著者

Wenchao LI Xinchao LIU Youfang GU Junlong LIU Jianxun LUO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0111, (Released:2019-05-17)

被引用文献数

27

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The cat is a reported reservoir for several zoonotic pathogens, including Cryptosporidium spp., Giardia duodenalis, and Blastocystis sp. These parasites represent a significant, but often neglected, threat to humans and animals. Furthermore, Tritrichomonas foetus has been described inhabiting the digestive tract of cats, and may be causative agents of gastrointestinal symptoms. However, scant data are available concerning the molecular epidemiology of these parasites in domestic cats in China. This study examined fecal samples from domestic cats in Eastern China to unravel the molecular epidemiology of four protozoans. Of the 346 samples examined, 47 (13.6%) were positive for the detected pathogens, including 8 (2.3%), 5 (1.4%), 2 (0.6%), and 35 (10.1%) samples positive for Cryptosporidium spp., G. duodenalis, Blastocystis sp.,and T. foetus, respectively. Co-infection with Cryptosporidium spp. and T. foetus was detected in three cats, no other mixed infections were observed. No age,sex or fecal condition predisposition was observed with any of the four pathogens. The species/ assemblages/ subtypes/ genotypes were C. felis, Assemblage A and F, ST1, and cat genotype for Cryptosporidium spp., G. duodenalis, Blastocystis sp., and T. foetus detected in this study, respectively. The presence of zoonotic species/ assemblages/ subtypes/ genotypes poses a threat to public health. These findings provide useful information for the design of prevention and control strategies to reduce the burden of protozoal infections in cats.

著者

Rina NABETA Naoki KAMBE Yuki NAKAGAWA Shiori CHIBA Hou XIANTAO Tetsuya FURUYA Miori KISHIMOTO Tsuyoshi UCHIDE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0387, (Released:2019-05-16)

被引用文献数

2

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A neutered male Golden Retriever was referred with a 2-week history of dry mouth. Multiple and bilateral enlargement of the lacrimal and salivary glands showing heterogeneous internal enhancement was identified on contrast-enhanced computed tomography (CT). Ultrasonographic examination detected multifocal hypoechoic areas within the swollen submandibular salivary glands, which were histopathologically diagnosed as lymphoplasmacytic sialoadenitis. As both imaging and histopathological findings were in accordance with those in human Sjögren’s syndrome, a provisional diagnosis of Sjögren’s-like syndrome was made. Immunosuppressive drugs promptly improved clinical signs concurrently with the abnormal sonographic findings, indicating the feasibility of ultrasonography in monitoring therapeutic outcomes. Herein, we discuss a proposed criteria set for diagnosis of Sjögren’s-like syndrome in veterinary medicine.

著者

Rommaneeya LEELA-ARPORN Hiroshi OHTA Masahiro TAMURA Noriyuki NAGATA Kazuyoshi SASAOKA Angkhana DERMLIM Khoirun NISA Keitaro MORISHITA Noboru SASAKI Kensuke NAKAMURA Satoshi TAKAGI Kenji HOSOYA Mitsuyoshi TAKIGUCHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0673, (Released:2019-04-08)

被引用文献数

2

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A definitive diagnosis of focal liver lesions (FLLs) requires invasive procedures for histopathologic examination. Thus, a simpler noninvasive diagnostic method, such as conventional ultrasonography combined with clinical data, is needed for the prediction of liver malignancy. The objective of this study was to examine the diagnostic accuracy of clinical data and ultrasonographic (US) features to differentiate benign and malignant liver lesions. Medical records and US images from dogs with FLLs that underwent abdominal US and histopathologic examinations following surgery or liver biopsy were retrospectively reviewed. Clinical data, including signalment, clinical signs and laboratory findings, and the US features of liver lesions that could act as predictive factors were assessed using univariate and multivariate analyses to evaluate the associations between predictive factors and liver malignancy. Based on the histopathologic results, 55 dogs with malignant lesions and 28 dogs with benign lesions were included in the study. The results of univariate analysis showed that several US features and platelet count were significantly associated with liver malignancy. Multivariate analysis revealed that the platelet count (thrombocytosis; odds ratio [OR]: 4.13, 95% confidence intervals [CI]: 1.81–9.41), lesion size (4.1 cm or greater; OR: 23.83, 95% CI: 3.74–151.95) and echotexture of FLLs (heterogenous; OR: 8.44; 95% CI: 1.37–51.91) were independent predictors for differentiating benign and malignant liver lesions, suggesting that a combination of clinical data and US findings of FLLs could predict liver malignancy in dogs.

著者

Takae KAWAGUCHI Rina HASHIMOTO Youko YASUKAWA Shusaku YAMADA Aritada YOSHIMURA Daiki HIRAO Takahiro OMORI Ryuji FUKUSHIMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0772, (Released:2019-04-08)

被引用文献数

3

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Maintaining a good ventricular systolic function is important in the long-term therapy of dogs with supraventricular tachyarrhythmia (SVTA). The objective of this study was to evaluate the inhibitory effect of telmisartan on myocardial injury and the resulting ventricular systolic dysfunction in a canine model of SVTA. A total of 14 dogs were randomly assigned to a Telmisartan (oral telmisartan, 1.0 mg/kg daily, n=7) or a Control (no drug administration, n=7) group; the duration of rapid atrial pacing (RAP) was 3 weeks for both groups. The cardiac troponin I (cTnI) concentration in the Control group was significantly increased after 3 weeks compared to that before RAP initiation (baseline), but no significant difference was observed in the Telmisartan group. Moreover, the cTnI concentration at 3 weeks was significantly lower in the Telmisartan group than in the Control group. The left ventricular fractional shortening was significantly decreased at 3 weeks compared to that at baseline in both groups. However, fractional shortening at 3 weeks was significantly higher in the Telmisartan group than in the Control group. The cardiac output values in the Control group were significantly decreased at 3 weeks compared with those at baseline, but no significant difference was observed in the Telmisartan group. This study demonstrates that telmisartan inhibits the reduction in ventricular systolic function and prevents myocardial injury in a canine model of SVTA. Therefore, telmisartan is suggested as a novel treatment for canine SVTA.

著者

Hidetaka NISHIDA Kohei NAKATA Sadatoshi MAEDA Hiroaki KAMISHINA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0521, (Released:2019-05-15)

被引用文献数

4

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Thoracolumbar intervertebral disk herniation (TL-IVDH) with caudal articular process anomalies has been reported in Pugs. It currently remains unclear whether congenital caudal articular process aplasia/hypoplasia predisposes to the development of TL-IVDH. However, there are difficulties in proving the causal relationship between caudal articular process anomalies and TL-IVDH. The aim of this study was to describe the prevalence of TL-IVDH at the vertebral space containing anomalous and normal caudal articular processes in Pugs. Fifty-seven pugs were eligible to be included in this study. Caudal articular process aplasia/hypoplasia affected 52/57 (91.2%) dogs. The caudal articular process anomalies were most frequently located between T10 and T13. Colocalization of caudal articular process aplasia/hypoplasia and TL-IVDH was detected in 11 dogs (19.3%). The prevalence of TL-IVDH at vertebral spaces containing abnormal caudal articular processes was 12.3%, whereas the prevalence of TL-IVDH at vertebral spaces containing normal articular processes was 2.4%. With the increase in the number of vertebrae with caudal articular process anomalies, the prevalence of TL-IVDH also increased. The results of this study suggested the prevalence of caudal articular process anomalies was high in Pugs. The caudal articular process anomalies could be associated with TL-IVDH. A large cohort is needed to prove the causal relationship between caudal articular process anomalies and TL-IVDH.

著者

Angkhana DERMLIM Tatsuyuki OSUGA Kensuke NAKAMURA Tomoya MORITA Khoirun NISA Kazuyoshi SASAOKA Rommaneeya LEELA-ARPORN Noriyuki NAGATA Masahiro TAMURA Noboru SASAKI Hiroshi OHTA Mitsuyoshi TAKIGUCHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0012, (Released:2019-05-15)

被引用文献数

7

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The purpose of this study was to evaluate the cardiac acute volume loading effect on left atrial (LA) strain and strain rate (SR) parameters derived from two-dimensional speckle tracking echocardiography (2D-STE) in healthy dogs. Six healthy beagles were anesthetized and subjected to increase cardiac preload by intravenous infusion with lactated Ringer solution at 150 ml/kg/hr for 90 min. A Swan-Ganz catheter was placed to directly measure the mean pulmonary capillary wedge pressure (PCWP). Echocardiography was performed before (baseline) and at 15, 30, 45, 60, 75, and 90 min after acute volume loading began. Apical 4-chamber images focused on the LA were digitally recorded for later strain and SR analysis via 2D-STE. Acute volume loading significantly increased from baseline during LA strain and SR as assessed by the speckle tracking–based technique during reservoir and conduit function at 15 to 90 min after volume load began, and strain indices representing booster pump function were enhanced at 45 to 90 min. In addition, acute volume loading resulted in a significantly greater PCWP after fluid infusion. On multiple regression analysis, quadratic regression analysis was a better fit for the relationship between PCWP and all LA functional indices. Our findings indicated that LA function analyzed by strain and SR was enhanced during cardiac acute volume loading in healthy dogs. The change in strain and SR during acute volume loading should be interpreted with caution during the diagnosis of heart diseases related to volume overload.

著者

Kazufumi KASUYA Nobuyuki TANAKA Fumi OSHIMA Nozomi FUJISAWA Megumi SAITO Katsunori TAGAMI Hidekazu NIWA Kazumi SASAI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0656, (Released:2019-04-24)

被引用文献数

2

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Strangles is a commonly diagnosed and important infectious disease of equids worldwide, caused by Streptococcus equi subsp. equi. We determined the SeM genotypes of S. equi isolated from imported horses at the Japanese border within the past 8 years, which allowed us to classify 12 strains isolated from these horses from each exporter into four allelic groups. These alleles were different from the alleles of past isolates found in Japan. Furthermore, four strains classified into the same allele were isolated from horses from one exporter over several years. In this study, S. equi isolates from different exporters had different SeM alleles. Attention to the hygiene status of farms will be necessary to prevent the incursion of strangles.

著者

Yasunori KOYATA Kiyoko WATANABE Toshizo TOYAMA Haruka SASAKI Nobushiro HAMADA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0067, (Released:2019-04-23)

被引用文献数

3

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Periodontal disease is a significant problem in companion animals such as dogs and cats. However, there is little information available about fimbriae association of periodontal disease in companion animals. In this study, we have purified and characterized a fimbriae from P. salivosa ATCC 49407. The molecular mass of this protein was approximately 60-kDa, as estimated by SDS-PAGE. Immunogold electron microscopy revealed that anti-60-kDa fimbrial serum bound to fimbria on the cell surface of P. salivosa ATCC 49407. However, fimbriae of P. gingivalis and P. gulae were not labeled with the same antibody. Immunoelectron-microscopic studies and immunoblot analysis revealed that antigenicity and molecular weight were distinct from previously reported Porphyromonas fimbrial proteins. The amino acid sequence of the N-terminal 15 residues of the 60-kDa fimbrillin protein revealed only 3 of 15 residues identical to other Porphyromonas species fimbrillin proteins. Thus, the N-terminal amino acid sequence of the 60-kDa fimbrillin protein of P. salivosa clearly differed from previously reported fimbrillin proteins. The level of adherence of the P. salivosa was 1.81%. It was confirmed that P. salivosa can adheres to human cells. These results suggest that the 60-kDa fimbriaeof P. salivosa ATCC 49407 is a new type of fimbria and may have an important factor in the adherence host cells. We suggest that the surface structure of P. salivosa may have a role in the colonization of this organism in periodontal pockets in companion animals.

著者

津曲 茂久 東野 利忠 高木 香 大場 茂夫 佐藤 昌介 武石 昌敬

出版者

社団法人日本獣医学会

雑誌

日本獣医学雑誌 (ISSN:09167250)

巻号頁・発行日

vol.53, no.5, pp.797-801, 1991-10-15

被引用文献数

1

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妊娠馬10頭を用いて妊娠1から11ヶ月まで, 血漿中estrogen, gestagen, cortisol (F), 13, 14-dihydro, 15-keto-PGF_2α(PGFM)および妊馬血清性性腺刺激ホルモン(PMSG)を測定した. estron (E_1)とestradiol-17β(E_2)は妊娠8ヶ月前後にピークを示し, progesterone (P)は妊娠3と11ヶ月で増加し, 17α-OH-progesterone (17α-OHP)は妊娠3ヶ月に明瞭なピークを形成した. 20α-OH-progesterone (20α-OHP)は妊娠6ヶ月より急激に上昇した. PGFMは妊娠2と11ヶ月にピークを示し, FとPMSGは妊娠2と3ヶ月にそれぞれピークを示した. 因子分析において, 妊娠月齢, E_1, E_2, 20α-OHPは妊娠進行に伴い増加する変数として第一因子に含まれた. PMSG, 17α-OHP, Pは副黄体に関係する変数として第二因子に, PGFMとFは妊娠2ヶ月における変化により, 第三因子に区分された. Pは第二因子に含まれたが, 第一と第三因子にも影響を受け, PGFMとは対立的な関係性を示した. 結論的には, 第一因子に含まれたE_1, E_2および20α-OHPは妊娠6ヶ月以降の胎盤機能の指標として有用であることが示唆された.

著者

Masaru USUI Akari KAJINO Michiha KON Akira FUKUDA Tomomi SATO Takahiro SHIRAKAWA Michiko KAWANISHI Kazuki HARADA Chie NAKAJIMA Yasuhiko SUZUKI Yutaka TAMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0144, (Released:2019-05-02)

被引用文献数

3

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The emergence and spread of aminoglycoside-resistant bacteria are a public health concern. The acquisition of the genes encoding 16S rRNA methylases, such as armA, rmtA, and rmtB, confers high-level resistance to aminoglycosides. However, the prevalence has not been well investigated in Japanese veterinary fields. To determine the prevalence of 16S rRNA methylases in animals, we detected 16S rRNA methylases genes in Gram-negative bacteria from animals. Here, we report the isolation of rmtB amd armA from two of the 446 Escherichia coli (0.5%) and one of the 103 Klebsiella spp. isolates (1.0%) from companion animals, respectively. However, none of the isolations were observed from 2445 E. coli isolates derived from livestock in Japan. The prevalence of 16S rRNA methylases in animals, especially in companion animals, should be carefully monitored in Japanese veterinary fields to avoid the spreading of the genes.

著者

Tomoyoshi KOMIYA Hiroko TORINIWA Takahiro MATSUMURA Tsutomu TAKEGAMI Tetsuo NAKAYAMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0613, (Released:2019-04-23)

被引用文献数

11

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Thirty-seven specimens of wild boar sera were collected from August 2016 to March 2018 in Ishikawa prefecture, Japan. Thirty-two specimens (86.5%) were positive for neutralizing antibodies against Japanese encephalitis virus (JEV). Eight specimens (21.6%) were positive for IgM antibodies against JEV. One sample was obtained from a wild boar captured in February during the winter season. Four other serum specimens obtained during the winter season were positive using a JEV gene-specific PCR assay. Based on IgM and PCR assays, wild boars were infected with JEV during the winter season, suggesting that the prevalence of JEV antibodies in wild boars in Ishikawa is high and JEV activity is possible during winter in this region. In addition, wild boars may play an important role in the infection cycle of JEV.

著者

Hellen Fialho HARTMANN Marília Teresa DE OLIVEIRA João Pedro Scussel FERANTI Gabriela Pesamosca CORADINI Stephanie Lanzarini ABATI Bibiana Zoppas PIEREZAN Vanessa Zanchi SARTURI Marcella Teixeira LINHARES Álvaro José Chávez SILVA Francisco Miguel Sanchez MARGALLO Anne Santos DO AMARAL Maurício Veloso BRUN

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0631, (Released:2019-04-17)

被引用文献数

4

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Mesothelioma is a very aggressive malignant tumor with low survival rates that is often diagnosed belatedly. Pericardial effusion is a common consequence in cases of mesothelioma, with pericardiocentesis and pericardiectomy indicated; therefor thoracocentesis is necessary to drain the contents no longer retained in the pericardium. The present report describes a mesothelioma – carrying dog with a history of cardiac tamponade that underwent thoracoscopic pericardiectomy and, later, thoracoscopic implantation of a fully implantable catheter to function as a thoracic drain. In the consulted literature, there is no use of a fully implantable catheter for this purpose. The authors consider that there was an improvement in the quality of life.

著者

Sooa YOON Sang-Kwon LEE Juhwan LEE Yeong-Bin BAEK Kyoung-Oh CHO Jihye CHOI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0101, (Released:2019-04-17)

被引用文献数

1

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In an 8-year-old Labrador Retriever with progressive anorexia, constipation, and depression, CT revealed intussusception of the cecum into the ascending colon and a small cecal mass showing strong enhancement on arterial phase. The ileocecocolic junction was surgically resected and histologically diagnosed as cecocolic intussusception with carcinoid tumor. The carcinoid tumor worked as a lead point of intussusception in this case. Dual phasic CT is useful to assess the presence of gastrointestinal tumors as lead points in old dogs with intussusception.

著者

Akihiro SHIBATA Rieko HARADA Masatoshi OKAMATSU Keita MATSUNO Tomoko ARITA Yasushi SUZUKI Masayuki SHIRAKURA Takato ODAGIRI Nobuhiro TAKEMAE Yuko UCHIDA Takehiko SAITO Yoshihiro SAKODA Hiroyuki OSAKA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.81, no.3, pp.444-448, 2019 (Released:2019-03-20)

参考文献数

16

被引用文献数

8

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A new reassortant H7N3 avian influenza virus (AIV) was isolated from a duck meat product that was illegally taken on board a passenger flight from China to Japan in March 2018. Sequencing analysis revealed that the H7N3 isolate, A/duck/Japan/AQ-HE30-1/2018 (Dk/HE30-1) (H7N3), was a reassortant highly pathogenic avian influenza virus (HPAIV) that contained the haemagglutinin (HA) gene of Chinese H7N9 HPAIV. Dk/HE30-1 (H7N3) possessed a novel polybasic sequence motif PEVPKRRRTAR/GLF at the HA cleavage site that has never previously been reported in H7 HPAIVs. The HA antigenicity of Dk/HE30-1 (H7N3) slightly differed from that of H7N9 HPAIVs previously reported. These findings will help further our knowledge of the circulation and genetic evolution of emerging AIVs in endemic areas.

著者

Hae Young LEE Nibedita NAHA Najeeb ULLAH Guang Zhen JIN Il Keun KONG Phil Ok KOH Hwan Hoo SEONG Myeong Ok KIM

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.70, no.8, pp.791-797, 2008 (Released:2008-09-05)

参考文献数

22

被引用文献数

7 9

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Tyrosine hydroxylase (TH) is the rate-limiting enzyme of dopamine (DA) biosynthesis, which is up-regulated by vitamin C administration. Nurr1 gene is highly expressed in brain and important for midbrain DAergic cell development and survival. But, the role of vitamin C and/or vitamin E during Nurr1 expression is yet to be known. Further, the synergistic effect of vitamins C and E on TH expression has not yet been explored clinically. Therefore, we studied the effects of single or co- administration of vitamin C (0.5 mM) and vitamin E (1 mM) for 72 hr, on both TH and Nurr1 expression in in vitro primary cultured gestational days (GD) 13.5 rat ventral mesencephalon (VM) by Western blot and immunocytochemistry. Our study revealed highest expressions of both TH and Nurr1 in the vitamin C + vitamin E treated group. TH expression was also increased in the vitamin C treated group than that of the control group, but the vitamin E treated group did not show any statistically significant effect. However, both the vitamin C and the vitamin E treated groups revealed increased expression of Nurr1 protein as compared with the control group. The present experimental data suggest that vitamin C can up-regulate the protein expression of TH, but Nurr1 level was elevated either by vitamin C or by vitamin E administration. Further, vitamin E acts synergistically with vitamin C to elevate TH and Nurr1 expression, which is the most novel finding of our study and for the first time; we reported this result, since there have been no published data on the synergistic effect of both the antioxidant vitamins on TH and Nurr1 expression in VM. As the motor function defect due to the progressive loss of DAergic neuron is the major reason of Parkinsonism, therefore, the results of our study finally suggest the effective role of vitamin C and vitamin E during early treatment of Parkinsonism.

著者

村田 浩一 柳井 徳麿 吾妻 健 宇仁 茂彦

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.65, no.8, pp.945-947, 2003-08-25

被引用文献数

1 20

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膵内分泌腺癌が原因で死亡した飼育下の成雌ユキヒョウ(Uincia uncia)の右心室内および肺動脈内にフィラリア成虫3隻の寄生を認めた.虫体の計測値は犬糸状虫(Dirofilc immitis)のものとほとんど一致しており,形態およびミトコンドリアDNA COI領域の比較においてもD.immitisと遠いが認められなかった. D.immitisはこれまでネコ科動物2属3種に感染が報告されているが,新たな宿主としてユキヒョウが加えられた.

著者

Hiro HORIKIRIZONO Kumiko ISHIGAKI Takao AMAHA Keigo IIZUKA Takahiro NAGUMO Kei TAMURA Mamiko SEKI Kazuya EDAMURA Kazushi ASANO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0575, (Released:2019-03-29)

被引用文献数

1

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This study investigated the direct effects of non-steroidal anti-inflammatory drugs (NSAIDs) and atrial natriuretic peptide (ANP) on canine-derived vascular endothelial cells (VECs). VECs were isolated and cultured from canine arteries and veins. The mRNA expressions of vascular endothelial growth factor receptor 2, cyclooxygenase-2, and natriuretic peptide receptor 1 were detected in the cultured VECs. The viability of the cultured VECs was reduced in a dose-dependent manner by meloxicam, carprofen, and robenacoxib. By contrast, dose escalations of ANP had only marginal influence on the viability of cultured VECs. NSAIDs may potentially serve as not only analgesic agents against cancerous and perioperative pain but also as adjuvant anti-angiogenic drugs in dogs with malignant tumors.

著者

Shuichiro KANAI Takuro SHIMADA Takanori NARITA Ken OKABAYASHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0049, (Released:2019-03-26)

被引用文献数

10

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Phosphofructokinase-1 (EC:2.7.1.11, PFK-1) catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using adenosine triphosphate and is a key regulatory enzyme of glycolysis. Mammalian PFK-1 isozymes are composed of three kinds of subunits (PFK-M, -L, and -P), with different properties. It has been suggested that the proportion of PFK-1 subunits in different organs is based on the organ energy metabolism. In this study, we analyzed the activity and subunit composition of canine PFK-1. We found that, in dogs, the skeletal muscle only has PFK-M, the liver mainly has PFK-L, and the brain expresses all of them. The knowledge of the composition of PFK-1 could provide useful information for determination of the differences in glycolysis in various organs of dogs.

著者

Wanchart YIPPADITR Atthaporn ROONGSITTHICHAI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0571, (Released:2019-03-26)

被引用文献数

2

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This is the first study of using sublingual mucosae to establish a new urination passage in male cats suffering from complications from prior urethrostomy. In total, five domestic male cats afflicted with various complications, such as urethra stricture, scalding dermatitis, and urinary tract infection, after urethrostomy were included in the study. Sublingual mucosa was harvested to graft with abdominal muscles and further made into a tube connecting with the remaining urethra of the bladder at the cranial end and with the prepuce at the caudal end, serving as a new passage for urination. Postoperatively, all cats were alive and well recovered with no complications both in the oral cavity and at the surgical site. Radiography with contrast medium at two weeks and six months after surgery showed no leakage, stricture, or abnormality of the new urination passage. Moreover, all cats were able to urinate from the prepuce without any difficulty or signs of discomfort.

著者

鎌田 洋一 星 英之 長木 勇人 小崎 俊司

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.64, no.9, pp.767-771, s・iv, 2002-09-25

参考文献数

22

被引用文献数

1

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ボツリヌス菌が産生するC3酵素と呼ばれるADP-リボース転移酵素は,GTP-結合タンパク質の1つであるRhoAタンパク質にADP-リボースを転移し失活させる.ヒト神経芽腫GOTO細胞をC3酵素処理するとRhoAタンパク質の修飾の後,神経突起が誘導される.私たちはC3酵素に対しモノクローナル抗体を作成し,C302と名づけ,その性状を検討した.C302はC3酵素のタンパク質分解酵素処理して生じた小さなフラグメントには反応せず,C302のエピトープは立体構造依存性であると考えられた.C302はC3酵素の生物学的活性およびADP-リボース転移酵素活性を効率良く中和した.C302のC3酵素活性抑制と生物学的活性抑制における用量依存曲線は良く類似していた.C3酵素処理によって神経突起が誘導されているGOTO細胞に対しC302を反応させたところ,GOTO細胞の形態がC3酵素処理以前に復帰した.C3酵素とC302モノクローナル抗体は,RhoAタンパク質を介する細胞現象の解析研究に役立つと考えられる.