1 0 0 0 リボゾームDNA増幅によるティザー菌の検出
- 著者
- 後藤 一雄 伊藤 豊志雄
- 出版者
- Japanese Association for Laboratory Animal Science
- 雑誌
- Experimental Animals (ISSN:00075124)
- 巻号頁・発行日
- vol.43, no.3, pp.389-394, 1994
- 被引用文献数
- 2
ティザー菌をPCR法を用いて, 高感度かつ特異的に検出する目的で, マウス由来ティザー菌 (MSK株) の16SリボゾームDNA (rDNA) の塩基配列を決定し, その配列をRJ株 (ラット由来) の配列と比較した。決定された配列はMSK株およびRJ株で相同性が高かったが (97%以上) , 他の菌種との相同性は低かった (70~83%) ことからティザー菌種に特異的配列を含んでいると考えられた。そこで, PCR法でTyzzer菌種を特異的に検出するプライマーをこれらの塩基配列をもとに選択した。選択されたプライマーを用いたPCR法では, ティザー菌2株 (MSK株およびRJ株) の他, ハムスター由来のHN株を検出できたが, ティザー菌以外の細菌は検出されず, ティザー菌種に対して高い特異性を示した。また, 1個以上のティザー菌 (RJ株) が検出可能であった。さらに, 9匹のJc1: Wistarラットに2×10<SUP>4</SUP>の菌を経口的に実験感染させ, 接種後1, 3および5日目にそれぞれ3匹ずつ肝臓, 心臓, 盲腸, 脾臓および腸間膜リンパ節を採材しPCR法および蛍光抗体法を用いて菌検索を行った。接種後1および3日目の材料からはいずれの方法においても菌は検出されなかった。しかし, 接種後5日目のラットのうち1匹は肝臓および心臓から, 他1匹は盲腸からそれぞれ両方法によって菌が検出された。これらの結果から, 今回明らかにされたMSK株16SrDNAの塩基配列はティザー菌種間でよく保存されていること, さらに選択されたプライマーを用いたPCR法が高感度かつ特異的なティザー菌診断法として有用であることが示唆された。
- 著者
- 伊藤 豊 矢野 真吾 渡辺 和夫 山中 悦二 相見 則郎 坂井 進一郎
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Chemical and Pharmaceutical Bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.38, no.6, pp.1702-1706, 1990-06-25 (Released:2008-03-31)
- 参考文献数
- 16
- 被引用文献数
- 10 18
We investigated the selectivities and structure requirements for alpha-1 and alpha-2 adrenoceptor blocking activities of yohimbine (YO) and its 12 related analogs, such as β-yohimbine (β-YO), dihydrocorynantheine (DHC) and (-)indoloquinolizidine ((-)IQ). The affinity of YO analogs to alpha-adrenoceptor was assessed by measuring their blockade of pressor responses to epinephrine in pithed rats. Among YO structure groups, the potency order was YO>DHC=β-YO>geissoschizine methylether>14β-hydroxy YO>14β-benzoyloxy YO (inactive). (-)IQ was slightly less potent than YO, but much stronger than (+)IQ. Among (±)IQ structure groups, the potency order was (±)IQ>(±)1, 12b-trans-1-hydroxy IQ»(±)1, 12b-cis-1-hydroxy IQ (imactive). (±)Borrerine was active, but (±)desmethylborrerine was inactive. The alpha-1 blocking activities of the four compounds YO, β-YO, DHC and (-)IQ, were assessed in experiments of pressor responses to methoxamine in pithed rats and contractile responses to methoxamine in the rat vas deferens. The potency order was (-)IQ>YO>DHC>β-YO. Furthermore, the alpha-2 blocking activities of the four analogs were assessed in experiments of pressor responses to clonidine and inhibition of electrically driven cardioacceleration by clonidine, in pithed rats. The potency order was YO>β-YO>(-)IQ>DHC. Based on the potency ratio between alpha-1 and alpha-2 blocking activities, DHC or YO was most selective for alpha-1 or alpha-2 subtype, respectively, among the four YO analogs. These results suggest that the A, B, C and D rings of YO analogs and their planarity are necessary for the affinity to alpha-adrenoceptors and that the predominant conformation of the carboxymethyl or hydroxy group on the E ring of YO structure determines the selectivity for alpha-1 and alpha-2 blocking activities.
- 著者
- 伊藤 豊 矢野 真吾 渡辺 和夫 山中 悦二 相見 則郎 坂井 進一郎
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- Chem Pharm Bull (Tokyo) (ISSN:00092363)
- 巻号頁・発行日
- vol.38, pp.1702-1706, 1990
- 被引用文献数
- 2
We investigated the selectivities and structure requirements for alpha-1 and alpha-2 adrenoceptor blocking activities of yohimbine (YO) and its 12 related analogs, such as β-yohimbine (β-YO), dihydrocorynantheine (DHC) and (-)indoloquinolizidine ((-)IQ). The affinity of YO analogs to alpha-adrenoceptor was assessed by measuring their blockade of pressor responses to epinephrine in pithed rats. Among YO structure groups, the potency order was YO>DHC=β-YO>geissoschizine methylether>14β-hydroxy YO>14β-benzoyloxy YO (inactive). (-)IQ was slightly less potent than YO, but much stronger than (+)IQ. Among (±)IQ structure groups, the potency order was (±)IQ>(±)1,12b-trans-1-hydroxy IQ≫(±)1,12b-cis-1-hydroxy IQ (imactive). (±)Borrerine was active, but (±)desmethylborrerine was inactive. The alpha-1 blocking activities of the four compounds YO, β-YO, DHC and (-)IQ, were assessed in experiments of pressor responses to methoxamine in pithed rats and contractile responses to methoxamine in the rat vas deferens. The potency order was (-)IQ>YO>DHC>β-YO. Furthermore, the alpha-2 blocking activities of the four analogs were assessed in experiments of pressor responses to clonidine and inhibition of electrically driven cardioacceleration by clonidine, in pithed rats. The potency order was YO>β-YO>(-)IQ>DHC. Based on the potency ratio between alpha-1 and alpha-2 blocking activities, DHC or YO was most selective for alpha-1 or alpha-2 subtype, respectively, among the four YO analogs. These results suggest that the A, B, C and D rings of YO analogs and their planarity are necessary for the affinity to alpha-adrenoceptors and that the predominant conformation of the carboxymethyl or hydroxy group on the E ring of YO structure determines the selectivity for alpha-1 and alpha-2 blocking activities.
- 著者
- 伊藤 豊 矢野 真吾 渡辺 和夫 山中 悦二 相見 則郎 坂井 進一郎
- 出版者
- 公益社団法人 日本薬学会
- 雑誌
- CHEMICAL & PHARMACEUTICAL BULLETIN (ISSN:00092363)
- 巻号頁・発行日
- vol.38, no.6, pp.1702-1706, 1990
- 被引用文献数
- 18
We investigated the selectivities and structure requirements for alpha-1 and alpha-2 adrenoceptor blocking activities of yohimbine (YO) and its 12 related analogs, such as β-yohimbine (β-YO), dihydrocorynantheine (DHC) and (-)indoloquinolizidine ((-)IQ). The affinity of YO analogs to alpha-adrenoceptor was assessed by measuring their blockade of pressor responses to epinephrine in pithed rats. Among YO structure groups, the potency order was YO>DHC=β-YO>geissoschizine methylether>14β-hydroxy YO>14β-benzoyloxy YO (inactive). (-)IQ was slightly less potent than YO, but much stronger than (+)IQ. Among (±)IQ structure groups, the potency order was (±)IQ>(±)1, 12b-trans-1-hydroxy IQ»(±)1, 12b-cis-1-hydroxy IQ (imactive). (±)Borrerine was active, but (±)desmethylborrerine was inactive. The alpha-1 blocking activities of the four compounds YO, β-YO, DHC and (-)IQ, were assessed in experiments of pressor responses to methoxamine in pithed rats and contractile responses to methoxamine in the rat vas deferens. The potency order was (-)IQ>YO>DHC>β-YO. Furthermore, the alpha-2 blocking activities of the four analogs were assessed in experiments of pressor responses to clonidine and inhibition of electrically driven cardioacceleration by clonidine, in pithed rats. The potency order was YO>β-YO>(-)IQ>DHC. Based on the potency ratio between alpha-1 and alpha-2 blocking activities, DHC or YO was most selective for alpha-1 or alpha-2 subtype, respectively, among the four YO analogs. These results suggest that the A, B, C and D rings of YO analogs and their planarity are necessary for the affinity to alpha-adrenoceptors and that the predominant conformation of the carboxymethyl or hydroxy group on the E ring of YO structure determines the selectivity for alpha-1 and alpha-2 blocking activities.
1 0 0 0 OA 「アメリカン・ボード日本ミッション関連資料」(仮題)の概要について
- 著者
- 伊藤 豊
- 出版者
- 山形大学
- 雑誌
- 山形大学紀要. 人文科学 = Bulletin of Yamagata University. Humanities
- 巻号頁・発行日
- vol.17, no.1, pp.21-37, 2010-02-15
The American Board of Commissioners for Foreign Missions (ABCFM) was one of the largest Protestant missionary organizations active in Meiji Japan. The Japan Mission of the ABCFM gained a considerable following in the Kansai area, thus succeeding in establishing its sphere of influence there. The Japan Mission’s stronghold was the Doshisha School of Theology in Kyoto. The ABCFM missionaries in Kyoto also worked as teachers at other Doshisha schools. Doshisha eventually developed into one of the most prestigious private universities in Japan. Because of this particular background and setting, the present-day Doshisha University has become a major depository of historical papers relating to the Japan Mission. When I attended a research meeting at Doshisha several years ago, I was introduced to bundles of old, unsorted letters and documents that had long been left untouched in a storage cabinet. After only a glance I realized that in all probability they should be regarded as a constituent part of the Doshisha-owned Japan Mission papers. Nevertheless, no other scholars had ever bothered to examine their contents and details until I volunteered to do so. After a painfully time-consuming process of sorting and indexing, I have found that these materials numbering over 3,000 are tremendously informative sources on the history of the Japan Mission. Their most remarkable academic merit is that they include a variety of newly discovered manuscripts. Especially important among them are more than 2,000 pieces of correspondence from the ABCFM missionaries in Japan to Dewitt C. Jencks, who acted as the secretary of the Japan Mission from 1877 to 1887. Now that the indexing is nearly complete, I offer in this article an overview of these long neglected documents, and then demonstrate their historical significance.
- 著者
- 伊藤 豊 加藤 熈 久保木 芳徳
- 出版者
- 特定非営利活動法人日本歯周病学会
- 雑誌
- 日本歯周病学会会誌 (ISSN:03850110)
- 巻号頁・発行日
- vol.36, no.1, pp.56-66, 1994-03-28
- 被引用文献数
- 19 4
本研究は,リコンビナントヒトBMP-2(rhBMP-2)を歯周治療に応用するため,担体としてウシ真皮より抽出しテロペプチドを除去したコラーゲンより作製した強化コラーゲン線維膜(FCM3)を用いた場合の有効性を検討する目的で,12週齢成体ラットの背部皮下と口蓋部に移植実験を行い,病理組織学的に評価した。その結果,移植3週後背部皮下では骨形成はみられなかったが,口蓋部ではrhBMP-2を1.0μgと2.0μg配合した群で骨形成が観察された。またその骨形成の過程は,1週目頃担体の外側部で盛んになり,3週目には母骨と連続し担体周囲を覆い,その後は担体内部へと向かい6週後には母骨とほぼ一体化していた。炎症反応はほとんどみられなかった。以上の結果より,FCM3を担体としてrhBMP-2を歯周治療へ応用できる可能性が高いと考えられた。
1 0 0 0 OA 新規浄水発生土の資源化と鉄・ケイ素供給による環境保全型水稲生産の安定化技術の構築
- 著者
- 伊藤 豊
- 出版者
- エルゼビア・ジャパン
- 雑誌
- クリニカルプラクティス (ISSN:13494252)
- 巻号頁・発行日
- vol.25, no.2, pp.157-160, 2006-02
- 著者
- 松本 敦至 伊藤 豊 齋藤 彰 川浪 雅光 加藤 〓 久保木 芳徳
- 出版者
- 特定非営利活動法人日本歯周病学会
- 雑誌
- 日本歯周病学会会誌 (ISSN:03850110)
- 巻号頁・発行日
- vol.36, no.1, pp.149-161, 1994-03-28
- 被引用文献数
- 14 1
第1報で,Bone morphogenetic protein(BMP)の担体として強化コラーゲン線維膜(FCM)が有効であることを報告した。しかし,FCMは吸湿すると強度が低下し,操作が難しいという欠点があったため,この点を改良した強化コラーゲン線維膜第3号(FCM3)を新たに開発した。まず,FCM3のBMP担体としての有効性について検討する目的で,成体ラットの背部皮下と口蓋部骨膜下に移植した結果,背部皮下で異所性骨形成が,口蓋部骨膜下で母床骨に連続し,隆起した骨形成が観察された。次に,BMP配合FCM3が歯周組織再生に及ぼす影響について検討する目的で,成体ラットの人工的歯槽骨欠損に移植した結果,移植してない群に比べて早期(1週)から骨芽細胞様細胞の増殖が見られ,3週では移植材をとり囲むように骨が新生し,6週では移植していない群に比べて歯槽骨頂が高く,幅も広い傾向が見られた。このことから,BMP配合FCM3は歯周組織の再建に有効である可能性が示唆された。