著者
藤田 克昌
出版者
The Biophysical Society of Japan General Incorporated Association
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.50, no.4, pp.174-179, 2010-07-25
被引用文献数
4 1

Recent developments in fluorescence microscopy techniques have broken the diffraction limit and achieved the spatial resolution of sub 100 nm range. Saturated excitation (SAX) microscopy and stimulated emission depletion (STED) microscopy utilize saturable optical phenomena seen in laser excitation and stimulate emission of fluorescence molecules to induce strongly nonlinear optical effects for the resolution improvement. Photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) applied photoswitchable fluorescence probes for precise measurement of positions of the fluorescence probes in a sample in a few tens of nanometer scale. This review introduces the principles and the characteristics of those super resolution microscopy techniques with discussing the imaging formation and the resolution limit in conventional microscopy techniques.<br>
著者
土居 洋文
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.32, no.4, pp.216-220, 1992-07-25 (Released:2009-05-25)
参考文献数
23
著者
佐藤 了
出版者
一般社団法人日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.32, no.3, pp.53-55, 1992-03-25
参考文献数
8
著者
岡本 尚
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.15, no.3, pp.113-124, 1975-05-25 (Released:2009-05-25)
参考文献数
40
被引用文献数
1 1

The conception of "electrogenic ion pump", actively separating charges across cell membranes, has been recently developed as a concequence of the recognition of the more intimate relationship between the mechanism generating resting membrane potential and the energy metabolism of the cell than formerly supposed. Existence of the resting potential larger than the equilibrium potential of any major permeable ion across cell membrance supports the above view and is regarded as a safety criterion for electrogenic activity. Another criterion, rapidity of the depolarization caused by the application of inhibitory reagents or conditions for energy metabolism, is examined critically taking several experimental results for instances. The investigations towards the energy sources for electrogenic activity and several hypothetical models for electrogenic ion pump are also reviewed. The ion which is transported across membrane by the electhogenic mechanism seems to be H+ in most plant cells, though in some cases Cl- is a possible candidate. The role of H+ in plant cells as a substitute of Na+ in the case of animal cells is reviewed with special reference to the "co-transport" of organic molecules and also from the view point of origin and evolution of the membrane transport systems.
著者
津下 英明
出版者
一般社団法人日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.43, no.4, pp.168-173, 2003-07-25

モノADPリボシルトランスフェラーゼ群として,細菌のモノADPリボシル化毒素と哺乳類モノADPリボシル化酵素が大きなファミリーを形成している.最近タイプIVの毒素に属するアクチンを標的とするアルギニン特異的なVIP2とla,さらにタイプIIIのRhoをADPリボシル化するC3およびラットART2.2の構造が明らかにされた.本稿ではla-NADH複合体の結晶構造とADPリボシル化の反応機構について解説する.
著者
大畑 健次 小西 慶幸 瀬藤 光利
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.50, no.2, pp.080-083, 2010 (Released:2010-03-30)
参考文献数
15

Imaging mass spectrometry (IMS) visualizes the distribution of a wide range of biomolecules in cells and tissue sections. IMS can analyze a variety of biomolecules including unexpected metabolites, without labels in a non-targeted way. We developed AP-MALDI (atmospheric pressure matrix assisted laser desorption/ionization) which is equipped with an atmospheric pressure ion-source chamber. It allows us to analyze flesh samples with minimal loss of intrinsic water or volatile compounds.