著者

藤田 和弘 貴田 明宏 稲垣 昭生

出版者

Japanese Association of Forensic Science and Technology

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.11, no.1, pp.29-40, 2006

被引用文献数

4 2

---

JPEG images are degraded by blocking artifacts and mosquito noise caused by quantization on the block DCT domain. The purpose of this paper is reduction of image quality degradation by means of the image restoration method instead of the smoothing method after the simple decoding. This proposed method is based on the edge-adaptive restoration method; the regularizing operator depends on the edge orientation, and the regularizing parameter depends on the local activity. The variance of the quantization on the block DCT domain is taken into consideration.

著者

藤田 和弘 貴田 明宏 稲垣 昭生

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.11, no.1, pp.29-40, 2006 (Released:2007-01-19)

参考文献数

11

被引用文献数

1 2

---

JPEG images are degraded by blocking artifacts and mosquito noise caused by quantization on the block DCT domain. The purpose of this paper is reduction of image quality degradation by means of the image restoration method instead of the smoothing method after the simple decoding. This proposed method is based on the edge-adaptive restoration method; the regularizing operator depends on the edge orientation, and the regularizing parameter depends on the local activity. The variance of the quantization on the block DCT domain is taken into consideration.

著者

小出 健次 児玉 智章

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.24, no.1, pp.1-13, 2019 (Released:2019-01-31)

参考文献数

47

---

Motorization produced traffic accidents and its conflicts to be brought into the court of law. The field of accident reconstruction was born to cope with these litigations. Essentially, traffic accident is a crime of negligence. Punishment and insurance payment depend upon the assessment of the case. Vehicle speed plays an important role in the ratio of fault and plays an important role as a standard of fault authorization. An analysis method has been developed ever since. Now in Japan, traffic accident is punished with a special law independent from the existing penal code. Speed analysis has become a more important factor under this law. Therefore speed analysis has a long historical path and still plays an important field within forensic science. Fundamental method of speed analysis has been long based on the application of basic physics principles. Recent evolution of electrical devices brought another approach to the speed analysis. Images of CCTV, drive recorders, and vehicle mounted electrical data are offering a good chance to analyze vehicle speed. We would like to review the speed analysis of the traditional methods and overview the trend of recent methods of the new era.

著者

小野 孝明 友成 航平 森 名生 冨阪 吉登 西 英二

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

pp.686, (Released:2014-12-11)

参考文献数

18

被引用文献数

6 3

---

Accurate DNA quantification is required for the short tandem repeat typing of forensic samples. Various commercial DNA quantification kits for forensic DNA analysis have recently been released. However, DNA quantification may fail if too much PCR inhibitor is extracted with the target DNA sample. Therefore, this study experimentally evaluated the degree of the alteration of DNA quantification results of various commercial DNA quantification kits (Takara RR281, Quantifiler Duo DNA Quantification Kit, Plexor HY System, Investigator Quantiplex HYres Kit, Quantifiler Trio DNA Quantification Kit, and Quant-iT dsDNA HS Assay Kit) in the presence of various concentrations of PCR inhibitors such as hematin, humic acid, indigo carmine, melanin, and tannic acid. Compared to the other kits, the DNA quantification abilities of the Investigator Quantiplex HYres Kit and Quant-iT dsDNA HS Assay Kit were not negatively affected by high concentrations of hematin. The DNA quantification abilities of the Quantifiler Trio DNA Quantification Kit and Quant-iT dsDNA HS Assay Kit were not negatively affected by high concentrations of PCR inhibitors containing humic acid, indigo carmine, or melanin. The DNA quantification abilities of the Investigator Quantiplex HYres Kit and Quantifiler Trio DNA Quantification Kit were not negatively affected by high concentrations of tannic acid. Furthermore, the DNA quantification ability of the Quantifiler Trio DNA Quantification Kit was more reproducible than those of the other kits. Moreover, this kit was able to assess the degree of DNA degradation by comparing quantified both the short amplicon (80 bp) and long amplicon (130 bp). However, the short amplicon was not negatively affected by high concentrations of PCR inhibitors in contrast to the long amplicon. These results indicate forensic DNA analysts should select the appropriate DNA quantification kits that are unaffected by PCR inhibitors and carefully interpret DNA quantification results.

著者

吉田 浩陽 鈴木 真一

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.16, no.1, pp.49-55, 2011 (Released:2011-03-05)

参考文献数

8

---

In Japan, regular gasoline and premium gasoline are used as fuel for motor vehicles. Sometimes an accident will occur in which regular gasoline was filled by mistake instead of premium gasoline and discrimination between regular and premium gasoline is required in the investigation process. According to the standards, regular and premium gasolines are different only in octane value. Since measuring octane value requires a special device, an alternative method for determining a gasoline sample as a regular or premium one is desired. In this paper a new method for discriminating between regular and premium gasoline utilizing gas chromatography/mass spectrometry (GC/MS) is proposed. Analyzation of regular and premium gasoline collected in December 2007, March, June and September 2008 from gas stations of 15 different brands in northwestern Chiba and eastern Saitama prefecture revealed that, compared to regular gasoline, premium gasoline was rich in alkylate while smaller in 2- and 3-methylhexane concentration. All 120 gasoline samples were correctly classified as either regular or premium gasoline by measuring the ratio of alkylate to methylhexane fractions.

著者

大塚 祐輔 平間 一樹 横田 賀英子 渡邉 和美 和智 妙子

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.22, no.1, pp.25-34, 2017 (Released:2017-01-28)

参考文献数

20

被引用文献数

1

---

The present study compared decision tree analysis to logistic regression analysis in order to investigate whether decision tree analysis has sufficient ability to construct a model that predicts offender characteristics from the crime scene and/or victim information. The data used in this study were collected from solved single homicide cases that occurred in Japan between 2004 and 2009 (n=1226). After constructing models that predict offender's criminal history by logistic regression analysis and decision tree analysis, AUC (area under the ROC curve) of those models and the predictive values were compared. The AUC was .75 (p<.001) for logistic regression model and .71 (p<.001) for the decision tree model. A significant difference between these AUCs was not observed (χ2(1)=3.71, p=.05). The predictive values were 67.3% for both the logistic regression model and the decision tree model. These findings suggest that the decision tree is comparable to logistic regression analysis in constructing a model that predicts the offender's criminal history from offence characteristics in single homicide cases.

著者

小野 孝明 友成 航平 森 名生 冨阪 吉登 西 英二

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.20, no.1, pp.69-81, 2015 (Released:2015-02-10)

参考文献数

18

被引用文献数

3

---

Accurate DNA quantification is required for the short tandem repeat typing of forensic samples. Various commercial DNA quantification kits for forensic DNA analysis have recently been released. However, DNA quantification may fail if too much PCR inhibitor is extracted with the target DNA sample. Therefore, this study experimentally evaluated the degree of the alteration of DNA quantification results of various commercial DNA quantification kits (Takara RR281, Quantifiler Duo DNA Quantification Kit, Plexor HY System, Investigator Quantiplex HYres Kit, Quantifiler Trio DNA Quantification Kit, and Quant-iT dsDNA HS Assay Kit) in the presence of various concentrations of PCR inhibitors such as hematin, humic acid, indigo carmine, melanin, and tannic acid. Compared to the other kits, the DNA quantification abilities of the Investigator Quantiplex HYres Kit and Quant-iT dsDNA HS Assay Kit were not negatively affected by high concentrations of hematin. The DNA quantification abilities of the Quantifiler Trio DNA Quantification Kit and Quant-iT dsDNA HS Assay Kit were not negatively affected by high concentrations of PCR inhibitors containing humic acid, indigo carmine, or melanin. The DNA quantification abilities of the Investigator Quantiplex HYres Kit and Quantifiler Trio DNA Quantification Kit were not negatively affected by high concentrations of tannic acid. Furthermore, the DNA quantification ability of the Quantifiler Trio DNA Quantification Kit was more reproducible than those of the other kits. Moreover, this kit was able to assess the degree of DNA degradation by comparing quantified both the short amplicon (80 bp) and long amplicon (130 bp). However, the short amplicon was not negatively affected by high concentrations of PCR inhibitors in contrast to the long amplicon. These results indicate forensic DNA analysts should select the appropriate DNA quantification kits that are unaffected by PCR inhibitors and carefully interpret DNA quantification results.

著者

桐原 美穂 川端 三十一

出版者

Japanese Association of Forensic Science and Technology

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.15, no.1, pp.59-64, 2010

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Cannabis seeds not pretreated for prevention of germination are on sale through the Internet in recent years. These seeds are illegal and show a high probability of germination. The number of arrestees, who had cultivated illegal Cannabis seeds, has increased year after year.<br>   Recently, we had an opportunity to examine Cannabis seeds. We carried out experiments for the proof of the germination abilities by usual and embryo-exposure techniques. The embryo-exposure technique is a speedy and effective method for germination of Cannabis seeds, especially for the weakened seeds.<br>   In this study, we have reported and recorded the course of germination, how to determine the germination and the growth process of Cannabis.<br>

著者

室 友紀 今村 真二 中村 博明 長谷川 正紀 湯浅 勲

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.15, no.2, pp.143-149, 2010

被引用文献数

1

---

&nbsp;&nbsp;In many countries, including Japan, cultivation of <i>Cannabis sativa</i> (<i>C. sativa</i>) for drug use has been illegal and prohibited. Recently, seeds for cultivation purposes are easily available from internet shops, and then we have often been requested to identify <i>C. sativa</i>. The identification has conventionally been performed by morphological and chemical tests. But, it can be difficult to identify tiny and fragmenting samples as <i>C. sativa</i> even if these tests are performed.<br> &nbsp;&nbsp;In this study, we aimed to establish a method based on DNA analysis. As an initial step, we attempted a method reported by Linacre et al, however, cross-amplification between <i>C. sativa</i> and <i>Humulus lupulus</i> (<i>H. lupulus</i>) with <i>C. sativa</i> specific primers (G and H) was observed. To avoid this cross-amplification, we designed a new primer specific for <i>C</i>. sativa (cp-Can) on <i>trnL</i> intron of chloroplast DNA. DNA samples from nine plants including <i>C. sativa</i> and <i>H. lupulus</i> were amplified using the green plant universal primer pair and the cp-Can. After subsequent agarose gel electrophoresis, <i>C. sativa</i> DNA showed two bands, whereas the other plant DNA showed one band, indicating the clear distinction from the other plants tested. In addition, a BLAST search with the cp-Can sequences showed no cross-activity with other plants. The present method is very simple, rapid, sensitive, and useful for the identification of <i>C. sativa</i>.<br>

著者

財津 亘

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.15, no.2, pp.111-124, 2010 (Released:2010-08-27)

参考文献数

23

被引用文献数

1 1

---

Serial arsonists (N=125) were differentiated into four groups on the basis of their social independence and the degree of criminality, by using categorical principal components analysis (CATPCA). Results indicated the following. (1) Arsonists with high social independence and high criminality were aged 40 years or more in age, did not live with their parents, and tended to have criminal records for theft. (2) Arsonists with high social independence and low criminality were most frequently employed, high school graduates, married, and had no criminal record. Moreover, there were more female and mental patients in this group compared with other groups. (3) Arsonists with low social independence and high criminality were all male, compulsory education level (including high school dropouts), unmarried, not living with parent(s), and had no criminal record of theft. These arsonists tended to have relatively no criminal record of arson compared with other groups. (4) Arsonists with low social independence and low criminality were the youngest among four groups and were aged between 10 and 30 years, unmarried, lived with their parent(s), and had no criminal record. Results of log-linear analysis indicated that arsonists with high social independence tended to use a car or walk to the crime scene, whereas those with low social independence were inclined to use a bicycle. Moreover, arsonists with high criminality records tended to prepare the medium for arson in advance and drink alcohol before the offence, whereas arsonists with low criminality had a tendency to set fire to the same place repeatedly.

著者

横田 賀英子 大塚 祐輔 倉石 宏樹 和智 妙子 渡邉 和美

出版者

Japanese Association of Forensic Science and Technology

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.19, no.1, pp.19-30, 2014

---

Characteristics of male offenders arrested in Japan for exhibitionism (<i>N</i> = 414) and features of their offences were investigated. In analyses, their behavioral consistency was also examined by focusing on 87 repeat exhibitionists. The results indicated that many offenders were well-adjusted persons: 71% were employed, 30% were married, and 23% were graduates. Moreover, 66% of the offences were committed in daytime, and 64% targeted victims aged 10-20 years. In 24% of cases, the distances between offenders' residences and crime scenes were less than one kilometer, whereas 40% of offenders lived over 5 kilometers away. In terms of behavioral consistency, offences in "trains or buses" and public spaces were shown to be consistent for repeated arrests, even after taking the occurrence rate into consideration by measures of adjusted standardized residual (ASR) and forward specialization coefficient (FSC), which is suggestive of behavioral consistency. Finally, less frequent behaviors, such as nighttime offences and offences targeting victims aged 30 years or older occurred less consistently in the series of offences. The results suggested that many exhibitionists rationally decided when and where to commit crimes, based on their calculation of risk and reward. The fact that many offenders were well-adjusted persons might be one of factors explaining offender's rational decision making. The findings in the current study can contribute to the police investigation to prioritize their investigative targets. The current research can form the basis of understanding of the nature of male exhibitionists.<br>

著者

肥田 宗政 伊藤 健次郎 三井 利幸

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.11, no.2, pp.185-191, 2006 (Released:2007-01-19)

参考文献数

3

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A mixed solution of 28% of ammonia and pyridine (9 : 1 (v/v)) was used to extract dye ink from counterfeit paper money printed by an ink-jet printer. The counterfeit paper money was fixed on a vacuum filtration device instead of a membrane filter. The extracted solution was collected in a test tube and was evaporated to dryness under reduced pressure in a 40°C water bath. The extract was dissolved by 10 μl of the extraction solution and spotted on a silica gel plate. The silica gel plate was developed by iso-propanol : 28% ammonia : pyridine (2 : 9 : 1 v/v/v). By comparison with TLC patterns, we could clearly distinguish among the various printer companies. Furthermore the TLC pattern was discriminated between the machine types to some extent.

著者

大城 圭太 齊藤 卓也 大和田 幸延 内藤 春顕 松島 裕 磯崎 翔太郎 垣本 由布 大澤 資樹

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.28, no.2, pp.183-187, 2023 (Released:2023-07-31)

参考文献数

9

---

This article aims to evaluate estimation of the postmortem submersion interval (PMSI) for cadavers recovered from the Pacific Ocean in the South Kanto area of Japan. Data were collected from crime scene reports in the 3rd Regional Coast Guard Headquarters over a 11-year period. Approximate time of PMSI was available for 72 cases with the decomposition stage from photo pictures at the scene. No visible changes in the skin of the hands and feet counted from 27 cases, wrinkling and discoloration in 28 cases, and degloving in 17 cases. The average (±S.D.) of accumulated degree days (ADD) was 2.0±3.3, 24.5±19.1, 164.0±112.5, respectively. As minimum ADD, wrinkling and degloving were expected to be formed in 9.4 and 77.8, respectively, which corresponded to a half day and four days at 20°C of sea water temperature. In comparison with other findings, degloving was accompanied by bloated abdomen, sloughing of hairs, and peeling of nails. The characteristic changes of the hands and feet were helpful to estimate PMSI for submerged cadavers. We expect to apply this finding to criminal investigation at the scene.

著者

石丸(飯尾) 麗子 岩室 嘉晃 地中 啓 高山 成明 早川 和一

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.18, no.2, pp.155-166, 2013 (Released:2013-07-30)

参考文献数

25

被引用文献数

1 1

---

Chiral analyses of methamphetamine (MA) and its metabolites of urine samples from 250 d-MA abusers were done by capillary electrophoresis/mass spectrometry (CE/MS) with direct injection of urine. We examined the relationships between the concentrations of unchanged MA and metabolites including the conjugates and the intake situations of MA (e.g. date, route) based on statements of abusers.

著者

桑山 健次 辻川 健治 宮口 一 金森 達之 岩田 祐子 井上 博之 岸 徹 角田 紀子

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.10, no.2, pp.127-133, 2005 (Released:2007-07-03)

参考文献数

12

被引用文献数

1 1

---

The effects of the various preparation procedures of the Dragendorff reagent on sensitivity for thin layer chromatography (TLC) were examined. The sensitivity to various compounds was different depending on the preparation procedures of the Dragendorff reagent.   The reagent with the highest sensitivity to most of the compounds tested was the one prepared with bismuth subnitrate and concentrated hydrochloric acid. However, color spots were disappeared relatively in a short time after the reagent was sprayed on the compounds. The reagent with the second highest sensitivity was the one prepared with precipitation of Bi(OH)3 from bismuth subnitrate, although the procedure was complicated and time-consuming.   Consequently, to simplify the preparation procedure of the reagent, we modified it to the procedure without precipitation of Bi(OH)3 from bismuth subnitrate. The reagent was also prepared from commercially-manufactured Bi(OH)3 or BiI3. The modified Dragendorff reagents showed almost the same sensitivity to most of the compounds tested as the one prepared with precipitation of Bi(OH)3 from bismuth subnitrate, and would be useful for practical TLC analysis.

著者

岡田 侑己 瀬川 尋貴 山室 匡史 桑山 健次 辻川 健治 金森 達之 岩田 祐子

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.28, no.1, pp.51-58, 2023 (Released:2023-01-31)

参考文献数

9

被引用文献数

1

---

We examined the decomposition behavior of 1-acyl-substituted derivatives of d-lysergic acid diethylamide (LSD), such as 1-cyclopropanecarbonyl LSD (1cP-LSD), 1-acetyl LSD (ALD-52), and 1-propionyl LSD (1P-LSD) through gas chromatography/mass spectrometry (GC/MS). It was reported previously that in methanol, 1cP-LSD completely decomposed into LSD during GC/MS. We found that in methanol, 1cP-LSD remained undecomposed during GC/MS, and the extent of decomposition varied based on the analyses performed in this study. As a result of a detailed examination, we deduced that the decomposition occurs at the inlet, regardless of the inlet temperature or the type of the inlet liner. We observed that the peak areas of 1cP-LSD decreased with the deterioration of the inlet liner, and this was considered to be a cause for the variation between different analyses. While the acetonitrile solution of 1cP-LSD provided relatively robust results, the other examined solvents showed a significant decomposition of 1cP-LSD and/or a sequent decrease in the peak area of 1cP-LSD with time after the replacement of the inlet liner. ALD-52 or 1P-LSD in acetonitrile were stable during GC/MS, however, they were unstable when methanol was used as a solvent, similar to 1cP-LSD. This suggested that a similar decomposition and/or a sequent decrease in the peaks of ALD-52 and 1P-LSD during GC/MS can be expected.

著者

恒吉 宏亮 平田 京一郎 鈴木 健一郎 髙橋 志宗 飛田 祐志 上田 篤 渡邊 慎太郎 市川 俊和 仲川 政宏 萩原 隆男 本間 正勝 岡本 勝弘

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.27, no.2, pp.137-150, 2022 (Released:2022-07-31)

参考文献数

15

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In the case of a fire incident such as arson, the combustion experiment that reconstructed the fire scene will be conducted to clarify the facts. However, various problems such as construction costs and securing an experimental environment must be dealt with. Therefore, the purpose of this study is to utilize fire simulation for the investigation of fire incidents. In this study, combustion experiments and FDS (Fire Dynamics Simulator) simulations assuming gasoline arson in the kitchen, which have not been reported so far, were conducted and compared. Next, the observation of fire damage using the accumulated heat-flux was evaluated by a newly developed analysis tool for visual evaluation. The simulation results such as burning behavior, temperature changes, and the burned away phenomenon of the door showed good agreement with those experimental results. Burning behavior in the kitchen, that couldn't be observed in the combustion experiment were grasped in detail in the simulation. Furthermore, the relationship between the fire source and the fire damage on the floor, which couldn't be explained only by the observation results after extinguishing the fire, could be reasonably explained by the visual evaluation of burning behavior and the accumulated heat-flux in the kitchen by using the simulation. Therefore, the fire simulation was shown to be an effective tool for analyzing fire phenomena.

著者

田中 俊輔 大野 友則

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

pp.685, (Released:2015-06-15)

参考文献数

15

被引用文献数

1

---

When traffic accidents happen, investigators observe the state of the vehicle at the crash site of the accident. Here the energy conservation law can be used to estimate the vehicle collision speed. Since the collision speed of the vehicle can be estimated from the energy of its deformed volume, investigators observe and measure the vehicle's crash state. However, when a vehicle collides into traffic signs, both the vehicle and sign will be deformed. Consequently, to estimate correctly the vehicle collision speed, the energy absorption capacity of the signs should be taken into account with the vehicle deformation after the crash.   In this study, to improve the estimation of the collision speed of the vehicle, the drop weight tests were done to examine the absorption energy of the signs. The traffic signs used in this test are a road sign, a road mirror and a guard-rail on the roadside. From test results, it can be concluded that the input energy is linearly proportion to the deformed angle of the sign. Also from this test, the relationships between energy and deformed angle of the signs can be formulated. Identification for estimating the vehicle collision speed at an accident hereafter can be done properly with ease.

著者

山室 匡史 岡田 侑己 瀬川 尋貴 桑山 健次 辻川 健治 金森 達之 岩田 祐子

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

pp.837, (Released:2022-06-16)

参考文献数

10

---

In Japan, forensic examination of cannabis seeds requires proof of “being cannabis” and “being germinable”, and is currently conducted by continuing cultivation of the seeds for several weeks after germination tests and performing morphological observations and chemical analysis on the true leaves. We have previously constructed a rapid and simple method for identification of cannabis seeds by combining the color reaction with DNA testing. In this study, the effectiveness of the cannabis seed identification methods by combining the color reaction, germination test, and DNA testing that does not involve a cultivation process was verified on seized cannabis seeds. As previously reported, the combination of the color reaction using 2,3,5-Triphenyl-2H-tetrazolium Chloride and DNA testing using a commercial kit proved that the seized materials were germinable cannabis seeds within one day. Furthermore, the germination test, in which the young roots and cotyledons were visually checked one week after sowing, was able to more directly confirm germination ability. In addition, DNA testing was possible for the young roots after germination, indicating that they were cannabis. This study shows that the methods of cannabis seed identification, which does not involve a cultivation process, are effective even for seized materials whose storage conditions are unknown.

著者

山室 匡史 宮本 重彦 立入 直紀 石井 歩 松田 駿太朗 岩田 祐子 瀬川 尋貴 桑山 健次 辻川 健治 金森 達之 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.26, no.1, pp.29-48, 2021 (Released:2021-01-31)

参考文献数

31

被引用文献数

3 1

---

The forensic identification of cannabis is performed by a combination of chemical analysis and morphological examination. Recently, molecular biological analysis using cannabis DNA information has been noticed as a new approach. In this study, the cannabis DNA detection kit using a DNA chromatography chip was developed, and the demonstration evaluation in the forensic chemical laboratory was carried out. The DNA detection kit of a “four-line version” which had the function to distinguish fiber-type from drug-type cannabis showed as high accuracy (98.3%) as the current identification method on cannabis identification. However, there was a tendency to mistake a part of the drug-type samples as “fiber-type cannabis”. In the kit of a “three-line version” which was specialized for the cannabis DNA detection, the accuracy of 99.0% was confirmed on the cannabis identification. There were no false positives throughout all evaluations. In addition, some of the combustion residues that could not be identified as cannabis by the current identification method were classified to be “cannabis positive” by the DNA detection kit, indicating the effectiveness of a new approach. As a result of this study, it was shown that the quick and accurate cannabis DNA analysis could be carried out by the DNA detection kit even by analytical chemists who didn't have expertise in molecular biology.