著者

Nagi TOMITA Yuji SUNDEN Yoshiharu OKAMOTO Takehito MORITA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0168, (Released:2017-06-11)

被引用文献数

3

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A13-year-old spayed female dog had a mass in the left auricle. Grossly, connection between the mass and original auricular cartilage was not recognized. The mass was unencapsulated and contained multiple islands of mature hyaline cartilage and neoplastic adipocytes. The neoplastic cells comprised predominant mature adipocytes, scattered lipoblasts and irregular round to spindle cells with moderate atypia. The atypical cells occasionally had lipid droplets. A diagnosis of well-differentiated liposarcoma (WDL) with chondroid metaplasia was made. This is the first report for liposarcoma with chondroid metaplasia in the auricle of domestic animals.

著者

Harumichi ITOH Shimpei NISHIKAWA Tomoya HARAGUCHI Yu ARIKAWA Shotaro ETOH Toshie ISERI Kenji TANI Munekazu NAKAICHI Yasuho TAURA Kazuhito ITAMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0503, (Released:2017-06-05)

被引用文献数

5

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Adipose-derived stem cells (ADSCs) are abundant and readily obtained, and have been studied for their clinical applicability in regenerative medicine. Some surface antigens have been identified as markers of different ADSC subpopulations in mice and humans. However, it is unclear whether functionally distinct subpopulations exist in dogs. To address this issue, we evaluated aldehyde dehydrogenase (ALDH) activity—a widely used stem cell marker in mice and humans—by flow cytometry. Approximately 20% of bulk ADSCs showed high ALDH activity. Compared to cells with low activity (ALDHLo), the high-activity (ALDHHi) subpopulation exhibited a higher capacity for adipogenic and osteogenic differentiation. This is the first report of distinct ADSC subpopulations in dogs that differ in terms of adipogenic and osteogenic differentiation potential.

著者

Chul-Song PARK Gil-Jae CHO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0563, (Released:2017-04-20)

被引用文献数

3

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The objective of this study was to determine the number of Eurasian otters (Lutra lutra) that occupied the Sincheon River in Daegu, South Korea. Twenty-seven spraints collected from February to May 2016 at four sites (Jangam Bridge approximately 6.1 km from the Gachang Dam, Docheong Bridge approximately 13.5 km, Chimsan Bridge approximately 15.1 km and Nogoek Bridge approximately 18 km) along the Sincheon River (approximately 27.06 km) were analyzed using 12 microsatellite markers. The analyses resulted in the identification of 16 (59.3%) individual Eurasian otters in the Sincheon River based on the 27 spraints. Of the 16 individual Eurasian otters, seven were male, and nine were female. Groups were centered at the Jangam Bridge (3 males and 2 females), Chimsan Bridge (2 males and 3 females) and Docheong Bridge (2 males and 4 females). Thus, the 16 Eurasian otters formed three genetically related groups in each sampling area. The number of alleles per locus varied from three to seven, with a mean value of 5.08 alleles.

著者

村上 洋介 加藤 あずさ 津田 知幸 両角 徹雄 三浦 康男 杉村 崇明

出版者

社団法人日本獣医学会

雑誌

日本獣医学雑誌 (ISSN:09167250)

巻号頁・発行日

vol.56, no.5, pp.891-894, 1994-10-15

被引用文献数

37

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わが国で発生した肥育豚の慢性肺炎と繁殖豚の異常産の各症例から, 豚の肺胞マクロファージ細胞を用いて合計3株の豚生殖器・呼吸器症候群(PRRS)ウイルスを分離した. これらの国内分離株は, 間接蛍光抗体法(IIF)とIndirect immunoperoxidase mono-layer assayのいずれにおいてもアメリカで分離されたPRRSウイルスの46448株(米国株)とは強い交差反応を示すが, オランダで分離されたLelystad virus (欧州株)とは弱い交差反応しか示さなかった. さらに, 自然感染豚の血清を用いて, 上記の米国株, 欧州株および国内分離株に対するIIF抗体の検出率と抗体価の変動を比較したところ, 国内分離株と米国株を抗原とした場合にはそれらの抗体検出率と抗体価の変動がほほ一致するのに対して, 欧州株を抗原とした場合には抗体検出率が低く, また抗体変動もほとんど認められなかった. 以上のことから, わが国でPRRSウイルスを分離し国内にPRRSの発生があることを明らかにするとともに, 国内流行株が米国株に近縁であることが示唆された.

著者

Eugene C. BWALYA Sangho KIM Jing FANG H.M. Suranji WIJEKOON Kenji HOSOYA Masahiro OKUMURA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0084, (Released:2017-05-27)

被引用文献数

11

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Mesenchymal stem cells (MSC) are a potential alternative source of differentiated chondrocytes for cartilage tissue regeneration and repair of osteoarthritic (OA) joints. We investigated the effects of pentosan polysulfate (PPS) and polysulfated glycosaminoglycan (PSGAG) on chondrogenesis of canine bone marrow-derived mesenchymal stem cells (cBMSC) in alginate and micromass cultures (MMC). Chondrogenic differentiation medium (CDM) was supplemented with PPS or PSGAG at concentrations of 0 (positive control; PC), 1, 3 and 5 μg/ml. 10% DMEM was used as negative control. Chondrocyte phenotype was analyzed by quantitative real-time PCR (qPCR) for alginate cultures and Alcian blue staining for proteoglycan (PG) synthesis for MMC. In alginate culture, PPS and PSGAG showed no significant effect on type II collagen, aggrecan and HIF-2α mRNA expression. PPS had no significant effect on type I collagen whereas PSGAG significantly upregulated (P<0.05) it at all concentrations relative to other treatments. PPS demonstrated a dose-dependent inhibitory effect on type X collagen mRNA with significant inhibition observed at 5 μg/ml compared to the NC. PSGAG showed an inverse effect on type X collagen with 1 μg/ml significantly inhibiting its expression while increase in the concentration correspondingly increased type X collagen expression. In MMC, PPS significantly enhanced chondrogenesis and PG deposition whereas PSGAG inhibited chondrogenesis and promoted a fibrocartilage-like phenotype with reduced PG deposition. While PPS enhances chondrogenesis of cBMSC in MMC, the response of MSC to chondroinductive factors is culture system-dependent and varies significantly between alginate and MMC.

著者

Dah-Renn FU Daiki KATO Yoshifumi ENDO Tsuyoshi KADOSAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.78, no.7, pp.1161-1166, 2016 (Released:2016-08-01)

参考文献数

38

被引用文献数

10

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Nasal lymphoma is the most common nasal tumor in cats and is generally a solitary and radiosensitive tumor. We retrospectively evaluated the response to radiation and survival time in relation to apoptosis and Ki-67 indices in feline nasal lymphomas treated with radiation therapy. The apoptotic and Ki-67 indices were evaluated with TUNEL and immunohistochemical staining in 30 biopsy tissues that were taken before any treatment. These two indices were compared, and differences between different treatment response groups were analyzed. The correlation between the median survival times (MST) and the indices was estimated using the Kaplan Meier method, and statistical differences between survival curves were analyzed using a log-rank method. With regard to apoptotic index, a statistical difference was observed between the samples taken from cats with complete response and stable disease (1.22% vs. 0.45%; P=0.045). The Ki-67 index in cats with both complete response and partial response was significantly higher than in cats with stable disease (44.4% and 39.6% vs. 16.3%; P<0.001 and P=0.008, respectively). The cats with a high level of apoptosis (>0.9%) nasal lymphoma were not significantly prolonged MSTs (P=0.202), however, high Ki-67-positive (>40%) cats experienced a statistically significant relationship with longer survival time (P=0.015). Our results indicate that spontaneous apoptotic and Ki-67 indices are strong predictors for response to radiation therapy in feline nasal lymphomas.

著者

Yoon-Joo SHIN Nam-Shik SHIN

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0403, (Released:2017-05-25)

被引用文献数

7

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Sociability is an essential trait for dogs to successfully interact with humans. In this study, the relationship between sociability and physiological stress was examined. Additionally, whether differences exist between companion dogs (C group) and shelter dogs (S group) was examined. Overall, healthy 37 dogs (C group=21 and S group=16) were examined. After 5 min of walking, the dog and the owner (or the chief manager) rested freely in the experimental location for 5 min. The behavioral test with 6 categories was conducted to evaluate sociability over 4 min. The establishment of two groups (H group=dogs with high sociability; L group=dogs with low sociability) was supported by the statistical results of the behavioral tests. Saliva was collected before (P1) and after the test period (P2), and salivary cortisol levels were determined and statistically analyzed. The cortisol concentrations at P2 and the differences in concentrations between P1 and P2 (P2–P1) in the groups with high sociability were significantly lower than those in the groups with low sociability. These results may demonstrate that sociable dogs adapt more comfortably to strangers and unfamiliar situations. Meanwhile, there were significant differences in hormonal results between the C and S groups. For this reason, their sociability should be evaluated using behavioral and physiological assessments before re-adoption to ensure their successful adaptation.

著者

Manabu KOIKE Yasutomo YUTOKU Aki KOIKE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.3, pp.554-561, 2017 (Released:2017-03-23)

参考文献数

32

被引用文献数

11

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Understanding the molecular mechanisms of DNA double-strand break (DSB) repair machinery, specifically non-homologous DNA-end joining (NHEJ), is crucial for developing next-generation radiotherapies and common chemotherapeutics for human and animal cancers. The localization, protein-protein interactions and post-translational modifications of core NHEJ factors, might play vital roles for regulation of NHEJ activity. The human Ku heterodimer (Ku70/Ku80) is a core NHEJ factor in the NHEJ pathway and is involved in sensing of DSBs. Companion animals, such as canines, have been proposed to be an excellent model for cancer research, including development of chemotherapeutics. However, the post-translational modifications, localization and complex formation of canine Ku70 have not been clarified. Here, we show that canine Ku70 localizes in the nuclei of interphase cells and that it is recruited quickly at laser-microirradiated DSB sites. Structurally, two DNA-PK phosphorylation sites (S6 and S51), an ubiquitination site (K114), two canonical sumoylation consensus motifs, a CDK phosphorylation motif, and a nuclear localization signal (NLS) in the human Ku70 are evolutionarily conserved in canine and mouse species, while the acetylation sites in human Ku70 are partially conserved. Intriguingly, the primary candidate nucleophile (K31) required for 5’dRP/AP lyase activity of human and mouse Ku70 is not conserved in canines, suggesting that canine Ku does not possess this activity. Our findings provide insights into the molecular mechanisms of Ku-dependent NHEJ in a canine model and form a platform for the development of next-generation common chemotherapeutics for human and animal cancers.

著者

Mizuki KURAMOCHI Takeshi IZAWA Shin NISHIMURA Terumasa SHIMADA Mitsuru KUWAMURA Jyoji YAMATE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0117, (Released:2017-05-20)

被引用文献数

1

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An 11-year-old female golden retriever dog had a mass at the right corner of the upper lip, which gradually increased in size and protruded into the oral cavity. The mass was removed surgically. The cut surface of the mass was smooth, whitish and solid, and covered by the oral mucosal membrane. Histopathologically, the mass consisted mainly of reticular pattern of short spindle cells that stained positively for cytokeratin AE1/AE3, α-smooth muscle actin and p63, suggestive of a myoepithelial cell phenotype. Between the neoplastic cords, there was myxoid or edematous connective tissue. Additionally, neoplastic cells with luminal epithelial and basal cell phenotypes were arranged in ducts and small islands, respectively. Based on the diverse histological and immunohistochemical features, the tumor was diagnosed as pleomorphic adenoma of the labial gland. To our knowledge, the reticular proliferation pattern of myoepithelial cells has not been described in salivary gland tumors of domestic animals.

著者

Andrea CANTALAMESSA Stefano MARTIN Andrea MARCHEGIANI Alessandro FRUGANTI Fabrizio DINI Adolfo Maria TAMBELLA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0281, (Released:2017-05-18)

被引用文献数

3

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A 4-year-old intact female, mixed breed dog was presented with a complaint of dyspnea. Clinical examination revealed symptoms related to disease of the upper airways. Radiographic findings were consistent with tracheal collapse associated with anomalies involving the seventh cervical vertebra and the first ribs bilaterally. Radiographs were highly suggestive of cervical ribs; computed tomography and ultrasound examination allowed complete characterization and better localization of the anomalies with relationship to the adjacent muscle and vasculature. Cervical ribs are malformations widely described in human medicine, but only sporadically in dogs. Herein, we discuss etiological, clinical, diagnostic, and therapeutic aspects of cervical ribs and possible correlations between the cervical ribs and other anatomical anomalies noted in this dog.

著者

Jaehwan KIM Kidong EOM Hakyoung YOON

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0031, (Released:2017-05-11)

被引用文献数

2

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A 14-year-old dog weighing 4 kg presented with hypotension only in the right forelimb. Thoracic radiography revealed a round soft tissue opacity near the aortic arch and below the second thoracic vertebra on a lateral view. Three-dimensional computed tomography angiography clearly revealed stenosis and aneurysmal dilation of an aberrant right subclavian artery. Stenosis and aneurysm of an aberrant subclavian artery should be included as a differential diagnosis in dogs showing a round soft tissue opacity near the aortic arch and below the thoracic vertebra on the lateral thoracic radiograph.

著者

Nobuyuki KANNO Tomohiro SUZUKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0018, (Released:2017-05-01)

被引用文献数

3

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Sick sinus syndrome (SSS) is a type of bradyarrhythmia that can lead to syncope. Cilostazol has been reported to be an effective treatment for human patients with SSS and other bradyarrhythmias. This report describes the successful long-term treatment with cilostazol in a dog with SSS. A nine-year old intact male Miniature Schnauzer presented with a history of syncopal episodes and unsteady gait. After cilostazol treatment, the total heart rate (HR), mean HR, and frequency of premature ventricular contractions (PVCs) increased, while the maximum HR and maximum pause time decreased. Additionally, the number of syncopal episodes decreased. The dog died suddenly, 1418 days after the start of cilostazol treatment. Cilostazol may be a useful therapeutic agent in canines with SSS.

著者

Mun Keong KOK James K. CHAMBERS Atsushi DOHATA Kazuyuki UCHIDA Ryohei NISHIMURA Hiroyuki NAKAYAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0178, (Released:2017-05-01)

被引用文献数

4

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A 10-year-old mixed breed dog was presented with a 0.8 cm diameter mass below the left eye region. The mass was surgically removed and processed for histopathological examination. Microscopically, tumor cells proliferated in small lobules, nests and cords, and the tumor parenchyma was separated by desmoplastic stroma. Majority of the tumor cells were periodic acid-Schiff (PAS)-positive, and the desmoplastic stroma was densely collagenous and mucinous. Immunohistochemical results showed that the tumor cells were diffusely positive for cytokeratin 15, cytokeratin 19 and CD 34, while cytokeratin 8 reactivity was limited to the tumor cells proliferating in cords. Few tumor cells were positive for nestin. Based on the histopathological findings, the tumor was diagnosed as desmoplastic tricholemmoma.

著者

Mao JIKUYA Morihiro TATENO Masashi TAKAHASHI Yasuyuki ENDO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0546, (Released:2017-05-08)

被引用文献数

1

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The Iriomote cat and Tsushima leopard cat are endangered wildcats in Japan and inhabit only Iriomote-jima and Tsushima islands, respectively. Domestic dogs and cats living on Iriomote-jima and Tsushima islands were surveyed to clarify the interrelationship between wildcats and domestic animals regarding tick-borne disease transmission. Pathogen-derived DNA in blood samples was detected by polymerase chain reaction. Babesia gibsoni was detected in dogs of Iriomote-jima, and Hepatozoon felis and hemoplasmas were detected in domestic cats of Tsushima. Because the H. felis detected in this study was closely related to that isolated from wildcats, we suspect that common H. felis is harbored and transmitted among wildcats and domestic cats via ticks in Tsushima.

著者

Kohei NAKATA Harumi MIURA Hiroki SAKAI Takashi MORI Sanae SHIBATA Hidetaka NISHIDA Sadatoshi MAEDA Hiroaki KAMISHINA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0142, (Released:2017-05-04)

被引用文献数

10

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A 7-year-old cat was referred with pelvic limb ataxia. Radiography and CT revealed bone resorption of the L1 vertebral arch, and myelography identified a compressive extradural lesion. The mass was surgically removed and histopathologically diagnosed as giant cell osteosarcoma. Three years later, the recurrent tumor resection and vertebral fixation were performed. Six months later, vertebrectomy was performed to radically excise the recurrent mass and a titanium spinal cage was placed. The cat is alive approximately 5 years after the first surgery. This case report describes vertebrectomy and vertebral body replacement as a radical treatment for feline vertebral osteosarcoma.

著者

Syakalima Michelo CHOONGO Kennedy 中里 幸和 小沼 操 杉本 千尋 坪田 敏男 福士 秀人 吉田 光敏 板垣 匡 安田 準

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.63, no.3, pp.315-318, 2001-03-25

被引用文献数

9

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ザンビア国カフエ川湿原で, 銅鉱山排液が混入するロッキンバー及びブルーラグーン国立公園に生息する野生生物, 重金属被爆がもたらす環境危険因子について食物連鎖解析を行った.ここでみられる食物連鎖因子は, 水, 魚, 植物(草).カフエレチュエ(Kobus Leche Kafuensis)であり, 重金属は, 銅, 亜鉛, マンガン, 鉄である.銅は水:0.03-0.04, 魚3.0-6.0, 草11.0-44.0, レチュエ肝臓:痕跡-199.0であった.亜鉛は水:0.01, 魚32.0-82.0, 草:15.0-21.0.レチュエ肝臓:52.0-138.0であった.マンガンは水:0.15-0.16, 魚:7.0-18.0, 草:51.0-1450, レチュエ肝臓:40.0-53.0であった.鉄は水:0.13-0.14, 魚:26.0-134.0, 草:1766.0-1797.0, レチュエ肝臓:131.0-856.0であった.濃度単位は水がmg/l, その他の試料はmg/kgである.水以外の全ての因子で重金属濃度が高かったが, 毒性は裏づけられなかった.

著者

Angeline Ping Ping TEH Watanyoo PRATAKPIRIYA Yuichi HIDAKA Hiroyuki SATO Takuya HIRAI Ryoji YAMAGUCHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.4, pp.714-718, 2017 (Released:2017-04-05)

参考文献数

17

被引用文献数

4

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A 3.5-year-old female Chihuahua was presented with complaint of neck pain, intermittent cough and dysphagia. Physical examination and diagnostic imaging of neck region revealed a solid and highly vascularized mass involving the retropharyngeal region. Histologically, the mass showed an atypical zellballen pattern which comprised of high density of type I chief cells with high nuclear cytoplasmic ratio and separated by delicate fibrovascular stroma. Immunoreactivity for neuroendocrine markers was diffusely positive in cytoplasm of tumor cells. Disseminated tumor emboli in external jugular vein were detected 6 months after initial surgery. An electron microscopic study revealed numerous electron-dense intracytoplasmic neurosecretory granules. Based on these findings, carotid body carcinoma was diagnosed.

著者

高瀬 公三 馬場 守ジルベルト 西 理佳子 藤川 英雄 山田 進二

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.57, no.2, pp.327-330, 1995

被引用文献数

1

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発育鶏卵漿尿膜(CAM)及び鶏腎培養細胞(CK)由来の鶏アデノウイルス(FAV)寒天ゲル内沈降(AGP)抗原の反応性を実験感染鶏血清及び野外鶏血清を用いて比較し, 次の成績を得た. 実験感染鶏血清の両由来抗原による陽性率はFAVの株(血清型)の違いによって異なり, ヘテロ抗原での陽性率はホモ抗原でのそれに比べて低く, またヘテロ抗原での抗体価もホモ抗原の抗体価に比べて低い傾向を示した. さらに, CAM由来抗原を用いた方がCK由来抗原の場合よりも高い陽性率, 又は高い抗体価の得られる場合が多かった. CAM由来抗原を用いて野外鶏血清を検査したところ, 用いる抗原(血清型)によって抗体陽性率は異なったが, 複数の抗原を混合することにより, 陽性率は高まった. このことから, FAVの血清疫学調査にはCAM由来の複数の血清型のAGP抗原を用いて行うことが望ましいと考えられた.

著者

堀本 泰介 福田 奈保 岩附(堀本) 研子 GUAN Yi LIM Wilina PEIRIS Malik 杉井 俊二 小田切 孝人 田代 眞人 河岡 義裕

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.66, no.3, pp.303-305, 2004-03-25

被引用文献数

1

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1997年に人から分離されたH5N1インフルエンザウイルスの赤血球凝集素(HA)に対する単クローン性抗体を,DNA免疫法を利用して作製した.これらの抗体を用いて,H5型ウイルスのHA抗原性を解析したところ,1997年および2003年に人から分離されたH5N1ウイルスの間で抗原性がかなり異なることがわかった.

著者

高瀬 公三 馬場 守 ジルベルト 有吉 理佳子 藤川 英雄

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.58, no.11, pp.1129-1131, 1996-11-25

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SPF発育鶏胚の高度病原性伝染性ファブリキウス嚢病ウイルス(hv IBDV)F539株及びDV86株に対する感受性を, 3つの接種ルートで, 従来型IBDV G691株と比較しながら調べた. その結果, 鶏胚のhv IBDV 2株に対する感受性はG691株より高く, 感染胚のほぼ100%が死亡し, 算出された50%胚致死量は50%胚感染量とほぼ同値を示した. 鶏胚に対する高い致死性はhv IBDVに特徴的な性状と思われた.