著者

辻 保宏 掛川 寿夫 宮高 透喜 松本 仁 佐藤 利夫

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.16, no.7, pp.675-678, 1993-07-15 (Released:2008-04-10)

参考文献数

19

被引用文献数

1 2

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Formulations consisting of egg albumin, indomethacin (IND), and olive oil or fatty acids, were prepared by vigorous stirring using a high-speed homogenizer and subsequent freeze-drying. To confirm the anti-inflammatory properties and ulcerogenic effects of the formulatios, we examined the action of the formulations on carrageenan-induced edema in rats as well as their ulcerogenic actions in the same species. Compared with IND alone, albumin-IND-olive oil (9 : 1 : 4.3), albumin-IND-linolenic acid (9 : 1 : 4.3), albumin-IND-liolic acid (9 : 1 : 4.3), albumin-IND-oleic acid (9 : 1 : 4.3), albumin-IND-stearic acid (9 : 1 : 4.3), and albumin-IND-tristearin (9 : 1 : 4.3) formulations all exhibited a more potent inhibitory effect on carrageenan-induced edema. In addition, the inhibitory effects on edema formation of an albumin-IND (9 : 1) complex was as strong as that of IND alone. These results suggested that the biovailability of IND was increased by olive oil, fatty acid, and tristearin as absorbefacient agent. The increase in the bioavailability was evident from the fact that the mean plasma levels, maximum plasma levels (Cmax), and area under plasma concentration-time curve (AUC) values after oral administration of the albumin-IND-olive oil (9 : 1 : 4.3) formulation was significantly greater than that after administration of the drug alone. With respect to their ulcerogenic properties, the formulations were significantly less active than IND alone, suggesting that a reduction in the ulcerogenic activity of IND was by produced complexation with egg albumin.

著者

Fatema Tuj Zohra Yoshie Maitani Toshihiro Akaike

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.35, no.1, pp.111-115, 2012-01-01 (Released:2012-01-05)

参考文献数

35

被引用文献数

2 16

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It was believed for a long time that mRNA is very unstable, and can not be used for therapeutic purposes. In the last decade, however, many research groups proved its transfection feasibility along with advantages and applications. Our investigation is aimed at establishing a potent and efficient mRNA delivery system. We previously reported that an inorganic–organic hybrid carrier by exploiting the advantages of inorganic nano apatite particles onto organic carrier DOTAP {N-[1-(2,3-dioleoloxy)propyl]-N,N,N-trimethyl ammonium chloride} and showed potential effect of carbonate apatite particles on each of the mRNA delivery steps in dividing and non-dividing cell. Here, we report on the development of a more efficient mRNA carrier by complexing ECM protein, fibronectin with the DOTAP-apatite carrier. The carrier showed enhanced uptake of luciferase mRNA both qualitatively and quantitatively. Accelerated cellular endocytosis rate was evaluated using labeled endosome. Finally expression of lucifearse mRNA was higher for fibronectin complexed carrier in compared to the uncoated one.

著者

櫻井 小平太

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.52, pp.232-239, 1886-06-26

著者

Osamu Nakajima Kosuke Nakamura Kazunari Kondo Hiroshi Akiyama Reiko Teshima

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.36, no.9, pp.1454-1459, 2013-09-01 (Released:2013-09-01)

参考文献数

14

被引用文献数

2 3

---

Genetically modified (GM) chickens carrying the human erythropoietin (hEpo) gene have been developed to produce recombinant hEpo protein in eggs. However, such animals have not been approved as food sources in Japan. We developed a method for detecting the hEpo gene in chicken meat using a real-time polymerase chain reaction (real-time PCR). The hEpo gene was clearly detected in genomic DNA extracted from magnum and heart of a chimeric chicken containing the hEpo gene. A plasmid containing the hEpo gene was used as a standard reference molecule as well. The results clearly showed that our method was capable of detecting the hEpo gene contained in the plasmid in the presence of genomic DNA extracted from a raw chicken meat sample. We successfully used this method to test six samples of raw chicken meat and six samples of chicken meat in processed foods. This method will be useful for monitoring chicken meat that might have originated from GM chickens carrying the hEpo gene to assure food safety.

著者

相本 太刀夫 平田 巳円男 平木 優子 土手 千恵子 井上 直人 今井 公江

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.114, no.2, pp.89-93, 1994-02-25

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The antioxidative effect of the mammalian liver hydrolyzate preparation (LH) was investigated mainly in the mouse liver homogenates. At lower concentrations, LH stimulated lipid peroxidation induced by Fe^<2+> and ascorbic acid in the homogenates, but inhibited completely the oxidation at higher concentrations. LH also inhibited the peroxidation of linolenic acid and linoleic acid. LH reduced 1,1-diphenyl-2-picrylhydrazyl, a free radical model, in a concentration-dependent manner, suggesting that the radical scavenging property of LH is involved in its antioxidative action. Fe^<2+> and glutathione, contained in LH, seem to act as a stimulator and an inhibitor of peroxidation, respectively.

著者

Tomohiro Asai Naoto Oku

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.37, no.2, pp.201-205, 2014-02-01 (Released:2014-02-01)

参考文献数

30

被引用文献数

8 14 8

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Gene silencing mediated by RNA interference (RNAi) is expected to have a beneficial impact on the treatment of many diseases because of its potency, selectivity and versatility. To maximize the potential of RNAi effectors such as small interfering RNA and microRNA in clinical therapy, the development of a practical delivery system is required, especially for systemic administration. Recent studies demonstrated that chemical modification of these small RNAs and/or encapsulation of them into lipid nanoparticles is a promising strategy to achieve targeted delivery via systemic administration. In this review article, we introduce recent progress of the research on systemic delivery systems for RNAi therapeutics and consider crucial elements for the design of lipid nanoparticles as a small RNA vector.

著者

中村 清 宇津井 雄三 二之宮 靖子

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.86, no.5, pp.404-409, 1966-04-25

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Examination were made on gas chromatographic detection of approximately sixty kinds of nitrofuran derivatives. With the exception of a few compounds, majority of these derivatives were found to be detectable without decomposition.

著者

Gon Sup Kim Dong Hyeok Kim Jeong Ju Lim Jin Ju Lee Dae Yong Han Whi Min Lee Won Chul Jung Won Gi Min Chung Gil Won Man Hee Rhee Hu Jang Lee Suk Kim

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.31, no.11, pp.2012-2017, 2008-11-01 (Released:2008-11-01)

参考文献数

44

被引用文献数

23 55

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Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans. Recently, the emergence of multidrug-resistant strains of Salmonella sp. has caused more serious problems in public health. The present study investigated the antibacterial effects of Houttuynia cordata water extract (HCWE) against murine salmonellosis. In RAW 264.7 cells, there was no detectable cytotoxic effect of HCWE at any concentration between 25 and 100 μg/ml after 8-h incubation. The antibacterial activity of HCWE was then examined in a Salmonella enterica serovar (Salmonella typhimurium), and was found to increase in a dose-dependent manner at concentrations from 25 to 100 μg/ml during 8-h incubation. HCWE also affected RAW 264.7 cells including morphologic change and bacterial uptake, but there was no significant difference in bacterial replication in RAW 264.7 cells. With HCWE alone, nitric oxide (NO) production by RAW 264.7 cells did not increase, but when RAW 264.7 cells were infected by S. typhimurium, with or without HCWE, NO production with HCWE was 2-fold higher than that without HCWE. Treatment with HCWE did not affect inducible NO synthase (iNOS) mRNA expression by RAW 264.7 cells, but when RAW 264.7 cells with HCWE were infected by S. typhimurium, iNOS mRNA expression was increased during 8-h incubation. Furthermore, HCWE showed virulence reduction effects in S. typhimurium-infected BALB/c mice. After a lethal dose of S. typhimurium, the mortality rate in the HCWE untreated group was 100% at 7 d, but the HCWE 25, 50, and 100 μg/ml groups survived until 11, 17, and 23 d, respectively. These data suggest that HCWE is stable and beneficial in the treatment of bacterial infection including intracellularly replicating pathogens and may solve antimicrobial misuse and overuse.

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.17, pp.677-678, 1883-06-26

著者

佐藤 卓 池田 英男 古川 一 村田 雄司 供田 真由子

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.124, no.10, pp.705-709, 2004-10-01

被引用文献数

1 3

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カンゾウ(Glycyrrhiza glabra Linn.)は甘味剤, 胃腸薬などとして用いられる重要な生薬で, 日本における消費量は増加傾向にあるが, その大部分を海外, 特に中国からの輸入に頼っている. 2000年の日本のカンゾウ総輸入量は4151tで, このうち中国産は3250tを占め, 中国からの輸入は過去10年で最高となっている(厚生労働省, 2002). その一方で, カンゾウの乱獲による環境破壊が中国西北地域一帯で進行しつつあり, 中国政府は現在まで実施していたカンゾウの輸出許可制度を活用した輸出総量規制, 輸出港の限定による無許可輸出の防止, 輸出許可取得料の値上げ, 生産地に対する管理, 規制などを進めている. そこで, 中国では野生カンゾウの採集に代わり, 栽培によるカンゾウの生産を実施しているが, かならずしも日本薬局方の定めるグリチルリチン酸含有量を満たしていない. 一方, 個人輸入業者が主として販売する未承認医薬品による健康被害があとを絶たないことから, 既存の漢方や生薬においてもより厳密な品質管理が求められる可能性が考えられる.

著者

佐渡 正四郎

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.4, no.1, pp.16-19, 1957-02-25

著者

高木 修造 山木 正枝 弓岡 栄三郎 西村 正 先名 淳

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.102, no.4, pp.313-317, 1982-04-25

被引用文献数

1

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A new bitter principle, centauroside was obtained from the whole plant of Erythraea centaurium (LINNE) PERSOON. with two glucosides. On the basis of chemical and spectral evidence, the structure of centauroside was confirmed to be a novel bis-secoiridoidal structure (III), and the others were established to be 6'-(m-hydroxybenzoyl) loganin, and secologanin dimethyl acetal arising from secologanin during the extraction process.

著者

Intan Safinar Ismail Hideyuki Ito Teruo Mukainaka Hiroshi Higashihara Fumio Enjo Harukuni Tokuda Hoyoku Nishino Takashi Yoshida

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.26, no.9, pp.1351-1353, 2003 (Released:2003-09-01)

参考文献数

23

被引用文献数

13 24

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After bioassay-guided fractionation of the extract from Sandoricum koetjape bark, which exhibited significant toxicity to killifish (Oryzias latipes), two ichthyotoxic triterpenoids were isolated and characterized as koetjapic acid and 3-oxo-olean-12-en-29-oic acid. These constituents, along with non-toxic katonic acid, had a remarkable inhibitory effect on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol 13-acetate (TPA), which is a preliminary in vitro screening method for possible anti-tumor-promoting agents. Of the triterpenoids active in vitro, koetjapic acid appears to be a promising cancer chemopreventive agent, since it significantly delayed tumor promotion in two-stage mouse skin carcinogenesis induced by 7,12-dimethylbenz(a)anthracene and promoted by TPA.

著者

佐々木 功 藤田 卓也 村上 正裕 山本 昌 中村 英次 今崎 一 村西 昌三

出版者

公益社団法人日本薬学会

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.17, no.9, pp.1256-1261, 1994-09-15

被引用文献数

13 18

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Absorption of azetirelin, a new thyrotropin-releasing hormone (TRH) analogue, from the gastrointestinal (GI) tract was evaluated. The bioavailability of this compound after oral administration was considerably poor in rats. Studies were undertaken to elucidate the mechanisms for this low oral bioavailability of azetirelin. The plasma azetirelin levels following intravenous and hepatoportal vein injection were virtually identical over the dose range of 0.02-0.1 mg/kg, indicating a minor contribution of the hepatic first-pass metabolism of this drug. Azetirelin was stable against peptide hydrolases both in luminal fluid and intestinal mucosal homogenates, whereas its degradation occurred when incubated with cecal contents under an anaerobic condition. In addition, complete degradation of azetirelin during the GI transit was disclosed by analyzing the fecal sample collected after oral administration of [^<14>C] azetirelin. These results suggested that gut bacteria may be responsible for the hydrolysis of azetirelin in the GI tract. The low intestinal permeability of azetirelin was revealed by a modified everted gut experiment in various segments of the rat intestine. The poor membrane transport characteristics of azetirelin may be due to its high hydrophilicity. From these results, it was suggested that the insufficient oral bioavailability of azetirelin may be mainly attributed to its low intestinal permeability due to a lack of lipophilicity, and also to the degradation of the peptide by intestinal microflora.

著者

藤本

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.215, 1900-01-26

著者

Selvaraj SARAVANAN Ramasundaram SRIKUMAR Sundaramahalingam MANIKANDAN Narayanaperumal JEYA PARTHASARATHY Rathinasamy SHEELA DEVI

出版者

公益社団法人日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.127, no.2, pp.385-388, 2007 (Released:2007-02-01)

参考文献数

24

被引用文献数

17 46

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Hypercholesteremia is one of the risk factors for coronary artery disease. The present study highlights the efficacy of Ayurvedic herbal formulation Triphala (Terminalia chebula, Terminalia belerica, and Emblica officinalis) on total cholesterol, Low density lipoprotein (LDL), Very low density lipoprotein (VLDL), High density lipoprotein (HDL) and free fatty acid in experimentally induced hypercholesteremic rats. Four groups of rats were employed namely control, Triphala treated, hypercholesterolemia rats (4% Cholesterol+1% cholic acid+egg yolk) and Triphala pre-treatment in hypercholesteremic rats. Results showed significant increase in the total cholesterol, LDL, VLDL, and free fatty acid in hypercholesteremic rats were significantly reduced in Triphala treated hypercholesteremic rats. The data demonstrated that Triphala formulation was associated with hypolipidemic effects on the experimentally induced hypercholesteremic rats.

著者

三浦 俊明 村岡 早苗 小木曾 健人

出版者

公益社団法人日本薬学会

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.40, no.3, pp.709-712, 1992-03-25

被引用文献数

6

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Semiquinone radicals of menadione were generated during the reaction of menadione with reduced glutathione (GSH), dependent upon the pH. Under aerobic conditions, cytochrome c was reduced during the reaction, and superoxide dismutase (SOD) inhibited the cytochrome c reduction. The inhibitory effect of SOD was greater at a high pH than at a low pH. In the presence of Fe^<3+> or ethylenediaminetetraacetic acid (EDTA)-Fe^<3+>, deoxyribose was degraded during the reaction of menadione with GSH, dependent upon the pH. Greater amounts of deoxyribose were degraded at a low pH than at a high pH. The reduction of Fe^<3+> of EDTA-Fe^<3+> also depended on the pH, and SOD strongly inhibited the Fe^<3+> reduction, indicating that Fe^<3+> or Fe^<3+>-EDTA was reduced by superoxide. SOD, catalase, mennitol and benzoate inhibited the deoxyribose degradation at various pH values. These results indicate that the menadione semiquinone radical is readily formed at an alkali pH but a hydroxyl radical is predominantly produced near a neutral pH. A hydroxyl radical may be generated via an iron-catalyzed Haber-Weiss reaction.

著者

関屋 実 鈴木 次郎 柿谷 芳宏

出版者

公益社団法人日本薬学会

雑誌

Chemical & pharmaceutical bulletin (ISSN:00092363)

巻号頁・発行日

vol.18, no.6, pp.1233-1238, 1970-06-25

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Reaction for preparation of a new series of 5-acylaminooxazole-4-carboxamides has been found. 5-Acetamidooxazole-4-carboxamides were obtained by heating 2-acylamino-2-cyanoacetamides with acetic anhydride in the presence of perchloric acid. Analogous 5-form-amido derivatives were also obtained by heating the same substrates with a mixture of formic acid and acetic anhydride in the presence of hydrochloric acid. Trials for synthesis of oxazolo [5,4-d] pyrimidine-7-ol from the 5-acylaminooxazole-4-carboxamide obtained were also successful in some cases by means of heating with potassium bicarbonate solution.

著者

金尾

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

no.484, 1922-06-26

著者

杉原 明

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.86, no.4, pp.349-352, 1966-04-25

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It was found that 2-[2-(5-nitro-2-furyl) vinyl]-5-amino-1,3,4-oxadiazole (II) is obtained by the reaction of thiosemicarbazide with 3-(5-nitro-2-furyl) acryloyl chloride in excess pyridine and by refluxing 5-nitro-2-furanacrylic acid hydrazide with potassium thiocyanate in aqueous hydrochloric acid. 2,2-Disubstituted 3-acetyl-5-[2-(5-nitro-2-furyl) vinyl]-1,3,4-oxadiazolines (VIa〜c) were prepared by refluxing alkanone 5-nitro-2-furanacrylic acid hydrazones with acetic anhydride. Antimicrobial activities of these nitrofuran compounds are presented in Table III.