著者

小前 憲久 日比野 康英 菅野 延彦

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.119, no.10, pp.763-772, 1999-10-01

被引用文献数

2

---

The spectral karyotyping (SKY) method is a novel molecular cytogenetic technique which simultaneously discerns entire chromosomes. In order to elucidate the origins of micronuclei induced under hyperthermic conditions in human lymphocyte culture, peripheral blood cells were cultured at 40℃ or 42℃ for 3-24 h, using the cytokinesis-block method with cytochalasin B. The induced micronuclei were identified by the fluorescence in situ hybridization (FISH) and SKY methods. At 42℃ for more than 6 h, the frequency of occurrence of micronuclei in binucleated cells rose with increasing incubation time. By the FISH method, 83.3% of micronuclei induced in 24 h culture at 42℃ were shown to be positive for the human centromeric probes. By the SKY method, each micronucleus induced under the hyperthermic conditions was identified unequivocally and shown to contain a specific chromosome. These results suggest that the micronuclei induced under the hyperthermic conditions in human lymphocyte culture contain chromosomes which do not migrate to the poles at the anaphase of the cell cycle because of the breakdown of the spindle apparatus.

著者

津田 恭介 伊藤 四十二

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.84, no.10, pp.905-910, 1964

著者

岡 希太郎

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.100, no.3, pp.227-240, 1980

被引用文献数

1

---

Synthetic studies on salamander alkaloids isolated from animal venom in the skin gland of Salamandra maculosa LAURENTI were performed. Syntheses of three groups of alkaloids, one with a bicyclo-oxazolidine, one with a carbinolamine, and another with a pure 3-aza-A-homo steroid, are discussed. Regio- and stereospecific syntheses of the alkaloids are discussed in connection with the construction of the bicyclo-oxazolidine system of the major alkaloids and in connection with Beckmann rearrangement of steroid 3-ketoximes for the syntheses of the other alkaloids. A theoretical approach to the resolution of the geometrical isomers of oximes by adsorption chromatography is also discussed.

著者

安茂 寿夫 和志 武三徳 漆崎 文男 野田 国男

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.88, no.11, pp.1404-1411, 1968

---

2, 2-Diphenyl-2-propargyloxyacetic acid (2-dimethylaminoethyl) ester hydrochloride (DPDM) is decomposed in aqueous solution. It was confirmed by thin-layer chromatography and gas chromatography that the decomposition of DPDM occurs as consecutive reactions. In the first step, DPDM is hydrolysed to propargyloxy-benzilic acid (BPE) and β-dimethylaminoethanol hydrochloride and next, BPE is decomposed to benzilic acid (BA) and propargyl alcohol. As BPE is extremely slightly soluble in water, BPE precipitates in aqueous solution in proportion to decomposition of DPDM. In the present investigation, the hydrolytic decomposition of DPDM and BPE was studied in order to obtain more stable aqueous preparations, and DPDM was found to be stable at pH 2.5 to 3.5. Variations in the concentration of DPDM, BPE, and BA in aqueous solution were calculated by an analog computer. Data observed in the experiment corresponded with those calculated by the computer.

著者

竹本 常松 草野 源次郎 ヒキノ ヒロシ

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.86, no.12, pp.1160-1161, 1966-12-25

---

From the herbs of Youngia denticulata (Japanese name "Yakushi-so")(Compositae), germanicyl acetate (I), taraxasteryl acetate (II), germanicol (III), taraxasterol (IV), β-sitosterol (V), and 1-hexacosanol (VI) were isolated.

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.1921, no.477, pp.App5, 1921-11-26 (Released:2018-08-30)

著者

金森 久幸 森本 一義 木苗 直秀 富田 勲

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.101, no.7, pp.596-604, 1981

被引用文献数

1 2

---

Several amino acids and ammonia were allowed to react with L-ascorbic acid or D-glucose in 0.1 M phosphate buffer (pH 7.0) at 37°C for 2 months. The reaction mixtures were submitted to mutagenic assay and structural analysis. The browning products from L-tryptophan (0.25M) and L-ascorbic acid (1M) showed a DNA-damaging potency to B. subtilis. They also gave a mutagenic activity to S. typhimurium TA 100 but not TA 98. By extracting the reaction products with benzene and ethyl acetate, followed by purifying them through silica-gel column and thin layer chromatographies (Kiesel-gel 60), 6 β-carbolyl and 4 furyl derivatives were identified. Among them, 1-(2-furyl)-9H-pyrido-[3, 4-b] indole exhibited a mutagenic activity to S. typhimurium TA 100 with or without S-9 mixture and 1-(2-furyl)-9H-pyrido [3, 4-b] indole-3-carboxylic acid showed the activity to S. typhimurium TA 100 only in the absence of S-9 mixture.

著者

安永 峻五 下村 脩

出版者

日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.74, no.7, pp.778-780, 1954

---

In electrochromatography, five kinds of different filter paper holders were used and the velocity of migration, area of colored band, and migration distance of each position on the filter paper were measured under identical conditions so as to examine the separatory ability and equality of rate of each apparatus. It was thereby found that the best separation is effected by placing the paper in a convex form, with the middle higher, while for uniform mobility, the filter paper should be cooled directly by closely adhering the lower surface on a cooling vat.

著者

安永 峻五 下村 脩

出版者

日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.74, no.1, pp.62-66, 1954

被引用文献数

2

---

A filter paper wetted with an electrolyte was placed between two glass plates coated with a silicone resin. Electric current was passed through this paper in order to find the relationship between the time, voltage, electric current, and temperature with degree of mobility of numerous inorganic ions. It was found that a linear relation always existed, in any of the electrolyte used, between the distance of mobility and time and voltage. The effect of electric current was examined with sodium nitrate as an electrolyte and it was found that electric current did not influence the mobility of the ions as much as voltage. A metallic thermostatic vat, coated with insulating paint, was devised and the mobility of ions at various temperatures was examined and it was found that the mobility generally increased with the increase of temperature and this influence was found to be greater the larger the mobility of the ion. Influence of evaporation by the warming of the filter paper was quantitatively examined, using the Durrum apparatus, with either direct or alternative current as the electric source, from the relationship between the position of the sample placed and the distance of ionic mobility. It was thereby unexpectedly found that a great deal of effect was caused by evaporation.

著者

安永 峻五 下村 脩

出版者

日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.73, no.12, pp.1350-1353, 1953

被引用文献数

3

---

By the use of the same method of capillary filled with alumina as given in the previous paper, order of adsorption, limit of detection, separation of several kinds of anions, and the method of microdetection of I^-, NO₂^-, and CN^- ions were examined. It was found that the separations of Cl^- and PO₄^---, Cl^- and AsO₄^---, PO₄^--- and AsO₄^----, AsO₃^--- and AsO₄^---, I^-, CrO₄^--, and PO₄^---, NO₂^- and CrO₄^--, were possible. The coloration reagents used were, besides the known AgNO₃ method, CuCl₂, Hg(NO₃)₂, FeCl₃, and benzidine. Chromatographic separation of metal complexes were also carried out by the same method and the separation of Cr and Pb, which was previously reported as being comparatively difficult was found to be possible by preparing a tartaric acid complex, while that of cobalt, nickel, and cadmium waa found to be possible by the ammonium complex method.

著者

安永 峻五 下村 脩

出版者

日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.74, no.1, pp.66-68, 1954

被引用文献数

2

---

Relationship between the pH of the electrolyte solution and the degree of mobility was examined with inorganic ions. Electrolyte used were sodium nitrate, potassium thiocyanate, and tartaric acid series, and the ions detected were Ba⁺⁺, Pb⁺⁺, Cu⁺⁺, Zn⁺⁺, Ni⁺⁺, Fe⁺⁺⁺, Br^-, CNS^-, AsO₄^---, CrO₄^--, Fe(CN)₆^---, NO₂^-, and BO₃^---. Except in the case of extremely acid or alkaline range, the effect of pH was found to be rather small in inorganic ions in general, both in cations and anions. However, in the case of amphoteric metals, such as zinc, they behave similar to amino acids. Use of complex salt-forming electrolyte in the case of metallic ions, the effect of pH was found to be extremely great. In the case of tartaric acid electrolyte, metals were found to move as a cation in the acid range of lower pH while they behaved as anions in a higher pH range, and there is a certain pH (isoelectric point) at which each metal does not move at all. From these facts, it was found that the complex salt-forming electrolyte was suitable for the separation of metals.

著者

安永 峻五 下村 脩

出版者

日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.73, no.12, pp.1346-1350, 1953

被引用文献数

6

---

A glass capillary of approximately 0.8mm. inside diameter and approximately 10cm. long, filled with alumina and having a pinhole at one end was used as a means of detecting several kinds of mixed ions by suction of sample solution and coloration reagents into the capillary through the pinhole. Limit of detection of metallic ions by various coloration reagents was examined and the present method was found to be far sharper than the paper spot test and the best suited for the detection of a minute amout of ions. The feature of this method may be said to lie in the fact that only a minute amount of sample and alumina are needed and that the procedure is simple and time short.

著者

安永 峻五 下村 脩

出版者

日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.73, no.12, pp.1353-1356, 1953

被引用文献数

3

---

By the determination of the stepwise change of pH in the absorption tube during cation adsorption and the amount of cerresponding anion adsorbed, precipitation of basic salt of metals was assumed, and following tests were carried out. Hydrogen ion concentration at the time of precipitation was determined with glass electrode pH meter and the relationship between pH at the time of absorption and the amount absorbed were examined by static adsorption, and it was found that the precipitation pH range and the order of adsorption were in good agreement and that the adsorption of cation became maximum in the precipitation pH range. From these facts, it was assumed that the adsorption of metallic ions by alumina was chiefly due to the precipitation of their basic salts. It was also found that the influence of anions was rather great in the adsorption of mercury salt, as the adsorption of mercury became extremely small in the presence of a chloride and this was assumed to be due to the formation of mercuric chloride of small degree of dissociation.

著者

朝比奈 泰彦

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.328, pp.626-643, 1909-06-26

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著者ハ本邦産延胡索科ノ一植物こまくさノ成分ヲ檢シ其ノ中ヨリ二種ノ植物鹽基ト一種ノ黄色無窒素結晶體ヲ抽出セリ植物鹽基ノ一ツハProsopinナルコトヲ證シ他ノ一ハ新シキ物質ニシテ著者ハコレヲDicentrinト命名シ其ノ集成ヲC_<20>H_<21>NO_4ト定メ各種ノ誘導體ヲ製シテコレヲ確證セリ、黄色結晶體ハC_<16>H_<12>O_7ナル集成ヲ有シ「ベルキン氏ノ「イソラムネチン」ト同一物ナルガ如シト

著者

三浦 孝次 池田 政男 大橋 冨次 岡田 いく子 五十嵐 良子

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.84, no.4, pp.341-345, 1964

被引用文献数

5

---

Antitumor activity of a large number of nitrofuran derivatives of quinoline was tested with mice bearing Ehrlich ascites tumor EY-33. Pure strain healthy <i>ddN</i> mice, weighing 18-22g., were intraperitoneally inoculated with this Ehrlich ascites tumor. After 24 hours, 0.2ml. of 5% glucose solution of the test compound was injected intraperitoneally, once a day for 7 days, to test antitumor activity. Normal mice inoculated with this ascites tumor generally died from accumulation of the ascites after about 10-19 days. Compounds were considered effective when the mice survived 50 days after the inoculation of the ascites. The compounds found to be effective by this test were sodium 2-[2-(5-nitro-2-furyl)vinyl]-4-quinolinecarboxylate (I), 2-[2-(5-nitro-2-furyl)-vinyl]-4-aminoquinoline lactate (II), and 4-[2-(5-nitro-2-furyl)vinyl]-2-aminoquinoline lactate (III). For the sake of comparison, panfuran hydrochloride and mitomycin-C were tested at the same time.<br>The data obtained with these compounds were as follows (given in the order of the name of compound, LD₅₀ in mg./kg., ED₆₀ in mg.&times;kg., and C. I.): I, 240, 20, 12; II, 23.8, 1, 23.8; III, 26.3, 5, 5.2; panfuran hydrochloride, 72.5, 0, 0; mitomycin-C, 5.2, 0.5, 12.4.<br>In order to clarify the action mechanism of these compounds, their action in suppressing the dehydronase of Ehrlich ascites tumor, and syntheses of nucleic acid and protein by <i>coli bacilli</i> was examined. It was presumed from its results that the antitumor action of I was due mainly to the suppression of dehydrogenase action, and that of II and III to the suppression of dehydrogenase action and DNA synthesis.

著者

太田 達男 宮崎 利夫

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.78, no.5, pp.538-539, 1958

被引用文献数

4

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From the air-dried pericarps of <i>Ruta graveolens</i> L., a trace of phenolic and nonphenolic weak bases (0.07%) were isolated by succesive extraction with petroleum ether and methanol, and subsequent treatment in the usual manner. The colorless needle crystals, m.p. 169-170&deg; (picrate: m.p. 217-218&deg;), obtained from the non-phenolic bases by crystallization from hydrated ethanol, were found to be identical with those of kokusaginine. The pale yellow prismatic crystals, m.p. 176&deg; (picrate: m.p. 195-197&deg;), obtained in 0.0018% yield from the foregoing crystallization mother liquor by chromatography on alumina in benzene solution, were proved to be those of skimmianine.

著者

田辺 良久

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.74, no.2, pp.162-167, 1954

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Syntheses of the Brooker-type dyes were reported in Part XV and XVI of this series, but some counter-argument has been made against the conclusion of the Brooker-type structure because of the difficulty of the mutual reaction of negative anilino groups and the possibility of another substitution in this synthetic method. The conclusion of the Brooker-type dyes was made from the analytical values and absorption spectra but further proof of the formation of a Brooker-type dye by the substitution of an anilino group in the <i>meso</i>-position was affored by the application of &omega;-phenylaminovinylbenzothiazolium ethiodide or &omega;-phenylamino-7-methylbenzothiazolium ethiodide on binuclear trimethinethiocyanine dye possessing inactive methyl in the terminal group and anilino group in the <i>meso</i>-position, to a Brooker-type thiocyanine with a terminal methyl, whose absorption spectrum was similar to or the same as that of binuclear trimethine dyes. It was also experimentally proved that reaction of negative anilino groups does occur by the fact that 1, 1&prime;-diethyl-2, 2&prime;-trimethinethiocyanine iodide was obtained from &omega;-phenylaminovinyl-benzothiazolium ethiodide and benzothiazole-2-aldehyde <i>p</i>-dimethylaminoanil ethiodide.

著者

伊藤 一男

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.81, no.5, pp.703-707, 1961

被引用文献数

3

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Presence of water-soluble quaternary bases was examined in the trunk bark of <i>Mechelia compressa</i> MAXIM. (Magnoliaceae) (Japanese name &lsquo;Ogatamano-ki&rsquo;) and a new base, named michepressine, was isolated as its iodide. Its chemical structure was established as <i>l</i>-1, 2-methylenedioxy-10-hydroxyaporphine (<i>l</i>-O-demethyllaureline methiodide), formulated as (VI).

著者

佐方 易忠 橋本 忠

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.79, no.7, pp.878-880, 1959

被引用文献数

3

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In order to examine chemical properties of <i>p</i>-trimethylsilylphenol (I), reaction of (I) with several kinds of cationoid reagents was carried out. Application of bromine to (I) affords <i>p</i>-bromophenol, while that of benzenediazonium chloride gives 4-hydroxyazobenzene. Nitration of acetate (II) of (I) with acetyl nitrate affords <i>p</i>-nitrophenyl acetate and the Friedel-Crafts reaction of the methyl ether (III) of (I) with anhydrous aluminum chloride and acetic anhydride gives <i>p</i>-methoxyacetophenone. The Fries rearrangement of (II) results in formation of <i>o</i>- and <i>p</i>-hydroxyacetophenone. The Reimer-Tiemann reaction of (I) affords 5-trimethyl-silylsalicylaldehyde. (I) is decomposed by 10% hydrochloric acid to form phenol but remains unchanged with 10% sodium hydroxide. These experimental results indicate that the cationoid reagents that easily undergo substitution at a position <i>para</i> to hydroxyl replaces the trimethylsilyl group in (I) while reagents that undergoes substitution in the <i>ortho</i> position does so in (I) without severance of a bond between silicon and the aromatic ring.

著者

藤田 直市 高橋 源三

出版者

公益社団法人 日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.60, no.6, pp.373-387, 1940-06-26 (Released:2009-10-21)