著者
Bo-Yeon LEE Qiang LI Woo-Jin SONG Hyung-Kyu CHAE Kyeong KWEON Jin-Ok AHN Hwa-Young YOUN
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0563, (Released:2018-04-18)
被引用文献数
18

Cytotherapy with mesenchymal stem cells (MSCs) has been studied in many species, and often requires in vitro cell expansion to obtain therapeutic doses of stem cells. Because the characteristics of MSCs, such as self-renewal and multi-lineage differentiation, can be altered by long-term culture, it is important to maintain stemness during cultivation. This study assessed the changes in the characteristics of feline adipose tissue-derived (fAT)-MSCs during in vitro passaging. Stem cells isolated from the adipose tissue of donor cats were cultured for seven sub-passages. Proliferation capacity was analyzed by calculating the cell doubling time and by colorimetric assay. Expression of stem cell-specific markers was evaluated by quantitative reverse transcription (qRT)-PCR and immunophenotyping. Expression of adipogenic and osteogenic differentiation markers was also measured by qRT-PCR. Histochemical staining and measurement of β-galactosidase activity were conducted to detect cellular senescence. The cell proliferation rate decreased significantly at passage 5 (P5). Gene expression levels of pluripotency markers (Sox2, Nanog, and Klf4) and stem cell surface markers (CD9, CD44, CD90, and CD105) decreased during continuous culture; in most assays, statistically significant changes were observed at P5. The ability of cells to undergo adipogenic or osteogenic differentiation was inversely proportional to the number of passages. The proportion of senescent cells increased with the number of passages. These results suggest that repeated passages alter the proliferation and multipotency of fAT-MSCs. In clinical trials, early-passage cells should be used to achieve the maximum therapeutic effect.
著者
Tran Thi Phan LY Thi Lien Khai NGUYEN Thu Tan 秋庭 正人 小笠原 夏絵 篠田 ダビデ OKATANI Alexandre Tomomitsu 林谷 秀樹
出版者
社団法人日本獣医学会
雑誌
The journal of veterinary medical science (ISSN:09167250)
巻号頁・発行日
vol.66, no.8, pp.1011-1014, 2004-08-25
被引用文献数
5 45

2000年7月から10月に,ベトナム・メコンデルタの豚,鶏およびアヒルにおけるサルモネラの保菌状況を調べた結果,豚,鶏およびアヒルから,それぞれ5.2%(23/439),7.9%(24/302)および8.7%(31/357)の割合でサルモネラが分離された.また,分離された80菌株は25血清型に型別され,S.Javiana,S.DerbyおよびS.Weltevredenの割合が高かった.また,S.JavianaとS.Weltevredenが3種の家畜から共通して分離された.これらの結果から,これらのサルモネラはこの地域の家畜に広く分布していることが明らかとなった.
著者
Isao MATSUMOTO Kazuyuki UCHIDA James Kenn CHAMBERS Kazumi NIBE Yu SATO Taku HAMASU Hiroyuki NAKAYAMA
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
vol.79, no.10, pp.1660-1663, 2017 (Released:2017-10-07)
参考文献数
16
被引用文献数
3

A 7-year-old castrated male ferret developed unilateral cervical lymphadenomegaly over a 1-month period. Histological examination revealed proliferation of tumor cells in a diffuse and partially nodular pattern. The tumor cells were predominantly Hodgkin cells and binucleated Reed-Sternberg cells, characterized by abundant, clear, vacuolated cytoplasm, pleomorphic, ovoid nuclei with thick nuclear membranes and distinct nucleoli. Multinucleated cells, resembling lymphocytic and histiocytic (L&H) cells, were also observed. Immunohistochemically, the tumor cells expressed Pax-5, BLA-36 and vimentin. A small population of the tumor cells expressed CD20. This case showed proliferation of Hodgkin/Reed-Sternberg cells in conjunction with L&H cells that were histologically analogous to feline Hodgkin’s-like lymphoma. However, Pax-5 and BLA-36 expression along with rare CD20 expression were consistent with classical Hodgkin’s lymphoma in humans.
著者
Gab-Chol CHOI Md. Mahbubur RAHMAN Hwangmin KIM Sehoon KIM In-Seong JEONG
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0307, (Released:2018-04-16)
被引用文献数
4

The aim of this paper is to report two cases of sternal dislocation (SD) in cats and the long-term outcomes with and without surgery. In a cat with poly-traumatized SD (Case 1), mandibular, radial, and ulnar fractures were corrected first, and the SD was allowed to heal without intervention for 14 months. However, normal healing did not occur and sternal instability remained. Therefore, the SD was corrected surgically, and the cat recovered fully within 4 weeks. In a cat with isolated SD (Case 2), surgery was performed, and normal posture and gait were regained after 5 weeks. Furthermore, in both cases, no postoperative complications were observed during follow-up. Therefore, surgical correction of SD in cats is recommended.
著者
Yun-Hsia HSIAO Charles CHEN Hock Siew HAN Rui KANO
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0680, (Released:2018-04-16)
被引用文献数
24

A 2-year-old, exotic shorthair cat presented with baldness and mild scaling on trunk that was confirmed as Microsporum canis (M. canis) infection by the following methods. Wood’s lamp and trichogram were used to demonstrate fungal elements suggestive of dermatophytosis consistent with M. canis. Dermatophyte test medium (DTM) and polymerase chain reaction (PCR) were used for identification. E-test and broth microdilution test were then utilized to estimate antifungal minimal inhibitory concentrations (MICs) towards ITZ and TRF respectively. The strain was isolated from the patient and revealing TRF MIC >32 μg/ml and ITZ MIC 0.023 μg/ml. Patient was cured of dermatophytosis with systemic ITZ.
著者
Gab-Chol CHOI Md. Mahbubur RAHMAN Hwangmin KIM Sehoon KIM In-Seong JEONG
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
vol.80, no.6, pp.1001-1006, 2018 (Released:2018-06-29)
参考文献数
19
被引用文献数
4

The aim of this paper is to report two cases of sternal dislocation (SD) in cats and the long-term outcomes with and without surgery. In a cat with poly-traumatized SD (Case 1), mandibular, radial, and ulnar fractures were corrected first, and the SD was allowed to heal without intervention for 14 months. However, normal healing did not occur and sternal instability remained. Therefore, the SD was corrected surgically, and the cat recovered fully within 4 weeks. In a cat with isolated SD (Case 2), surgery was performed, and normal posture and gait were regained after 5 weeks. Furthermore, in both cases, no postoperative complications were observed during follow-up. Therefore, surgical correction of SD in cats is recommended.
著者
Yuto SANO Minoru OKAMOTO Youhei OOTSUKA Kazuya MATSUDA Shigeki YUSA Hiroyuki TANIYAMA
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
vol.79, no.3, pp.579-583, 2017 (Released:2017-03-23)
参考文献数
20
被引用文献数
5

A 29-year-old stallion presented with bilateral blindness following the chronic purulent nasal drainage. The mass occupied the right caudal nasal cavity and right paranasal sinuses including maxillary, palatine and sphenoidal sinuses, and the right-side turbinal and paranasal septal bones, and cribriform plate of ethmoid bone were destructively replaced by the mass growth. The right optic nerve was invaded and involved by the mass, and the left optic nerve and optic chiasm were compressed by the mass which was extended and invaded the skull base. Histologically, the optic nerves and optic chiasm were degenerated, and the mass was diagnosed as lymphoma which was morphologically and immunohistochemically classified as a diffuse large B-cell lymphoma. Based on these findings, the cause of the blindness in the stallion was concluded to be due to the degeneration of the optic nerves and chiasm associated with lymphoma occurring in the nasal and paranasal cavities. To the best of our knowledge, this is the first report of the equine blindness with optic nerve degeneration accompanied by lymphoma.
著者
Satoko SUGIMOTO Shingo MAEDA Masaya TSUBOI Kohei SAEKI James K. CHAMBERS Tomohiro YONEZAWA Kenjiro FUKUSHIMA Reina FUJIWARA Kazuyuki UCHIDA Hajime TSUJIMOTO Naoaki MATSUKI Koichi OHNO
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0648, (Released:2018-04-09)
被引用文献数
1

A 6-year 5-month-old spayed female Scottish Fold cat presented with a one-month history of gait abnormalities, increased salivation, and decreased activity. A blood test showed hyperammonemia and increased serum bile acids. Imaging tests revealed multiple shunt vessels indicating acquired portosystemic shunt. Histopathologic analysis of liver biopsy showed features consistent with liver hypoperfusion, such as a barely recognizable portal vein, increased numbers of small arterioles, and diffuse vacuolar degeneration of hepatocytes. These findings supported the diagnosis of primary hypoplasia of the portal vein/microvascular dysplasia, (PHPV/MVD). To our knowledge, this is the first case of feline PHPV/MVD that developed multiple acquired portosystemic shunts and presented with hepatic encephalopathy.
著者
Elham A. HASSAN Marwa H. HASSAN Faisal A. TORAD
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0518, (Released:2018-03-29)
被引用文献数
12

The aim of the study was to correlate the clinical severity of pectus excavatum with its type and degree based on objective radiographic evaluation. Twelve brachycephalic dogs were included. Grading of the clinical severity was done based on a 6-point grading score. Thoracic radiographs were used to calculate the frontosagittal and vertebral indices at the tenth thoracic vertebra and the vertebra overlying the excavatum. Correlation between the clinical severity score and frontosagittal and vertebral indices was evaluated using Pearson’s correlation coefficient. Typical pectus excavatum was recorded in the caudal sternum in seven dogs, with a mean clinical severity score of 1.7 ± 1.4, whereas in five dogs, atypical mid-sternal deviation was recorded with a mean clinical severity score of 3.8 ± 0.7. A strong correlation (r=0.7) was recorded between the clinical severity score and vertebral index in the atypical form, whereas a weak correlation (r=0.02) was recorded in the typical form (P<0.05). The clinical severity and degree of pectus excavatum was poorly correlated (r=0.3) in the typical form of pectus excavatum, whereas it was strongly correlated (r=0.9) in the atypical form. Pectus excavatum in dogs is associated with compressive cardiopulmonary dysfunction, which depends mainly on the site/type of deviation rather than the degree of deviation.
著者
Yanfei DENG Guiting HUANG Lingxiu ZOU Tianying NONG Xiaoling YANG Jiayu CUI Yingming WEI Sufang YANG Deshun SHI
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0556, (Released:2018-03-08)
被引用文献数
14

Amniotic mesenchymal stem cells (AMSCs) from livestock are valuable resources for animal reproduction and veterinary therapeutic. The purpose of this study is to explore a suitable way to isolate and culture the buffalo AMSCs (bAMSCs), and to identify their biological characteristics. Digestion with a combination of trypsin-EDTA and collagenase type I could obtain pure bAMSCs more effectively than trypsin-EDTA or collagenase type I alone. bAMSCs could proliferate steadily in vitro culture and exhibited fibroblastic-like morphology in vortex-shaped colony. bAMSCs were positive for MSC-specific markers CD44, CD90, CD105, CD73, β-integrin (CD29) and CD166, and pluripotent markers OCT4, SOX2, NANOG, REX-1, SSEA-1, SSEA-4 and TRA-1-81, but negative for hematopoietic markers CD34, CD45 and epithelial cells specific marker Cytokeratin 18. In addition, bAMSCs were capable of differentiating into adipogenic, osteogenic, chondrogenic and neural lineages, with expression of FABP4, Ost, ACAN, COL2A1, Nestin, β III-tubulin. Glycogen synthase kinase 3 inhibitor: kenpaullone promoted bAMSCs to differentiate into neural lineage. This study provides an effective protocol to obtain and characterize bAMSCs, which have proven useful as a cell resource for buffalo cell reprogramming studies and transgenic animal production.
著者
Takayuki MINESHIGE Kikumi OGIHARA Junichi KAMIIE Go SUGAHARA James Kenn CHAMBERS Kazuyuki UCHIDA Hiroo MADARAME Kinji SHIROTA
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
vol.80, no.3, pp.473-479, 2018 (Released:2018-03-24)
参考文献数
27
被引用文献数
2

Canine squamous cell carcinoma (SCC) shows highly invasive and locally destructive growth. In animal models and human cancer cases, periostin plays a critical role in the enhancement of cancer growth; however, the mechanism of involvement in canine cancers remains unknown. The aim of this study was to examine the involvement of periostin in the pathophysiology of SCC in dogs. We examined the localization of periostin and periostin-producing cells in 20 SCC and three squamous papilloma specimens. Furthermore, we focused on transforming growth factor (TGF)-β1, which was assumed to be an inducing factor of periostin, using culture cells. By immunohistochemistry, limited periostin expression in the stroma was observed in all squamous papillomas. In SCC, periostin protein diffusely expressed at the tumor invasion front of cancer growth. In situ hybridization revealed that periostin mRNA was expressed in the stromal fibroblasts in SCC. In vitro analysis determined that canine SCC cells expressed significantly higher levels of TGF-β1 mRNA compared with canine keratinocytes. In addition, recombinant TGF-β1 induced secretion of periostin from cultured dermal fibroblasts. These data suggest that periostin produced by stromal fibroblasts may be involved in the pathophysiology of canine SCC. TGF-β1 derived from SCC cells may stimulate fibroblasts to produce periostin.
著者
Yohtaro SATO Nobuaki ARAI Hidemi YASUDA Yasushi MIZOGUCHI
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0703, (Released:2018-03-26)
被引用文献数
1

The objectives of this study were to assess if Clinofibrate (CF) treatment improved lipid metabolism in dogs, and to clarify whether its efficacy is influenced by canine characteristics. We collected medical records of 306 dogs and performed epidemiological analyses. Lipid values of all lipoproteins were significantly decreased by CF medication, especially VLDL triglyceride (TG) concentration (mean reduction rate=54.82%). However, 17.65% of dogs showed drug refractoriness in relation to TG level, and Toy Poodles had a lower CF response than other breeds (OR=5.36, 95% CI=2.07–13.90). Therefore, our study suggests that genetic factors may have an effect on CF response, so genetic studies on lipid metabolism-related genes might be conducted to identify variations in CF efficacy.
著者
Rina NABETA Hironari OSADA Misato OGAWA Ayana HASEGAWA Miori KISHIMOTO Hirotada MURAYAMA Toshinori YOSHIDA Makoto SHIBUTANI Hiroshi ITOH Keitaro OHMORI
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
vol.79, no.9, pp.1489-1493, 2017 (Released:2017-09-12)
参考文献数
10
被引用文献数
2

A 9-year-old, spayed female Chihuahua was presented with a 1-week history of lethargy and anorexia. Abdominal ultrasonography and computed tomography found bilateral adrenal masses without metastasis. Serum cortisol levels that were sampled before and after an adrenocorticotropic hormone stimulation test were within reference ranges. Lethargy and anorexia completely resolved after short-term fluid therapy; the clinical signs did not occur for approximately 8 months until her sudden death. A postmortem examination revealed bilateral adrenocortical carcinomas and liver metastasis. Primary adrenocortical carcinomas developed in the dog met the definition of bilateral incidental adrenal gland masses (IAGMs). This is the first case report to demonstrate based on histological identification that adrenocortical carcinomas cause bilateral IAGMs in dogs.
著者
Ryosuke ECHIGO Atsushi FUJITA Ryohei NISHIMURA Manabu MOCHIZUKI
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0318, (Released:2018-03-22)
被引用文献数
5

The cases of 4 Pomeranians with injury of the triceps brachii tendon that underwent surgical treatment were retrospectively reviewed to evaluate some clinical findings including signalment, cause of injury, clinical signs, pattern of injury, surgical technique, external coaptation after operation, complications, and outcomes. While all of the dogs showed non-weight bearing posture of the affected limbs and severe pain shortly after injury onset, the pain level decreased over time. A characteristic finding of the 4 cases was an absence of tension in the triceps brachii tendon when the elbow joint was flexed. The pattern of triceps brachii tendon injury was either laceration of the central part of the tendon (n=1) or tendon rupture at its insertion to the olecranon (n=3). Although there were no major complications after surgery in 3 cases, the remaining case required a revision surgery. Long lateral splint was effective method for external coaptation after operation. Diagnosis of triceps brachii tendon injury was not difficult if we even recognize this trauma. This form of injury can have a good prognosis with adequate surgery and postoperative coaptation.
著者
Kazuhiro WATANABE Ryota IWASAKI Syun KONDO Tomohiro OHUCHI Sanae SHIBATA Hiroki SAKAI Kazuaki YAMAZOE
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0597, (Released:2018-03-20)
被引用文献数
3

We present a case of an intact 14-year-old male dog with a prostate B-cell lymphoma recognized in the contents of an irreducible perineal hernia. The enlarged prostate was replaced after reducing its size by partial excision, and the perineal hernia was repaired using the tunica vaginalis communis. However, the pelvic cavity was largely occupied by the replaced prostate, and urinary retention developed. The prostate was resected on the next day via abdominal median incision, and a bladder-urethra anastomosis was performed. Lymphoma has rarely been demonstrated to develop in the prostate, with the lesion comprising the hernia contents. The present study suggests the necessity of early treatment for perineal hernias and the possibility that lymphoma may be present in the hernia contents.
著者
Tatsushi MORITA Yutaka MOMOTA Akihiro MORI Hitomi ODA Kazunori IKE Toshinori SAKO
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.17-0274, (Released:2018-03-08)
被引用文献数
5

A 12-year-old female Shih-Tzu with hyperadrenocorticism and hypothyroidism developed concurrent refractory generalized demodicosis that did not respond to doramectin treatment. Although amitraz treatment was effective, the dog developed severe diabetes, which resulted in the cessation of amitraz and trilostane. Attempts to control the diabetes were unsuccessful, and its hyperadrenocorticism was left untreated, leading to the recurrence of demodicosis. However, demodicosis went into complete remission with a single dose of fluralaner. Transient erythematous papules appeared on the trunk three days after the administration of fluralaner, but no other adverse reactions were noted. We demonstrated that fluralaner is a potent treatment for demodicosis, and skin eruptions are possible after the first dose of the drug.
著者
武内 ゆかり 森 裕司
出版者
社団法人日本獣医学会
雑誌
The journal of veterinary medical science (ISSN:09167250)
巻号頁・発行日
vol.68, no.8, pp.789-796, 2006-08-25
参考文献数
21
被引用文献数
3 50

日本で飼育されている純血種犬56品種の行動特性を明らかにする目的で,96名の小動物臨床獣医師に村してアンケート調査を行った.方法は米英国における先行研究に準じた.その結果,米国と同じく,13項目全ての行動特性について有意な品種差が検出された.また,他犬に対する攻撃性,縄張防衛,興奮性,一般的な活動性,飼い主に村する攻撃性,破壊性,警戒咆哮,子供に対する攻撃性については雄の方が,また服従性とトイレのしつけについては雌の方が高いと有意に多くの獣医師が感じていることが明らかとなった.遊び好き,無駄吠え,愛情要求については,性差がないと考えている獣医師が有意に多かった.この結果についても米英国での調査と比較すると傾向はほぼ一致した.さらに調査データを因子分析により解析した結果,米英国のデータより1つ少ないものの,共通する3つの因子(攻撃性,反応性,訓練能)が抽出された.また米国のデータと比較するために,これら3つの因子得点をもとにクラスター分析を行い,全犬種を7クラスターに分類したところ,米国と共通して調査された38犬種のうち22犬種が同一クラスターに分類された.これらの結果より,地域的・文化的背景が異なるにもかかわらず,日本においても米英国と同じように純血犬種や性別によって行動特性の異なることが示唆され,多くの犬種についてその行動特性が類似していることが明らかとなった.
著者
湯山 輝彦 遊佐 繁基 吉住 和親 山野 茂樹 村田 聡一郎 廣瀬 友亮 長内 利佳 大西 陽子 大里 聡 佐々木 千枝 佐々木 由香子 角田 勤 椿 志郎 高井 伸二
出版者
社団法人日本獣医学会
雑誌
The journal of veterinary medical science (ISSN:09167250)
巻号頁・発行日
vol.64, no.8, pp.715-718, s・iii-s・iv, 2002-08-25
参考文献数
22
被引用文献数
4 9

鹿児島県の5カ所の軽種馬生産牧場で生産された13頭のサラブレッド種の仔馬と母馬における15-17kDa抗原(VapA)陽性のRhodococcus equi強毒株の分離頻度を検討し,制限酵素切断像による病原性プラスミド型別を行い,これまでに報告された日本のプラスミド型と比較した.13頭の仔馬糞便から分離した886株中218株(24.6%)が,親馬から分離した104株中13株(12.5%)が強毒株であった.231の強毒株のプラスミドDNAを制限酵素EcoRI,EcoT22I及びHindIIIで検索したところ,172株が90-kb type I型,57株が90-kb type II型,2株が90-kb type IV型の病原性プラスミドを保有し,3種類のプラスミドに型別された.以上の成績から,鹿児島の軽種馬から分離された強毒株が保有する病原性プラスミドの分布に地域特異性のあることが明らかとなった.
著者
Junwoo BAE Mahmoud SOLIMAN Hyunwoo KIM Seongwoo KANG Woosun KIM Soomin AHN Kyoungoh CHO Jihye CHOI Suhee KIM Jinho PARK Sangki KIM Yoonjung DO Jaegyu YOO Dohyeon YU
出版者
JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
vol.79, no.9, pp.1591-1595, 2017 (Released:2017-09-29)
参考文献数
24
被引用文献数
7

Hydroxyethyl starches (HES) are commonly used synthetic colloidal solution in veterinary medicine. Despite of possible adverse effect to kidney injury in human, there is no report about nephrotoxic effects of HES in dogs. HES was administered to a Golden retriever (4-year-old, intact male) with ascites in order to increase plasma osmolality. Initially, the dog was mild azotemic, however, kidney function was rapidly deteriorated after several days of HES administration. Finally, histopathological examination revealed remarkable osmotic nephrosis. In the case reported herein, acute kidney injury was remarkably developed after HES administration. Clinical and histopathologic findings of acute kidney injury support nephrotoxic effects of HES to a dog.