著者
小暮 正久
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.19, no.4-6, pp.161-166, 1973 (Released:2010-03-12)
参考文献数
26
被引用文献数
1 1

When group O red cells were incubated with UDP-Galactose and serum from group B or AB subjects, they became strongly agglutinable by human anti-B serum. Agglutination titers of these converted red cells against human anti-B serum (1:512) were 1:128.Group O red cells incubated with UDP-Galactose and serum from group Bm or A1Bm subjects were converted to B active cells. Agglutination titers of these converted red cells against human anti-B serum (1:512) were 1:8 or 1:16.Comparison of the agglutination titers of the converted cells against human anti-B serum shows that the activity of serum α-galactosyltransferase from group Bm subjects is approximately one-eighth or one-sixteenth of that from group B subjects.When group O red cells were incubated with UDP-Galactose and serum from group Cis AB subjects, they could not be agglutinated by human anti-B serum. The activity of serum α-galactosyltransferase from group Cis AB subjects could not be demonstrable by agglutination test. This is considered to be associated with the regularly present cold anti-B agglutinins in the sera of group Cis AB subjects.
著者
北濱 睦夫 山口 英夫 硲 文雄 大久保 康人
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.13, no.3, pp.81-91, 1966 (Released:2010-03-12)
参考文献数
37

A 38-year-old healthy Japanese woman Mrs. N. has as much B and H substances in her saliva as have normal B individuals, but the B antigen in her red cells is very weak and H is undetectable.Her serum contains weak anti-H. It was considered that Mrs. N. 's blood might be called Bh at the phenotypical level; the suffix “h” is intended to indicate a phenotype lacking the H substance.
著者
三谷 孝子 橋本 浩司 千葉 眞彰 池淵 研二 関口 定美
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.42, no.6, pp.294-298, 1996-12-01 (Released:2010-03-12)
参考文献数
17
被引用文献数
2 1

Bacterial contamination was observed in a platelet concentrate obtained by apheresis using COBE SPECTRA and stored for 45 hours with agitation at 20 to 24°C. The platelet product was contaminated with 107 CFU/ml of Bacillus cereus. The contamination was likely attributable to bacteria on the skin at the phlebotomy site, because 1) apheresis was performed in a closed system and 2) Bacillus cereus are known to exist ubiquitously including the skin surface, and to be resistant to the chlorohexidine-ethanol that is routinely used for disinfection of phlebotomy site in our blood center. In a spike experiment with apheresis platelets, the growth of Bacillus cereus was vey rapid, with a doubling time of 6 hours to reach 107 CFU/ml in 2 days.Leukocyte depletion resulted in reduced phagocytic activity and allowed rapid growth of the contaminating bacteria in platelet products. There findings enpharize the importance of preventing as far as possible. In addition, platelet recipients, largely comprising leukemia patients, are vulnerable to infection.
著者
森 和久
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.8, no.3-4, pp.310-326, 1962 (Released:2010-03-12)
参考文献数
74

This experiment was attempted to bring to light the production mechanism of auto-antibody, homologous and heterologous antibodies to red cells, as well as the destruction mechanism in vivo of red cells caused by the presence of these antibodies.Rabbits were used for the experiment.Consideration was advanced principally concerning the determination of the activity value of Cr51 labelled on red cells, availing of the specific and convenient nature of isotope Cr51 and red cells in the ability of association and dissociation with each other, and at the same time a serological experiment on the serum was jointly performed for the consideration.The survival time in vivo of the same typed as well as different typed red cells was measured after the rabbit's blood was typed with antisera, with the result that the loss curve of both the same type and the different type cells practically coincided with that of the auto-cells.The repeated infusion of enzyme (trypsin) treated auto-cells into normal and splenectomized rabbits reveals an increased rapidity in the loss of the trypsin treated auto-cells with added frequency of the repetition. On further examining the serum of this particular rabbit, the presence of an antibody to auto-cells was proved.The experiment made on a splenectomized rabbit by infusion homologous red cells of the same as well as different types, heterologous cells and trypsin treated auto-cells, made it clear that there was no significant difference in the loss curve of homologous cells, but that the losing speed of heterologous cells from the blood flow was extra rapid during the first 30 minutes of its infusion, and also that thereafter the loss curve was obtained almost in parallel with that of a normal rabbit.With regard to trypsin treated cells it was confirmed that its repeated infusion caused reduction of the loss curve and production of an auto-antibody, regardless of the presence or non-presence of spleen.The distribution of the infused normal auto-cells, trypsin treated auto-cells and heterologous cells was examined in heart blood, liver, spleen and kidney to find that the distribution of heterologous cells in the sysem was considerably different from that of homologous cells.Of the blood cells denatured by trypsin treatment, the homologous cells and the heterologous cells differ remarkably in the way they are destroyed and disposed, but the homologous cells which have been subjected to a powerful irreversible change are disposed in the liver in compensation of the spleen, same as heterolgoous cells.
著者
土谷 太郎 蔵田 元二 横山 三男 高橋 進
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.9, no.1, pp.18-24, 1962 (Released:2010-03-12)
参考文献数
23
被引用文献数
2

Various examples of the R0R0 (-D-/-D-) genotype have been found in England, U. S. A., Japan and Puerto Rico.The present paper reports studies of an additional family.Characteristics of these bloods as established by previous studies were also seen in our cases, and it seems to reveal a complex array of Rh antibodies.
著者
村井 弘之
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.47, no.3, pp.363-368, 2001-07-01 (Released:2010-03-12)
参考文献数
19
被引用文献数
1
著者
斎藤 健一 安武 幹智 芹澤 領 丸山 みどり 竹内 久彌 小尾 俊一 小畑 清一郎 浅野 茂隆 高橋 恒夫
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.44, no.1, pp.12-19, 1998-02-01 (Released:2010-03-12)
参考文献数
16
被引用文献数
1

Banking of human placental/umbilical cord blood (PCB) requires volume reduction for prevention of hemolysis-related side effects and cost-effective storage. We collected, separated, and cryopreserved PCB following the New York Blood Center's protocol, and obtained PCB with an mean volume of 70.0±28.1ml (n=100). Red cells were sedimented by addition of 1% hydroxyethyl starch with gentle centrifugation. Recovery of nucleated cells (NC) and colonyforming units (CFU) was 82.9±13.7% and 90.4±18.9%, respectively (n=25). Volume of the NC suspension was reduced to 20ml by centrifugation and 5ml of 50% dimethylsulfoxide and 5% Dextran 40 solution were added slowly. The frozen cells were washed with a solution containing 10% Dextran 40 and 5% human serum albumin. NC and CFU recovery was 88.8±7.13% and 81.2±20.3%, respectively (n=18). No aggregation of cells occurred after the washing procedure.We studied the effect of storage after collection, cell separation, and cryopreservation under various conditions. Results suggested that collected PCB should be prestored at 25°C for less than 24 hours, and that HES of moleculer weight 400, 000 should be used at the concentration of 1-4% for efficient cell separation. Further, the cryoprotective solution could maintain the number of NCs, CFUs, and CD34+ cells under slow cooling.