著者

Hiroki OKANISHI Jun MATSUMOTO Sadao NOGAMI Yumiko KAGAWA Toshihiro WATARI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0518, (Released:2013-03-01)

被引用文献数

1

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An 8-year-old male Shiba dog presented with chronic vomiting and diarrhoea. Upper gastrointestinal endoscopy revealed severe enteritis and infection of the duodenal mucosa with Echinostoma hortense. We performed therapy for parasites and enteritis. The therapy was successful for deworming, and temporarily improved the symptoms, but the dog died soon thereafter. To the authors’ knowledge, this is the first case report of an ante mortem diagnosis of E. hortense infection in a dog.

著者

Takuma MIYATA Takanori KODAMA Rie HONMA Yoshinori NEZU Yasuji HARADA Takuya YOGO Yasushi HARA Masahiro TAGAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0436, (Released:2013-02-28)

被引用文献数

5 25

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To investigate influence of general anesthesia on immunological anti-tumor activity, the natural killer (NK) cytotoxic activity of peripheral lymphocytes (PBLs) was measured in 7 dogs anesthetized for 3 hr with isoflurane following propofol-induction (anesthesia group) and 6 dogs without anesthesia (control group). Blood samples were collected before (baseline) and 24, 120 and 192 hr after the anesthesia. The PBLs were isolated via centrifugation with Ficoll-Hypaque solution (density, 1.073), and adherent cells were removed. The NK cytotoxic activity of the isolated PBLs against canine thyroid cancer cells was detected by the colorimetric rose Bengal assay. Significant decrease in the NK cytotoxic activity was observed at 24 hr after the anesthesia, compared with the baseline values and the control group. The NK cytotoxic activities were recovered to the baseline values until 120 hr after the anesthesia. The general anesthesia with isoflurane following propofol-induction decreased the NK cytotoxic activities of PBLs in dogs. This finding has a clinical relevance to the risk of tumor recurrence or metastasis induced by the suppression of immunological anti-tumor activity after general anesthesia in dogs. The results further emphasized the importance of the need to evaluate immune suppression following general anesthesia in animals.

著者

森田 幸雄 丸山 総一 勝部 泰次

出版者

社団法人日本獣医学会

雑誌

日本獣医学雑誌 (ISSN:09167250)

巻号頁・発行日

vol.56, no.3, pp.475-479, 1994-06-15

被引用文献数

8

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1988年から1990年の3年間に, 群馬県内のG食肉処理場で, 処理された豚1,776,294頭中4,919頭(0.28%)に非定型抗酸菌症が発見された. 結核様結節の多くは, 下顎リンパ節(64.4%)と腸間膜リンパ節(29.0%)にみられた. 上記の食肉処理場に出荷している1,200養豚場のうち4農場に集団発生が, 870農場に散発的な発生がみられた. 1988年9月から1989年12月の間に発見された2,076頭中231頭について病変部ならびに胃, 盲腸内容からの非定型抗酸菌の分離を試みた. 結核様病変がリンパ節のみにみられたもの219頭中141頭の病変部から, 肝臓または実質臓器とリンパ節の両者にみられたもの12頭中12頭の病変からMycobacterium avium-intracellulare complex(MAIC)が分離された. また, 細菌検査を実施した231頭のうち11頭の胃内容および6頭の盲腸内容からMAICが分離された. 分離株431株のうち336株は13の血清型に分類された. MAIC血清型6型(34.6%)が最も優勢で, 次いで8型(21.8%), 4型(8.6%), 10型(6.5%)であり, MAIC血清型3型も4株検出された. 16頭の豚で複数の血清型が分離された. 群馬県ではMAICによる豚の非定型抗酸菌症が蔓延していることが示唆された.

著者

小松 武志 坪田 敏男 山本 欣郎 阿閉 泰郎 鈴木 義孝

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.59, no.7, pp.521-529, 1997-07-25

被引用文献数

15 33

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ニホンツキノワグマ(Ursus thibetanus japonicus)の精巣内でのステロイド合成酵素の局在に関する季節変化を観察することを目的に, 免疫組織化学的観察を行った. さらに, 血清中テストステロンおよびエストラジオール-17β濃度をRIA法を用いて測定し, 免疫染色の結果と比較検討した. 精細管中における精細胞の形態学的観察から, 各材料採取時期を活性期(5, 6月), 退行期(11月), 休止期(1月), 前回復期(3月)および後回復期(4月)に区分した. 血清中テストステロン濃度は精子形成活性とよく一致した季節変動を示した. Cholesterol side-chain cleavage cytochrome P450, 3β-hydroxysteroid dehydrogenase(3βHSD)および17α-hydroxylase cytochrome P450はライディヒ細胞に通年的に観察され, 3βHSD陽性細胞数のみが血清中テストステロン濃度とよく相関した変化を示した. Aromatase cytochrome P450(P450arom)は通年的にライディヒおよびセルトリ細胞で, 5月および翌年の4および6月に精子細胞で, 1および3月に筋様細胞で観察された. また, P450arom陽性ライディヒ細胞数は5月および翌年の1, 3および6月に増加したが, 血清中エストラジオール-17β濃度は特定の季節変動を示さなかった. これらの結果から, 3βHSDはライディヒ細胞におけるテストステロン産生のキー酵素であること, またライディヒおよび筋様細胞由来のエストロジェンはオートクリンもしくはパラクリン的にネガティブフィードバックによってライディヒ細胞の機能を調節していることが示唆された.

著者

Hisashi INOKUMA Shigeki MAETANI Jyunji FUJITSUKA Ai TAKANO Kozue SATO Takashi FUKUI Toshiyuki MASUZAWA Hiroki KAWABATA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0027, (Released:2013-02-22)

被引用文献数

2 8

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Two dogs that exhibited sudden astasia, anorexia and fever higher than 40°C were suspected of having Lyme disease in July 2011. Clinical symptoms gradually improved with antibiotic treatment in both cases. Polymerase chain reaction and sequence analysis revealed Borrelia garinii DNA fragments in the peripheral blood in the acute disease phase. Serological tests, including enzyme linked immunosorbent assay and Western blot analysis, showed an increased IgG antibody titer against Borrelia pathogens in one of the dogs. These findings suggested that diagnosis of the two dogs were Lyme disease related to B. garinii infection.

著者

Cheng-Shu CHUNG Naoki FUJITA Naoya KAWAHARA Sho YUI Eunryel NAM Ryohei NISHIMURA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0470, (Released:2013-02-19)

被引用文献数

12 39

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Stem cell transplantation is one of the most promising yet enigmatic treatments for spinal cord injury (SCI), a common problem in dogs. As pre-differentiated mesenchymal stem cells (MSCs) can be expanded and differentiated into neurospheres in vitro, before being transplanted back, they may prove to be more beneficial for treating SCI. Therefore, we compared the endogenous differentiation potential, including the neuronal cell differentiation, of neurospheres from canine bone marrow MSCs (cBMMSCs) with that of the adipose tissue-derived MSCs (cADMSCs). Nestin-positive neurospheres were generated from MSCs derived from the bone marrow and adipose tissue. Neuronal cells were differentiated from the neurospheres derived from both these tissues. Gene expression analysis revealed that Nestin, βIII-tubulin, NCAM, OCT4 and SOX2 were expressed in MSCs and the corresponding neurospheres. Notably, cBMMSC-derived neuronal cells expressed higher levels of βIII-tubulin. The mRNA expressions of NANOG, Nestin, OCT4 and SOX2 were upregulated in neurospheres derived from both. Immunofluorescence analysis detected the expression of neuronal markers, namely, βIII-tubulin, GFAP, S100, NF200 and MAP2, in differentiated neuron-like cells. Our findings highlight that both cBMMSCs and cADMSCs could be differentiated into neurospheres and neuron-like cells, and therefore, these cells are suitable candidates for cell transplantation. Further, cADMSCs form a more suitable cell source, as larger number of cells could be harvested from cADMSC-derived neurospheres. Future studies employing in vivo transplantation models to investigate the effectiveness of MSCs for treating SCI are warranted.

著者

Naoki MIURA Makoto FURUKAWA Yasuo MAGARI Yasuyuki MOMOI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0270, (Released:2013-02-18)

被引用文献数

1 3

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The cross-reactivity of the anti-human D-dimer antibody 1C9-6F10 to the canine D-dimer was evaluated by western blotting using purified canine fibrinogen, fibrin degradation products (XDPs) and plasma from a dog with suspected disseminated intravascular coagulation (DIC). The antibody cross-reacted with canine XDPs and plasma from the dog with suspected DIC. A latex agglutination assay using 1C9-6F10 measured the canine XDPs in dogs. In conclusion, the antibody 1C9-6F10 cross-reacted to the canine D-fragment with high specificity, but lower affinity compared with the human D-dimer. The latex agglutination assay using the 1C9-6F10 antibody was used to measure canine plasma D-dimer concentrations.

著者

Anna KOIKE Rui KANO Masahiko NAGATA Charles CHEN Cheol-Yong HWANG Atsuhiko HASEGAWA Hiroshi KAMATA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0372, (Released:2013-02-14)

被引用文献数

1 6

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Isolates of the yeast Malassezia pachydermatis obtained from skin samples of healthy dogs and of dogs with atopic dermatitis in Japan, Taiwan and Korea were molecularly characterized using intergenic pacer 1 (IGS1) region analysis. The percentage of IGS1 subtype isolates detected in healthy skin was as follows: 1A (6%), 1B (27%), 1C (11%), 2A (6%), 2B (6%), 3A (11%), 3B (6%), 3C (3%) and 3D (24%). In contrast, the most prevalent isolates detected in skin lesions of atopic dermatitis were subtype 3D in Japan and Taiwan and subtype 3C in Korea. All subtype isolates grew well on acidic medium (pH 6). However, subtype 3C and 3D isolates grew better than the other subtype isolates on medium at pH 8.

著者

Goo Moon-Jung Park Jin-Kyu Hong Il-Hwa YANG Hai-Jie YUAN Dong-Wei KI Mi-Ran HAN Jung-Youn JI Ae-Ri KIM Tae-Hwan WILLIAMS Bruce H. JEONG Kyu-Shik

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.70, no.6, pp.633-635, 2008-06-25

被引用文献数

4

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6ヶ月間に渡り胴背部における落屑とび慢性紅斑を特徴とする皮膚病を示した3歳の雌スピッツに治療を施したが,なんら改善がみられないことを理由に当症例が提出された.臨床検査においては,犬は活発で健康であった.皮膚培養では,真菌を確認できなかった.皮膚生検による組織学的検査では,基底層の水腫性変性を伴う界面皮膚炎,真皮における顕著な形質細胞の血管周囲集族と多量の形質細胞集族を伴う界面壁性毛包炎を示した.中程度のCD3陽性リンパ球が真皮表層に浸潤していた.この報告は,異型皮膚病変を伴うまれな犬種に見られた円板状紅斑性狼瘡の稀有な情報を提供するものである.

著者

Yasushi KATAOKA Chieko ITO Aya SASAKI Miki ISHII Satoko YAMASHIRO Kazuki HARADA Hiroki OCHI Takuo SAWADA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0243, (Released:2013-01-25)

被引用文献数

5 14

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The purpose of the present study was to determine the prevalence of antibiotic-resistant enterococci in dogs and cats subjected to differing antibiotic pressures, and the prevalence of vancomycin resistance genes in isolates from these animals. Enterococci were isolated from fecal samples of 65 healthy dogs and 29 healthy cats brought to animal hospitals, from rectal swabs of 73 puppies and 15 kittens from five breeders and two pet shops, and from fecal samples of 20 dogs and 9 cats that were treated with antibiotics in Nippon Veterinary and Life Science University Animal Medical Center. The rates of resistance to ampicillin among isolates from the kitten-puppy group and healthy dog-cat group were 6.8 and 4.3%, respectively. In contrast, the rates of resistance to ampicillin in enterococci from the treatment group under antibiotic pressure were 37.5%. There was a significant difference between the antibiotic-treated group and the untreated group (P<0.01). Similarly, in the treatment group, the rate of resistance to enrofloxacin was extremely high (75.0%). In comparison, in the healthy group and kitten-puppy group, the rates of resistance to enrofloxacin were 23.4 and 12.1%, respectively. Among these groups, a significant difference was also observed in the apparent resistance rates (P<0.01). Vancomycin-resistant enterococci (VRE) harboring vanA or vanB were not detected in any groups. Therefore, contamination of VRE in dogs and cats is still considered to be minimal in Japan.

著者

Maki ISHIBASHI Hideo AKIYOSHI Toshie ISERI Fumihito OHASHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0478, (Released:2013-01-25)

被引用文献数

5 9

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To verify availability of skin conductance (SC) as an indicator for the sympathetic nervous system (SNS) activity in dogs, the changes of SC and blood levels of stress-related hormones induced by drugs were compared. SC and cortisol, adrenaline and noradrenaline levels were measured in 5 dogs on 4 occasions with or without drug-induced sedation at 7 day-intervals (no treatment, intramuscular medetomidine 0.01 mg/kg, intramuscular acepromazine 0.1 mg/kg, and intravenous fentanyl 0.02 mg/kg). The fentanyl treatment produced significant higher levels of SC and plasma cortisol and adrenaline, compared with the other 3 treatments. The plasma noradrenaline level also tended to be higher following the fentanyl treatment. These results indicate that SC may reflect changes in the SNS activities in dogs.

著者

Dong-Choon AHN

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0497, (Released:2013-01-25)

被引用文献数

4 8

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To provide anatomical data on the nutrient foramen (NF), the authors observed the number, site and its index (SI), position, diameter and penetrating direction of the nutrient canal (NC) in the femur and tibia of German shepherd dogs. The femur often had more than two NF on the caudal surface with 30.0 to 86.7% of SI. The proximal NF of the femur was regarded to be the major NF, because it was present in most dogs and had the largest diameter. The tibia always had only one NF with 23.2 to 38.3% of SI on the lateral border. Their direction of the NC followed Bérard’s rule with the exception of the proximal NF of the femur. These results describe the anatomical condition of the NF and NC in the dog.

著者

Tetsuji KAWAKAMI Sanae SHIBATA Nobuo MURAYAMA Masahiko NAGATA Koji NISHIFUJI Toshiroh IWASAKI Tsuneo FUKATA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.72, no.12, pp.1615-1619, 2010 (Released:2010-12-29)

参考文献数

41

被引用文献数

21 83

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To understand species distribution, trends of antimicrobial susceptibility and prevalence of methicillin resistance in canine staphylococci in Japan, 190 coagulase-positive staphylococci (CoPS) were isolated from dogs with pyoderma in 2 Japanese veterinary referral hospitals. Using a multiplex polymerase chain reaction (M-PCR) method, two CoPS species were identified: 170 Staphylococcus pseudintermedius (89.5%) and 20 S. schleiferi subsp. coagulans isolates (10.5%). In these isolates, susceptibility to 7 antimicrobial agents was determined. Overall, the levels of susceptibility to cefalexin (CEX), amoxicillin/clavulanic acid (CVA/AMPC), minocycline (MINO), ofloxacin (OFLX), norfloxacin (NFLX), lincomycin (LCM) and clindamycin (CLDM) in S. pseudintermedius isolates were 38.2, 52.4, 34.7, 31.2, 34.1, 1.2 and 11.2%, respectively. In S. schleiferi subsp. coagulans isolates, 55% demonstrated susceptibility to CEX, 80% to CVA/AMPC, 70% to MINO, 45% to OFLX or NFLX and 30% to CLDM. None of S. schleiferi subsp. coagulans isolates was susceptible to LCM. To determine the prevalence of methicillin-resistant strains, we used a PCR method, which enabled detection of the fragment of mecA gene in 66.5% (113 of 170) in S. pseudintermedius and 30.0% (6 of 20) in S. schleiferi subsp. coagulans isolates. The frequencies of susceptibility to CEX, CVA/AMPC, OFLX, NFLX and CLDM were significantly lower in methicillin-resistant CoPS than in methicillin-susceptible CoPS isolates. These data suggest a high level of methicillin resistance in staphylococci isolated from dogs with pyoderma in Japan.

著者

Sungjin CHOI Jongil LEE Kazuyo IGAWA I-li LIU Muneki HONNAMI Shigeki SUZUKI Ryohei NISHIMURA Ung-il CHUNG Nobuo SASAKI Manabu MOCHIZUKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0244, (Released:2013-01-21)

被引用文献数

1 2

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In this study, we aimed to determine the effect of trehalose coating and the optimal dose of basic fibroblast growth factor (bFGF), an osteoinductive protein, loaded onto tailor-made bone implants for implant-induced bone formation in vivo. We fabricated tailor-made α-tricalcium phosphate bone implants (11 mm diameter with 2 parallel cylindrical holes). bFGF 0, 1, 10, 100 or 200 μg/implant was incorporated into implants with and without a trehalose coating, and these were subsequently implanted into dogs to correct temporal bone defects of the same size and shape. Four weeks after implantation, we analyzed the bone implants and surrounding tissues by using micro-computed tomography imaging and histological analyses, as well as gross evaluation. No significant difference in new bone formation was observed between implants with and without trehalose coating at any of the bFGF doses. Bone implants with 100 and 200 μg bFGF showed significantly more new bone formation at the implant site and within the cylindrical holes of the implants than those without bFGF (P<0.05). However, heterotopic bone formation on the skull near the implant was observed in the group that received 200 μg bFGF. These results suggest that 100 μg bFGF is the optimal dose for this implant in dogs, and that the trehalose coating may not be necessary in vivo, probably due to the presence of blood proteins and electrolytes at the implant site.

著者

Katsuhito SONE Hideo AKIYOSHI Junichiro SHIMIZU Zheng CAO Yijyun LI Toshiyuki TANAKA Akiyoshi HAYASHI Shunji SUGII Fumihito OHASHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0255, (Released:2013-01-17)

被引用文献数

1 6

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Pulmonary surfactant protein A (SP-A) is used as a biomarker to understand the clinical features of pulmonary diseases and associated prognostic indices in human medicine. This study was conducted to investigate whether or not serum SP-A concentration can be used as a biomarker for identifying pulmonary parenchymal diseases in dogs. Thirty-two dogs with pulmonary parenchymal diseases, 34 with nonrespiratory diseases and 57 healthy dogs were included. Serum SP-A concentration was measured in all dogs using sandwich enzyme linked immunosorbent assay with an anti-dog SP-A polyclonal antibody. Median serum SP-A concentration in healthy dogs was <2.0 ng/ml, whereas that in dogs with aspiration pneumonia (n=11), primary lung tumors (n=9) and blunt traumatic lung injury (BTLI; n=12) was 3.1, 7.2 and 2.6 ng/ml, respectively; these values were significantly higher than those in healthy dogs. The serum SP-A concentration in dogs with nonrespiratory diseases was comparable with that in healthy dogs. No correlation was observed between the serum SP-A and plasma C-reactive protein concentrations in dogs with aspiration pneumonia and BTLI. There was a significant correlation between the serum SP-A concentration and thoracic radiographic changes in dogs with BTLI. These findings suggest that the serum SP-A concentration may be a useful clinical biomarker of alveolar damage that can be used for differential diagnosis of pulmonary parenchymal diseases and nonrespiratory diseases in dogs.

著者

松澤 利明 野村 護 海野 隆

出版者

社団法人日本獣医学会

雑誌

日本獣医学雑誌 (ISSN:09167250)

巻号頁・発行日

vol.55, no.3, pp.351-362, 1993-06-15

被引用文献数

8

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日本製薬工業協会加盟の67社より提供されたラット約7,000匹/性, イヌ約5,000匹/性, サル約700匹/性の臨床病理検査の背景データを解析し, 最も普及している測定方法で得られたデータの加重平均と標準偏差を求め, 正常値を変動させる要因と採材条件あるいは測定方法等との係わりを考察した. 赤血球数(RBC), ヘマトクリット(Ht), ヘモグロビン(Hb), 網状赤血球数(Rt), 血小板数, 白血球数(WBC), 白血球百分率(%WBC), 凝固時間(APTT, PT), GOT, GPT, ALP, LDH, グルコース, コレステロール, トリグリセライド(TG), 総蛋白質, アルブミン, 尿素窒素(UN), クレアチニン, ナトリウム(Na), カリウム(K), カルシウム(Ca), クロライド(Cl), 無機リン(Ip), CPKについて検討した. 種差はRBC, Ht, Rt, 血小板数, WBC, %WBC, ALP, LDH, グルコース, コレステロール, TG, 総蛋白質, UN, クレアチニン, Ca, Ip, CPKでみられた. ラットの系統差は血小板数, WBC, GOT, ALP, UN,クレアチニン, CPKでみられた. 性差はHb, Ht, WBC, ALP,グルコース, コレステロール, TG, 総蛋白質, A/G比, UN, Ipでみられた. 年齢差はRBC, Hb, Ht, Rt, %WBC, GOT, GPT, ALP, LDH, コレステロール, TG, 総蛋白質, Ip, TG, Ip, CPKでみられた. 絶食・給餌の影響はAPTT, PT, ALP, グルコース, TG, UNでみられた. ラットの採血部位ではHt, WBC, CPK, Kの値に差がみられた. 血漿と血清試料の差はLDH, CPKでみられ, 血清の測定値は血漿値に比べばらつきが大きかった.

著者

Takaharu ITAMI Kodai KAWASE Naomichi TAMARU Tomohito ISHIZUKA Jun TAMURA Kenjirou MIYOSHI Mohammed A. UMAR Hiroki INOUE Kazuto YAMASHITA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0208, (Released:2013-01-07)

被引用文献数

5 14

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Tramadol is an atypical opioid analgesic widely used in small animal practice. This study was designed to determine the effect of a single intravenous (IV) dose of tramadol on the minimum alveolar concentration (MAC) of sevoflurane in dogs. Six beagle dogs were anesthetized twice to determine the sevoflurane MAC with or without an administration of tramadol (4 mg/kg, IV) at 7 days interval. The sevoflurane MAC was determined using a tail clamp method in each dog ventilated with positive pressure ventilation. The tramadol administration produced a significant reduction in the sevoflurane MAC by 22.3 ± 12.2% (1.44 ± 0.28% with tramadol versus 1.86 ± 0.30% without tramadol, P=0.010). This MAC reduction had been determined from 122 ± 19 to 180 ± 41 min following the tramadol administration. During this period, the plasma concentrations of tramadol and its metabolite, O-desmethyltramadol (M1), decreased from 429 ± 64 to 332 ± 55 ng/ml and from 136 ± 24 to 114 ± 68 ng/ml, respectively, but these changes were not statistically significant. There was no significant difference in heart rate, mean arterial blood pressure and SpO2 between the control and tramadol treatment. The dogs that received tramadol treatment sometimes breathed spontaneously. Therefore, their respiratory rates significantly increased, and PETCO2 decreased during the MAC determination. In conclusion, the single IV dose of tramadol produced a significant reduction in the sevoflurane MAC in dogs.

著者

Hiroko MIZOOKU Yumiko KAGAWA Kazuya MATSUDA Minoru OKAMOTO Hiroyuki TANIYAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0447, (Released:2012-12-28)

被引用文献数

6

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Histological and immunohistochemical evaluation of lobular dissecting hepatitis (LDH) were performed in nine American cocker spaniel dogs. Histological examination showed diffuse fibrosis with weak inflammatory reaction of extensive neutrophils, macrophages, lymphocytes and plasma cells. Immunohistochemical examination revealed the myofibroblastic cells positive to anti-α-smooth muscle actin and anti-α-vimentin antibodies produced reticular and collagen fibers and brought about the dissection of hepatic cords and diffuse disappearance of hepatocytes. Reticular fibers invading between hepatocytes and the surrounding small group of hepatocytes were strongly positive for anti-collagen type Ⅲ and anti-collagen type Ⅳ antibodies. The positivity to anti-fibronectin and anti-laminin antibodies was frequently continuous on the basement membrane of the sinusoids of the remaining hepatic cords and between the hepatocytes. Positive findings for anti-E-cadherin antibody were not observed between the hepatocytes showing the positive findings to anti-collagen type Ⅲ and anti-collagen type Ⅳ antibodies. These results may explain the expression of fibronectin and laminin that occurs prior to the invasion of reticular fibers between the hepatocytes. The present study further suggests that expression of an extracellular matrix mainly containing of fibronectin and laminin between the hepatocytes and proliferation of collagen fibers and reticular fibers has a major role in the rupture of the hepatic cords and disappearance of hepatocytes.

著者

Kanchana POONSUK Rungtip CHUANCHUEN

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.12, pp.1575-1582, 2012 (Released:2012-12-28)

参考文献数

34

被引用文献数

6 19

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As study of multidrug efflux pumps is a crucial step for development of efflux pump inhibitors for treatment of Pseudomonas aeruginosa infection, the objective of this study was to examine the contribution of the MexXY multidrug efflux systems and other chromosomal mechanisms in aminoglycoside (AMG) resistance in P. aeruginosa isolated from dogs and cats. Thirteen Pseudomonas aeruginosa isolates from canine and feline infections were examined for contribution of the MexXY multidrug efflux pump and four other chromosomally-encoded genes including PA5471, galU, nuoG and rplY to AMG resistance. All the isolates were resistant to multiple AMGs and expressed mexXY. Deletion of mexXY caused 2- to 16-fold reduction in AMG MICs. Overproduction of MexXY did not fully account for the observed AMG resistance. No good correlations were detected between MexXY transcription level and AMG MICs. While no mutations were found in mexZ, PA5471 expression varied and its impact on MexXY expression and AMG resistance is diverse. No mutations were found in galU. Only two isolates carried a single base change G-367-T in rplY. Complete transcription of nuoG was detected in all the isolates. In conclusion, the MexXY multidrug efflux pump plays a role in AMG resistance in the dog and cat P. aeruginosa isolates, while disruption of nuoG, rplY and galU did not have a significant impact. These results indicate the existence of uncharacterized AMG-resistance mechanisms.

著者

Mari VAINIONPÄÄ Marja RAEKALLIO Elina TUHKALAINEN Hannele HÄNNINEN Noora ALHOPURO Maija SAVOLAINEN Jouni JUNNILA Anna HIELM-BJÖRKMAN Marjatta SNELLMAN Outi VAINIO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.12, pp.1539-1544, 2012 (Released:2012-12-28)

参考文献数

30

被引用文献数

1 15

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The objective of this study was to compare the method of thermography by using three different resolution thermal cameras and basic software for thermographic images, separating the two persons taking the thermographic images (thermographers) from the three persons interpreting the thermographic images (interpreters). This was accomplished by studying the repeatability between thermographers and interpreters. Forty-nine client-owned dogs of 26 breeds were enrolled in the study. The thermal cameras used were of different resolutions-80 × 80, 180 × 180 and 320 × 240 pixels. Two trained thermographers took thermographic images of the hip area in all dogs using all three cameras. A total of six thermographic images per dog were taken. The thermographic images were analyzed using appropriate computer software, FLIR QuickReport 2.1. Three trained interpreters independently evaluated the mean temperatures of hip joint areas of the six thermographic images for each dog. The repeatability between thermographers was >0.975 with the two higher-resolution cameras and 0.927 with the lowest resolution camera. The repeatability between interpreters was >0.97 with each camera. Thus, the between-interpreter variation was small. The repeatability between thermographers and interpreters was considered high enough to encourage further studies with thermographic imaging in dogs.