2 0 0 0 OA 植物精油の口腔微生物に対する抗菌性およびバイオフィルム形成抑制効果の検討
- 著者
- 角田 衣理加 大島 朋子 前田 伸子
- 出版者
- 日本歯科薬物療法学会
- 雑誌
- 歯科薬物療法 (ISSN:02881012)
- 巻号頁・発行日
- vol.28, no.1, pp.1-10, 2009-04-01 (Released:2009-12-22)
- 参考文献数
- 22
- 被引用文献数
- 1
Aromatherapy has been widely used in Europe as a phytotherapy for promoting general health or as a beauty treatment, however, the effectiveness of aromatherapy in the field of dentistry has not yet been determined.The purposes of this study were to estimate the anti-microbiological and anti-biofilm forming effects of essential oils against mutans streptococci, periodontal pathogens and oral indigenous true fungi. Seven essential oils, a blended essential oil with tea tree, peppermint, and lemon oils (TPL oils) which is known as a recommended blend for preventing halitosis, and one of the TPL oils replaced by another essential oil were used in this study. All tested essential oils showed anti-microbiological, anti-biofilm forming effects against the tested microorganisms. Specifically, lemongrass and peppermint oils had strong anti-microbiological effects. In addition, the anti-halitosis effect of a mouthwash containing 0.3% TPL oils was examined in ten periodontal patients. Volatile sulfur compounds, including hydrogen sulfide and methyl mercaptan, which are the main malodorous substances, tended to decrease after gargling with a mouthwash containing 0.3% TPL oils.The results of this study showed that essential oils indeed play a role in the treatment and prevention of oral diseases, especially periodontitis, candidiasis and halitosis, thereby suggesting the effectiveness of such essential oils.
1 0 0 0 OA ドメインシャッフリングによるフルクタナーゼキメラ酵素の作製とバイオフィルムの分解
- 著者
- 角田 衣理加
- 出版者
- 鶴見大学
- 雑誌
- 研究活動スタート支援
- 巻号頁・発行日
- 2010
デキストラナーゼとフルクタナーゼのキメラ酵素の開発は、う蝕予防に貢献できる可能性がある。S. mutans UA159株からデキストラナーゼA(dex A)遺伝子とフルクタナーゼ(fru A, fru B)遺伝子をそれぞれクローニングし、得られた酵素をデキストランおよびフルクタンと反応させ、それぞれの基質が分解したことをSomogyi-Nelson法により確認した。本研究により開発した酵素を用いて、バイオフィルムを分解できる可能性が示唆された。