著者
和久 大介 穴田 美佳 小川 博 安藤 元一 佐々木 剛 Waku Daisuke Mika Anada Hiroshi Ogawa Motokazu Ando Takeshi Sasaki
巻号頁・発行日
vol.60, no.3, pp.144-150,

ユーラシアカワウソ(Lutra lutra)はユーラシア大陸に広く分布する中型食肉目である。本種には11の亜種がおり,欧州亜種L. l. lutra,東南アジア亜種L. l. barang,中国亜種L. l. chinensis などが国内外の動物園や水族館で飼育されている。欧州の動物園や水族館にはA-line(L. l. lutra)とB-line(L. l. lutraとL. l. barangが交雑した可能性がある系統)という2つのlineが存在し,日本の動物園や水族館にも導入されている。本来は同じ亜種内で繁殖がおこなわれるが,日本では本種の個体数が少ないためA-lineとB-lineで繁殖がおこなわれている。実際にB-lineが東南アジア亜種の遺伝子を持っているか評価がおこなわれ,B-lineとA-lineの間に違いがあることが示された。ただし先行研究では,解析配列が307bpと短いことが問題として上げられる。本研究ではミトコンドリア(mt)DNA Cytochrome bの全長配列(1140bp)をA-line,B-line各1個体,中国亜種2個体から決定し,先行研究で決定されたB-lineの配列を含む4配列を加えて配列比較,系統解析をおこなった。その結果,A-line,B-lineそれぞれが特徴的な変異サイトを示し,系統解析では先行研究と同じようにB-lineは中国亜種とクレイドを形成し,A-lineは欧州亜種独自のクレイドを形成した。よって解析したB-lineのmtDNAは東南アジア亜種に由来する可能性がある。2015年現在,日本のB-line個体は全て本研究で解析したB-lineの子や孫である。亜種間交雑が示唆された国内のB-lineは,本種の繁殖・維持に活用できるが,A-lineや中国亜種の系統維持に活用することはできない。
著者
Rajib Neupane Xiongjie Jin Takeshi Sasaki Xiang Li Toyoaki Murohara Xian Wu Cheng
出版者
The Japanese Circulation Society
雑誌
Circulation Journal (ISSN:13469843)
巻号頁・発行日
pp.CJ-19-0114, (Released:2019-05-16)
参考文献数
62

Atherosclerotic cardiovascular disease (ACVD) is an inflammatory phenomenon that leads to structural abnormality in the vascular lumen due to the formation of atheroma by the deposition of lipid particles and inflammatory cytokines. There is a close interaction between innate immune cells (neutrophils, monocyte, macrophages, dendritic cells) and adaptive immune cells (T and B lymphocytes) in the initiation and progression of atherosclerosis. According to novel insights into the role of adaptive immunity in atherosclerosis, the activation of CD4+T cells in response to oxidized low-density lipoprotein-antigen initiates the formation and facilitates the propagation of atheroma, whereas CD8+T cells cause the rupture of a developed atheroma by their cytotoxic nature. Peripheral CD4+and CD8+T-cell counts were altered in patients with other cardiovascular risk factors. Furthermore, on evaluation of the feasibility of immune cells as a diagnostic tool, the blood CD4+(helper), CD8+(cytotoxic), and CD4+CD25+Foxp3+(regulatory) T cells and the ratio of CD4 to CD8 cells hold promise as biomarkers of coronary artery disease and their subtypes. T cells also could be a therapeutic target for cardiovascular diseases. The goal of this review was therefore to summarize the available information regarding immune disorders in ACVD with a special focus on the clinical implications of circulating T-cell subsets as biomarkers.
著者
Mihoko Kawabata Masahiko Goya Takeshi Sasaki Shingo Maeda Yasuhiro Shirai Takuro Nishimura Takakatsu Yoshitake Shinya Shiohira Mitsuaki Isobe Kenzo Hirao
出版者
日本循環器学会
雑誌
Circulation Journal (ISSN:13469843)
巻号頁・発行日
pp.CJ-16-1089, (Released:2017-02-07)
参考文献数
22
被引用文献数
9

Background:Atrial fibrillation (AF) is a common cardiac arrhythmia, associated with increased cardiovascular morbidity and mortality including thromboembolic events. The aims of this study were to assess the prevalence of left atrial appendage (LAA) thrombi in Japanese non-valvular atrial fibrillation (NVAF) patients undergoing preprocedural transesophageal echocardiography (TEE) during anticoagulation therapy, and to compare the efficacy of warfarin and direct oral anticoagulants (DOAC).Methods and Results:This retrospective study reviewed records of 559 consecutive NVAF patients (445 men; age, 62±11 years) undergoing preprocedural TEE following at least 3 weeks of anticoagulation therapy. Of these, 275 patients had non-paroxysmal AF (49%). LAA thrombus was observed in 15 patients (2.7%). The prevalence of LAA thrombi was similar between the DOAC group (2.6%) and the warfarin group (2.8%, P=0.86). No patients with CHA2DS2-VASc score=0, or paroxysmal AF without prior stroke or transient ischemic attack, had LAA thrombi. On univariate analysis, non-paroxysmal AF, structural heart disease, antiplatelet therapy, larger left atrium, higher brain natriuretic peptide (BNP), reduced LAA flow, and higher CHA2DS2-VASc score were all associated with LAA thrombi. On multivariate analysis, BNP ≥173 pg/mL remained the only independent predictor of LAA thrombi.Conclusions:LAA thrombi were found in 2.7% of Japanese NVAF patients scheduled for procedures despite ongoing oral anticoagulation therapy. Incidence of thrombi was similar for patients on DOAC and on warfarin.
著者
Takashi YAMAUCHI Toru YOSHIKAWA Takeshi SASAKI Shun MATSUMOTO Masaya TAKAHASHI Machi SUKA Hiroyuki YANAGISAWA
出版者
National Institute of Occupational Safety and Health
雑誌
Industrial Health (ISSN:00198366)
巻号頁・発行日
vol.56, no.1, pp.85-91, 2018-01-31 (Released:2018-02-07)
参考文献数
11
被引用文献数
1 2

In Japan, overwork-related disorders occur among local public employees as well as those in private businesses. However, to date, there are no studies reporting the state of compensation for cerebrovascular/cardiovascular diseases (CCVD) and mental disorders due to overwork or work-related stress among local public employees in Japan over multiple years. This report examined the recent trend of overwork-related CCVD and mental disorders, including the incidence rates of these disorders, among local public employees in Japan from the perspective of compensation for public accidents, using data from the Japanese Government and relevant organizations. Since 2000, compared to CCVD, there has been an overall increase in the number of claims and cases of compensation for mental disorders. Over half of the individuals receiving compensation for mental disorders were either in their 30s or younger. About 47% of cases of mental disorders were compensated due to work-related factors other than long working hours. The incidence rate by job type was highest among "police officials" and "fire department officials" for compensated CCVD and mental disorders cases, respectively. Changes in the trend of overwork-related disorders among local public employees in Japan under a legal foundation should be closely monitored.
著者
Yu Natsume Kasumi Oaku Kentaro Takahashi Wakana Nakamura Ai Oono Satomi Hamada Masahiro Yamazoe Kensuke Ihara Takeshi Sasaki Masahiko Goya Kenzo Hirao Tetsushi Furukawa Tetsuo Sasano
出版者
The Japanese Circulation Society
雑誌
Circulation Journal (ISSN:13469843)
巻号頁・発行日
pp.CJ-17-1194, (Released:2018-02-05)
参考文献数
44
被引用文献数
5

Background:Recent experimental studies have demonstrated that several microRNAs (miRNAs) expressed in atrial tissue promote a substrate of atrial fibrillation (AF). However, because it has not been fully elucidated whether these experimental data contribute to identifying circulating miRNAs as biomarkers for AF, we used a combined analysis of human serum and murine atrial samples with the aim of identifying these biomarkers for predicting AF.Methods and Results:Comprehensive analyses were performed to screen 733 miRNAs in serum from 10 AF patients and 5 controls, and 672 miRNAs in atrial tissue from 6 inducible atrial tachycardia model mice and 3 controls. We selected miRNAs for which expression was detected in both analyses, and their expression levels were changed in the human analyses, the murine analyses, or both. This screening identified 11 candidate miRNAs. Next, we quantified the selected miRNAs using a quantitative RT-PCR in 50 AF and 50 non-AF subjects. The individual assessment revealed that 4 miRNAs (miR-99a-5p, miR-192-5p, miR-214-3p, and miR-342-5p) were significantly upregulated in AF patients. A receiver-operating characteristics curve indicated that miR-214-3p and miR-342-5p had the highest accuracy. The combination of the 4 miRNAs modestly improved the predictive accuracy for AF (76% sensitivity, 80% specificity).Conclusions:Novel circulating miRNAs were upregulated in the serum of AF patients and might be potential biomarkers of AF.