著者

Hiroyuki Wakiguchi Yasuhiro Okamoto Manaka Matsunaga Yuichi Kodama Akinori Miyazono Shunji Seki Naohiro Ikeda Yoshifumi Kawano

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.69, no.5, pp.424-425, 2016 (Released:2016-09-21)

参考文献数

10

被引用文献数

2 4

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Cat scratch disease (CSD) is an infectious disease caused by Bartonella henselae. Atypical clinical presentations of CSD include prolonged fever and multiple hepatosplenic lesions. Furthermore, multiple renal lesions are extremely rare in CSD. An 11-year-old Japanese girl presented at our hospital with a prolonged fever of unknown cause after being scratched and bitten by a kitten. Abdominal computed tomography (CT) revealed multiple small, round hypodense lesions in both kidneys and the spleen. Based on her history and the CT results, her diagnosis was CSD. The diagnosis was confirmed by serological tests, which indicated antibodies against B. henselae. After treatment with azithromycin, her fever immediately improved. Careful history taking and imaging are essential for the diagnosis of atypical CSD. In CT images, not only hepatosplenic lesions but also renal lesions are important features indicative of a diagnosis of atypical CSD. Subsequently, a diagnosis of CSD can be confirmed by specific serological tests. This is the first reported Japanese case of multiple renal and splenic lesions in a patient with CSD. Although difficult to diagnose, an early diagnosis atypical CSD and appropriate treatment are important to prevent complications and the need for invasive examinations.

著者

Larissa Moraes dos Santos Fonseca Rejane Hughes Carvalho Antonio Carlos Bandeira Silvia Ines Sardi Gubio Soares Campos

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2019.296, (Released:2019-11-29)

参考文献数

15

被引用文献数

10

---

Oropouche Virus (OROV) is a single-stranded negative-sense RNA arbovirus transmitted to humans by the midge Culicoides paraenesis causing a disease known as Oropouche fever. In Brazil, outbreaks of the virus have been reported to be restricted to the Central-Northern region of the country. However, its incidence is underestimated mainly due to the virus clinical similarities with other arbovirus diseases including dengue (DENV), chikungunya (CHIKV), and zika (ZIKV), and the lack of specific diagnostic tests. Here, we report for the first time OROV detection in saliva and urine samples and the first cases of OROV autochthone infections in Metropolitan region of Salvador, Bahia a Northeastern capital in the coast of Brazil. Serum, saliva and urine samples negative for DENV, CHIKV, and ZIKV were tested for OROV using a RT-nested-PCR protocol in which we found two positive samples of serum, two of saliva and one of urine. This report shows the need for an efficient surveillance system to control the spread of this virus in the population and suggests the use of saliva and urine samples as an alternative for the detection of OROV when serum samples are not available.

著者

Kouji Kida Yasuhiro Matsuoka Tetsuya Shimoda Hiroaki Matsuoka Haruto Yamada Takashi Saito Osamu Imataki Norimitsu Kadowaki Keita Noguchi Ken Maeda Yasushi Mochizuki Toshio Kishimoto

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.72, no.5, pp.356-358, 2019 (Released:2019-09-19)

参考文献数

10

被引用文献数

70

著者

Kazuya Shirato Naganori Nao Shutoku Matsuyama Tsutomu Kageyama

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2019.400, (Released:2019-12-25)

参考文献数

21

被引用文献数

26

---

Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) is usually diagnosed through highly sensitive and specific genetic tests such as real-time reverse transcription polymerase chain reaction (RT-PCR). Currently, two real-time RT-PCR assays targeting the upE and ORF1a regions of the MERS-CoV genome are widely used and are the standard assays recommended by the World Health Organization (WHO). The MERS outbreaks to date suggest that rapid diagnosis and subsequent isolation of infected patients, particularly superspreaders, are critical for containment. However, conventional real-time RT-PCR assays require large laboratory instruments, and amplification takes approximately 2 h. These are disadvantages for rapid diagnosis. Here, an ultra-rapid real-time RT-PCR test was established: a multiplex assay for upE and ORF1a running on the mobile PCR1100 device. As few as five copies of MERS-CoV RNA can be detected within 20 min using the WHO standard assays with similar sensitivity and specificity to those of a conventional real-time PCR instrument such as the LightCyler, enabling timely intervention to control MERS-CoV infection.

著者

Md Shafiullah Parvej Md Ashraful Alam Mio Shono Mst Nusrat Zahan Mst Misrat Masuma Parvez Wahedul Karim Ansari Md Sayduzzaman Jowel Md Sharif Uddin Eriko Kage-Nakadai Md Tanvir Rahman Yoshikazu Nishikawa

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.73, no.1, pp.76-82, 2020 (Released:2020-01-23)

参考文献数

35

被引用文献数

4

---

Using multiplex real-time PCR, 960 fecal samples collected from poultry, cattle, and patients with diarrhea in Bangladesh were screened for diarrheagenic Escherichia coli (DEC). The invasion-related gene virB showed the highest prevalence in human patients (41%) and was shown to be positively correlated first with afaB with regards to diffuse adhesion and second with aggR with regards to aggregative adhesion. These three genes were specific to human patients. In contrast, the Shiga toxin genes stx1 (57%) and stx2 (40%) were prevalent in cattle samples. The eae gene, which is associated with attaching and effacing lesion formation, and the elt and est genes, which are associated with enterotoxins, were detected from all three sample sources. Heat map construction and hierarchical clustering assigned the samples into five different clusters, with the patient samples positive for virB and afaB being placed together in one cluster. Although the detection of virulence genes cannot be a direct indication of the distribution of diarrheagenic organisms, their detection suggests that Shigella spp. or enteroinvasive E. coli are the most prevalent diarrheagenic bacteria in Bangladesh and that diffusely adherent E. coli is concomitantly present with these bacteria. eae-possessing organisms in patients may come from cattle and poultry sources. The small number of stx-positive patients could be explained by the small number of animal samples that were positive for both eae and stx.

著者

Qing He Chuanfei Yu Lan Wang Yongbo Ni Heng Zhang Ying Du Hua Gao Junzhi Wang

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2019.163, (Released:2019-10-31)

参考文献数

27

被引用文献数

3

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Fever is a systemic inflammatory response of the body to pyrogens. Nuclear factor κB (NF-κB) is a central signalling molecule that causes the excessive secretion of various proinflammatory factors induced by pyrogens. This study explored the feasibility of a novel reporter gene assay (RGA) for pyrogen detection using RAW264.7 cells stably transfected with the NF-κB reporter gene as a pyrogenic marker. The RGA could detect different types of pyrogens, including the lipopolysaccharide of gram-negative bacteria, the lipoteichoic acid of gram-positive bacteria, and the zymosan of fungi, and a good dose-effect relationship was observed in terms of NF-κB activity. The limits of detection of the RGA to those pyrogens were 0.03 EU/ml, 0.001 μg/ml, and 1μg/ml, respectively. The method had good precision and accuracy and could be applied to many products [e.g., nivolumab, rituximab, bevacizumab, etanercept, basiliximab, Haemophilus influenza type b conjugate vaccine, 23-valent pneumococcal polysaccharide vaccine, group A and group C meningococcal conjugate vaccine, diphtheria, tetanus, pertussis (acellular, component), poliomyelitis (inactivated) vaccine, and imject alum adjuvant]. The results of this study suggest that the novel RGA has a wide pyrogen detection spectrum and is sufficiently sensitive, stable, and accurate for various applications.

著者

Hiroshi Yoshikura

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2015.420, (Released:2016-01-08)

参考文献数

10

被引用文献数

2 5

著者

Fumihiko Takeuchi Tsuyoshi Sekizuka Akifumi Yamashita Yumiko Ogasawara Katsumi Mizuta Makoto Kuroda

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.67, no.1, pp.62-65, 2014 (Released:2014-01-22)

参考文献数

13

被引用文献数

21 38

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Next-generation DNA sequencing technologies have led to a new method of identifying the causative agents of infectious diseases. The analysis comprises three steps. First, DNA/RNA is extracted and extensively sequenced from a specimen that includes the pathogen, human tissue and commensal microorganisms. Second, the sequenced reads are matched with a database of known sequences, and the organisms from which the individual reads were derived are inferred. Last, the percentages of the organisms' genomic sequences in the specimen (i.e., the metagenome) are estimated, and the pathogen is identified. The first and last steps have become easy due to the development of benchtop sequencers and metagenomic software. To facilitate the middle step, which requires computational resources and skill, we developed a cloud-computing pipeline, MePIC: “Metagenomic Pathogen Identification for Clinical specimens.” In the pipeline, unnecessary bases are trimmed off the reads, and human reads are removed. For the remaining reads, similar sequences are searched in the database of known nucleotide sequences. The search is drastically sped up by using a cloud-computing system. The webpage interface can be used easily by clinicians and epidemiologists. We believe that the use of the MePIC pipeline will promote metagenomic pathogen identification and improve the understanding of infectious diseases.

著者

Shinya Murata Yuzuru Takeuchi Kou Yamanaka Jun Hayakawa Masashige Yoshida Ruriko Yokokawa Akira Wakana Miyuki Sawata Yoshiyuki Tanaka

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2018.448, (Released:2019-05-31)

参考文献数

30

被引用文献数

3

---

Human papillomavirus (HPV)-associated disease is common among men with HPV infection. A quadrivalent HPV (qHPV) vaccine has demonstrated 85.9% efficacy against HPV6/11/16/18-related persistent (≥6 month) infection in a study of Japanese men aged 16–26 yr. Here, we report results of an open-label study of the immunogenicity and tolerability of the qHPV vaccine (NCT02576054), conducted to bridge findings in Japanese men to Japanese boys aged 9–15 yr. A total of 100 boys completed a three-vaccination regimen (Day 1, Months 2, and 6), and 99 boys were included in the primary analysis population. The rate of seroconversion at 1 month after vaccine Dose 3 (Month 7) was high for each vaccine HPV type (anti-HPV6/11/16/18 seroconversion rates [95% CI]: 94.9% [85.5%, 98.3%], 99.0% [94.4%, 100.0%], 99.0% [94.5%, 100.0%], and 99.0% [94.4%, 100.0%], respectively) and anti-HPV6/11/16/18 geometric mean titers were 482.9 mMU/mL, 1052.8 mMU/mL, 3878.3 mMU/mL, and 1114.5 mMU/mL, respectively. Immune responses to qHPV vaccine were non-inferior among Japanese boys included in the current study compared with young Japanese men in a separate study. Injection-site reactions were the most common adverse events, and administration of the vaccine was well tolerated in Japanese boys.

著者

Hiroshi Yoshikura

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.72, no.6, pp.359-367, 2019 (Released:2019-11-21)

参考文献数

17

被引用文献数

1 2

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From 1995 to 2000, the mortality rates of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) patients < 54 years of age have declined; however, since 2000, deaths of HIV/AIDS patients aged > 55 have started to increase. Although deaths directly linked to infections have declined since 2005, those related to malignancy, encephalopathy, interstitial pneumonia, wasting syndrome, etc. persisted. In 10 years from 1999–2004 to 2010–2017, the age at death shifted by 5 years towards an older age in the general population and in patients with HIV/AIDS (mainly males), adult T-cell leukemia, or Creutzfeldt-Jakob disease. Among these, HIV/AIDS patients and the general population exhibited an unequivocal gender difference. As of 2011–2016, the median of the deaths of the HIV/AIDS patients was 52.5 years for males and 70 years for females, while the median of the deaths of the general population was 75 years for males and 85 years for females. Hence, male HIV/AIDS patients died 22.5 years earlier and female HIV/AIDS patients 15 years earlier than did the general population. A common denominator of HIV/AIDS-related deaths and deaths among the general population could be CD4+T cells as these cells are primary targets of HIV, and a decline in naïve CD4+T cell count is a hallmark of aging.

著者

Hiroshi Yoshikura

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.72, no.1, pp.23-30, 2019-01-31 (Released:2019-01-23)

参考文献数

16

被引用文献数

3 7

---

In Japan, HIV infection is classified as “HIV” or “AIDS” depending upon whether the infection was detected before or after the development of AIDS. In male homosexuals, in the plot of the number of “AIDS” notified annually versus the that of “HIV” notified annually, the plot fell on a straight line with a slope close to 1. When the number of “HIV” no longer increased, that of “AIDS” also stopped increasing. The number of “HIV” notified in one region or age group was correlated with that of other regions or age groups, respectively. However, no such correlation was observed among male heterosexuals. “HIV” was detected more frequently among male homosexuals and females than among male heterosexuals. The rate of “AIDS” detection increased with age in all infection categories. Our analysis, supported by findings of other studies, suggested that the higher rate of “HIV” detection among male homosexuals and females was attributed to the increased risk of receptive sexual intercourse, while the higher rate of “AIDS” detection among the elderly people was attributed to immunological senescence.

著者

岩佐 三郎 石田 説而 赤真 清人

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Medical Science and Biology (ISSN:00215112)

巻号頁・発行日

vol.38, no.2, pp.53-65, 1985 (Released:2010-03-19)

参考文献数

35

被引用文献数

3 3

---

Intracerebral injection of vaccine into the mouse induced swelling of the brain. The swelling reached the maximum in the intensity by day 1 and persisted for several days. A method for quantitative determination of the brain-swelling activity of the vaccine was developed. A positive regression coefficient was found only between the brain-swelling and the lymphocytosis-promoting activities. Such activity was no longer shown with the vaccine heat-treated for 30 min at 80 C, but it was restored upon addition of the lymphocytosis-promoting factor (LPF) that caused no brain swelling by itself. The activity, therefore, was ascribed to cooperation of LPF and a certain heat-stable component other than endotoxin contained by pertussis vaccine.

著者

Hiroshi Yoshikura

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2018.421, (Released:2019-04-26)

参考文献数

7

被引用文献数

1

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The number of eaters and that of patients per outbreak followed lognormal distribution in food poisonings caused by microbes while it followed scale-free distribution in food poisoning caused by plant or animal toxins. Attack rates of the individual outbreaks were distributed continuously and almost linearly from >0 to 1 for all the food poisonings, i.e., they could not be represented by median and standard deviation. For monitoring of the number of patients and the attack rate in individual outbreaks simultaneously, the number of patients was plotted in the x-axis in the logarithmic scale against the attack rate in the y-axis in the normal scale. There emerged plots characterized by repeating arcs assuming shape of a butterfly with extended wings viewed from above, which was called “backbone configuration”. The butterfly-shaped plot patterns were generally stable over time, but variable depending on pathogens, implicated facilities and their combinations. The backbone configuration was reproduced by assuming that the number of patients per outbreak was distributed continuously from 1 to the number of eaters per outbreak.

著者

Yuichi Fukui Hisashi Inokuma

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2018.470, (Released:2019-01-31)

参考文献数

18

被引用文献数

10

---

The prevalence of Anaplasma infection in 332 dogs from Ibaraki, Japan, was evaluated by serological and molecular surveys. Immunofluorescence antibody assay (IFA) against Anaplasma phagocytophilum indicated that 7 (2.1%) of 328 dogs were positive for A. phagocytophilum. Screening PCR demonstrated that 8 (2.4%) of 331 dogs were positive for Anaplasmataceae. Phylogenetic analysis of the partial 16S rRNA sequence of PCR amplicons revealed that 6 sequences were most similar to the 16S rRNA sequence of Wolbachia sp. and the remaining 2 to Anaplasma bovis. Further analysis by A. phagocytophilum-specific nested PCR demonstrated that 1 dog infected with A. bovis was also positive for A. phagocytophilum. This is the first study to report the dual infection of a dog in Japan with A. bovis and A. phagocytophilum.

著者

Tetsuo Nakayama Shigeru Suga Kenji Okada Nobuhiko Okabe

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.72, no.1, pp.49-52, 2019-01-31 (Released:2019-01-23)

参考文献数

23

被引用文献数

7

---

A follow-up serological study was conducted involving 47 subjects who received 4 doses of diphtheria and tetanus toxoids, combined with the acellular pertussis vaccine (DTaP) together with Salk-type inactivated polio vaccine (DTaP-wIPV), until 6 years of age. All antibody levels declined more rapidly than expected within 3 years after the completion of primary vaccination with the 4th booster dose, and titers persisted until 6 years of age. The positive rate of the IgG antibody against pertussis toxin (PT) was 31.9% (15/47) at 4 years of age, 41.0% (16/39) at 5 years of age, and 40.5% (15/37) at 6 years of age. A significant increase in anti-PT antibodies was observed in 6 subjects, suggesting subclinical infection. Positive rates of antibodies against other targets did not decrease; however, titers of neutralizing antibodies against poliovirus type III decreased in a few subjects. These data suggest the need for an additional preschool booster immunization using DTaP-wIPV.

著者

Akio Yamada Kohei Makita Hazumu Kadowaki Naoto Ito Makoto Sugiyama Nigel C.L. Kwan Katsuaki Sugiura

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2018.431, (Released:2018-12-25)

参考文献数

58

被引用文献数

10

---

Although rabies still kills many people, global eradication of human rabies is considered to be feasible. Because the progress toward eradication may be different among regions with different socio-economic status, states that successfully eradicate this disease must be vigilant for re-emergence of rabies. In this article, we describe arguments over current rabies prevention measures and risk assessment results for rabies introduction and spread in rabies-free Japan. We also summarize the measures taken by representative countries and regions free from rabies. Our risk assessment results revealed that the risk of rabies reintroduction under current circumstances is very low, and that subsequent spread of the disease would be minimal because of quite low value of basic reproduction number. Similar assessments conducted in other rabies-free areas also showed quite limited risks of introduction. The majority of rabies-free countries maintain their rabies-free status through strict import quarantine of carnivorous animals, efficient surveillance of animal rabies including wildlife, quick emergency responses, and raising public awareness of the disease. To keep current rabies-free status in Japan, it is strongly recommended to maintain current quarantine system as well as to reinforce compliance of stakeholders involved in international dog movement. Sustainable surveillance system targeting wildlife is also indispensable.

著者

Tetsuo Nakayama Shigeru Suga Kenji Okada Nobuhiko Okabe

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2018.314, (Released:2018-10-31)

参考文献数

23

被引用文献数

7

---

A follow-up serological study was conducted involving 47 subjects who received four doses of diphtheria and tetanus toxoids combined with acellular pertussis vaccine (DTaP) together with Salk-type inactivated polio vaccine (DTaP-wIPV) until 6 years of age. All antibody levels declined more rapidly than expected within 3 years after the completion of the primary vaccination with 4th booster dose and titers persisted until 6 years of age. The positive rate of the IgG antibody against pertussis toxin (PT) was 31.9% (15/47) at 4 years of age, 41.0% (16/39) at 5 years of age, and 40.5% (15/37) at 6 years of age. A significant increase in the PT antibody was observed in 6 subjects, suggesting subclinical infection. Positive rates of antibodies against other targets did not decrease but titers of neutralizing antibodies against poliovirus type III decreased in only a few subjects. These data suggest the need for an additional preschool booster immunization using DTaP-wIPV.

著者

Yuichi Fukui Seigo Ohkawa Hisashi Inokuma

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.71, no.4, pp.302-305, 2018-07-31 (Released:2018-07-24)

参考文献数

15

被引用文献数

7

---

Anaplasma phagocytophilum DNA was detected from a dog with canine granulocytic anaplasmosis (CGA) in Japan. Phylogenetic analysis of the DNA using 16S rRNA, gltA, and groEL sequences revealed that the strain was nearly identical to A. phagocytophilum detected from Apodemus agrarius (black-striped field mouse) in China and Korea. To our knowledge, this is the first report of molecular detection and phylogenetic analysis of A. phagocytophilum from a clinical case of CGA in Japan.

著者

Hiroshi Yoshikura

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2018.183, (Released:2018-09-28)

参考文献数

16

被引用文献数

7

---

In Japan, HIV infection is classified as “HIV” or “AIDS” depending upon whether the infection was detected before or after development of AIDS. For male homosexuals, in the plot of the number of “AIDS” versus the number of “HIV”, the plots fell on a straight line with a slope close to 1. When the number of “HIV” stopped increasing, that of “AIDS” stopped increasing. The number of “HIV” in one region or in one age group was correlated with that of other regions or that of other age groups, respectively. No such correlation was detected for male heterosexuals. Male homosexuals and females were detected more as “HIV” than male heterosexuals. Detection as “AIDS” increased with age in all the infection categories. Our analysis supported by others suggested that the higher rate of detection as “HIV” among male homosexuals and females than among male heterosexuals was attributable to higher risk of the receptive sexual intercourse, and the higher incidence of “AIDS” among elderlies to immunological senescence.

著者

Xiaochen Liu Wenxiao Jia Hong Wang Yunling Wang Jingxun Ma Hao Wang Xuan Zhou Guohua Li

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.68, no.2, pp.89-97, 2015 (Released:2015-03-23)

参考文献数

34

被引用文献数

4 8

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This study aimed to establish a spinal tuberculosis model by implanting Mycobacterium tuberculosis strain H37Rv into the lumbar vertebral body of New Zealand white rabbits. A hole was first drilled into the top of the 6th lumbar vertebra of each rabbit, which was then filled with a gelatin sponge to adsorb 0.2 ml of M. tuberculosis suspension (107 CFU /ml) for the infection group or normal saline for the control group. The holes were then closed with sutures. CT findings demonstrated that 5 and 10 rabbits developed spinal tuberculosis at 4 and 8 weeks, respectively, after this procedure. MRI examinations revealed that 7 and 15 rabbits had positive results at 4 and 8 weeks, respectively, after this procedure. HE staining of the vertebral body and paravertebral soft tissue biopsies of infected rabbits indicated inflammatory cell infiltration or necrosis in 15 rabbits. M. tuberculosis was cultured in 67% of the abscesses. The modeling success rate was 68.1%. By implanting an appropriate dosage of M. tuberculosis strain H37Rv into a local lumbar vertebral body of New Zealand white rabbits, we successfully established a spinal tuberculosis model, the pathological changes of which are similar to those of human spinal tuberculosis.