著者
Satoshi Iwata Shinya Murata Shi Rong Han Akira Wakana Miyuki Sawata Yoshiyuki Tanaka
出版者
国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会
雑誌
Japanese Journal of Infectious Diseases (ISSN:13446304)
巻号頁・発行日
pp.JJID.2016.299, (Released:2016-12-22)
参考文献数
23
被引用文献数
4

A 9-valent human papillomavirus (HPV 6/11/16/18/31/33/45/52/58) virus-like particle (VLP) vaccine (9vHPV) has been proven highly efficacious in preventing anogenital disease related with vaccine HPV types in a pivotal Phase III study in women aged 16 to 26 years. We report here the results of an open-label phase III study conducted to bridge the findings in women age 16 to 26 years to Japanese girls aged 9 to 15 years. All subjects (n = 100) received a 3-dose regimen of 9vHPV vaccine at day 1, month 2 and month 6. Anti-HPV serologic assays were performed at day 1, month 7, month 12, month 24 and month 30. At month 7 (4 weeks after dose 3), 100% of subjects seroconverted for each vaccine HPV type. Increases in geometric mean titers of anti-HPV 6/11/16/18/31/33/45/52/58 in girls were similar to those in Japanese women aged 16 to 26 years in the pivotal phase III study. Persistence of anti-HPV responses was observed through 2 years after dose 3. In addition, administration of 9vHPV vaccine was generally well tolerated in Japanese girls.
著者
Satoshi Iwata Shinya Murata Shi Rong Han Akira Wakana Miyuki Sawata Yoshiyuki Tanaka
出版者
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
雑誌
Japanese Journal of Infectious Diseases (ISSN:13446304)
巻号頁・発行日
vol.70, no.4, pp.368-373, 2017 (Released:2017-07-24)
参考文献数
23
被引用文献数
3 4

A 9-valent human papillomavirus (HPV 6/11/16/18/31/33/45/52/58) virus-like particle vaccine (9vHPV) has been proven highly efficacious in preventing anogenital diseases related to HPV, in a pivotal phase III study for women aged 16-26 years. Here, we report the results of an open-label phase III study conducted to bridge the gap between the findings in women aged 16-26 years and Japanese girls aged 9-15 years. All subjects (n = 100) received a 3-dose regimen of 9vHPV vaccine on day 1 and at months 2 and 6. Anti-HPV serological assays were performed on day 1 and at months 7, 12, 24, and 30. At month 7 (4 weeks after the third dose), 100% of the subjects exhibited seroconversion for each type of HPV. Increases in geometric mean of the titers for anti-HPV 6/11/16/18/31/33/45/52/58 in the subjects were similar to those in Japanese women aged 16-26 years in a previous phase III study. Persistence of the anti-HPV response was observed for 2 years after administration of the third dose. In addition, administration of the 9vHPV vaccine was generally well-tolerated in Japanese girls.
著者
Tran Duy Vinh Yuichi Yoshida Mitsuo Ooyama Tanjuro Goto Ken-ichiro Yasuba Yoshiyuki Tanaka
出版者
一般社団法人 園芸学会
雑誌
The Horticulture Journal (ISSN:21890102)
巻号頁・発行日
pp.OKD-114, (Released:2017-11-17)

Blossom-end rot (BER) in tomato has been generally reported as a calcium (Ca)-related physiological disorder influenced by cultivar and environmental factors. In our previous works, we found that different fruit-sized cultivars could share a similar threshold value of water-soluble Ca. In addition, seasonal susceptibility to BER was closely related to fruit growth rate. This study aimed to clarify the effect of fruit growth rate as a dominant factor determining the susceptibility in different fruit-sized tomato cultivars. A large-sized cultivar, ‘Momotaro Fight’, and medium-sized ‘Cindy Sweet’, with different susceptibility to BER disorder, were hydroponically grown with modified Hoagland nutrient solutions consisting of a range of Ca:K (potassium) ratios in four cropping seasons. In spring and summer, BER incidence was more than 60 and 10% in ‘Momotaro Fight’ and ‘Cindy Sweet’, respectively, when plants were fed with low Ca. BER was rarely observed when water-soluble Ca exceeded 0.30 μmol·g−1 FW, and the rate of BER incidence increased with a decrease in water-soluble Ca concentration in both cultivars. Fruit growth rate was much more vigorous in ‘Momotaro Fight’ than ‘Cindy Sweet’, especially in summer. It was significantly favored by the increased temperature and solar radiation in both cultivars. The multiple regression analyses detected a significant effect of fruit growth rate on BER incidence, exclusively in ‘Momotaro Fight’. Together with water-soluble Ca, fruit growth rate explained over 50% of the variation of BER incidence. A vigorous rate of fruit growth can play a more important role in decreasing water-soluble Ca in ‘Momotaro Fight’, and result in severe and frequent BER incidence, compared to ‘Cindy Sweet’. Thus the cultivar difference in the susceptibility to BER is likely explained by the difference in the growth rate of young fruit affecting water-soluble Ca in the distal part of tomato fruit.
著者
Yoshiyuki Tanaka Shiho Fukuta Sota Koeda Tanjuro Goto Yuichi Yoshida Ken‑ichiro Yasuba
出版者
一般社団法人 園芸学会
雑誌
The Horticulture Journal (ISSN:21890102)
巻号頁・発行日
pp.OKD-115, (Released:2017-10-28)
被引用文献数
2

Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of putative aminotransferase (pAMT) leads to low-pungency and capsinoid accumulation. Therefore, the pamt allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the Capsicum genus, but the variation in pamt alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper ‘No. 4034’ (C. chinense). ‘No. 4034’ contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing ‘No. 4034’ with other accessions. The results indicated that ‘No. 4034’ possessed a loss-of-function pamt allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as pamt9. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of pAMT specifically decreased among biosynthetic genes tested here in ‘No. 4034’, compared with that of pungent accession. pamt9 will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in pAMT mutants.
著者
Erasmus Kirii Tanjuro Goto Yuichi Yoshida Ken-ichiro Yasuba Yoshiyuki Tanaka
出版者
一般社団法人 園芸学会
雑誌
The Horticulture Journal (ISSN:21890102)
巻号頁・発行日
pp.MI-148, (Released:2016-05-27)
被引用文献数
1 4

Pungency in peppers is due to the presence of the alkaloid capsaicin and its analogues, collectively known as capsaicinoids. These compounds are only produced in the Capsicum genus and function as deterrents to mammals from consuming the pepper fruits. Pungency in pepper is qualitatively controlled by the Pun1 locus, which encodes a putative acyltransferase enzyme. Mutations in the Pun1 gene result in a loss of pungency, and several Pun1 loss-of-function alleles have been identified in sweet peppers to date (pun11–3). However, variations in pun1 alleles have not been completely elucidated. In the present study, we report a new type of loss-of-function pun1 allele, named pun14, in a Japanese sweet pepper cultivar, ‘Nara Murasaki’ (C. annuum). Sequence analysis at the Pun1 locus revealed that this type of Pun1 allele is caused by a single adenine nucleotide insertion in the second exon region. This insertion is unique to ‘Nara Murasaki’ and is not present in wild-type Pun1. This insertion causes a frameshift mutation and a change in the amino acid sequence, resulting in a truncated protein. The results of gene expression analysis indicated that the expression of Pun1 in ‘Nara Murasaki’ was hardly detectable, while the transcripts of this gene were strongly expressed in a pungent cultivar. In a co-segregation test, the pun14 genotype perfectly co-segregated with non-pungency in 103 F2 population plants of a cross between ‘Nara Murasaki’ and a pungent cultivar. ‘Nara Murasaki’ and a DNA marker to distinguish the pun14 allele will be informative for understanding the domestication process of sweet peppers.
著者
Kohji OHTA Yoshiyuki TANAKA Isao KAWATE Toshio TSUJI
出版者
一般社団法人 日本機械学会
雑誌
Journal of Biomechanical Science and Engineering (ISSN:18809863)
巻号頁・発行日
pp.14-00207, (Released:2014-09-16)
参考文献数
16
被引用文献数
1

Human can realize flexible and skillful movements by controlling his/her musculoskeletal system and the interactive force with environments appropriately. However it still exist many uncertain biological motor characteristics in human movements even for a simple task. This paper aims to analyze and evaluate acceleration characteristics of the human hand during quick arm motion. First, the dynamic manipulability of end-point via muscle forces, called Human Muscular Mobility Ellipsoid (HMME), is newly defined. Next, the direction-dependent acceleration characteristic of the human hand motion is examined and analyzed its geometrical properties with an approximation ellipses of measured data. It is also discussed the problem that the conventional measures relative to the performance of end-point acceleration cannot represent the geometrical properties of human generated hand acceleration in good agreement with the measured data. Finally, the merits of HMME compared with the conventional measures is then demonstrated with a set of experimental results and computed results: HMME can be utilized in representing the geometric properties of human hand acceleration.
著者
Yuichi Yoshida Nobuyuki Irie Tran Duy Vinh Mitsuo Ooyama Yoshiyuki Tanaka Ken-ichiro Yasuba Tanjuro Goto
出版者
園芸学会
雑誌
Journal of the Japanese Society for Horticultural Science (ISSN:18823351)
巻号頁・発行日
pp.CH-107, (Released:2014-07-04)
被引用文献数
2 4

To understand the factors affecting the incidence of blossom-end rot (BER), the effect of the Ca/K ratio (4/12–12/4, in me·L–1) in nutrient solutions and Ca concentration in fractions in the distal part of young tomato fruits immediately before BER symptoms appear were investigated for three seasons. The rate of BER incidence increased with a decrease in the Ca/K ratio in the supplied solutions in the summer and spring, but little difference was observed in the winter. Ca concentration was highest in winter and lowest in summer, and the concentration in fractions decreased with a decrease in the Ca/K ratio of the solutions. When the results of all three experiments were pooled, among the fractions, water-soluble Ca concentration was found to have the highest significance in the relationship to BER incidence. The risk of BER incidence in rapidly growing tomato increased to a critical level when water-soluble Ca in the distal part of the fresh fruit decreased to less than 0.20 μmol·g–1 FW. Multiple-regression analysis revealed that the concentration of water-soluble Ca, which is predominantly recovering apoplastic or cytoplasmic Ca2+, and total Ca, which has been translocated during fruit development, are significantly affected by solar radiation and Ca concentration in the supplied solution rather than air temperature.
著者
Sota Koeda Kosuke Sato Kenichi Tomi Yoshiyuki Tanaka Rihito Takisawa Munetaka Hosokawa Motoaki Doi Tetsuya Nakazaki Akira Kitajima
出版者
園芸学会
雑誌
Journal of the Japanese Society for Horticultural Science (ISSN:18823351)
巻号頁・発行日
pp.CH-105, (Released:2014-05-10)
被引用文献数
3 5

‘No.80’ (Capsicum chinense) from the Caribbean is a valuable genetic source from the aspect of its non-pungent and highly aromatic traits. In the present study, the non-pungency, volatile components, and phylogenetic origin of ‘No.80’ were analyzed with another C. chinense cultivar, ‘No.2’ from Brazil, which is also non-pungent but less aromatic. Expressions and deduced amino acid sequences of acyltransferase (Pun1) of ‘No.80’ and ‘No.2’ were normal compared with a pungent cultivar, ‘Habanero’. Insertions of 7-bp and 8-bp resulting in frameshift mutations were found in the coding regions of putative aminotransferase (p-AMT) of ‘No.80’ and ‘No.2’, respectively. Co-segregation of these insertions with the non-pungent phenotypes in F1 and F2 populations obtained from crossing ‘No.80’ or ‘No.2’ with ‘Habanero’ suggested that non-pungency in these cultivars arose from genetic mutations of p-AMT that occurred independently. Moreover, molecular phylogenetic analysis suggested that ‘No.80’, a close relative of ‘No.2’, originates from capsicums migrated from the South American mainland. In addition to pungency, we assessed the volatile components of the highly aromatic ‘No.80’, the less aromatic ‘No.2’, and their F1 hybrid using gas chromatography. ‘No.80’ contained higher levels of aroma-contributing volatiles than ‘No.2’, which correlated with the stronger and weaker aromas of two cultivars. Further, the fruit of F1 progenies emitted a number of volatile compounds between or higher than their corresponding parents. Based on these results, the approaches for breeding highly aromatic non-pungent cultivars are discussed.