著者

岸本 健雄

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.26, no.4, pp.157-167, 1986-07-25 (Released:2009-05-25)

参考文献数

69

---

Maturation-promoting factor (MPF) is a cytoplasmic factor that is capable of inducing a maturation response involving nuclear envelope breakdown, chromosome condensation and spindle formation when microinjected into immature oocytes. MPF activity is found in a wide variety of eukaryotic cells at M-phase, such as mitotically dividing cells as well as maturing oocytes. MPF acts non-species-specifically among the animal kingdom. MPF has been partially purified and characterized as a heatlabile protein with a molecular size of approx. 5s. MPF activity oscillates with the same period as the cell cycle, with peaks during metaphase. MPF has the ability to amplify itself by activating its precursor, which is stored in fully grown oocytes. But replenishing MPF after its fall during cell cycle requires protein synthesis. MPF can cause nuclear envelope breakdown and chromosome condensation in vitro with isolated nuclei. Such cell-free system requires the cytoplasmic component extracted from eggs in addition to MPF. Further, MPF dissociates the junction between oocyte surface and surrounding follicle cells. Thus, MPF seems to act directly not on nucleus but on cytoplasm. Taken together, MPF might more generally be described as "metaphase-promoting factor" rather than a maturation-promoting factor.

著者

上川内 あづさ 伊藤 啓

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.50, no.6, pp.282-285, 2010 (Released:2010-11-25)

参考文献数

16

---

The human ability to sense gravity and sounds relies on specialized vestibular and auditory organs, respectively, in our inner ear. In the fly, the ability to hear has been ascribed to the antenna, whereas the gravity sensor had remained unidentified. We found that the fly has implemented both sensory modalities into a single system, the Johnston’s organ, which houses specialized clusters of mechanosensory neurons. Each cluster monitors specific movements of the antenna and feeds into distinct neural pathways that are reminiscent of the vestibular and auditory pathways, respectively, in our brain.

著者

谷口 雄一 大野 雅恵

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.59, no.6, pp.305-309, 2019 (Released:2019-11-25)

参考文献数

14

---

The nucleosome arrangement in the genome is an important long-standing problem in biology. To address this, we recently developed a new technology for investigating 3D positions and orientations of nucleosomes across the genome. Analysis of the yeast genome revealed that the nucleosome arrangement is composed of two basic structures, named α-tetrahedron and β-rhombus. Further, we discovered that the nucleosome arrangement is distinct at every genomic locus depending on epigenetic regulation. The results provide the molecular basis of transcriptional and epigenetic events on the genome.

著者

有賀 隆行 富重 道雄 水野 大介

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.59, no.6, pp.300-304, 2019 (Released:2019-11-25)

参考文献数

20

被引用文献数

1

---

Molecular motors are nonequilibrium open systems that convert chemical energy to mechanical work. The nonequilibrium energetics of single molecule kinesin were investigated by measuring the motion of an attached probe particle and its response to external forces with optical tweezers. The sum of the heat dissipation estimated from the violation of the fluctuation-response relation and the output power was inconsistent with the input free energy rate, indicating that large internal dissipation exists. Here we introduce the theoretical basis of the dissipation measurement and our recent experimental results, discussing the physiological implications of the hidden dissipation in the kinesin motor.

著者

今井 清博

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.12, no.3, pp.114-129, 1972-05-25 (Released:2009-05-25)

参考文献数

78

著者

片岡 幹雄

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.52, no.2, pp.070-071, 2012 (Released:2012-03-30)

著者

阿部 奈保子 阿部 洋

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.57, no.1, pp.5-10, 2017

被引用文献数

1

---

<p>In an <i>E. coli</i> cell-free translation system, we found that a circular RNA containing an infinite open reading frame produced more translation product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA. We then tested circular RNAs containing an infinite open reading frame could be translated in eukaryotic systems, in the absence of any particular element for internal ribosome entry, a poly-A tail, or a cap structure. We found that the circular RNAs also produced long peptides in eukaryotic translation systems, possibly owing to the rolling circle amplification mechanism.</p>

著者

石坂 優人 安部 秀哉

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.59, no.5, pp.282-283, 2019 (Released:2019-09-27)

参考文献数

3

被引用文献数

1

著者

金子 邦彦 四方 哲也

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.40, no.supplement, pp.S98, 2000-08-05 (Released:2017-05-01)

著者

今元 泰 針貝 美樹 上久保 裕生 山崎 洋一 片岡 幹雄

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.43, no.supplement, pp.S195, 2003-08-25 (Released:2017-05-01)

著者

神取 秀樹

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.55, no.6, pp.291-298, 2015 (Released:2015-11-28)

参考文献数

30

被引用文献数

1

---

Photoreceptive proteins absorb light by chromophore molecule, and convert light into energy or signal. Ultrafast photoreactions such as electron transfer and isomerization allow efficient transitions from the electronically excited state of the chromophore, and stored light energy is utilized for each function. In case of light-energy conversion, light energy is finally stored as membrane potential, where proton is a principal component. Quinone pool facilitated by proton-coupled electron transfer in photosynthetic reaction center is a smart system to gain proton motive force. In contrast, light-driven ion pumping rhodopsins directly translocate protons, cations and anions. Ion-transporting rhodopsins are key tools in optogenetics, which revolutionize life sciences by control with light.

著者

御手洗 菜美子

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.58, no.3, pp.175-176, 2018 (Released:2018-05-31)

著者

柴田 貴弘 山下 紗季 加藤 薫 太田 善浩

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.56, no.2, pp.112-115, 2016 (Released:2016-03-31)

参考文献数

7

著者

藤原 郁子

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.59, no.4, pp.192-196, 2019 (Released:2019-07-25)

参考文献数

18

---

Latrunculin A (LatA) is the widely used reagent to depolymerize actin filaments. Its mechanism has been thought that LatA sequesters actin monomers from polymerization. Recent observation of single actin filaments by TIRF microscopy found that the binding affinity of LatA to actin monomers depends on the nucleotide status on actin. The observation of actin filaments also showed that LatA binds to actin filaments. LatA increased the depolymerization rate at ends of filaments assembled from ATP-actin to the rates of ADP-actin, but did not change these rates of ADP- or ADP-Pi-bound actin filaments. LatA also severed actin filaments. Thermodynamic analysis proposes that LatA accelerates phosphate release only at ends of actin filaments. Rapid depolymerization, severing and sequestering monomers are mechanisms of LatA to inhibit cellular actin cytoskeleton.

著者

伊藤 秀城

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.59, no.4, pp.230-231, 2019 (Released:2019-07-25)

著者

木村 誠

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.46, no.5, pp.279-282, 2006 (Released:2006-09-25)

参考文献数

15

著者

戸田 聡

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.59, no.3, pp.151-153, 2019 (Released:2019-05-25)

参考文献数

9

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.54, no.3, pp.177-178, 2014

著者

宮川 伸

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.53, no.1, 2013

著者

Ohba Hiroaki Fujii satoshi Sarai Akinori

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.49, 2009