著者
三巻 祥浩 指田 豊 安達 得夫 吉成 清 YOSHIHIRO MIMAKI YUTAKA SASHIDA TOKUO ADACHI KIYOSHI YOSHINARI 東京薬科大学 東京薬科大学 東京薬科大学 東京薬科大学 Tokyo College of Pharmacy Tokyo College of Pharmacy Tokyo College of Pharmacy Tokyo College of Pharmacy
出版者
日本生薬学会
雑誌
生薬學雜誌 = Natural medicines (ISSN:13403443)
巻号頁・発行日
vol.48, no.1, pp.86-91, 1994-03-20

Barks of Prunns lannesiana var. speciosa, P. maximowiczii, P. apetala, P. pendula forma ascendens and P. yedoensis of the subgenus Cerasus, and P. laurocerasus and P. spinulosa of the subgenus Laurocerasus were assayed for their constituents. The barks of the Cerasus plants were formed to be rich in flavonoids and their glucosides, and to contain 5, 4'-dihydroxy-7-methoxyflavanone 5-O-β-D- glucopyranoside (sakuranin) as a common flavonoid. On the other hand, no flavonoid was detected in the barks of the Padus and Laurocerasus plants, but they both contained cyanogenic glucosides as a common component. The difference of the constituents observed between the barks of the Cerasus plants and those of the Padus and Laurocerasus was well correlated to the taxonomic theory proposed by T. T. Yu (1984).
著者
黒田 明平 木下 歩美 椎 崇 社本 典子 三巻 祥浩
出版者
一般社団法人日本医療薬学会
雑誌
医療薬学 (ISSN:1346342X)
巻号頁・発行日
vol.43, no.11, pp.619-629, 2017-11-10 (Released:2018-11-10)
参考文献数
17
被引用文献数
1 1

Pseudohyperaldosteronism and associated hypokalemia are serious adverse effects caused by glycyrrhizinic acid (GA) present in Kampo medicines containing Glycyrrhizae Radix (GR); GA is the major component of GR. To obtain knowledge about the effective and safe use of Kampo medicines, we analyzed the quantities of GA in Kampo medicines by HPLC. The quantities of GA in 13 Kampo decoctions containing GR (2-6 g/day) were found to be almost 2-3.5 fold higher than those of the corresponding Kampo extract products. Among the Kampo extract products containing GR (3 g/day), the GA quantity in Shoseiryuto was significantly lower compared with that in the others, and Pinelliae Tuber as well as Schisandrae Fructus were found to contribute to the decrease in GA quantity. The GA quantity in the Yokukansakachinpihange decoction was found to be lower than that in the Yokukansan decoction; this was caused by the presence of Pinelliae Tuber and Citri Unshiu Pericarpium. Analysis of inter-product variations in GA quantities among the Kampo extract products revealed a maximum 2.6-fold difference in the quantities in the Shoseiryuto extract products from different companies. Our analyses show that GA quantities in Kampo medicines are influenced by the presence of concomitant crude drug constituents. This results in differences in GA quantities between decoctions and extract products having the same Kampo formula, and also among the Kampo extract products from different companies. It is recommended that GA quantities in Kampo extract products should be presented to prevent the adverse effects caused by GA.
著者
三巻 祥浩 神本 敏弘 黒田 明平 指田 豊 西野 敦子 里見 佳子 西野 輔翼
出版者
The Pharmaceutical Society of Japan
雑誌
Chemical and Pharmaceutical Bulletin (ISSN:00092363)
巻号頁・発行日
vol.43, no.7, pp.1190-1196, 1995-07-15 (Released:2008-03-31)
参考文献数
25
被引用文献数
17 27

Phytochemical study on the underground parts of Hosta longipes gave six new steroidal saponins together with a known one. The structures of the new compounds were determined by detailed analysis of their 1H-and 13C-NMR spectra including two-dimensional NMR spectroscopy, acid-catalyzed hydrolysis followed by chemical correlation, and by comparison with spectral data of known compounds. The isolated saponins and their aglycones were examined for inhibitory activity on 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated 32P-incorporation into phospholipids of HeLa cells to identify new antitumor-promoter compounds.
著者
油井 聡 三上 正彰 三巻 祥浩 指田 豊 山崎 正利
出版者
公益社団法人日本薬学会
雑誌
藥學雜誌 (ISSN:00316903)
巻号頁・発行日
vol.121, no.2, pp.167-171, 2001-02-01
被引用文献数
16

We previously demonstrated that Amaryllidaceae alkaloids, lycorine and lycoricidinol, inhibit induction of apoptosis by calprotectin derived from neutrophils, and that the latter alkaloid showed suppression in rat adjuvant-induced arthritis model. These findings suggest that the alkaloids have a modulating activity against inflammatory reaction. To explore further the mechanism of the suppression for inflammation, we studied the effect of the alkaloids on macrophage tumor necrosis factor(TNF-α) production in vitro, since TNF-α is recognized as a pivotal cytokine to regulate inflammation. As a result of this study, lycorine and lycoricidinol inhibited TNF-α production of murine macrophages stimulated with lipopolysaccharide(ID_<50> were 0.2μg/ml and 0.002μg/ml, respectively). The inhibition was also observed in macrophages treated by Gram-positive bacteria, Enterococcus faecalis. Both lycorine and lycoricidinol reportedly have inhibitory activity for protein biosynthesis. Although the inhibition of TNF-α production by lycoricidinol was mainly due to the inhibition of protein biosynthesis, lycorine showed inhibition against TNF-α prodictuon at lower concentrations than the case that they inhibited ^<35>S-Cysteine/^<35>S-Methionine incorporation into macrophages. These facts sugges that the inhibition of TNF-α production is not due to the inhibitory activity against protein translation at least at lower concentrations. From these results, it was concluded that these alkaloids exert inhibitory effects not only on neutrophil apoptosis-inducing protein, calprotectin, but also on macrophage TNF-α production.