著者

松岡 佳子 鈴木 二郎

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.36, no.1, pp.83-86, 1987

被引用文献数

1

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スナネズミは, いろいろな分野で実験に用いられ, 大変有用な実験動物であるが, その繁殖, 発育に関する報告は少ない。筆者らは, 10年来, てんかん発作形質を選抜目標として, 近交系作出を試みている。その過程で得られた, 繁殖, 発育に関する成績は以下のとおりであった。1.平均寿命は, 雄26±14カ月, 雌27±13カ月であった。2.体重は, 16週齢でほぼ一定に達し, 雄で72±5.69, 雌で62±7.39であった。3.産仔数については, ばらつきがあるが, 平均4.8±1.7であった。4.分娩数は3~10月が多く, これらの時期が繁殖時期と考えられた。5.離乳率は, 約80%であった。6.世代別産仔数には大きな変動は見られなかった。<BR>本研究にあたり, 終始御協力下さった, 本研究所動物室, 岡崎守博, 赤坂佳幸の両氏ならびにタイプを打って下さった岩瀬真子さんに深謝する。

著者

Kanae Yasumatsu Jun-ichi Nagao Ken-ichi Arita-Morioka Yuka Narita Sonoko Tasaki Keita Toyoda Shoko Ito Hirofumi Kido Yoshihiko Tanaka

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.69, no.2, pp.250-260, 2020 (Released:2020-04-24)

参考文献数

33

被引用文献数

15

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Maternal immune activation (MIA) by an infection is considered to be an important environmental factor of fetal brain development. Recent animal model on MIA induced by polyinosinic:polycytidylic acid, a mimic of viral infection, demonstrates that maternal IL-17A signaling is required for the development of autism spectrum disorder (ASD)-like behaviors of offspring. However, there is little information on bacterial infection. In this study, we aim to elucidate the influence of MIA induced by lipopolysaccharide (LPS) to mimic a bacterial infection on fetal brain development. We demonstrated that LPS-induced MIA promoted ASD-like behaviors in mouse offspring. We further found that LPS exposure induced acute phase immune response: elevation of serum IL-17A levels in MIA mothers, upregulation of Il17a mRNA expression and increase of IL-17A-producing γδ T cells in the uterus, and upregulation of Il17ra mRNA expression in the fetal brain. Blocking of IL-17A in LPS-induced MIA ameliorated ASD-like behaviors in offspring. Our data suggest that bacterial-induced maternal IL-17A pathway promotes ASD-like behaviors in offspring.

著者

Luca Bordoni Eugenio Gutiérrez Jiménez Søren Nielsen Leif Østergaard Sebastian Frische

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.69, no.1, pp.92-103, 2020 (Released:2020-01-29)

参考文献数

58

被引用文献数

3

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The most used experimental mouse model of hyponatremia and elevated intracranial pressure (ICP) is intraperitoneal injection of water in combination with antidiuretics. This model of water intoxication (WI) results in extreme pathological changes and death within 1 h. To improve preclinical studies of the pathophysiology of elevated ICP, we characterized diuresis, cardiovascular parameters, blood ionogram and effects of antidiuretics in this model. We subsequently developed a new mouse model with mild hyponatremia and sustained increased ICP. To investigate the classical protocol (severe WI), C57BL/6mice were anesthetized and received an intraperitoneal injection of 20% body weight of MilliQ water with or without 0.4 µg·kg−1 desmopressin acetate (dDAVP). Corresponding Sham groups were also studied. In the new WI protocol (mild WI), 10% body weight of a solution containing 6.5 mM NaHCO3, 1.125 mM KCl and 29.75 mM NaCl was intraperitoneally injected. By severe WI, ICP and mean arterial pressure increased until brain stem herniation occurred (23 ± 3 min after injection). The cardiovascular effects were accelerated by dDAVP. Severe WI induced a halt to urine production irrespective of the use of dDAVP. Following the new mild WI protocol, ICP also increased but was sustained at a pathologically high level without inducing herniation. Mean arterial pressure and urine production were not affected during mild WI. In conclusion, the new mild WI protocol is a superior experimental model to study the pathophysiological effects of elevated ICP induced by water intoxication.

著者

庫本 高志 横江 繭子 矢ヶ崎 佳代子 KAWAGUCHI Tatsuya KUMAFUJI Kenta SERIKAWA Tadao

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.59, no.2, pp.147-155, 2010

被引用文献数

1 23

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To collect rat mutations and increase the value of the rat model system, we introduced fancy-derived mutations to the laboratory and carried out genetic analyses. Six fancy rats were shipped from a fancy rat colony in the USA and used as founders. After initial crosses with a laboratory strain, TM/Kyo or PVG/Seac, inbreeding started and 6 partially inbred lines, including 2 sublines, were produced as Kyoto Fancy Rat Stock (KFRS) strains. During inbreeding, we isolated 9 mutations: 5 coat colors, American mink (<i>am</i>), Black eye (<i>Be</i>), grey (<i>g</i>), Pearl (<i>Pel</i>), siamese (<i>sia</i>); 1 coat pattern, head spot (<i>hs</i>); 2 coat textures, Rex (<i>Re</i>), satin (<i>sat</i>); and an ear pinnae malformation, dumbo (<i>dmbo</i>). Genetic analyses mapped 7 mutations to particular regions of the rat chromosomes (Chr): <i>am</i> to Chr 1, <i>sia</i> to Chr 1, <i>sat</i> to Chr 3, <i>Re</i> to Chr 7, <i>g</i> to Chr 8, <i>dmbo</i> to Chr 14, and <i>hs</i> to Chr 15. Candidate gene analysis revealed that a missense mutation in the tyrosinase gene, Ser79Pro, was responsible for <i>sia</i>. From mutant phenotypes and mapping positions, it is likely that all mutations isolated in this study were unique to the fancy rat. These findings suggest that fancy rat colonies are a good source for collecting rat mutations. The fancy-derived mutations, made available to biomedical research in the current study, will increase the scientific value of laboratory rats.<br>

著者

Tamio OHNO Yuki MIYASAKA Masako KUGA Kaori USHIDA Miyoko MATSUSHIMA Tsutomu KAWABE Yoshiaki KIKKAWA Masashi MIZUNO Masahide TAKAHASHI

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.18-0185, (Released:2019-03-15)

被引用文献数

3

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Malaria is caused by Plasmodium parasites and is one of the most life-threatening infectious diseases in humans. Infection can result in severe complications such as cerebral malaria, acute lung injury/acute respiratory distress syndrome, and acute renal injury. These complications are mainly caused by P. falciparum infection and are major causes of death associated with malaria. There are a few species of rodent-infective malaria parasites, and mice infected with such parasites are now widely used for screening candidate drugs and vaccines and for studying host immune responses and pathogenesis associated with disease-related complications. We found that mice of the NC/Jic strain infected with rodent malarial parasites exhibit distinctive disease-related complications such as cerebral malaria and nephrotic syndrome, in addition to a rapid increase in parasitemia. Here, we focus on the analysis of host genetic factors that affect malarial pathogenesis and describe the characteristic features, utility, and future prospects for exploitation of the NC/Jic strain as a novel mouse model for malaria research.

著者

Jiyuan SI Ranran MENG Peng GAO Feifei HUI Yu LI Xianhu LIU Bin YANG

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.18-0089, (Released:2018-10-23)

被引用文献数

12

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Percutaneous coronary intervention (PCI) is main treatment for acute coronary syndrome (ACS). However, restenosis caused by PCI-induced injury influences the outcome of patients. Linagliptin, a dipeptidyl peptidase-4 (DPP-4) inhibitor, has been reported to ameliorate intimal hyperplasia post vascular injury. The underlying mechanisms by which linagliptin protects against balloon injury are unclear and require to be explored. Herein, Wistar rats with carotid artery balloon injury were given 1, 2 or 3 mg/kg/day linagliprin for 6 weeks. We found that linagliptin attenuated vascular injury-mediated neointima formation in rats without affecting body weight and blood glucose levels. ELISA results indicated that linagliptin significantly reduced overproduction of cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 post balloon injury. By detecting the level of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), we found that linagliptin prevented balloon injury-induced oxidative stress. Additionally, linagliptin decreased the level of Kelch ECH-associating protein 1 (KEAP1) compared with injury group. Results of Western blots and electrophoretic mobility shift assay (EMSA) demonstrated that linagliptin augmented nuclear accumulation of nuclear factor-E2-related factor 2 (NRF2) and its binding ability to target genes in rats with balloon injury. Moreover, heme oxygenase-1 (HO-1) and NAD(P)H quinine oxidoreductase 1 (NQO1), two downstream targets of NRF2, were further up-regulated after linagliptin treatment compared with injury group. In conclusion, our data suggest that linagliptin protects carotid artery from balloon injury-induced neointima formation and activates the NRF2 antioxidant pathway.

著者

Hao ZHANG Zhong-Li LI Xiang-Zheng SU Li DING Ji LI Heng ZHU

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.17-0137, (Released:2018-03-08)

被引用文献数

4

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Rabbit mesenchymal stem cells (MSCs) are important seed cells in regenerative medicine research, particularly in translational research. In the current study, we showed that rabbit subchondral bone is a reliable source of MSCs. First, we harvested subchondral bone (SCB) from the rabbit knee-joint and initiated the MSC culture by cultivating enzyme-treated SCB. Adherent fibroblast-like cells that outgrew from SCB fulfill the common immuno-phenotypic criteria for defining MSCs, but with low contamination of CD45+ hematopoietic cells. Interestingly, differentiated SCB-MSCs expressed osteogenic and chondrogenic markers at significantly higher levels than those in bone marrow cell suspension-derived MSCs (BMS-MSCs) (P<0.05). No differences in the expression of adipogenic markers between SCB-MSC and BMS-MSC (P>0.05) were observed. Moreover, the results of the colony forming unit-fibroblast assay and sphere formation assay demonstrated that the SCB-MSCs had increased self-renewal potential. SCB-MSCs expressed higher levels of the stemness markers Nanog, OCT4, and Sox-2 compared to in BMS-MSCs (P<0.05). Furthermore, the results of both the CCK-8-based assay and CFSE dilution assay showed that SCB-MSCs exhibited enhanced proliferative capacity. In addition, SCB-MSCs exhibited higher phosphorylation of extracellular signal-related kinase/mitogen-activated protein kinase signaling, which is closely related to MSC proliferation. In conclusion, we identified SCB-MSCs as a novel stem cell population that met the requirements of MSCs; the unique properties of SCB-MSC are important for the potential treatment of tissue damage resulting from disease and trauma.

著者

Akiyoshi Ishikawa Keita Sakai Takehiro Maki Yuri Mizuno Kimie Niimi Yasuhiro Oda Eiki Takahashi

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.66, no.1, pp.51-60, 2017 (Released:2017-01-27)

参考文献数

33

被引用文献数

19

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To understand sleep mechanisms and develop treatments for sleep disorders, investigations using animal models are essential. The sleep architecture of rodents differs from that of diurnal mammals including humans and non-human primates. Sleep studies have been conducted in non-human primates; however, these sleep assessments were performed on animals placed in a restraint chair connected via the umbilical area to the recording apparatus. To avoid restraints, cables, and other stressful apparatuses and manipulations, telemetry systems have been developed. In the present study, sleep recordings in unrestrained cynomolgus monkeys (Macaca fascicularis) and common marmoset monkeys (Callithrix jacchus) were conducted to characterize normal sleep. For the analysis of sleep–wake rhythms in cynomolgus monkeys, telemetry electroencephalography (EEG), electromyography (EMG), and electrooculography (EOG) signals were used. For the analysis of sleep–wake rhythms in marmosets, telemetry EEG and EOG signals were used. Both monkey species showed monophasic sleep patterns during the dark phase. Although non-rapid eye movement (NREM) deep sleep showed higher levels at the beginning of the dark phase in cynomolgus monkeys, NREM deep sleep rarely occurred during the dark phase in marmosets. Our results indicate that the use of telemetry in non-human primate models is useful for sleep studies, and that the different NREM deep sleep activities between cynomolgus monkeys and common marmoset monkeys are useful to examine sleep functions.

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.66, no.Supplement, pp.S23-S25, 2017 (Released:2017-06-10)

著者

Seiya MIZUNO Saori IIJIMA Tomoko OKANO Noriko KAJIWARA Satoshi KUNITA Fumihiro SUGIYAMA Ken-ichi YAGAMI

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.60, no.2, pp.161-167, 2011 (Released:2011-04-21)

参考文献数

14

被引用文献数

5 18

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We found 6 spontaneous mutant mice with long pelage hair in our ICR breeding colony. The abnormal trait was restricted to long hair in these mice, which we named moja. They were fertile and showed the same growth and behavior as wild-type mice. To investigate the manner of the genetic inheritance of the moja allele, offspring were bred by mating the moja mice; all offspring had long pelage hair. Furthermore, we performed a reciprocal cross between moja mice and wild-type ICR mice with normal hair. All offspring exhibited normal hair suggesting an autosomal recessive inheritance of the trait. The moja/moja hair phenotype was maintained in skin grafted onto nude mice, suggesting that circulating or diffusible humoral factors regulating the hair cycle are not involved in the abnormal trait. The phenotype of moja/moja mice is similar to that of Fgf5-deficient mice. Therefore, we examined the expression of Fgf5 by RT-PCR in moja/moja mice. As expected, no Fgf5 expression was found in moja/moja mouse skin. PCR and DNA sequence analyses were performed to investigate the structure of the Fgf5 gene. We found a deletion of a 9.3-kb region in the Fgf5 gene including exon 3 and its 5’ and 3’ flanking sequences. Interestingly, the genomic deletion site showed insertion of a 498-bp early transposon element long terminal repeat. Taken together, these results suggest that the long hair mutation of moja/moja mice is caused by disruption of Fgf5 mediated by insertion of a retrotransposon.

著者

Chihiro MORI Kazuhiro WADA

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.15-0008, (Released:2015-04-24)

被引用文献数

8

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Like humans, songbirds are one of the few animal groups that learn vocalization. Vocal learning requires coordination of auditory input and vocal output using auditory feedback to guide one’s own vocalizations during a specific developmental stage known as the critical period. Songbirds are good animal models for understand the neural basis of vocal learning, a complex form of imitation, because they have many parallels to humans with regard to the features of vocal behavior and neural circuits dedicated to vocal learning. In this review, we will summarize the behavioral, neural, and genetic traits of birdsong. We will also discuss how studies of birdsong can help us understand how the development of neural circuits for vocal learning and production is driven by sensory input (auditory information) and motor output (vocalization).

著者

Masashi HASHIMOTO Masayuki FUNABA Seinosuke OHSHIMA Matanobu ABE

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.44, no.1, pp.23-28, 1995 (Released:2003-12-23)

参考文献数

29

被引用文献数

3 5

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This study was conducted to examine the relationship between dietary nitrogen (N)-corrected metabolizable energy (MEn) and dietary digestible energy (DE) in cats, in order to verify the reliability of the present metabolizable energy (ME) system for cats. Four adult female cats were fed diets containing four different levels of crude protein (CP) (24, 35, 49, and 62% as fed) 4 hours a day in a 4 × 4 Latin square design to determine energy-and N-balance. Dietary CP levels had hardly any effect on daily food intake, but acid-ether extract (AEE) intake tended to increase and carbohydrate (CHO) intake tended to decrease, in response to increases in dietary CP levels. Apparent CP and AEE digestibility did not change, regardless of the experimental diet. In contrast, CHO digestibility tended to diminish as dietary CP levels increased. Although the ratio of urinary energy (UE) to urinary N (UN) was higher in cats fed the lowest CP diet, it was still much lower than in other mammals. Regression between UE/digestible crude protein (DCP) and N-balance indicated that dietary ME at N-equilibrium (i.e., MEn) could be expressed as DE -0.47 × DCP. MEn could also be estimated as DE -0.62 × DCP by using the average ratio of UE/(UN × 6.25). Both DCP coefficients were much lower than in other mammals, including dogs and pigs, suggesting a unique form of N metabolism in cats. Because ME values applied to practical feline feed ingredients have been either estimated in pigs or calculated according to the equation, DE -1.25 × DCP, similar to the method used for dogs, the present ME values for cats are believed to have been underestimated.

著者

Kazuyuki MEKADA Kuniya ABE Ayumi MURAKAMI Satoe NAKAMURA Hatsumi NAKATA Kazuo MORIWAKI Yuichi OBATA Atsushi YOSHIKI

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.58, no.2, pp.141-149, 2009 (Released:2009-05-16)

参考文献数

33

被引用文献数

76 270

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The C57BL/6 mouse is the most well-known inbred mouse strain, and has been widely used as a genetic background for congenic and mutant mice. A number of C57BL/6 substrains have been derived from the C57BL/6 founder line and are reported to differ in several phenotypes. There are several major sources of C57BL/6 substrains for the biomedical research community. The importance of their genetic and phenotypic differences among substrains, however, has not yet been well recognized by biomedical researchers. Here, we report the result of screening of the functional deletion of the nicotinamide nucleotide transhydrogenase (Nnt) gene and 1,446 SNPs genotyping among seven C57BL/6 substrains from different sources, such as C57BL/6J, C57BL/6JJcl, C57BL/6JJmsSlc, C57BL/6NJcl, C57BL/6NCrlCrlj, C57BL/6NTac, and C57BL/6CrSlc. The deletion of exon 7-11 in the Nnt gene that was previously reported in C57BL/6J was also observed in other C57BL/6J substrains, indicating that this functional deletion probably occurred at an early stage in the establishment of C57BL/6J substrains. The genotyping of SNP loci clearly demonstrate genetic differences between C57BL/6J and C57BL/6N substrains at 11 loci. Besides, we found another SNP differing between C57BL/6J and other C57BL/6J substrains available from commercial breeders. No genetic difference was detected among C57BL/6N substrains. The C57BL/6CrSlc mouse, originally derived from the National Cancer Institute of the NIH was found to be the same as the C57BL/6N substrains by the SNP pattern. These data will be useful for accurate genetic monitoring of genetically engineered mice with the C57BL/6 background.

著者

都築 政起 若杉 昇

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.37, no.2, pp.137-144, 1988-04-01 (Released:2010-08-25)

参考文献数

9

被引用文献数

3 3

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ニホンウズラの新しい突然変異「back-drawer」は, 腹部を覗き込むようにして首を腹側に強く曲げ, しゃがみ込んだ姿勢で, 常時後ずさりをする行動を示し, 時折前方に回転する。この異常は, 孵化時から8週齢の間に発症し, 症状は一時的な発作として出現するのではなく, 一定期間持続した。発症個体は3つのタイプに分けられ, 1つは, 孵化時既に症状を示しており5日齢以前に死亡するもので, 他の2つは2週齢時以降に発症し, 発症後早期に死亡するもの, および長期間生存するものであった。後者では, 症状が漸次軽減し, やがて正常個体との区別が困難となるものもあった。雄は, 外見的に性成熟に達した後も, 異常行動を示す期間は繁殖力をもたないが, 回復後は繁殖力をもつようになる。一方, 雌はほとんど産卵せず早期に死亡する傾向がある。後ずさり形質は, 常染色体性の2対の劣性遺伝子によって支配されていると考えられる。

著者

田嶋 嘉雄

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.33, no.1, pp.1-23, 1984-01-01 (Released:2010-08-25)

被引用文献数

1 1

著者

Tomoyuki SATO Tomoyo OCHIISHI Sayaka HIGO-YAMAMOTO Katsutaka OISHI

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.23-0104, (Released:2023-12-14)

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Disturbances in sleep-wake and circadian rhythms may reportedly precede the onset of cognitive symptoms in the early stages of Alzheimer’s disease (AD); however, the underlying mechanisms of these AD-induced sleep disturbances remain unelucidated. To specifically evaluate the involvement of amyloid β (Aβ) oligomers in AD-induced sleep disturbances, we examined circadian and sleep phenotypes using an Aβ-GFP transgenic (Aβ-GFP Tg) mouse characterized by intracellular accumulation of Aβ oligomers. The circadian rhythm and free-running period of wheel running activity were identical between Aβ-GFP Tg and littermate wild-type mice. The durations of rapid eye movement (REM) sleep were elongated in Aβ-GFP Tg mice; however, the durations of non-REM sleep and wakefulness were unaffected. The Aβ-GFP Tg mice exhibited shifts in the electroencephalogram (EEG) power spectra toward higher frequencies in the inactive light phase. These findings suggest that the intracellular accumulation of Aβ oligomers might be associated with sleep quality; however, its impact on circadian systems is limited.

著者

Mohammad Abdul AWAL Mohammad ASADUZZAMAN Mohammad Khairul ANAM Mohammad Abdul Aziz PRODHAN Masamichi KUROHMARU

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.50, no.4, pp.349-352, 2001 (Released:2003-11-06)

参考文献数

18

被引用文献数

1 1

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Arterial supply to the stomach of dogs indigenous to Bangladesh was investigated by using latex. The hepatic, left gastric and splenic arteries sent their major branches to the stomach. The cranial and caudal branches of the left gastric artery supplied the lesser curvature of the stomach. The right gastric, and right and left gastroepiploic arteries also sent their branches to both the lesser and greater curvatures. Six or seven short gastric arteries from the splenic artery supplied the greater curvature. Anastomoses between the left and right gastric, between the left and right gastroepiploic, and between short gastric arteries and left gastric arteries were observed.

著者

矢沢 肇 梅沢 英彦 倉益 茂実 宮嶋 正康

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.35, no.2, pp.203-206, 1986-04-01 (Released:2010-08-25)

参考文献数

6

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日本生物科学研究所付属実験動物研究所において全兄妹交配により, JWY-NIBS及びNWY-NIBSの2近交系が確立された。それらの起源, 確立過程, 成熟時月齢, 成熟時体重及び標識遺伝子は下記の如く要約される。1.JWY-NIBS: 起源: 東京都の高尾山付近で繁殖され, その後府中市の農家で維持されていたウサギを起源とする。近交開始年月: 1964年4月。近交系確立年月: 1981年6月。成熟時月齢: メス7カ月, オス7.5カ月。成熟時体重: メス2.8~3.0kg, オス2.7~2.9kg。生化学的標識遺伝子: ヘモペキシンHxs型, エステラーゼEst-1s型, α-プロテインはF型にそれぞれ固定していた。2.NWY-NIBS: 起源: 米国ジャクソン研究所より1967年に導入された系統III, ニュージーランドホワイト種を超源とする。近交開始年月: 1967年11月。近交系確立年月: 1982年7月。成熟時月齢: メス7.5カ月, オス8カ月。成熟時体重: メス2.8~3.0kg, オス2.9~3.1kg。生化学的標識遺伝子: ヘモペキシンHxF型。エステラーゼEst-1s型及びEst-2f型, α-プロテインはS型にそれぞれ固定していた。

著者

Yutaka MASUDA Minoru SUZUKI Yusuke AKAGAWA Takaubu TAKEMURA

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.48, no.3, pp.209-211, 1999 (Released:2003-11-22)

参考文献数

4

被引用文献数

9 9

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We demonstrated emotional piloerection in mice given conditioned fear stress by means of a pass-through apparatus. The emotional piloerection was first assessed in mice of different ages. The results showed that the piloerection changed with age. Pharmacological studies showed that the piloerection was inhibited by an alpha 1-adrenoceptor antagonist prazosin, but, surprisingly, was not inhibited by anxiolytic diazepam. These findings strongly suggest that the neuronal system of piloerection is different from that of freezing behavior, and that the neuronal system of piloerection develops with age.

著者

Michiko HIGASHI Saori TAHARABARU Yushi U. ADACHI Maiko SATOMOTO Takahiro TAMURA Naoyuki MATSUDA Aiji SATO-BOKU Masahiro OKUDA

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.23-0010, (Released:2023-06-02)

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Administration in a lipid emulsion can modify the pharmacodynamics of drugs via a process known as lipid resuscitation. However, the detailed mechanism remains unclear. We studied the volume and another pharmacodynamic effect, the lipid sink, using propofol and thiamylal. Male adult mice (ddY) were intravenously administered 10 mL/kg propofol or thiamylal diluted with physiological saline, 10% soybean oil, or 20% soybean oil. The 50% effective dose (ED50) for achieving hypnosis was calculated using probit analysis. To investigate the volume effect, 0, 10, or 20 mL/kg of saline or soybean oil was administered, either simultaneously or beforehand. Next, a two- or three-fold dose of the anesthetics was administered and the durations of anesthesia were measured. Finally, at 30 s after the first injection, supplemental soybean oil was administered. The mean (± standard error) ED50 values of propofol and thiamylal were 5.79 mg/kg (0.61) and 8.83 mg/kg (0.84), respectively. Lipid dilution increased the ED50 values of both anesthetics. After injection of a dose two-fold the ED50 value, the respective mean (± standard deviation) durations of anesthesia were 125 ± 35 s and 102 ± 38 s. Supplemental administration of soybean oil significantly shortened the duration of anesthesia of propofol, but not that of thiamylal. The results indicate that administration of a lipid emulsion vitiated the anesthetic effect of propofol by reducing the non-emulsified free fraction in the aqueous phase, which may elucidate the lipid resuscitation likely caused by the lipid sink mechanism.