著者

Yoshito Ikeda Mizuki Kawakami Yasuyuki Yamada Masayuki Munekane Kohei Sano Takahiro Mukai Taiho Kambe Nobukazu Shitan

出版者

The Pharmaceutical Society of Japan

雑誌

BPB Reports (ISSN:2434432X)

巻号頁・発行日

vol.6, no.3, pp.108-114, 2023 (Released:2023-06-14)

参考文献数

24

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Zinc is an essential nutrient with important biological functions, and its deficiency can lead to several diseases. The zinc transporter families, ZIP and ZNT, play essential roles in regulating zinc homeostasis and dynamics in the body and cells. Specifically, ZIP4 is the primary zinc transporter responsible for zinc absorption in the small intestine. Previous studies have shown that Panax ginseng (P. ginseng) extract can promote mouse Zip4 expression, and ginsenosides, including Rc and Re, enhance zinc uptake. However, the effects of other metabolites present in P. ginseng extract remain unclear. Therefore, we fractionated P. ginseng extract using chloroform, ethyl acetate, and n-butyl alcohol, and evaluated the effect of each fraction on zinc uptake using mouse Hepa and Hepa/MRE-Luc cells that stably expressed luciferase under the promoter of metal-responsive elements. Luciferase activity assays demonstrated that the chloroform (F1), ethyl acetate (F2), and n-butyl alcohol (F3) fractions increased cellular zinc uptake. In particular, F1 fraction was found to induce Zip4 mRNA and protein expressions, which significantly enhanced zinc uptake. Ginsenosides were mainly present in the F2 and F3 fractions, indicating that metabolites other than ginsenosides in the F1 fraction would enhance zinc uptake by inducing Zip4 mRNA and protein expressions. Our study offers novel insights into the molecular mechanisms underlying zinc uptake by P. ginseng.

著者

水野 基樹 川田 裕次郎 飯田 玲依 山本 真己 東 慎治 上野 朋子 山田 泰行 杉浦 幸 田中 純夫 Motoki Mizuno Yujiro Kawata Rei Iida masaki Yamamoto Shinji Higashi Tomoko Ueno Yasuyuki Yamada Miyuki Sgiura Sumio Tanaka 千葉経済大学短期大学部非常勤講師 CHIBA KEIZAI COLLEGE Part-time Lecturer

出版者

千葉経済大学短期大学部

雑誌

千葉経済大学短期大学部研究紀要 = Bulletin of Chiba Keizai College (ISSN:13498312)

巻号頁・発行日

no.4, pp.51-61,

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The psychology of motivation is a broad and loosely defined field. Furthermore, motivation is a basic psychological process. Few would deny that it is the most important focus of the micro organizational behavior. Traditionally, psychologists have not totally agreed on how to classify the various human motives, but they acknowledge that some motives are unlearned physiologically based. For that reason, it is difficult to comprehend a framework of motive concepts which can predict human behavior. Therefore, the purpose of this paper was to review the motive concept suggested by McClelland(1987), who is most closely associated with the study of achievement motive. Specifically, first we summarized up the measurement and concepts of motive, such as "measures of human motive dispositions", "achievement motive", "power needs", "affiliative motive" and "avoidance motive". In addition, we critically reviewed and discussed them in terms of the definitions of concepts. In conclusion, although we could grasp the implications of each motive concept, there were some ambiguities regarding McClelland's motive concepts. Thus, more elaborate conceptual regulation and detailed explanation of the connections to the concepts seem necessary in order to clarify the notion of motive concepts, because there are a number of motives which lie in a gray area from the viewpoint of human motivation described by McClelland.

著者

Miya Urui Yasuyuki Yamada Akira Nakagawa Fumihiko Sato Hiromichi Minami Nobukazu Shitan

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.46, no.10, pp.1494-1497, 2023-10-01 (Released:2023-10-01)

参考文献数

15

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Advancements in synthetic biology have facilitated the microbial production of valuable plant metabolites. However, constructing complete biosynthetic pathways within a single host organism remains challenging. To solve this problem, modular co-culture systems involving host organisms with partial pathways have been developed. We focused on Escherichia coli, a general host for metabolite production, and Pichia pastoris (Komagataella phaffii), a novel synthetic biology host due to its high expression of biosynthetic enzymes. Previously, we reported the co-culture of E. coli cells, which produce reticuline (an important intermediate for various alkaloids) from glycerol, with P. pastoris cells, which produce the valuable alkaloid stylopine from reticuline. However, Pichia cells inhibited E. coli growth and reticuline production. Therefore, we aimed to improve this co-culture system. We investigated the pre-culture time before co-culture to enhance E. coli growth and reticuline production. Additionally, we examined the optimal concentration of Pichia cells inoculated for co-culture and methanol addition during co-culture for the continuous expression of biosynthetic enzymes in Pichia cells. We successfully established an improved co-culture system that exhibited an 80-fold increase in productivity compared to previous methods. This enhanced system holds great potential for the rapid and large-scale production of various valuable plant metabolites.

著者

Yasuyuki YAMADA Junichi NAKATSUKA Toshio MORITA

出版者

The Japan Society of Mechanical Engineers

雑誌

Journal of Mechanical Systems for Transportation and Logistics (ISSN:18821782)

巻号頁・発行日

vol.5, no.1, pp.110-119, 2012 (Released:2012-11-07)

参考文献数

13

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Passive dynamic walking requiring little exertion is one way of realizing efficient bipedal locomotion. In this case, forces should be converted only to shape ideas for the bottom leg, which naturally alternates the vibration of the roll and yaw axes. To achieve our objectives, in this study we focus on a spinning top, and the change in the direction of movement about the rotation axis was defined as irregular motion. It was shown that this method of realizing motion axis conversion occurred during rotation. An initial angular velocity is generated on the center axis of the solid body's curved surface, and changed the motion into a backspin. First, it was confirmed that we can accurately describe a half ellipsoid as a twisted solid curved surface on its center axis. Next we changed the contact point. By adjusting the angular momentum, we also showed the method of momentum vector conversion through friction. We extracted the constraint condition, and a translational direction element of the angular momentum vector was synthesized. Finally, we showed a method for converting the irregular motion into translational motion, so that the irregular motion can realize a twist angle and an ellipsoid shape.

著者

Takeshi Ebara Ryohei Azuma Naoto Shoji Tsuyoshi Matsukawa Yasuyuki Yamada Tomohiro Akiyama Takahiro Kurihara Shota Yamada

出版者

(公社)日本産業衛生学会

雑誌

Journal of Occupational Health (ISSN:13419145)

巻号頁・発行日

pp.17-0101-OA, (Released:2017-08-24)

被引用文献数

12

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Objectives: Objective measurements using built-in smartphone sensors that can measure physical activity/inactivity in daily working life have the potential to provide a new approach to assessing workers' health effects. The aim of this study was to elucidate the characteristics and reliability of built-in step counting sensors on smartphones for development of an easy-to-use objective measurement tool that can be applied in ergonomics or epidemiological research.Methods: To evaluate the reliability of step counting sensors embedded in seven major smartphone models, the 6-minute walk test was conducted and the following analyses of sensor precision and accuracy were performed: 1) relationship between actual step count and step count detected by sensors, 2) reliability between smartphones of the same model, and 3) false detection rates when sitting during office work, while riding the subway, and driving.Results: On five of the seven models, the inter-class correlations coefficient (ICC (3,1)) showed high reliability with a range of 0.956–0.993. The other two models, however, had ranges of 0.443–0.504 and the relative error ratios of the sensor-detected step count to the actual step count were ±48.7%–49.4%. The level of agreement between the same models was ICC (3,1): 0.992–0.998. The false detection rates differed between the sitting conditions.Conclusions: These results suggest the need for appropriate regulation of step counts measured by sensors, through means such as correction or calibration with a predictive model formula, in order to obtain the highly reliable measurement results that are sought in scientific investigation.