著者

櫻井 小平太

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.111, pp.397-399, 1891-05-26

著者

橋本 庸平

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.15, no.4, pp.315-317, 1979-04-01

著者

大宮 茂

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.32, no.8, pp.961-962, 1996-08-01

著者

渡辺 一弘 岩田 一幸 丹代 優香 西澤 信 山岸 喬 吉沢 逸雄

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.38, no.3, pp.258-262, 1992-06-30

被引用文献数

11

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The effects of soluble sodium alginate of average molecular weight (A.M.W.), 1×10^4 (AG-1), 5×10^4 (AG-5) or 1×10^5 (AG-10) on the excretion of ^3H-labeled cholesterol, Trp-P-1 and aflatoxin B_1 in rat were compared with those of commercially available sodium alginate (A.M.W. : 2.7×10^6,AG-270) and polydextrose. In rats administered ^3H-cholesterol, the simultaneous administration of AG-10 or AG-270 (100mg/kg) significantly increased the amount of isotope excreted in feces. The administration of AG-5 or AG-10 (1000 mg/kg) after the administration of the tested compounds increased the amounts isotope excretion into feces, and decreased significantly those into urine. The administration of AG-1,however, showed no effect. These results indicate that AG-5 and AG-10 exhibited the same accelerating effects on the excretion of the tested compounds as AG-270.

著者

Young-Hoon Song Soo-Jin Jeong Hee-Young Kwon Bonglee Kim Sung-Hoon Kim Dong-Youl Yoo

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.35, no.7, pp.1022-1028, 2012-07-01 (Released:2012-07-01)

参考文献数

33

被引用文献数

13 39

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Although ursolic acid isolated from Oldenlandia diffusa (Rubiaceae) was known to have anticancer activities in prostate, breast and liver cancers, the underlying mechanism of ursolic acid in ovarian cancer cells was not investigated so far. In the present study, the apoptotic mechanism of ursolic acid was elucidated in SK-OV-3 ovarian cancer cells by 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay, cell cycle analysis and Western blotting. Ursolic acid exerted cytotoxicity against SK-OV-3 and A2780 ovarian cancer cells with IC50 of ca. 50 and 65 µM, respectively. Apoptotic bodies were observed in ursolic acid treated SK-OV-3 cells. Also, ursolic acid significantly increased ethidium homodimer stained cells and sub-G1 apoptotic portion in SK-OV-3 cells. Consistently, Western blotting revealed that ursolic acid effectively cleaved poly(ADP-ribose) polymerase (PARP), caspase-9 and -3, suppressed the expression of survival genes such as c-Myc, Bcl-xL and astrocyte elevated gene (AEG)-1, and upregulated phosphorylation of extracellular signal-regulated kinase (ERK) in SK-OV-3 cells. Interestingly, ursolic acid suppressed β-catenin degradation as well as enhanced phosphorylation of glycogen synthase kinase 3 beta (GSK 3β). Furthermore, GSK 3β inhibitor SB216763 blocked the cleavages of caspase-3 and PARP induced by ursolic acid and proteosomal inhibitor MG132 disturbed down-regulation of β-catenin, activation of caspase-3 and decreased mitochondrial membrane potential (MMP) induced by ursolic acid in SK-OV-3 cells. Overall, our findings suggest that ursolic acid induces apoptosis via activation of caspase and phosphorylation of GSK 3β in SK-OV-3 cancer cells as a potent anti-cancer agent for ovarian cancer therapy.

著者

Kensuke Yamamura Junichi Kitagawa Masayuki Kurose Shinichiro Sugino Hanako Takatsuji Rahman Md Mostafeezur Hossain Md Zakir Yoshiaki Yamada

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.33, no.11, pp.1786-1790, 2010-11-01 (Released:2010-11-01)

参考文献数

47

被引用文献数

14 39

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Swallowing involves several motor processes such as bolus formation and intraoral transport of a food bolus (oral stage) and a series of visceral events that occur in a relatively fixed timed sequence but are to some degree modifiable (pharyngeal stage or swallow reflex). Reflecting the progressive aging of society, patients with swallowing disorders (i.e., dysphagia) are increasing. Therefore, there is expanding social demand for the development of better rehabilitation treatment of dysphagic patients. To date, many dysphagia diets have been developed and are available commercially to help bring back the pleasure of mealtimes to dysphagia patients. Texture modification of food to make the food bolus easier to swallow with less risk of aspiration is one of the important elements in dysphagia diets from the viewpoint of safety assurance. However, for the further development of dysphagia diets, new attempts based on new concepts are needed. One of the possible approaches is to develop dysphagia diets that facilitate swallow initiation. For this approach, an understanding of the mechanisms of swallow initiation and identification of factors that facilitate or suppress swallow initiation are important. In this review, we first summarize the neural mechanisms of swallowing and effects of taste and other inputs on swallow initiation based on data mainly obtained from experimental animals. Then we introduce a recently established technique for eliciting swallowing using electrical stimulation in humans and our ongoing studies using this technique.

著者

Kyung-Ah Jung Tae-Chul Song Daeseok Han In-Ho Kim Young-Eon Kim Chang-Ho Lee

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.28, no.9, pp.1782-1785, 2005 (Released:2005-09-01)

参考文献数

27

被引用文献数

43 88

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It is currently accepted that the consumption of fruit-derived antioxidants such as vitamin C, carotenoids, and flavonoids provides a preventive effect against cardiovascular disease. The purpose of the present study was to investigate potential cardiovascular protective properties of aqueous and 70% ethanol extracts from kiwifruit by analyzing the antioxidative, antihypertensive, hypocholesterolemic, and fibrinolytic activities in vitro. Aqueous and 70% ethanol extracts at 50 mg/ml showed DPPH-radical scavenging activities of 72.31% and 70.75%, respectively. Total antioxidant activity in linoleic acid emulsion was 85—88% at 10 mg/ml and 96—98% at 50 mg/ml of kiwifruit extract. Inhibitory activities against angiogensin I-converting enzyme of kiwifruit extracts were 21—26% at 10 mg/ml and 46—49% at 50 mg/ml, and inhibitory activities on HMG-CoA reductase were 13—14% at 10 mg/ml and 19—30% at 50 mg/ml. Fibrinolytic activity of kiwifruit was also observed at a high concentration of 100 mg/ml in both aqueous and 70% EtOH extracts. Based on our results, kiwifruit have potential cardiovascular protective properties in vitro.

著者

澤田 光平

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.42, no.2, pp.153-155, 2006-02-01

被引用文献数

1

著者

三島 愛之助

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.341, pp.475-476, 1910-07-26

著者

Seong-Gyu Ko Seung-Hee Koh Chan-Yong Jun Chang-Gyu Nam Hyun-Su Bae Min-Kyu Shin

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.27, no.10, pp.1604-1610, 2004 (Released:2004-10-01)

参考文献数

28

被引用文献数

25 56

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We performed this study to understand the molecular basis underlying the antitumor effects of Saussurea lappa, Pharbitis nil, Plantago asiatica and Taraxacum mongolicum, which have been used for herbal medicinal treatments against cancers in East Asia. We analyzed the effects of these medicinal herbs on proliferation and on expression of cell growth/apoptosis related molecules, with using an AGS gastric cancer cell line. The treatments of Saussurea lappa and Pharbitis nil dramatically reduced cell viabilities in a dose and time-dependent manner, but Plantago asiatica and Taraxacum mongolicum didn't. FACS analysis and Annexin V staining assay also showed that both Saussurea lappa and Pharbitis nil induce apoptotic cell death of AGS. Expression analyses via RT-PCR and Western blots revealed that Saussurea lappa, but not Pharbitis nil, increased expression of the p53 and its downstream effector p21Waf1, and that the both increased expression of apoptosis related Bax and cleavage of active caspase-3 protein. We also confirmed the translocation of Bax to mitochondria. Collectively, our data demonstrate that Saussurea lappa and Pharbitis nil induce growth inhibition and apoptosis of human gastric cancer cells, and these effects are correlated with down- and up-regulation of growth-regulating apoptotic and tumor suppressor genes, respectively.

著者

安本

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.485, pp.618-619, 1922-07-26

著者

Tetsuo Watanabe Yuki Nakajima Tetsuya Konishi

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.31, no.1, pp.111-117, 2008-01-01 (Released:2008-01-01)

参考文献数

40

被引用文献数

8 18

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Basidiomycetes-X (BDM-X) is a novel edible mushroom recently identified as a new fungi species and registered to the database of the NPO organization for International Patent Organism Depositing (IPOD) in the Industrial Technology Institute of Japan (PCT/JP2004/006418). In the present study, we examined anti-oxidant activity of hot water extract of this novel fungus both in vitro and in vivo together with Agaricus Blazei Murill (ABM), a commercially available medicinal mushroom, and other reference antioxidants. As results, the hot water extract of BDM-X showed more potent anti-oxidative actions compared with that of ABM, when evaluated by 1,1′-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, Ferric Reducing Potential (FRP), and also the inhibition of 2,2′-azobis(2-amidino-propane) dihydrochloride (AAPH)-induced lipid peroxidation of rat liver homogenates. Further, the protective activity of BDM-X extract towards lipopolysaccharide (LPS)-induced hepatic oxidative damage was compared with ABM and α-lipoic acid in the mice pre-administered with these antioxidants. It was revealed that all of these antioxidants inhibited the LPS-induced oxidative tissue damage but the hot water extract of BDM-X showed the strongest protection among them. For example, the dose for 50% inhibition of carbonyl formation in liver was 0.53 g dry weight/kg body weight/d for BDM-X and the value corresponded to 16 mmol of α-lipoic acid as an antioxidant reference/kg body weight/d.

著者

Katsuhiro YAMASAKI Masami NAKANO Takuya KAWAHATA Haruyo MORI Toru OTAKE Noboru UEDA Isao OISHI Rie INAMI Megumi YAMANE Miki NAKAMURA Hiroko MURATA Tsutomu NAKANISHI

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.21, no.8, pp.829-833, 1998-08-15 (Released:2008-04-10)

参考文献数

14

被引用文献数

75 158

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The anti-HIV-1 activity of aromatic herbs in Labiatae was evaluated in vitro. Forty five extract from among 51 samples obtained from 46 herb species showed significant inhibitory effects against HIV-1 induced cytopathogenicity in MT-4 cells. In particular, the aqueous extracts of Melissa officinalis, a family of Mentha×piperita "grapefruit mint", Mentha×piperita var. crispa, Ocimum basilicum cv "cinnamon", Perilla frutescens var. crispa f.viridis, Prunella vulgaris subsp. asiatica and Satureja montana showed potent anti-HIV-1 activity (with an ED of 16μg/ml). The active components in the extract samples were found to be water-solubel polar substances, not nonpolar compounds such as essential oils. In addition, these aqueous extracts inhibited giant cell formation in co-culture of Molt-4 cels with and without HIV-1 infection and showed inhibitory activity against HIV-1 reverse transcriptase.

著者

西川 眞弓 中島 邦生 五十嵐 一雄 糟谷 史代 福井 巳芳 土橋 均

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.38, no.2, pp.121-126, 1992-04-30

被引用文献数

8

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An analytical procedure for morphine-3-glucuronide (M3G) and morphine (M) in the human urine was investigated by using high-performance liquid chromatography coupled with atomospheric pressure ionization mass spectrometry (LC/APCI-MS). The samples were purified with Sep-Pak C_<18> cartridges. The LC separation was carried out using a L-column ODS in 50 mM ammonium acetate-methanol (86 : 14 v/v). The calibration graphs constructed by the absolute calibration curve method showed linearity over the concentration range of 30 to 1000 ng/ml (γ=0.9965) for M3G and 30 to 2000 ng/ml (γ=0.9970) for M. The detection limits by selected ion monitoring (SIM) were 3 ng/ml for M3G and 1 ng/ml for M, and by scan mode were 350 ng/ml for M3G and 80 ng/ml for M. The coefficients of variations for M3G and M were 4.79 and 3.15% at 1 μg/ml, respectively.

著者

四ツ柳 智久

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.23, no.7, pp.720-721, 1987-07-01

著者

Shuso Takeda

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.37, no.9, pp.1435-1438, 2014-09-01 (Released:2014-09-01)

参考文献数

43

被引用文献数

4 19

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Δ9-Tetrahydrocannabinol (Δ9-THC), a biologically active constituent of marijuana, possesses a wide variety of pharmacological and toxicological effects (e.g., analgesia, hypotension, reduction of inflammation, and anti-cancer effects). Among Δ9-THC’s biological activities, its recognized anti-estrogenic activity has been the subject of investigations. Since Δ9-THC is used as both a drug of abuse (marijuana) and as a preventive therapeutic to treat pain and nausea in cancer patients undergoing chemotherapy in the United States and other countries (synthesized Δ9-THC; dronabinol), it is important to investigate the mechanistic basis underlying the anti-estrogenic activity of Δ9-THC. Since Δ9-THC has “no” binding potential for estrogen receptor α (ERα) which can be activated by estrogen (E2), the question of how Δ9-THC exerts its inhibitory effect on ERα is not resolved. We have recently reported that ERβ, a second type of ER, is involved in the Δ9-THC abrogation of E2/ERα-mediated transcriptional activity. Here we discuss the possible mechanism(s) of the Δ9-THC-mediated disruption of E2/ERα signaling by presenting our recent findings as well.

著者

守安 貴子 重岡 捨身 岸本 清子 石川 ふさ子 中嶋 順一 上村 尚 安田 一郎

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.121, no.10, pp.765-769, 2001-10-01

被引用文献数

13 35

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A substantially available identification system for Sildenafil in health foods was established using 3 different analytical mathods; i.e.TLC, preparative TLC/MS and HPLC/photo-diode array. Sildenafil in health foods was extracted with ethyl acetate under alkaline conditions as sample solutions for TLC and preparative TLC, and also extracted with 50% methanol and then diluted with solution of HPLC mobile phase for HPLC. The sample solution for TLC was applied to Silica gel 60 F_<254> plates with chloroform/methanol/28% ammonia(90:1:5, under layer)as mobile phase. Spots were located under UV radiation at 254 nm and 366 nm, and spraying dragendorff reagent. The conditions for preparative TLC were the same as these of TLC method, samples abtained from preparative TLC were determined by MS with APCI interface, under both positive and negative modes. The HPLC analysis was carried out on a column of Cosmosil 5C18-AR(4.6mm×150mm, 5μm)with 0.05 mol/l phosphate buffer pH 3.0/acetonitrile(73:27)as mobile phase and the eluate was monitored by a photo-diode array detector. The quantitative analysis was available, when the peak of this sample on HPLC was detected at 290nm. When this system was applied to commercial health foods, Sildenafil was identified and their contents were 25 mg-45 mg/tablet or bottle. These contents nearly correspond to that in Viagra, 25 mg, 50 mg/tablet. Therefore, there is a fear of side effects for Sildenafil, when it is taken as health foods.

著者

慶松 勝左衞門 横田 嘉右衞門

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.510, pp.629-636, 1924-08-26

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Zur Darstellung der 2-Oxy-3.5-dinitrophenylarsinsaure haben die Verfasser die Methode von Hans Schmidt (Ann. 421,159), sowie von Bart (Ann. 429,57) benutzt, indem sie Pikraminsaure diazotirten und auf das Produkt in der alkalischen Losung arsenige Saure einwirken liessen. Die so erhaltene Substanz stellte gelbe Blattchen vom Smp. 237°. As-Gehalt (nach Lehmann) Gef. 24.35%, berech. f. C_6H_5O_8N_2As 24.34%. Diese ist identisch mit der 2-Oxy-3.5-dinitrophenylarsinsaure von Benda (Ber 44,3294). Reducirt man die letztere in der Kalte mit Natriumhydrosulfit, so wird eine Aminoverbindung gebildet, aber kein Arsenobenzolderivat. Behandelt man dagegen 2-Oxy-3.5-dinitropheny ars nsaure bei 60° kurze Zeit mit Hypophosphitlosung, so bleiben die Nitrogruppen intakt und wird Dioxytetranitroarsenobenzol gebildet. As-gehalt 28.95%, ber. f. C_<12>H_6O_<10>N_4As_2 29.05%. Lasst man die Hypophosphitlosung langere Zeit einwirken, so wird Dioxy-diamino-dinitroarsenobenzol (gef. As. 28.38%, ber. f. C_<12>H_<10>O_6N_4As_2・2HCl 28.34%) gebildet. Die Dioxytetraaminoarsenobenzol wurde jedoch nicht isolirt.

著者

北村 二朗

出版者

公益社団法人日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.17, no.5, pp.427-428, 1981-05-01

著者

Mamoru Tanaka Akihiro Tanaka Katsuya Suemaru Hiroaki Araki

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.36, no.2, pp.222-227, 2013-02-01 (Released:2013-02-01)

参考文献数

23

被引用文献数

1 3

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Antithrombotic drugs have been increasingly used for treating ischemic cardiovascular diseases among the elderly in Japan. However, antithrombotic drugs are known to be risk factors for gastrointestinal injury. Therefore, we conducted a pharmacoepidemiologic study on patients receiving antithrombotic drugs to identify the risk factors for gastrointestinal injury. This retrospective case-control study included patients who were prescribed antithrombotic drugs at the Ehime University Hospital between April and September 2010. Of the 3271 patients who received antithrombotic drug therapy, 172 (5.3%) developed gastrointestinal injuries, including gastric ulcers, duodenal ulcers, and hemorrhagic gastrointestinal injuries. Further, the incidence of gastrointestinal injury was higher in patients with hypertension than in those without (p<0.0001). Multivariate adjusted odds ratios and 95% confidence intervals were calculated using stepwise logistic regression. The adjusted odds ratios for gastrointestinal injury were 1.56 (95% confidence interval 1.07–2.36) for hypertension, 1.70 (1.17–2.50) for low-dose aspirin, 2.77 (1.70–4.49) for clopidogrel, 1.95 (1.23–3.08) for warfarin, and 4.13 (2.88–5.95) for nonsteroidal anti-inflammatory drugs. On the other hand, the non-adjusted odds ratio for drug-associated gastrointestinal injury was 0.43 (0.20–0.84) for eicosapentaenoic acid (EPA). In addition, we found that patients aged 70 years or older were at increased risk of drug-associated gastrointestinal injury. These findings suggest that while many antithrombotic drugs are risk factors for gastrointestinal injury, EPA may be a safe option for suppressing or preventing gastrointestinal injury.