著者

Masanori KOBAYASHI Tatsuya HORI Eiichi KAWAKAMI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0108, (Released:2018-06-22)

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Two dogs with low plasma testosterone (T) levels and poor semen quality were administered one tablet of 12.5 mg clomiphene citrate orally per day at 2-day intervals for 4 weeks. Plasma T levels, total sperm count, and sperm motility in both dogs temporarily increased between 3 and 6 weeks after the start of treatment. These results indicate that poor semen quality in dogs with low plasma T level can be improved by oral administration of clomiphene citrate.

著者

Daneeya EKAPOPPHAN Athicha SRISUTTHAKARN Walasinee MOONARMART Ruangrat BUDDHIRONGAWATR Norasuthi BANGPHOOMI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0045, (Released:2018-06-20)

被引用文献数

20

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This study aims to determine the microbiological profile and risk factors associated with antimicrobial-resistant bacteria in canine severe corneal ulcers. Thirty-two corneal and conjunctival swabs were collected from dogs with diagnosed severe corneal ulcers that presented to Prasu-Arthorn veterinary teaching hospital in Nakhon Pathom, Thailand from June 2015 to June 2016. Microorganisms were identified by means of genotypic and phenotypic approaches. Of 32 ulcers sampled, 26 (81.3%) yielded culturable microorganisms with 24 bacterial isolates and 7 fungal isolates. The most commonly isolated bacteria were Staphylococcus spp. (45.8%, 11/24) and Pseudomonas aeruginosa (20.8%, 5/24). Out of 11 staphylococcal isolates identified, 10 carried the mecA gene providing methicillin resistance. The extended-spectrum β-lactamase (ESBL) encoding genes blaCTX-M and blaVEB-1were found in an Acinetobacter lwoffii isolate, and blaSHV was found in a P. aeruginosa isolate. Based on the Clinical Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoint criteria, minimum inhibitory concentrations values showed that all bacteria, except for staphylococci, were susceptible to current ophthalmic antibiotics. More than 50% of staphylococci were resistant to all generations of fluoroquinolones and fusidic acid. Chloramphenicol was highly active against staphylococci (81.3% susceptible). The width (P=0.02) and the depth (P=0.04) of ulcers predicted greater risk of yielding resistant bacteria. The identification of antimicrobial-resistant bacteria prompts practitioners to be prudent when choosing ophthalmic antibiotics for severe corneal ulcers.

著者

Marta DEC Anna NOWACZEK Renata URBAN-CHMIEL Dagmara STĘPIEŃ-PYŚNIAK Andrzej WERNICKI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0092, (Released:2018-06-06)

被引用文献数

22

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Campylobacteriosis is currently the most frequent zoonosis in humans and the main source of infection is contaminated poultry meat. As chickens are a natural host for Campylobacter species, one strategy to prevent infection in humans is to eliminate these bacteria on poultry farms. A study was conducted to evaluate the probiotic potential of 46 Lactobacillus isolates from chickens faeces or cloacae. All lactobacilli were able to produce active compounds on solid media with antagonistic properties against C. jejuni and C. coli, with L. salivarius and L. reuteri exhibiting particularly strong antagonism. The cell-free culture supernatants had a much weaker inhibitory effect on the growth of Campylobacter, and the neutralization of organic acids caused them to completely lose their inhibitory properties. The ability to produce H2O2 was exhibited by 93% of isolates; most of isolates had a hydrophobic surface, showed excellent survival at pH 2.0 or 1.5, and displayed tolerance to bile; 50% isolates displayed the ability to biofilm formation. Determination of MICs of various antibiotics showed that as much as 80.4% of Lactobacillus isolates were resistant to at least one antimicrobial agent. Seven ultimately selected isolates that met all the basic criteria for probiotics may have potential application in reducing Camylobacter spp. in chickens and thus prevent infections in both birds and humans.

著者

中村 義男 辻 尚利 平 詔亨

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.56, no.5, pp.1005-1007, 1994-10-15 (Released:2008-02-15)

参考文献数

9

被引用文献数

10 13

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乳頭糞線虫感染幼虫(105/kg)を暴露したウサギはパテント感染成立後に食欲が激減し, 5羽中4羽が暴露後19-33日に死亡した. 衰弱進行期においでも異常心電図は認められなかった. 乳頭糞線虫の濃厚感染はウサギに食欲減退に基づく衰弱を起こすが, 心機能には直接影響を与えないことが示唆された.

著者

Ryoko YAMADA Le Hong Thuy TIEN Sakura ARAI Mari TOHYA Kasumi ISHIDA-KUROKI Ryohei NOMOTO Hyunjung KIM Eriko SUZUKI Ro OSAWA Takayasu WATANABE Tsutomu SEKIZAKI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0083, (Released:2018-06-06)

被引用文献数

10

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Streptococcus parasuis has recently been removed taxonomically from Streptococcus suis, a zoonotic pathogen. S. parasuis has been detected in healthy pigs and in diseased pigs, which suggests that S. parasuis is involved in the normal microbiota of pigs and has potential pathogenicity. However, the pathogenicity of S. parasuis in pigs is unclear because of the lack of appropriate detection methods that discriminate S. parasuis from S. suis. In this study, we developed a PCR method that is specific for S. parasuis. The detection limit of the PCR was 350 CFU per reaction. Bacteria isolated from the saliva of eight pigs were collected and examined by PCR. Sixty-four isolates positive for PCR were obtained from the samples of all pigs. Thirteen of the 64 isolates were genetically confirmed as S. parasuis, and biologically and biochemically had nearly the same features of known S. parasuis strains, which suggested that strains positive for PCR were S. parasuis. Among the 64 isolates, 28 isolates were serotypes 20, 22, or 26 in the S. suis serotyping scheme. The remaining 36 isolates were untypeable, which suggested the presence of novel serotypes or a capsule-negative form. Therefore, the PCR method described in this study is a useful tool for identifying S. parasuis, and can be used in etiological studies on this bacterium.

著者

村田 浩一 増田 隆一

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.58, no.12, pp.1157-1159, 1996-12-25

被引用文献数

3

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外生殖器の形態からは性を判別し難いフタユビナマケモノ(Choloepus didactylus)のY染色体上性決定遺伝子(SRY)を合成酵素連鎖反応(PCR)増幅し, 仔の性鑑別をおこなった. 5ヶ月齢の仔および対照とした両親から毛を採取しDNAを抽出した. 仔および父親からSRY断片(216塩基対)がPCR増幅されたが, 母親からは増幅されなかった. ナマケモノのPCR増幅産物(166塩基対)の塩基配列を決定し, すでに報告されている他の哺乳類のSRY遺伝子配列と比較した. ナマケモノのPCR増幅産物にはそれらの遺伝子と高い相同性がみられ(74.1-86.8%), アミノ酸レベルでも同様であった(63.6-85.5%). このことから, ナマケモノのPCR増幅産物はSRY遺伝子の一部であることが推察され, 哺乳類の間で高い保存性をもっていることが分かった. この結果から仔の性別は雄と判定された. ナマケモノの毛を用いたPCR法による性鑑別は動物園での繁殖計画に役立つものである. 知る限りにおいて,貧歯目のSRY遺伝子配列に関する報告は本報が初である.

著者

Seijirow GOYA Nobuyuki KANNO Kenji TESHIMA Takanori ANNDO Takahiro FUJIOKA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0509, (Released:2018-06-06)

被引用文献数

5

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A 4-year-old, 5.9-kg female Japanese Spitz presented with syncope and exercise intolerance. Echocardiography revealed an ostium primum atrial septal defect (ASD), a cleft mitral valve, mitral valve regurgitation (MR), and tricuspid regurgitation (TR) (velocity: 3.6 m/sec, pressure gradient: 52 mmHg), leading to a diagnosis of partial atrioventricular septal defect (AVSD) with moderate pulmonary hypertension (PH). Open-heart surgery using cardiopulmonary bypass was performed through right atriotomy. The cleft of the mitral valve was sutured with polypropylene and the AVSD was closed using an autologous pericardial patch fixed with glutaraldehyde. No postoperative pulmonary hypertensive crisis occurred. Shunting flow through the ASD, TR, and PH had completely disappeared 2 months postoperatively; however, moderate MR persisted. The dog is still alive 5 years postoperatively without clinical signs.

著者

小林 創太 筒井 俊之 山本 健久 西口 明子

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.69, no.12, pp.1255-1258, 2007-12-25

参考文献数

20

被引用文献数

11

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我が国のヨーネ病摘発農場に対して全国的な疫学調査を実施し,感染農場の初摘発時におけるヨーネ病の農場内浸潤度を示唆する疫学的指標を検討した。本研究では初摘発から1年以内に感染牛が再摘発される農場では,その農場が初摘発される以前に農場内伝播が成立していると仮定し,各農場の監視期間中の追加摘発状況を観察したところ,新規に摘発された594農場のうち,158農場(27%)で再摘発が認められた.また,初摘発時の疫学情報をロジスティック回帰分析に供したところ,3つの疫学的な指標が再摘発と関連していた.すなわち,「初摘発時に発症牛がいる」,「初摘発時に複数頭摘発される」,および「牛舎形態がつなぎ飼いでない」農場は,「初摘発牛に発症牛がいない」,「初摘発頭数は1頭のみ」,および「つなぎ飼い」の農場に比べ,それぞれ3.8(95% confidence interval: 2.2,6.8),2.8(95% CI: 1.8,4.5),および2.0(95% CI: 1.1,3.6)倍再摘発されやすいことが示唆された.家畜衛生関係者にとってこれらの疫学的指標は,より集中的な防疫対策を実施するべきか否かをヨーネ病の初摘発時に判断する際の重要な指標になると思われた.

著者

Takafumi OSUMI Ikki MITSUI Akihiko MORITA Keita IYORI Koji NISHIFUJI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0128, (Released:2018-05-23)

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Localized scleroderma (LS) is a sclerotic skin disorder rarely reported in the veterinary literature. We herein report the first case of a linear LS-like skin lesion in a cat. A 1-year-old castrated male Himalayan cat was presented with a 1-month history of an alopecic, indurated, serpiginous, branched skin lesion on the dorsal cervical to scapular area. The cat had no history of trauma, although a topical spot-on endectocide had been applied near the lesion. Histopathological examination revealed a focal area of hyperplastic dermal collagen with the absence of pilosebaceous units. The cutaneous lesion remained unchanged during a 2-year follow-up period. Clinical and histopathological similarities of this skin lesion with those of the linear form of LS in humans were considered.

著者

Nguyen V. SON James K. CHAMBERS Takanori SHIGA Takuya E. KISHIMOTO Shotaro KIKUHARA Kohei SAEKI Reina FUJIWARA Masaya TSUBOI Ryohei NISHIMURA Kazuyuki UCHIDA Hiroyuki NAKAYAMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0186, (Released:2018-05-23)

被引用文献数

8

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A 12-year-old intact male Welsh Corgi was presented with enlargement of the right scrotum. Both testicles were surgically removed and histopathologically examined. On gross examination, white nodules were found in the epididymis and ductus deferens. Histopathologically, the nodules developed continuously from the tunica vaginalis testis of the right scrotum and consisted of spindle-shaped neoplastic cells that invaded the surrounding tissue. Immunohistochemically, the neoplastic cells were diffusely positive for vimentin, cytokeratin and Wilms tumor-1 (WT-1). Based on these findings, the tumor was diagnosed as sarcomatoid mesothelioma. The dog presented with respiratory distress 122 days after surgery and clinical examination found multiple metastatic lesions in the lung, abdominal lymph nodes and peritoneum. The dog died 144 days after surgery due to disease progression.

著者

伊藤 美加 長井 誠 早川 裕二 小前 博文 村上 成人 四ッ谷 正一 浅倉 真吾 迫田 義博 喜田 宏

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.70, no.9, pp.899-906, 2008-09-25

参考文献数

39

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2007年8月,石川県金沢競馬場において馬インフルエンザが発生した.感染馬の症状は主に発熱(38.2-41.0℃)および鼻汁漏出で,呼吸器症状を示した馬はわずかであった.全ての競走馬は不活化ワクチンを接種しており,ワクチンにはH3N8馬インフルエンザウイルス,アメリカ系統のA/equine/La Plata/93株,同じくヨーロッパ系統のA/equine/Avesta/93株,およびH7N7馬インフルエンザのA/equine/Newmarket/1/77株が含まれていた.鼻腔スワブ材料からH3N8馬インフルエンザが分離され,A/equine/Kanazawa/1/2007と命名した.系統樹解析では,A/equine/Kanazawa/1/2007株はアメリカ系統のフロリダ亜系統に属した.さらに,HA1サブユニットのアミノ酸解析を行ったところ,同じアメリカ系統のワクチン株であるA/equine/La Plata/93株と比較して,BおよびEの抗原決定部位に4アミノ酸の置換が認められた.また,回復期の馬16頭から採材した血清を用いて赤血球凝集抑制反応を実施したところ,A/equine/Kanazawa/1/2007株とワクチン株間に1〜3管の差が認められた.これらの結果から,流行株とワクチン株に抗原性状の差が示唆されたが,わが国の現行市販ワクチンは罹患率の低下や発症期間の短縮に貢献したと思われた.

著者

Yusuke MURAHATA Yoshiaki HIKASA Sho HAYASHI Koki SHIGEMATSU Natsuki AKASHI Tomohiro OSAKI Takeshi TSUKA Yoshiharu OKAMOTO Tomohiro IMAGAWA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0122, (Released:2018-05-21)

被引用文献数

7

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Remifentanil is an ultra-short-acting μ-opioid receptor agonist. The purpose of this study was to determine the relationship of the minimum alveolar concentration (MAC) of sevoflurane and other MAC derivatives, including the MAC for blocking adrenergic responses (MAC-BAR) and the MAC at which tracheal extubation is occurred (MAC-extubation), with or without remifentanil infusion. Six healthy adult beagle dogs were randomly anesthetized three times for determining the MAC-BAR (SEVMAC-BAR), MAC (SEVMAC), and MAC- extubation (SEVMAC-extubation) of sevoflurane under infusion of saline and remifentanil at rates of 0.15, 0.30, 0.60, 1.20 and 2.40 μg/kg/min. The ratio of the SEVMAC-BAR and SEVMAC and that of the SEVMAC-extubation and SEVMAC were not significantly different at baseline and during treatment. The MAC-BAR95 and MAC95 decreased in a dose-dependent manner until reaching 1.20 μg/kg/min, and the MAC-extubation5 decreased in a dose-dependent manner until reaching 0.60 μg/kg/min. The percentage reduction of SEVMAC-BAR, SEVMAC, and SEVMAC-extubation increased in a dose-dependent manner during remifentanil infusion. The heart rate significantly decreased in the MAC-BAR and MAC groups, and the systolic and mean arterial pressures increased after remifentanil infusion compared with the baseline values. Remifentanil infusion caused reduction of the SEVMAC-BAR, SEVMAC and SEVMAC-extubation in a dose-dependent manner, and ceiling effects were observed in the dogs. Higher doses of remifentanil and sevoflurane were necessary for blocking the sympathetic response to the supramaximal stimulus to prevent movement and extubation in dogs.

著者

Kuniko YOSHIMURA Aya MATSUU Kai SASAKI Yasuyuki MOMOI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0499, (Released:2018-05-11)

被引用文献数

2

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Sirtuin-1 (SIRT1) is a nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase with a large number of protein substrates. It has attracted a lot of attention in association with extending lifespan. The objective of this study was to enable the evaluation of SIRT1 expression in peripheral blood mononuclear cells (PBMCs) from dogs by flow cytometry. Three transcript variants were amplified from PBMCs by reverse transcription PCR and the nucleotide sequences were analyzed. On the basis deduced amino acid sequence, a monoclonal antibody against human SIRT1, 1F3, was selected to detect canine SIRT1. Canine SIRT1 in peripheral blood mononuclear cells was successfully detected by western blotting using this antibody. Intracellular canine SIRT1 was also detected in permeabilized 293T cells transfected with a canine SIRT1 expression plasmid by flow cytometry using this antibody. SIRT1 was detected in all leukocyte subsets including lymphocytes, granulocytes and monocytes. The expression level was markedly different among individual dogs. These results indicated that the method applied in this study is useful for evaluating canine SIRT1 levels in PBMCs from dogs.

著者

SeongJin YUN Seonmi KANG Kangmoon SEO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0114, (Released:2018-05-04)

被引用文献数

1

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A 5-year-old castrated male poodle presented with blindness. Ophthalmic examinations including slit-lamp biomicroscopy, tonometry, ultrasonography, and electroretinography, were performed. The anterior lens capsule of the right eye (OD) was totally pigmented, with persistent pupillary membranes (PPMs). Ultrasonography of the same eye showed severe lens atrophy and retinal detachment. Electroretinography revealed flat a- and b-waves in OD, but normal amplitudes in the left eye (OS). No ocular defects were detected in OS except mature cataract. In this case, it was determined that hypermature cataract with PPMs, caused both lens-induced-uveitis and total anterior lens capsule pigmentation. This condition needs to be differentiated from absent pupil. Notably, PPMs with total anterior lens capsular pigmentation are extremely rare in dogs.

著者

Doaa SALMAN Wilawan PUMIDONMING Eiji OOHASHI Makoto IGARASHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0713, (Released:2018-05-02)

被引用文献数

18

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The present study investigated the prevalence of Toxoplasma gondii and other intestinal parasites in cats in the Tokachi subprefecture in Japan. A total of 365 household cats were included in the study, and 353 serum and 351 fecal samples were collected and analyzed. T. gondii IgG antibodies were detected in the sera of 16.14% of cats based on Latex agglutination test and ELISA. For ELISA, T. gondii RH strain tachyzoites lysate and T. gondii SAG2 recombinant protein were used as antigens. Low seropositivity was detected in cats younger than one year and older than 11 years; outdoor and hunter cats showed significantly high seropositivities. Neutering either in male or female cats, but not gender, had a considerable effect on seroprevalence. Toxoplasma gondii oocysts were detected in one fecal sample. The overall parasitic infestation in cats was 12.5%. Other detected parasites included Toxocara species, which showed the highest prevalence of 7.7%, followed by Isospora spp. (2%), Taenia spp. (1.7%), and Ancylostoma spp. (0.9%). Spirometra spp. was detected in only one sample. Outdoor cats comprised 50% of all 44 parasite-infested cats. Although T. gondii oocysts were detected in only one sample, the relatively high seroprevalence of T. gondii indicated that it can pose significant risks to the environment. Our findings highlighted the potential of outdoor cats as a source of T. gondii and other parasites.

著者

芒 来 和田 隆一 九郎丸 正道 杉浦 健夫 吉原 豊彦 及川 正明 林 良博

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.62, no.9, pp.1001-1003, S・iii, 2000-09-25

参考文献数

13

被引用文献数

7

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馬喉嚢の機能を解明する糸口を得るため, 粘膜における各種免疫グロブリンのアイソタイプおよびサブアイソタイプの分布を調べた.その結果, IgGaおよびIgM含有細胞は粘膜のリンパ小節と粘膜下リンパ小節に認められ, IgGcは粘膜下リンパ小節にのみ検出された.これらはいずれもリンパ球や形質細胞内に認められた.一方, IgAは前記遊走細胞に加えて腺上皮や粘膜上皮表層に検出されたことから, 腺上皮から分泌される分泌型IgAの存在が示唆された.

著者

Panpicha SATTASATHUCHANA Prangtip PHUWAPATTANACHART Naris THENGCHAISRI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0443, (Released:2018-04-24)

被引用文献数

5

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The objective of this study was to evaluate the efficacy of a non-selective COX inhibitor (tolfenamic acid) and a selective COX-2 inhibitor (robenacoxib) for post-operative pain control in cats. Thirty cats undergoing ovariohysterectomy were randomly divided into three groups: the control (placebo) group, the tolfenamic acid (4 mg/kg/day) group, and the robenacoxib (1 mg/kg/day) group. Non-steroidal anti-inflammatory drugs (NSAIDs) were administered orally 2 hr before anesthesia induction and 24 and 48 hr post-operation. Buccal mucosal bleeding times (BMBTs) were assessed prior to anesthesia induction. Colorado pain scores and composite pain scores were evaluated in a blinded fashion before induction and 2, 8, 24, 30 and 48 hr post-operation. The Colorado pain scores of cats receiving robenacoxib were significantly lower than those of cats in the control group at 30 (P=0.0126) and 48 (P=0.0439) hr post-operation. The composite pain scores of cats from the robenacoxib group were lower than those of cats in the control group at 30 (P=0.0299) and 48 (P=0.01030) hr post-operation. The Colorado pain scores of cats receiving tolfenamic acid were significantly lower than those of cats in the control group at 30 hr (P=0.0186) post-operation. The composite pain scores in cats in the tolfenamic acid group were lower than the scores of cats in the control group at 24 (P=0.0403) and 48 (P=0.0413) hr post-operation. BMBTs remained within normal limits in all groups. Both tolfenamic acid and robenacoxib are useful for post-operative pain control in cats.

著者

Chunqiu LI Donghua GUO Rui WU Fanzhi KONG Junjun ZHAI Dongwei YUAN Dongbo SUN

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0559, (Released:2018-04-24)

被引用文献数

7

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To trace the prevalence of canine distemper virus (CDV) in diarrhoetic dogs, a total of 201 stool samples were collected in the Heilongjiang province of northeastern China from May 2014 to April 2015. The 201 fecal samples were subjected to the detection of CDV by using RT-PCR targeting the partial N gene, phylogenetic analysis based on the complete H gene, and co-infection analysis. Results indicated that 24.88% (50/201) of the samples were positive for CDV. The fifty CDV samples exhibited an overall co-infection rate of 94% (47/50) with four enteric viruses (82%, 41/50) and five bacteria (72%, 36/50). The positivity rate of CDV exhibited differences among regions, seasons, ages, and immunization status. Phylogenetic analysis of the complete H genes (n=6) revealed that the CDV strains identified in our study belonged to the Asia-1 group, and showed genetic diversities. These data provide evidence that there are a number of genetically diverse CDV Asia-1 strains circulating in diarrhoetic dogs in northeastern China; the CDV-affected animals exhibit the high co-infection with other enteric viruses and bacteria.

著者

Tomoya MORITA Kensuke NAKAMURA Osuga TATSUYUKI Atsushi KOBAYASHI Osamu ICHII Akira YABUKI Mitsuyoshi TAKIGUCHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0716, (Released:2018-04-23)

被引用文献数

3

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A 12 year-old intact male Pembroke Welsh corgi weighing 10.8 kg was presented for evaluation of a 3-month history of dyspnea, and a 1-week history of exercise intolerance and anorexia. Severe hypoxemia (PaO2 56 mmHg), diffuse lung alveolar infiltration, and severe pulmonary hypertension (tricuspid regurgitation pressure gradient was 81 mmHg) were identified. A tentative diagnosis of severe PH due to lung disease or pulmonary thromboembolism was made and treated intensively. After 5 days of hospitalization, the dog died despite oxygen supplementation and anticoagulant therapy. This dog was diagnosed as unclassified interstitial lung disease based on histopathological findings.

著者

Jin-A LEE Yun-Mi KIM Young-Kyu PARK Young-Cheol YANG Bock-Gie JUNG Bong-Joo LEE

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0236, (Released:2018-04-16)

被引用文献数

50

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Black soldier fly (Hermetia illucens) larvae (BSFL) are rich in protein and have the potential to be used in animal feed. The aim of the present study was to determine the immunoprophylactic effect of BSFL against Salmonella Gallinarum in broiler chicks as an alternative feed additive. Results showed that BSFL improved body weight gain and increased frequency of CD4+ T lymphocyte, serum lysozyme activity, and spleen lymphocyte proliferation. Moreover, BSFL reinforced bacterial clearance and increased survivability of broiler chicks against S. Gallinarum. These data suggested that BSFL has prophylactic properties with stimulating non-specific immune responses, as well as reduced bacterial burden against S. Gallinarum.