社団法人日本建築学会

雑誌

学術講演梗概集 (ISSN:18839363)

巻号頁・発行日

vol.2013, pp.853-854, 2013-08-30

著者

吉野 博 長谷川 兼一 岩前 篤 柳 宇 伊藤 一秀 三田村 輝章 野崎 淳夫 池田 耕一 岸 玲子 持田 灯

出版者

東北大学

雑誌

基盤研究(A)

巻号頁・発行日

2008

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本研究は、ダンプビルの原因となる高湿度環境を解決するための最適設計法・住まい方の提案に資する資料を構築することを目的とする。そのために、住宅のダンプビル問題の実態を全国的規模で把握し、居住環境と居住者の健康状態との関連性を統計的に明らかにした。また、室内の湿度変動を安定させる機能をもった様々な多孔質の建材(調湿建材)等の高湿度環境緩和技術の使用効果について、実測やシミュレーションを用いた評価を行った。