著者

平井 浩文 一瀬 博文 長井 薫 亀井 一郎

出版者

静岡大学

雑誌

基盤研究(B)

巻号頁・発行日

2015-04-01

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世界中で蜂群崩壊症候群や人類に対する悪影響が危惧されている難分解性ネオニコチノイド系殺虫剤(NEOs)が白色腐朽菌により分解可能であるとともに毒性も除去可能であることが明らかとなった。また、本分解反応にはシトクロムP450が関与していることも突き止めた。さらにNEOsのピリジン環を資化可能な細菌を選抜し、白色腐朽菌との共培養を行ったところ、NEOsを効率的に分解可能であることが示唆された。

著者

上田 恵子 辻森 めぐみ 小谷 真也 千葉 亜希子 増野 和彦 久保 昌一 長井 薫 関谷 敦 河岸 洋和

出版者

天然有機化合物討論会実行委員会

雑誌

天然有機化合物討論会講演要旨集

巻号頁・発行日

vol.50, pp.309-314, 2008

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The importance of the endoplasmic reticulum (ER) in triggering a specific program of cell death has been recently reported. By triggering apoptosis on neural cells, ER-stress is a major cause of neurodegenerating disease such as Alzheimer disease. The demand for new protective substances to the ER-stress-dependent cell death prompted us to screen the protective activity of mushrooms. On the course of the screening, we isolated new hericenones, 3-hydroxy-hericenone F as a protective agent, along with related compounds, hericenone I and J. The structures of hericenones were determined by NMR and MS spectra. The structure of 3-hydroxy hericenone was elucidated as (8-formyl-3-hydroxy-5-methoxy-2-methyl-2-(4'-methyl-2'-oxopent-3'-enyl) chroman-7-yl) methyl palmitate, which had one additional hydroxy group compared to hericenone F. 3-Hydroxy-hericenone F showed the protective activity dose-dependently, however hericenones F, I and J did not have any activity at the concentration of 10μg/mL. Therefore, we suggested that the hydroxy group of 3-hydroxy-hericenone F was important for the protective activity. This is the first report of a new hericenone which has the protective activity against ER stress-dependent cell death. The further study using more hericenones is needed for the understanding of the structure-activity relationship.

著者

鈴木 智大 川口 卓巳 天野 裕子 小林 夕香 森田 達也 長井 薫 新井 信隆 河岸 洋和

出版者

天然有機化合物討論会

雑誌

天然有機化合物討論会講演要旨集

巻号頁・発行日

no.48, pp.325-330, 2006-09-15

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In autumn, 2004, 55 people got poisoned by eating an edible wild mushroom, Pleurocybella porrigens (Sugihiratake in Japanese) and 17 people among them died of acute encephalopathy. We found that mice died by injection of the wter-soluble extracts of the mushroom. We tried the isolation of the toxic principle(s) from the extracts. As a result, a lectin (PPL) and a glycoprotein were purified from the mushroom. PPL is a tetramer of identical subunits without S-S linkage. The lectin specifically bound N-acetylgalactosamine among monosacchrides tested. This lectin showed lethal toxicity against rats. In addition, a glycoprotein was isolated as a lethal toxin against mice and rats. The relation between these substances and acute encephalopathy is now being elucidated.

著者

長井 薫

出版者

食品資材研究会

雑誌

ニューフードインダストリー (ISSN:05470277)

巻号頁・発行日

vol.53, no.2, pp.1-12, 2011-02