著者

Mio ISHIMORI Masaki MICHISHITA Hisashi YOSHIMURA Daigo AZAKAMI Kazuhiko OCHIAI Toshiyuki ISHIWATA Kimimasa TAKAHASHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0297, (Released:2017-07-21)

被引用文献数

11

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A 7-year-old female domestic rabbit suffered from labored respiration, poor appetite, mild anemia and thrombocytopenia. Radioscopic examination revealed masses in multiple locations including the intrapleural cavity and spleen. Forty-three days after the first visit to a private veterinary clinic, the rabbit died of severe respiratory distress. Microscopically, all of the masses were composed of round to polygonal neoplastic cells with distinct cell borders that were arranged in a sheet pattern. Multinucleated giant neoplastic cells were often observed. Some neoplastic cells had phagocytozed one or more erythrocytes. Immunohistochemical staining revealed that the neoplastic cells expressed vimentin, CD204, Iba-1 and lysozyme, but not CD163. Based on the morphological and immunohistochemical findings, this case was diagnosed as disseminated histiocytic sarcoma with hemophagocytosis.

著者

Takuya Evan KISHIMOTO Shoko YASHIMA Rei NAKAHIRA Eri ONOZAWA Daigo AZAKAMI Makoto UJIKE Kazuhiko OCHIAI Toshiyuki ISHIWATA Kimimasa TAKAHASHI Masaki MICHISHITA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.7, pp.1155-1162, 2017 (Released:2017-07-07)

参考文献数

29

被引用文献数

7

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Cancer stem cells or tumor-initiating cells (TICs) are a small subpopulation of cells that have the capacity to self-renew, differentiate and initiate tumors. These cells may function in tumor initiation, aggression and recurrence. Whether spheres derived from canine rhabdomyosarcoma cells have stem cell-like properties is unclear. We induced sphere formation in the canine rhabdomyosarcoma cell lines, CMS-C and CMS-J, and characterized the spheres in vitro and in vivo. Sphere-forming cells were more resistant to vincristine, mitoxantrone and doxorubicin than adherent cells. Xenograft transplantation demonstrated that 1 × 103 sphere-forming cells derived from CMS-C were sufficient for tumor formation. The sphere assay showed that the sphere-forming cells were present in these tumors. These results suggest that the spheres derived from canine rhabdomyosarcoma cells may possess characteristics of TICs. This study provides the foundation for elucidating the contribution of TICs to rhabdomyosarcoma tumorigenesis.

著者

Shoichi WAKITANI Daigo YOKOI Yuichi HIDAKA Koichiro NISHINO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0321, (Released:2016-12-04)

被引用文献数

10

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Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study is aimed at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from -2.7 to -2.2 kb relative to the start site of Runx2 transcription in mice. In this region, the DNA methylation rate correlated negatively with Runx2 expression among mouse organs as well as among primary cultures of bone marrow from different dogs. Induction of mouse and dog mesenchymal-like cells into osteoblastic differentiation decreased the methylation rate of Runx2-DMR. Thus, in this study, we identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog.

著者

醍醐 龍馬 DAIGO Ryuma

出版者

名古屋大学博物館

雑誌

名古屋大学博物館報告 (ISSN:13468286)

巻号頁・発行日

no.28, pp.213-219, 2012

著者

Yutaka MOMOTA Junya YASUDA Mitsutaka IKEZAWA Jun SASAKI Masaaki KATAYAMA Kenji TANI Masahiro MIYABE Eri ONOZAWA Daigo AZAKAMI Katsumi ISHIOKA Toshinori SAKO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0652, (Released:2016-08-28)

被引用文献数

4

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Proliferative and necrotising otitis externa (PNOE) is a very rare disease affecting the ear canals and concave pinnae of kittens. This report describes a 5-month-old cat with PNOE. Histopathological examination confirmed the diagnosis. Treatment was initiated with local injection of methylprednisolone acetate into the lesions. The cat was subsequently treated with clobetasol propionate cream, a potent topical glucocorticoid ointment. The cat showed marked improvement. While topical treatment with tacrolimus, an immunosuppressive agent, is reported to be an effective therapy, to the best of our knowledge, this is the first report to treat PNOE with local corticosteroid therapy.

著者

Shiraishi Kouya Okada Yukinori Takahashi Atsushi Kamatani Yoichiro Momozawa Yukihide Ashikawa Kyota Kunitoh Hideo Matsumoto Shingo Takano Atsushi Shimizu Kimihiro Goto Akiteru Tsuta Koji Watanabe Shun-Ichi Ohe Yuichiro Watanabe Yukio Goto Yasushi Nokihara Hiroshi Furuta Koh Yoshida Akihiko Goto Koichi Hishida Tomoyuki Tsuboi Masahiro Tsuchihara Katsuya Miyagi Yohei Nakayama Haruhiko Yokose Tomoyuki Tanaka Kazumi Nagashima Toshiteru Ohtaki Yoichi Maeda Daichi Imai Kazuhiro Minamiya Yoshihiro Sakamoto Hiromi Saito Akira Shimada Yoko Sunami Kuniko Saito Motonobu Inazawa Johji Nakamura Yusuke Yoshida Teruhiko Yokota Jun Matsuda Fumihiko Matsuo Keitaro Daigo Yataro Kubo Michiaki Kohno Takashi

出版者

Springer Nature

雑誌

Nature communications (ISSN:20411723)

巻号頁・発行日

vol.7, 2016-08-09

被引用文献数

47

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日本人に多いEGFR変異を持つ肺腺がんの罹りやすさを決める遺伝子領域発見-免疫を司るHLA遺伝子など6遺伝子領域が関与-. 京都大学プレスリリース. 2016-08-25.

著者

南雲 大悟 ナグモ ダイゴ NAGUMO Daigo

出版者

千葉大学大学院人文社会科学研究科

雑誌

千葉大学人文社会科学研究科研究プロジェクト報告書 (ISSN:18817165)

巻号頁・発行日

vol.250, pp.58-75, 2012-02-28

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千葉大学大学院人文社会科学研究科研究プロジェクト報告書第250集「近現代東アジアにおける相互認識と表象-「脱亜」と「アジア主義」-」山田 賢編"Mutual Understanding in Modern East Asia:The Idea of 'Escape Asia' and 'Pan-Asianism'" Report on the Research Project No.250

著者

Masahiro MIYABE Azusa GIN Eri ONOZAWA Mana DAIMON Hana YAMADA Hitomi ODA Akihiro MORI Yutaka MOMOTA Daigo AZAKAMI Ichiro YAMAMOTO Mariko MOCHIZUKI Toshinori SAKO Katsutoshi TAMURA Katsumi ISHIOKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0031, (Released:2015-05-10)

被引用文献数

2 11

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G protein-coupled receptor (GPR) 120 is an unsaturated fatty acid receptor, which is associated with various physiological functions. It is reported that the genetic variant of GPR120, p.Arg270His, is detected more in obese people, and this genetic variation functionally relates to obesity in humans. Obesity is a common nutritional disorder also in dogs, but the genetic factors have not ever been identified in dogs. In this study, we investigated the molecular structure of canine GPR120 and searched for candidate genetic variants which may relate to obesity in dogs. Canine GPR120 was highly homologous to those of other species, and seven transmembrane domains and two N-glycosylation sites were conserved. GPR120 mRNA was expressed in lung, jejunum, ileum, colon, hypothalamus, hippocampus, spinal cord, bone marrow, dermis and white adipose tissues in dogs, as those in mice and humans. Genetic variants of GPR120 were explored in client-owned 141 dogs, resulting in that 5 synonymous and 4 non-synonymous variants were found. The variant c.595C>A (p.Pro199Thr) was found in 40 dogs, and the gene frequency was significantly higher in dogs with higher body condition scores, i.e. 0.320 in BCS4-5 dogs, 0.175 in BCS3 dogs and 0.000 in BCS2 dogs. We conclude that c.595C>A (p.Pro199Thr) is a candidate variant relating to obesity, which may be helpful for nutritional management of dogs.

著者

巽 久行 小林 だいご 向殿 政男 木名瀬 亮 Tasumi Hisayuki Kobayashi Daigo Mukaidono Masao Kinase Akira

出版者

神奈川工科大学

雑誌

神奈川工科大学研究報告 B 理工学編 (ISSN:09161902)

巻号頁・発行日

no.13, pp.p173-187, 1989-03

著者

Katsumi ISHIOKA Yumi SUZUKI Kana TAJIMA Sumire OHTAKI Masahiro MIYABE Mariko TAKASAKI Akihiro MORI Yutaka MOMOTA Daigo AZAKAMI Toshinori SAKO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.2, pp.173-177, 2013 (Released:2013-03-01)

参考文献数

15

被引用文献数

1 10

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Monocyte chemoattractant protein-1 (MCP-1) is a member of the C-C family chemokines, which mobilizes monocytes from bone marrow to the site of inflammation. To evaluate the clinical utility of canine MCP-1 as a blood test item, we measured serum MCP-1 concentrations in normal and ill dogs. Reference interval of canine MCP-1 was established as 115.6–176.9 pg/ml. Serum MCP-1 concentrations increased in the dogs affected with neoplastic (518.0 ± 84.8 pg/ml), inflammatory (257.0 ± 42.5 pg/ml) or other diseases (360.3 ± 45.2 pg/ml). The results showed high sensitivity of MCP-1 to detect neoplasia and inflammation. Moreover, MCP-1 increased in some cases in which C-reactive protein didn’t increase. MCP-1 might be helpful as a screening blood test marker for detection of neoplasia and inflammation in dogs.

著者

Oh Junepyo Kondo Takahiro Hatake Daigo Honma Yujiro Arakawa Keitaro Machida Takahiro Nakamura Junji

出版者

IOP Publishing

雑誌

Journal of physics. Condensed matter (ISSN:09538984)

巻号頁・発行日

vol.22, no.30, pp.304008, 2010-07-13

被引用文献数

8 6

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The effect of the local electronic modification of the graphite surface on the gas–graphite interaction has been investigated by the molecular beam scattering technique. The angular intensity distributions of He and Ar beams scattered from pristine and defect induced graphite surfaces have been measured at various surface temperatures. From the He scattering results, the cross-section for the He diffuse scattering per defect is estimated as being as much as 113 nm2. The origin of the extremely large cross-section is ascribed to the modulated electronic states of graphite around the defect based on the STM measurements, which is due to the local breaking of the π conjugated system of graphite. From the Ar scattering results, the effective mass of the graphite surface for the Ar collision has been estimated as M = 114 u, based on the analysis with the hard cube model. The new component appears in the scattering distribution of Ar for the defect induced graphite surface. The component has a larger peak position angle than that for the pristine graphite surface, indicating that the normal component of the translational energy of the Ar atom was greatly lost by the collision at the electronically modified area of graphite.

著者

吉田 英一 YOSHIDA Hidekazu 佐々木 重洋 SASAKI Shigehiro 寺田 騰 TERADA Noboru 浅沼 修一 ASANUMA Shuichi 槇原 大悟 MAKIHARA Daigo 桂田 祐介 KATSURADA Yusuke

出版者

名古屋大学博物館

雑誌

名古屋大学博物館報告 (ISSN:13468286)

巻号頁・発行日

vol.25, pp.183-200, 2009-12-25

著者

Katsumi ISHIOKA Yumi SUZUKI Kana TAJIMA Sumire OHTAKI Masahiro MIYABE Mariko TAKASAKI Akihiro MORI Yutaka MOMOTA Daigo AZAKAMI Toshinori SAKO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0169, (Released:2012-09-14)

被引用文献数

1 10

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Monocyte chemoattractant protein-1 (MCP-1) is a member of the C-C family chemokines, which mobilizes monocytes from bone marrow to the site of inflammation. To evaluate the clinical utility of canine MCP-1 as a blood test item, we measured serum MCP-1 concentrations in normal and ill dogs. Reference interval of canine MCP-1 was established as 115.6–176.9 pg/ml. Serum MCP-1 concentrations increased in the dogs affected with neoplastic (518.0 ± 84.8 pg/ml), inflammatory (257.0 ± 42.5 pg/ml) or other diseases (360.3 ± 45.2 pg/ml). The results showed high sensitivity of MCP-1 to detect neoplasia and inflammation. Moreover, MCP-1 increased in some cases in which C-reactive protein didn’t increase. MCP-1 might be helpful as a screening blood test marker for detection of neoplasia and inflammation in dogs.

著者

Ayami Ochi Takashi Takei Kayu Nakayama Chihiro Iwasaki Daigo Kamei Yuki Tsuruta Ari Shimizu Shunji Shiohira Takahito Moriyama Mitsuyo Itabashi Toshio Mochizuki Keiko Uchida Ken Tsuchiya Motoshi Hattori Kosaku Nitta

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.51, no.7, pp.759-762, 2012 (Released:2012-04-01)

参考文献数

15

被引用文献数

17 21

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We present two cases with steroid-resistant nephrotic syndrome (SRNS) and two cases with steroid-dependent nephrotic syndrome (SDNS) due to focal segmental glomerulonephritis (FSGS) who were treated with a single dose of rituximab (375 mg/m2). Although the two cases with SRNS showed no response, the two cases with SDNS achieved complete remission. The patients in whom the peripheral B-cell counts subsequently increased after the administration of rituximab demonstrated a relapse. Rituximab may be an effective treatment agent for SDNS with FSGS and the peripheral B-cell count may be a useful marker in such patients for preventing disease relapse.