著者
広津 崇亮
出版者
社団法人 におい・かおり環境協会
雑誌
におい・かおり環境学会誌 (ISSN:13482904)
巻号頁・発行日
vol.46, no.3, pp.191-199, 2015-05-25 (Released:2019-02-20)
参考文献数
48

微量のにおい物質の検知には,従来は物理・化学的手法が用いられてきたが,感度,選択性を追求すると大型化,高コスト化を招く問題があった.そこで近年注目されているのが,生物の嗅覚の高感度性,高選択性を生かしたバイオセンサである.その中には,生物の嗅覚機構を模倣するセンサ,生物の機構や設計思想を活用するセンサが含まれるが,本稿では生物そのものを利用するセンサについて紹介する.最近線虫C. elegansががんのにおいを高精度に識別できることが発見された.線虫嗅覚を用いたがん診断技術(n-nose)は,高感度,低コスト,非侵襲性,簡便,早期がんを発見できるなど,これまでのがん検査システムを変える可能性を秘めている.
著者
魚住 隆行 広津 崇亮
出版者
公益社団法人 日本薬学会
雑誌
YAKUGAKU ZASSHI (ISSN:00316903)
巻号頁・発行日
vol.139, no.5, pp.759-765, 2019-05-01 (Released:2019-05-01)
参考文献数
17
被引用文献数
1

Early detection and treatment are important for the successful eradication of various cancers; therefore, the development of economical, noninvasive novel cancer screening systems is critical. Previous reports using canine scent detection have demonstrated the existence of cancer-specific odors. However, it is difficult to introduce canine scent recognition into clinical practice because of the need to maintain accuracy. In this study, we developed a Nematode-Nose (N-NOSE) test using Caenorhabditis elegans to provide a novel, highly accurate cancer detection system that is economical, painless, rapid, and convenient. We demonstrated that wild-type C. elegans displayed attractive chemotaxis toward human cancer cell secretions, cancer tissues, and urine from cancer patients but avoided control urine. In parallel, C. elegans olfactory neurons showed a significantly stronger response to urine from cancer patients than to control urine. In contrast, G protein α mutants and animals with ablated olfactory neurons were not attracted to urine from cancer patients, suggesting that they sense odors in urine. We tested 242 samples to measure the performance of the N-NOSE test and found that the sensitivity was 95.8%, which is markedly higher than that of other existing tumor markers. Furthermore, the specificity was 95.0%. Importantly, this test could detect various cancer types tested at the early stage (stage 0 or 1). C. elegans scent-based analyses therefore might provide a new strategy for the detection and study of disease-associated scents.