著者

阿久津 智子 松村 一利 田中 和佳 渡邊 賢 櫻田 宏一

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.19, no.2, pp.103-110, 2014 (Released:2014-07-30)

参考文献数

9

被引用文献数

3 6

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OC-Hemocatch (HC), an immunochromatographic test device for fecal occult blood, has been used for the forensic identification of human blood. However, an improved kit, OC-Hemocatch S (HC-S), is currently available and HC was discontinued. Therefore, the applicability of HC-S for the forensic identification of human blood was evaluated. In addition, false-positive reactions with disinfectants and detergents, which were observed when using HC, were tested in HC-S. Similar to HC, HC-S was highly sensitive and specific for the detection of human blood. False-negative results due to high concentrations of human hemoglobin were not observed in 100-fold diluted blood. However, distilled water was not suitable as a diluent. The effects of heating treatment of blood and bloodstains and long-term storage of bloodstains on HC-S were similar with HC. However, no false-positive results were observed when disinfectants and detergents were used for HC-S. In conclusion, HC-S is a suitable and highly efficient test kit for the forensic identification of human blood.

著者

伊澤 光 古川 明 丸山 澄 堤 博文 小室 歳信

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.24, no.1, pp.89-97, 2019 (Released:2019-01-31)

参考文献数

12

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We experienced a case of successful identification of an unknown body found at breakwater based on root canal treatment. After matching the dental findings of the body to the treatment history of individual's dental records, 23 teeth showed agreement in findings. Although 8 teeth did not agree in findings, they were consistent in terms of dental treatment history. There was inconsistency in the remaining tooth. This tooth was determined as intact, but the dental records indicated the existence of a resin composite restoration on that tooth. However, that inconsistency never became a critical determinant factor. Comparison of periapical radiographs of the body with the dental records revealed that the right mandibular first premolar teeth showed considerable similarity to the images of a broken endodontic instrument and a alveolar bone resorption caused by the leakage of root canal sealer at the middle of the root. Given the above information, we concluded that the identification as the same individual is reasonable. It was thought that a case where the findings of a dental medical accident helped to confirm the identity was unusual.

著者

木下 勝博

出版者

日本法科学技術学会

雑誌

日本鑑識科学技術学会誌 (ISSN:13428713)

巻号頁・発行日

vol.6, no.2, pp.65-83, 2002

被引用文献数

1

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&nbsp;&nbsp;Tracking phenomenon, to put it plainly, is the phenomenon to form the carbonized electrical track and lose insulation in the part with potential difference on the surface of insulating materials. Tracking resistance means the difficulty occuring the tracking phenomenon of the organic insulation materials. Studies on the tracking phenomenon and the method of evaluating tracking resistance has been often reported on so far. But, there are only a few comprehensive researches and commentaries which include tracking phenomenon and the fire which occurs by the tracking phenomenon. Hence, I decided to explain tracking phenomenon of the organic insulating materials and the phenomenon that developed concerning the fire which develops and is called, tracking fire. Tracking phenomenon is discussed first. Next, cases of tracking fire in electric wiring and the wiring utensil and the studies on the cause of tracking fire are explained. Furthermore, prevention of tracking fire and future problems are also discussed.<br>

著者

財津 亘 金 明哲

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

pp.715, (Released:2017-01-10)

参考文献数

39

被引用文献数

2 1

---

Author identification through text-mining aims to judge whether an author suspected of writing a certain text is same as that of control texts. This study examined the validity of scoring for author identification. In one unit of analysis, we conducted 18 analyses (six writing styles×three multivariate analyses) across one suspected text of a blogger, one control text of a blogger, and irrelevant texts of four bloggers. The writing style factors were (1) rate of usage of non-independent words, (2) bigram of parts-of-speech, (3) bigram of postpositional particles, (4) positioning of commas, (5) rate of usage of Kanji, Hiragana et al., and (6) sentence length. We completed (1) principal components analysis, (2) corresponding analysis, and (3) multi-dimensional scaling. We obtained scores from arrangements of texts on two dimensions, convex hull polygon (CHP) consisting of control texts was overlapped with that of irrelevant texts (a score of 0). Besides not overlapping each CHP of control and irrelevant texts, (a score of +2) a suspected text arranged into CHP of control texts, (a score of +1) one not arranged into CHP of control texts but near a control text, and (a score of −1) one near an irrelevant text. We totaled the scores in one unit of analysis (18 results) and analyzed the total scores of the 240 units of analysis for 10 bloggers under the following design: 2 (author combination of suspected and control texts: same, different)×4 (number of characters: 250, 500, 1000, 1500)×3 (number of control and irrelevant texts: 3, 6, 9). The results indicated the scoring method was able to identify the authors. AUCs of number of characters were statistically significant, but the number of texts was not significant. Furthermore, rate of usage of non-independent words and parts-of-speech were quite useful to identify authors.

著者

安達 美和 高橋 知行

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.12, no.1, pp.45-51, 2007 (Released:2007-06-27)

参考文献数

7

被引用文献数

2 1

---

In the case of food adulteration or suicidal swallowing using dishwashing detergents, several extraction methods were evaluated to extract anionic, nonionic and amphoteric surfactants. Six extraction procedures: diatomaceous earth (ChemElut/chloroform or ethyl acetate), ion-pair liquid-liquid extraction (with methylene blue/chloroform), mixed-mode solid-phase extraction (SPEC PLUS DAU, C18 plus cation exchanger), C18-based solid-phase extraction (Sep-Pak Plus C18) and polymer-based reverse phase extraction (Oasis HLB) were tested. Eleven surfactants: fatty acid alkanolamide (AA), alkyl ether sulfate (AES), alkylglycoside (AG), alkylamine oxide (AO), alpha-olefin sulfonate (AOS-C=C), hydroxy-alkane sulfonate (AOS-OH), alkylamide propylbetaine (APB), fatty acid (FAT), alkylhydroxysulfobetaine (HSB), alkylbenzenesulofonate (LAS), and polyoxyethylene alkyl ether (POE), were extracted from spiked water and highest recoveries (91% for AES to 101% for AO) were obtained with Oasis HLB. On the assumption of food adulteration or stomach contents analysis, soy bean paste “miso” soup, yogurt and curry were spiked with dishwashing detergents and extracted using Oasis HLB. Extraction recoveries from these spiked foods were decreased (1% for LAS and FAT from yogurt to 78% for AO from curry) compared to extraction from spiked water. Except extraction of FAT from miso soup, protein precipitation by ethanol prior to Oasis HLB extraction was effective to improve extraction recoveries (67% for AA from yogurt to 101% for AA from miso soup).

著者

安達 美和 高橋 知行

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.10, no.2, pp.99-109, 2005 (Released:2007-07-03)

参考文献数

10

被引用文献数

4 3

---

High-performance liquid chromatography/mass spectrometry (LC/MS) was applied to the forensic discrimination of dishwashing detergents. Each dishwashing detergent, commercialy available in Japan, consists of anionic, nonionic, amphoteric surfactants or a combination of these surfactants. These surfactants were separated on a reversed-phase C18 column with methanol/10 mM ammonium acetate gradient mobile phase and were identified according to their molecular-related ions and retention times. Sixty-three commercial products were analyzed and 1,928 of all possible 1,953 pairs (ca. 99%) were distinguished without using other analytical methods. All of the indistinguishable products (25 pairs) were variation of products from a same manufacture with different fragrance, and/or concentration, and a product of original equipment manufacturer (OEM). These results indicate that LC/MS is a reliable tool for the forensic discrimination of surfactants in dishwashing detergents.

著者

瀬戸 康雄 井浦 一光 金森 美江子

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.10, no.1, pp.49-61, 2005 (Released:2007-12-10)

参考文献数

20

被引用文献数

5 5

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Gas chromatographic mass-spectrometric analysis was performed for chemical warfare agents and related compounds including sarin, soman, tabun, VX, mustard gas, lewisite 1, 2-chloroacetophenone, o-chlorobenzilidenemalononitrile and capsaicin, under electron ionization and methane chemical ionization conditions using apolar and polar capillary columns. It was possible to identify the tested compounds with respects to their retention indices and mass spectra. Under the analytical conditions of split ratio (50:1), electron ionization and scan mode data aquisition, the limit of detection ranged from 0.06 to 7 μg/ml, except for the low detection sensitivity of lewisite 1.

著者

高津 正久 福島 正雄 Ulrich Gerber

出版者

日本法科学技術学会

雑誌

日本鑑識科学技術学会誌 (ISSN:13428713)

巻号頁・発行日

vol.6, no.1, pp.57-63, 2001 (Released:2009-11-27)

参考文献数

7

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Numerous kinds of fingerprint powder such as aluminum flake have been successfully used for latent fingerprint detection in crime scene investigation. These fingerprint powders are hazardous to human health, especially for crime scene investigators, and sometimes seriously damage valuable furniture, electronic devices and precision instruments.   In this paper, dogtooth violet starch, wheat flour, black tea, green tea, green laver were ground into fine particles less than 80 micrometers using a ball mill grinder. The shape of these fine powders were observed by scanning electron microscope (SEM), and their adherence to the skin ridge deposits was evaluated. Each powder detected a latent fingerprint 4 hours after imprinting on a glass plate, but they did not give good results for older or weaker fingerprints.   Green laver, which is a marine plant of the green algae family, has a single cell layer structure. An improved green laver powder, produced by freeze-drying followed by grinding, contains a remarkable amount of thin plate particles. This characteristic feature of the improved green laver powder made it more adhesive, revealing even weak latent fingerprints 3 days after imprinting on a glass plate. The quality of lifted fingerprints was good enough to be used for fingerprint identification.

著者

久土 智也 木口 昭夫 藤井 広志

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

pp.758, (Released:2019-06-14)

参考文献数

14

---

Illegal distribution of cannabis products, such as cannabis-infused foods and e-liquids, are increasing in Japan. The main component of cannabis is tetrahydrocannabinol (THC). THC extracted from cannabis is regulated by the Cannabis Control Act, and chemically synthesized THC is regulated by the Narcotics and Psychotropics Control Act; therefore, it is important to identify the source of THC for judicial appraisal. Recently, the crime of cultivating cannabis has occurred frequently in Japan. Illegal cultivation is difficult to discover because there are cases in which cannabis is cultivated secretly in a room in apartments, etc. Cannabis is a plant that emits unique odors. Detection of the odor constituents can provide a breakthrough for the criminal investigation of cannabis cultivation. The purpose of this study was to identify cannabis products and utilize them for the criminal investigation of cannabis cultivation. Cannabis plants were cultivated from seeds and analyzed using both solid-phase microextraction (SPME) and solvent extraction. Fifty kinds of volatile components were detected by SPME and 21 kinds of refractory components were detected by solvent extraction in fresh cannabis plants. We found that terpenes were detected more strongly in cannabis buds than in cannabis leaves. Furthermore, sesquiterpenes were strongly detected from cannabis leaves and monoterpenes were strongly detected from cannabis buds. The most abundant components were β-myrcene, cis-β-ocimene, terpinolene, β-caryophyllene, trans-α-bergamotene, humulene and β-farnesene. Additionally, THC, cannabichromene, cannabigerol and tetrahydrocannabivarin known as cannabinoids, were detected in all the samples. For the identification of cannabis products, 7 kinds of terpenes and 4 kinds of cannabinoids are promising combinations. Additionally, these terpenes may be useful in the criminal investigation of cannabis cultivation.

著者

金森 達之 岩田 祐子 瀬川 尋貴 山室 匡史 桑山 健次 辻川 健治 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.24, no.2, pp.123-133, 2019 (Released:2019-07-31)

参考文献数

13

---

The isomers of fluoro-butyrylfentanyl, fluoro-isobutyrylfentanyl, and fluoro-methoxyacetylfentanyl, in which the position of fluorine on the N-phenyl ring varies, were synthesized, characterized, and differentiated by infrared (IR) spectroscopy, liquid chromatography/mass spectrometry (LC/MS), and gas chromatography/mass spectrometry (GC/MS). The isomers could be clearly differentiated by their IR spectra. In the LC/MS chromatograms, the separation of the fluoro-butyrylfentanyl and fluoro-isobutyrylfentanyl isomers was insufficient. However, in the GC/MS extracted ion chromatograms, all compounds were completely separated. The LC/MS and GC/MS mass spectra of the isomers were similar, demonstrating that it is difficult to distinguish the positional isomers of fluorinated fentanyl analogs by their mass spectra.

著者

Takaya Murakami Yoshiaki Iwamuro Reiko Ishimaru Satoshi Chinaka Ippei Noda Shuhei Higashibayashi Nariaki Takayama

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.22, no.2, pp.133-143, 2017 (Released:2017-07-27)

参考文献数

22

被引用文献数

5

---

We present herein a practical methodology for elucidating the o-, m-, or p-fluorine substitution pattern of indazole-type synthetic cannabinoids containing a fluorobenzyl group at the N-1 position and a carbonyl group at the C-3 position via electron ionization-triple quadrupole mass spectrometry. We synthesized, as model compounds of the synthetic cannabinoids, the o-, m-, and p-fluorine positional isomers: 1-[1-(2-, 3-, and 4-fluorobenzyl)-1H-indazol-3-yl]ethanone (o-, m-, and p-FUBINAE). Mass spectral analyses showed that the three isomers differed significantly in the logarithmic values of the abundance ratios of the product ion at m/z 109 to the precursor ion at m/z 253 (ln(A109/A253)), following the order of meta<ortho<para. In addition, the relationships between ln(A109/A253) and collision energy were linear with high correlation coefficients. Comparing the ln(A109/A253) plots of the FUBINAE isomers versus collision energy with similar plots of AB-FUBINACA and its o- and m-fluorobenzyl isomers showed that the three AB-FUBINACA isomers behaved as the FUBINAE isomers did with the same fluorine substitution pattern on the phenyl ring. Moreover, other synthetic cannabinoids with a p-fluorobenzyl group (ADB-FUBINACA, FUB-AMB, FUB-APINACA, FUB-NPB-22, and FU-PX-2) also exhibited behavior similar to p-FUBINAE. These results indicated that the fluorine substitution position on the phenyl ring can be differentiated by collating the model compounds according to the logarithmic plots of their mass spectral abundance ratios as a function of the collision energy.

著者

松田 駿太朗 掛橋 秀直 中野 史保子 志摩 典明 鎌田 徹 西岡 裕 三木 昭宏 坂本 雄紀 宮川 治彦 草野 麻衣子 財津 桂 土橋 均 片木 宗弘

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.22, no.2, pp.109-121, 2017 (Released:2017-07-27)

参考文献数

13

被引用文献数

3

---

In this study, we describe a rapid gas chromatography-tandem mass spectrometry (GC-MS/MS) analytical method that allows comprehensive detection and structural elucidation of synthetic cathinone-type designer drugs. Our proposed method consists of three simultaneous analytical procedures: 1) selective detection of the carbonyl group characteristic to each cathinone examined via selected reaction monitoring (SRM); and the determination of both 2) iminium cations and 3) substituted benzoyl cations generated via the α-cleavage of their corresponding amines and ketone moieties via product ion scanning, respectively.  One peak was detected in the SRM chromatogram for all cathinones examined in procedure 1), as well as the relevant single peaks in the total ion current chromatograms that resulted from procedures 2) and 3) at the same retention time. SRM of procedure 1) showed the transition of substituted benzoyl cations to substituted phenyl cations due to CO elimination, revealing the presence of carbonyl groups within the structures. Each product ion spectrum of the substituted benzoyl cation allowed for both determination of which group was substituted on the aromatic ring and differentiation between corresponding positional isomers for ethyl, methoxy and methylenedioxy substitution. However, identification of the substitution positions for the methyl, bromine and fluorine groups on the aromatic ring was difficult. On the other hand, differences between structural isomers in the product ion spectra of iminium cations were clearly identifiable, allowing for easy discrimination between isomers.

著者

春田 祐輔 森田 敦 大槻 光彦 中園 陽子 中山 秀幸 八ヶ代 一郎 内川 貴志

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.22, no.2, pp.123-132, 2017 (Released:2017-07-27)

参考文献数

12

---

Several synthetic cannabinoids such as AM-2201 contain the 3-carbonyl-N-fluoropentylindole structure. This structure has fluorine positional isomers on the alkyl chain. In most cases, legal controls are placed only on the 5-fluoro analogue. Thus, differentiation of isomers is a significant issue in forensic science. In this study, we developed a method for the differentiation of positional isomers of 3-carbonyl-N-fluoropentylindole derivatives utilizing multiple-stage mass spectrometry using an ion trap tandem mass spectrometer. In addition, the analogues whose fluorine atom was replaced with a chlorine atom or hydroxyl group were also examined.  With respect to each positional isomer of fluorine and chlorine, the ion at m/z 232 or m/z 248, obtained by MS2 analysis of [M+H]+, were selected as the precursor ions for MS3 analysis. The ion at m/z 232 and m/z 248 corresponded to the 3-carbonyl-N-fluoropentylindole and 3-carbonyl-N-chloropentylindole structures. Furthermore, the ion at m/z 212, corresponding to the de-halogenated fragments of the 3-carbonyl-N-fluoropentylindole- and 3-carbonyl-N-chloropentylindole-structures, was selected as the precursor ion for MS4 analysis. Consequently, combination of these MSn analysis achieved differentiation of all the positional isomers.  With respect to positional isomers with the hydroxyl group, however, the fragment ion at m/z 212 was not observed from the MS3 analysis of m/z 230, which corresponds to the 3-carbonyl-N-fluoropentylindole structure. Therefore, differentiation of each positional isomer was not achieved by MSn analysis.  This method is useful for the differentiation of positional isomers of 3-carbonyl-N-fluoropentylindole and 3-carbonyl-N-chloropentylindole derivatives.

著者

掛橋 秀直 志摩 典明 鎌田 寛恵 松田 駿太朗 中野 史保子 和田 美暁 佐々木 啓子 鎌田 徹 西岡 裕 財津 桂 土橋 均 三木 昭宏 片木 宗弘

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.22, no.2, pp.77-90, 2017 (Released:2017-07-27)

参考文献数

26

被引用文献数

1

---

Three analogues of 1-phenyl-2-(pyrrolidin-1-yl)pentan-1-one (α-PVP), 1-(4-fluorophenyl)-2-(pyrolidin-1-yl)pentan-1-one (4F-α-PVP), 1-(4-methoxyphenyl)-2-(pyrrolidin-1-yl)pentan-1-one (4MeO-α-PVP) and 2-(pyrrolidin-1-yl)-1-(thiophen-2-yl)pentan-1-one (α-PVT), and their metabolites were determined in users' urine by liquid chromatography-tandem mass spectrometry using newly synthesized authentic standards. The identified metabolites indicated that metabolic pathways of three α-PVP analogues include the reduction of the carbonyl group to the corresponding alcohols and the oxidation of the pyrrolidine ring to the corresponding pyrrolidone, and 4MeO-α-PVP and α-PVT have additional metabolic pathways of the O-demethylation and the oxidation of thienyl group respectively. The quantitative analyses of the urinary metabolites suggested that the main metabolic pathways of these α-pyrrolidinophenones (PPs) derivatives could vary largely depending on the aromatic rings or substituent groups on the aromatic ring of PPs.

著者

掛橋 秀直 鎌田 寛恵 石川 亜香里 浅井 龍太郎 新田 篤志 和田 美暁 中野 史保子 松田 駿太朗 佐々木 啓子 志摩 典明 鎌田 徹 西岡 裕 三木 昭宏 片木 宗弘

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.24, no.1, pp.73-78, 2019 (Released:2019-01-31)

参考文献数

6

被引用文献数

2

---

N-tert-Butoxycarbonyl-methamphetamine (t-BOCMA), a tert-butoxycarbonyl (t-BOC) derivative of methamphetamine (MA), which has recently been reported in several countries, was seized for the first time in Japan in 2017. It deprotected easily in an acidic condition to result in an illicit MA, and recently became a newly designated drug of the Pharmaceutical and Medical Device Act. For drug enforcement, the information of its properties was, therefore, strongly demanded. In this study, we synthesized the t-BOCMA standard, acquired various analytical data, and demonstrated its conversion to MA in high yield in the relatively moderate acidic condition (5% HCl methanol solution, 50℃). Also, the stability of t-BOCMA in simulated gastric juice (0.08 M HCl, 37℃) was explored by using GC/MS. As the result, 19% of t-BOCMA remained even after 120 min incubation, and the T1/2 was calculated to be 50 min. These suggest that the orally ingested t-BOCMA would be absorbed into blood in some degree without conversion to MA.

著者

松田 駿太朗 片木 宗弘 西岡 裕 鎌田 寛恵 佐々木 啓子 志摩 典明 鎌田 徹 三木 昭宏 辰野 道昭 財津 桂 坪井 健人 土橋 均 鈴木 廣一

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.19, no.2, pp.77-89, 2014 (Released:2014-07-30)

参考文献数

12

被引用文献数

12 18

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Cathinone-type designer drugs are a newly-encountered drug family that has a β-ketophenethylamine skeleton. Recently, the abuse of these drugs has been increasingly common among young adults, and this has caused a serious social problem in many countries. Many of those drugs have become regulated by the Pharmaceutical Affairs Act, and some of them were later banned by the Narcotics and Psychotropics Control Act in Japan, depending on their structures. In this paper, a total of 98 standards of cathinone-type designer drugs were synthesized, and their EI-mass spectra were acquired by GC/MS, with and without trifluoroacetylation. For their free bases, a major fragment ion formed from the α-cleavage of the amine nitrogen was commonly observed. Also, a small fragment ion generated by the α-cleavage of the carbonyl group, followed by the elimination of CO was detectable. For the analogs having an N-alkyl chain longer than methyl group and/or the alkyl side-chain longer than methyl group, a characteristic ion formed from the α-cleavage of the amine nitrogen, followed by the elimination of the olefin moiety was observed. For the trifluoroacetyl derivatives, the intensity of fragment ion formed from the α-cleavage of carbonyl group significantly increased, while that of the fragment ion generated from the α-cleavage of nitrogen decreased, when compared with those of free bases. Also, the ion at m/z 110 was specifically observed for the cathinone analogs having a methylamino group. Those typical fragmentation patterns revealed by analyzing a series of analogs provide useful information for the characterization of cathinone-type designer drugs.

著者

金森 達之 桑山 健次 辻川 健治 宮口 一 岩田 祐子 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.19, no.2, pp.91-101, 2014 (Released:2014-07-30)

参考文献数

12

被引用文献数

1 1

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The in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) and 2,5-dimethoxy-4-isopropylthiophenethylamine (2C-T-4), new psychoactive drugs, were studied using rats. 2C-T-2 hydrochloride and 2C-T-4 hydrochloride were administered separately to male Sprague-Dawley rats via the oral route (10 mg/kg), and the urinary extracts were analyzed by liquid chromatography/mass spectrometry. Fourteen and ten metabolites for 2C-T-2 and 2C-T-4 were detected in the urinary extracts, respectively. Our results suggested that sulfoxidation, sulfone formation, S-dealkylation followed by S-methylation, N-acetylation and deamination followed by oxidation were the major metabolic pathways of 2C-T-2 and 2C-T-4 in rat.

著者

金森 達之 岩田 祐子 辻川 健治 桑山 健次 山室 匡史 瀬川 尋貴 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.21, no.2, pp.139-147, 2016 (Released:2016-07-23)

参考文献数

16

被引用文献数

1 3

---

Simultaneous analytical methods for 18 compounds of fentanyl and its analogues by thin-layer chromatography (TLC), gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) were developed. In TLC, fentanyl analogues were well separated by using toluene-acetone-28% aq. ammonia (20:10:0.3, by vol.) as a developing solvent. In GC/MS, fentanyl analogues, except for fentanyl and acetyl-α-methylfentanyl, could be separated on the extracted ion chromatograms (EIC) of the characteristic fragment ions of each compound. In LC/MS, fentanyl analogues could be separated on the EICs of the protonated molecule of each compound. All of the fentanyl analogues tested were identified correctly by using the combination of TLC, GC/MS and LC/MS.

著者

阿久津 守 杉江 謙一 斉藤 貢一

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.21, no.1, pp.75-85, 2016 (Released:2016-01-23)

参考文献数

19

被引用文献数

2 4

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In order to prove synthetic cannabinoid abuse, it is necessary to detect intact synthetic cannabinoids or their metabolites from such biological samples as urine or blood. Generally, blood is used as the biological sample because it is usually difficult to detect intact synthetic cannabinoids in urine. Furthermore, a rapid and accurate method for the detection of synthetic cannabinoids in the biological sample is required. Therefore, we examined the applicability of solid-phase dispersive extraction (SPDE)-GC/MS in the rapid detection of intact synthetic cannabinoids in blood. We chose seven synthetic cannabinoids designated as narcotics. To determine the optimum operating conditions for SPDE, we selected Oasis® HLB as the solid-phase material for pre-treatment and filled it with 10 mg into the equipment, and acetone as the eluent. The pre-treatment resulted in 80-100% recovery. Furthermore, the pre-treatment time was significantly reduced in SPDE compared to solid-phase extraction (SPE). In addition, the pre-treatment protected operators from unnecessary exposure, reduced cross-contamination of chemicals, and decreased operation complexity. The limit of detection (S/N>3) of JWH-018, JWH-122, cannabicyclohexanol (CCH), XLR-11, and AM2201 was 2.5 ng/mL, and that of JWH-073 and MAM-2201 was 5 ng/mL. The limit of quantification (S/N>10) of JWH-018, JWH-122, CCH, XLR-11, and AM2201 was 5 ng/mL, and that of JWH-073 and MAM-2201 was 10 ng/mL. The average recoveries of the seven synthetic cannabinoids from pooled serum samples spiked at 25 and 450 ng/mL were 76.9-107.4% (SD: 6.4-10.7%) and 63.1-89.6% (SD: 3.9-8.2%), respectively. (SPDE)-GC/MS was proven to be a useful method for detecting intact synthetic cannabinoids in blood.

著者

根岸 祥子 中園 裕紀子 神田 康司 辻川 健治 桑山 健次 金森 達之 岩田 祐子 宮本 和奈 糟谷 史代 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.19, no.2, pp.111-119, 2014 (Released:2014-07-30)

参考文献数

16

被引用文献数

6 5

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Methylamphetamines (methylamphetamine (Me-AP) and methylmethamphetamine (Me-MA)) have three ringpositional isomers. Among them, 4-Me-AP has been controlled as a designated substance (Shitei-Yakubutsu) in Japan since November 2012. Furthermore, Me-APs are a structural isomer of methamphetamine (MA), controlled as a stimulant. Because of the increasing number of structural isomer of designer drugs encountered in forensic science laboratories, analytical differentiation of structural isomers is a significant issue. In this study, a method for differentiation of methylamphetamines using gas chromatography/mass spectrometry (GC/MS) was developed. DB-1ms and DB-5ms columns could not separate free bases and trifluoroacetyl (TFA) derivatives of methylamphetamines while the columns incompletely separated their trimethylsilyl (TMS) derivatives. On the other hand, the mid-polar DB-17ms column separated free bases, TFA and TMS derivatives of 6 methylamphetamines though peak shapes of the free bases were tailing. The best separation was obtained from the analysis of the TMS derivatives on DB-17ms column. The mass spectra showed a little difference between the 2-, 3-Me-AP and 4-Me-AP after TFA derivatization. Also the structural isomers of Me-AP and MA could be differentiated by their EI mass spectra. The results indicated that differentiation of regioisomeric methylamphetamines could be accomplished well by GC/MS of their TMS derivatives on the mid-polar capillary column.