著者

高瀬 公三 馬場 守 ジルベルト 有吉 理佳子 藤川 英雄

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.58, no.11, pp.1129-1131, 1996

被引用文献数

7

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SPF発育鶏胚の高度病原性伝染性ファブリキウス嚢病ウイルス(hv IBDV)F539株及びDV86株に対する感受性を, 3つの接種ルートで, 従来型IBDV G691株と比較しながら調べた. その結果, 鶏胚のhv IBDV 2株に対する感受性はG691株より高く, 感染胚のほぼ100%が死亡し, 算出された50%胚致死量は50%胚感染量とほぼ同値を示した. 鶏胚に対する高い致死性はhv IBDVに特徴的な性状と思われた.

著者

高瀬 公三 馬場 守ジルベルト 西 理佳子 藤川 英雄 山田 進二

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.57, no.2, pp.327-330, 1995-04-15

被引用文献数

2

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発育鶏卵漿尿膜(CAM)及び鶏腎培養細胞(CK)由来の鶏アデノウイルス(FAV)寒天ゲル内沈降(AGP)抗原の反応性を実験感染鶏血清及び野外鶏血清を用いて比較し, 次の成績を得た. 実験感染鶏血清の両由来抗原による陽性率はFAVの株(血清型)の違いによって異なり, ヘテロ抗原での陽性率はホモ抗原でのそれに比べて低く, またヘテロ抗原での抗体価もホモ抗原の抗体価に比べて低い傾向を示した. さらに, CAM由来抗原を用いた方がCK由来抗原の場合よりも高い陽性率, 又は高い抗体価の得られる場合が多かった. CAM由来抗原を用いて野外鶏血清を検査したところ, 用いる抗原(血清型)によって抗体陽性率は異なったが, 複数の抗原を混合することにより, 陽性率は高まった. このことから, FAVの血清疫学調査にはCAM由来の複数の血清型のAGP抗原を用いて行うことが望ましいと考えられた.

著者

Genta ITO Kuniko YOSHIMURA Yasuyuki MOMOI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0341, (Released:2017-03-04)

被引用文献数

10

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Reliable methodology for predicting the age of mature dogs is currently unavailable. In this study, amplicon sequencing of 50 blood samples obtained from diseased dogs was used to measure methylation in seven DNA regions. Significant correlations between methylation level and age were identified in four of the seven regions. These four regions were then tested in samples from 31 healthy toy poodles, and correlations were detected in two regions. The age of another 11 dogs was predicted using data from the diseased dogs and the healthy poodles. The mean difference between the actual and calculated ages was 34.3 and 23.1 months, respectively. Further research is needed to identify additional sites of age-related methylation and allow accurate age prediction in dogs.

著者

Angeline Ping Ping TEH Watanyoo PRATAKPIRIYA Yuichi HIDAKA Hiroyuki SATO Takuya HIRAI Ryoji YAMAGUCHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0501, (Released:2017-02-27)

被引用文献数

4

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A 3.5-year-old female Chihuahua was presented with complaint of neck pain, intermittent cough and dysphagia. Physical examination and diagnostic imaging of neck region revealed a solid and highly vascularized mass involving the retropharyngeal region. Histologically, the mass showed an atypical zellballen pattern which comprised of high density of type I chief cells with high nuclear cytoplasmic ratio and separated by delicate fibrovascular stroma. Immunoreactivity for neuroendocrine markers was diffusely positive in cytoplasm of tumor cells. Disseminated tumor emboli in external jugular vein were detected 6 months after initial surgery. An electron microscopic study revealed numerous electron-dense intracytoplasmic neurosecretory granules. Based on these findings, carotid body carcinoma was diagnosed.

著者

アハメド モハメド 木村 和弘 ソリマン モハメド 山地 大介 岡松(小倉) 優子 マコンド ケネディ 稲波 修 斉藤 昌之

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.69, no.2, pp.125-131, 2007-02-25

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脂肪細胞から分泌されるレプチンは摂食調節やエネルギー消費を調節するだけでなく,直接あるいは腫瘍壊死因子(TNF-α)を介して間接的にヒト好中球機能を修飾する.ウシ好中球はfMLP受容体を持たないなどヒト好中球とは異なる特徴を持つ.そこで本研究ではホルスタイン牛好中球の脱顆粒応答および活性酸素産生能に村するレプチンとTNF-αの作用を調べた.なおウシ好中球には活性型レプチン受容体Ob-RbとTNF-α受容体(TNF-R1)の発現が確認された.ヒトレプチン,ヒトTNF-α,活性型ホルボールエステル(PMA)とオプソニン化したザイモザン(OZP)は脱顆粒を惹起しなかった.一方,アナフィラトキシンを含むザイモザン活性化血清は(ZAS)は脱顆粒を誘導した.しかし,レプチンやTNF-αはZASによる脱顆粒反応にも影響しなかった.レプチンを除く,TNF-α, PMA, OZPとZASは活性酸素の産生を異なる強度で誘導した.さらにTNF-αはOZPとZASによる活性酸素産生能を増強し,この活性増大作用の一部分はNADPHオキシダーゼの細胞質コンポーネントであるp47^<phox>の膜移行の増加で説明された.レプチンはいずれの刺激に対しても影響を示さなかった.これらの結果よりヒト好中球とは異なりレプチンはウシ好中球の脱顆粒応答および活性酸素産生能に村して直接的な作用を示さないことが明らかとなった.

著者

アハメド モハメド シャバン ゼイン 山地 大介 岡松(小倉) 優子 ソリマン モハメド アブエルダイム マブルク 石岡 克己 マコンド ケネディ 斉藤 昌之 木村 和弘

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.69, no.5, pp.509-514, 2007-05-25

被引用文献数

11

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脂肪細胞分泌因子,レプチンは摂食やエネルギー消費のみならず,免疫細胞機能を修飾することが知られる.ウシ単核球(PBMC)をレプチン単独あるいはT細胞マイトージェンCon Aと同時に刺激すると,レプチンはPBMCの増殖を10-40%増大させた.一方,PBMCからTリンパ球を分離し,同様に刺激すると,レプチンはTリンパ球の増殖を抑制したので,レプチンはPBMCに含まれる単球マクロファージなどに作用してTリンパ球増殖因子を産生させる可能性を示した.次に単球マクロファージを分離してサイトカイン遺伝子の発現を調べたところ,多寡はあるものの腫瘍壊死因子TNF-α,インターロイキン(IL)-1β,IL-12p35,IL-12p40,IL-18遺伝子の恒常的な発現が見られた.レプチンを作用させるとTNF-αとIL-12p40mRNAの発現が増大したが,他の遺伝子の発現は変化しなかった.TNF-αの分泌量を調べると,実際に培養液中の濃度が増加した.また培養液中のIL-1β濃度が増大した.そこで伏在性のpro-IL-1βやpro-IL-18を活性型に分解させるタンパク分解酵素caspase-1の発現について調べたところ,レプチンによってその遺伝子の発現が増大した.これらの結果より,レプチンは単球マクロファージに作用しIL-12p35/p40の複合体形成や活性型IL-1β/IL-18を分泌させTリンパ球の増殖を促進すると考えられた.

著者

Srinuan SOMROOP Noritoshi HATANAKA Sharda Prasad AWASTHI Kentaro OKUNO Masahiro ASAKURA Atsushi HINENOYA Shinji YAMASAKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0654, (Released:2017-02-16)

被引用文献数

3

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Cytolethal distending toxin (CDT) consisting of CdtA, CdtB and CdtC has been reported to be a possible virulence factor of campylobacters including C. upsaliensis. In our previous study, the cdtB gene-based PCR-restriction fragment length polymorphisms (RFLP) assay for detection and differentiation of 7 Campylobacter species yielded 3 different RFLP patterns (Cu-I to Cu-III). In this study, entire cdt (Cucdt) genes of each pattern were sequenced to see whether there are any differences in cdt genes, its amino acid sequences and biological activity of CuCDT. We found that all 3 representative strains harbor the entire Cupcdt genes and homology between prototype and newly determined Cucdt genes was 94 to 98% with cdtA, 93 to 94% with cdtB and 92 to 93% with cdtC, while that between amino acids of CuCDT was 95 to 99% with CdtA, 97 to 98% with CdtB and 92 to 93% with CdtC. Furthermore, CDT activity produced by C. upsaliensis strains was examined by cytotoxicity assay with HeLa cells. Interestingly, C. upsaliensis produced 64 to 2,340 times higher CDT titer in comparison to other campylobacters did. In addition, Cu-III showed 64 times higher CDT titer than Cu-II, although CDT production level was almost the same by western blotting. These data suggest that CDT produced by C. upsaliensis might contribute more to human diseases in comparison to that produced by other campylobacters and Cu-III CDT seems to be more toxic to HeLa cells in comparison to Cu-I and Cu-II CDTs.

著者

Yeon Soo CHUNG Young Kyung PARK Yong Ho PARK Kun Taek PARK

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0585, (Released:2017-02-11)

被引用文献数

6

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Companion animals are considered as one of the reservoirs of antimicrobial-resistant (AR) bacteria that can be cross-transmitted to humans. However, limited information is available on the possibility of AR bacteria originating from companion animals being transmitted secondarily from owners to non-owners sharing the same space. To address this issue, the present study investigated clonal relatedness among AR E. coli isolated from dog owners and non-owners in the same college classroom or household. Anal samples (n=48) were obtained from 14 owners and 34 non-owners; 31 E. coli isolates were collected (nine from owners and 22 from non-owners). Of 31 E. coli, 20 isolates (64.5%) were resistant to at least one antimicrobial, and 16 isolates (51.6%) were determined as multi-drug resistant E. coli. Six isolates (19.4%) harbored integrase genes (five harbored class I integrase gene and one harbored class 2 integrase gene, respectively). Pulsed-field gel electrophoretic analysis identified three different E. coli clonal sets among isolates, indicating that cross-transmission of AR E. coli can easily occur between owners and non-owners. The findings emphasize a potential risk of spread of AR bacteria originating from pets within human communities, once they are transferred to humans. Further studies are needed to evaluate the exact risk and identify the risk factors of secondarily transmission by investigating larger numbers of isolates from pets, their owners and non-owners in a community.

著者

Ken-ichi WATANABE James K. CHAMBERS Kazuyuki UCHIDA Kazumi NIBE Nanako USHIO Noriyuki HORIUCHI Yoshiyasu KOBAYASHI Hiroyuki NAKAYAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0610, (Released:2017-01-28)

被引用文献数

5

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The atypical cutaneous tumor of a 9-year-old mixed breed female dog was examined. The tumor was well-demarcated and histologically composed of a trichoblastic area, tricholemmal area and apocrine glandular area. Neoplastic cells in trichoblastic area and tricholemmal area had PAS-positive granules in the cytoplasm and were positive for pan-cytokeratin, cytokeratin 5/6, 14 and 19 and p63. Neoplastic cells in trichoblastic area were also positive for cytokeratin 15 and CD34. Neoplastic cells in apocrine glandular area were positive for pan-cytokeratin and cytokeratin 7, 18 and 19. Myoepithelial cell proliferation with osteocartilaginous metaplasia was observed in this area. Since neoplastic cells showed multiphenotypic differentiation for hair follicles and apocrine glands, the present case was diagnosed as a cutaneous mixed tumor.

著者

Sungjun WON Sookyung YUN Jeosoon LEE Mikyung LEE Mincheol CHOI Junghee YOON

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0476, (Released:2017-02-05)

被引用文献数

5

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The objective of study is to determine the thickness of bronchial walls of clinically diagnosed asthmatic cats using high resolution computed tomography (HRCT) compared to that of healthy cats. The bronchial walls and pulmonary arteries were measured in healthy 16 cats and clinically asthmatic 4 cats. The bronchial walls and pulmonary arteries were measured under general anesthesia with positive pressure inspiration using HRCT. In healthy and asthmatic cats, bronchial lumen to the artery ratio (BA ratio), the ratio of bronchial wall thickness to bronchial diameter (TD ratio) and ratio of bronchial wall thickness to pulmonary artery (TA ratio) were measured. The mean BA ratio, TD ratio and TA ratio in healthy cats were 0.86 ± 0.12, 0.18 ± 0.02 and 0.25 ± 0.05, respectively. Under the same condition, the mean BA ratio, TD ratio and TA ratio in asthmatic cats were 0.93 ± 0.21, 0.22 ± 0.24 and 0.37 ± 0.06. The TD ratio and TA ratio in asthmatic cats were significantly higher than healthy cats (P<0.001). BA ratio was not significantly different in both groups (P>0.05). The evaluation of bronchial wall thickness by HRCT could be useful for diagnosis of disease of bronchial wall thickening, such as feline asthma.

著者

Manabu KOIKE Yasutomo YUTOKU Aki KOIKE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0649, (Released:2017-02-06)

被引用文献数

11

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Understanding the molecular mechanisms of DNA double-strand break (DSB) repair machinery, specifically non-homologous DNA-end joining (NHEJ), is crucial for developing next-generation radiotherapies and common chemotherapeutics for human and animal cancers. The localization, protein-protein interactions and post-translational modifications of core NHEJ factors, might play vital roles for regulation of NHEJ activity. The human Ku heterodimer (Ku70/Ku80) is a core NHEJ factor in the NHEJ pathway and is involved in sensing of DSBs. Companion animals, such as canines, have been proposed to be an excellent model for cancer research, including development of chemotherapeutics. However, the post-translational modifications, localization and complex formation of canine Ku70 have not been clarified. Here, we show that canine Ku70 localizes in the nuclei of interphase cells and that it is recruited quickly at laser-microirradiated DSB sites. Structurally, two DNA-PK phosphorylation sites (S6 and S51), an ubiquitination site (K114), two canonical sumoylation consensus motifs, a CDK phosphorylation motif, and a nuclear localization signal (NLS) in the human Ku70 are evolutionarily conserved in canine and mouse species, while the acetylation sites in human Ku70 are partially conserved. Intriguingly, the primary candidate nucleophile (K31) required for 5’dRP/AP lyase activity of human and mouse Ku70 is not conserved in canines, suggesting that canine Ku does not possess this activity. Our findings provide insights into the molecular mechanisms of Ku-dependent NHEJ in a canine model and form a platform for the development of next-generation common chemotherapeutics for human and animal cancers.

著者

宇根 有美 多々良 成紀 野村 靖夫 高橋 令治 斎藤 保二

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.58, no.9, pp.933-935, 1996-09-25

被引用文献数

1

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2頭のオグロプレーリードッグ(Cynomys ludovicianus)に, 肺転移を伴う肝細胞癌と非腫瘍部の慢性活動性肝炎の同時発生と, 肝細胞の過形成が認められた. 過形成性の肝細胞は細胞質内に多数の好酸性, Orcein染色陽性の封入体を持っていた. 電子顕微鏡で, 細胞質内封入体に一致して鎖かたびらのような編み目状の構造物を観察した. これらの封入体の電顕像は, 肝癌に関連するヘパドナウイルスの封入体の形態と異なっていた. また, ヘパドナウイルス様の粒子も認められなかった.

著者

Nozomu YOKOYAMA Hiroshi OHTA Yumiko KAGAWA Rommaneeya LEELA-ARPORN Angkhana DERMLIM Khoirun NISA Tomoya MORITA Tatsuyuki OSUGA Noboru SASAKI Keitaro MORISHITA Kensuke NAKAMURA Mitsuyoshi TAKIGUCHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0479, (Released:2017-01-16)

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We examine the expression of tight junction and adherence junction proteins in the colorectal mucosa of miniature dachshunds (MDs) with inflammatory colorectal polyps (ICRPs). Colorectal mucosa samples were endoscopically obtained from 8 MDs with ICRPs and 8 control dogs for immunoblotting. Paraffin-embedded tissues of surgically resected inflamed lesions from another 5 MDs with ICRPs and full-thickness colorectal specimens from 5 healthy beagles were obtained for immunohistochemistry. The expression patterns of claudin-1, -2, -3, -4, -5, -7 and -8, E-cadherin and β-catenin were analyzed in the non-inflamed mucosa and inflamed mucosa of ICRPs and colorectal mucosa of control dogs by immunoblotting. The localization of these proteins in the inflamed lesions was analyzed by immunohistochemistry. The expressions of each of claudin, E-cadherin and β-catenin were not significantly different between control dogs and non-inflamed colonic mucosa from MDs with ICRPs. In contrast, only E-cadherin and β-catenin were detected in the inflamed lesions of MDs with ICRPs. By immunohistochemistry, claudin-2, -3, -4, -5 and -7, E-cadherin and β-catenin were expressed in the colorectal epithelium within the inflamed mucosa, but not in granulation tissue. Distributions of claudin-2, -3, -4, -5 and -7, E-cadherin and β-catenin in the colonic epithelium were not different between MDs with ICRPs and control dogs. These results indicated that no significant alteration was detected in several tight junction or adherence junction proteins expression in the colorectal epithelium of ICRPs.

著者

Eunji KOH Yun Chan JUNG Heung-Myong WOO Byung-Jae KANG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0456, (Released:2017-01-06)

被引用文献数

14

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The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were formed by vibrational technology and the production of injectable microbeads was performed using various parameters with standard methodology. Microbead toxicity was tested in an animal model. Encapsulated cASCs were evaluated for viability and proliferation in vitro. HEK-293 cells, with or without microencapsulation, were injected into the subcutaneous tissue of mice and were tracked using in vivo bioluminescent imaging to evaluate the retention of transplanted cells. The optimized injectable microbeads were of uniform size and approximately 250 μm in diameter. There was no strong evidence of in vivo toxicity for the alginate beads. The cells remained viable after encapsulation, and there was evidence of in vitro proliferation within the microcapsules. In vivo bioluminescent imaging showed that alginate encapsulation improved the retention of transplanted cells and the encapsulated cells remained viable in vivo for 7 days. Encapsulation enhances the retention of viable cells in vivo and might represent a potential strategy to increase the therapeutic potency and efficacy of stem cells.

著者

Hironari OSADA Misato OGAWA Ayana HASEGAWA Makoto NAGAI Junsuke SHIRAI Kazuaki SASAKI Minoru SHIMODA Hiroshi ITOH Hirotaka KONDO Keitaro OHMORI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0451, (Released:2016-12-29)

被引用文献数

11

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It remains unclear whether epithelial cell-derived cytokines, including interleukin (IL)-25, IL-33 and thymic stromal lymphopoietin (TSLP), contribute to development of canine chronic enteropathy (CE), which includes antibiotic-responsive enteropathy (ARE), food-responsive enteropathy (FRE) and inflammatory bowel disease (IBD). In the present study, we examined mRNA expression of il-25, il-33 and tslp in the duodenal and colonic mucosae of dogs with ARE, FRE and IBD. Real-time PCR analysis revealed that mRNA expression of il-33 was significantly lower in the duodenum in dogs with FRE than in healthy dogs. The results suggest that epithelial cell-derived cytokines may not be an inducer of Th2-type immunity in the gut of dogs with CE, and decreased expression of IL-33 may be involved in induction of FRE. Further studies are required to clarify roles of epithelial cell-derived cytokines, especially IL-33, in the pathogenesis of canine CE.

著者

Zenta TAKATSU Muneya TSUDA Akio YAMADA Hiroshi MATSUMOTO Akira TAKAI Yasuhiro TAKEDA Mitsunori TAKASE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0450, (Released:2016-12-31)

被引用文献数

8

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Hand-reared elephant calves that are nursed with milk substitutes sometimes suffer bone fractures, probably due to problems associated with nutrition, exercise, sunshine levels and/or genetic factors. As we were expecting the birth of an Asian elephant (Elephas maximus), we analyzed elephant’s breast milk to improve the milk substitutes for elephant calves. Although there were few nutritional differences between conventional substitutes and elephant’s breast milk, we found a large unknown peak in the breast milk during high-performance liquid chromatography-based amino acid analysis and determined that it was glucosamine (GlcN) using liquid chromatography/mass spectrometry. We detected the following GlcN concentrations [mean ± SD] (mg/100 g) in milk hydrolysates produced by treating samples with 6M HCl for 24 hr at 110˚C: four elephant’s breast milk samples: 516 ± 42, three cow’s milk mixtures: 4.0 ± 2.2, three mare’s milk samples: 12 ± 1.2 and two human milk samples: 38. The GlcN content of the elephant’s milk was 128, 43 and 14 times greater than those of the cow’s, mare’s and human milk, respectively. Then, we examined the degradation of GlcN during 0–24 hr hydrolyzation with HCl. We estimated that elephant’s milk contains >880 mg/100 g GlcN, which is similar to the levels of major amino acids in elephant’s milk. We concluded that a novel GlcN-containing milk substitute should be developed for elephant calves. The efficacy of GlcN supplements is disputed, and free GlcN is rare in bodily fluids; thus, the optimal molecular form of GlcN requires a further study.

著者

Yasuhiro TAKASHIMA Isako ONODA Shin-Pin CHIOU Katsuya KITOH

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0461, (Released:2016-12-30)

被引用文献数

2

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Platelet function hyper-activity has been reported in Dirofilaria immitis (heartworm, HW)-infected dogs. Although the mechanism of increased platelet hyper-activity has not yet been elucidated, it is suggested to be mediated by unknown factors, which may be related to adult HW components. This study aims to determine whether adult male HW whole body extract induces canine platelet aggregation in vitro. The results indicate that HW extract caused an aggregation of canine platelets in a concentration-dependent manner. This aggregation ability of the HW extract was not mediated by the adenosine diphosphate receptor. In addition, the mechanisms of aggregation did not require cyclooxygenase-dependent pathways, and the aggregating activity of substances contained in the HW extract was heat stable; therefore, the active substances may be different from collagen. Furthermore, the platelet aggregating activity remained within the molecular weight (MW)≥100,000 fraction obtained by ultrafiltrating the HW extract. In contrast, the MW<100,000 fraction also had a platelet aggregation ability, but the aggregation pattern was reversible and the maximum extent decreased, compared with the MW≥100,000 fraction response. Our experiments have been conducted using a whole body extract from adult HWs to determine with certainty the aggregating activity of HW elements on canine platelets. More studies are necessary to evaluate the effects of the metabolic products released from live adult worms in pulmonary arteries and the symbiont bacterium Wolbachia-derived antigens on canine platelet aggregation.

著者

Yuko KONISHI Hiroyuki SATOH Yasuyoshi KUROIWA Mizuki KUSAKA Atsushi YAMASHITA Yujiro ASADA Taketoshi ASANUMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0504, (Released:2016-12-23)

被引用文献数

9

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Fiber tractography is a technique capable of depicting the three-dimensional structure and connectivity of nerve fibers using serial magnetic resonance diffusion tensor imaging (DTI). To establish fiber tractography and DTI methods in veterinary clinical medicine, we evaluated fiber tractography and DTI parameters: apparent diffusion coefficient (ADC) values and fractional anisotropy (FA) values, in various spinal cord diseases. Spinal cord DTI was examined in 28 dogs with spinal cord diseases. The ADC and FA values were measured at lesion sites and cranial normal sites on spinal cords, and both values of lesion sites were compared with normal sites. In thoracolumbar intervertebral disk herniation (IVDH) cases, depending on their neurologic grades, fiber tractography indicated rupture of fiber trajectories, loss of neuronal bundles and disorder of fiber directions. In these cases, the average ADC values at lesion sites significantly decreased compared with normal sites (P=0.016). In the progressive myelomalacia case, the average ADC and the FA values of hyperintense swollen regions in T2WI decreased compared to both values in other disease cases. Finally, in the meningioma case, the continuity of fiber trajectories improved after the administration of an anticancer agent. This study suggests that fiber tractography and DTI are useful in the diagnosis and prognosis of veterinary spinal cord diseases.

著者

Azusa INUI Kazuhito ITAMOTO Tetsuya TAKUMA Hideki TSUTSUMI Manabu TANIGAWA Mineo HAYASAKI Yasuho TAURA Koichi MAMBA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.66, no.6, pp.599-609, 2004 (Released:2004-07-05)

参考文献数

43

被引用文献数

12 19

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Bone mineral density (BMD), distribution of its density and bone histomorphometric parameters were evaluated in lumbar vertebra of normally growing miniature pigs. The fourth lumbar vertebra (L4) of the Göttingen miniature pig were used in this cross-sectional study in vitro. The BMD of the miniature pig was similar to that of humans in tendency of gender differences and some growth patterns during puberty. In these regards this animal appears useful as a model for human bone study. However, the trabecular and cortical BMDs of lumbar spine were extremely high value (399.43 ± 26.36 mg/cm3 in female trabeculae; 973.06 ± 69.55 mg/cm3 in female cortical bone; 419.04 ± 34.84 mg/cm3 in male trabeculae; 1038.81 ± 125.72 mg/cm3 in male cortical bone in pigs 30 months or more). Furthermore, histomorphometric analysis yielded values that were remarkably different from those found in humans. From these results, it was revealed that miniature pig had a higher bone mass and denser trabecular network than human, indicating that its bone is probably stronger. Therefore, care should be taken in choosing the miniature pig as a bone study model.

著者

Fungwithaya PUNPICHAYA Brikshavana PASAKORN Chanchaithong PATTRARAT Prapasarakul NUVEE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0146, (Released:2016-12-17)

被引用文献数

9

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This study aimed to investigate the spread of methicillin-resistant coagulase-positive staphylococci (MRCoPS) among veterinary staff, hand-touch sites and surgical tissue during cystotomy operations on cats and dogs that were patients, and to analyze the genetic relatedness and antimicrobial resistance profiles of the isolates. Human and environmental samples were obtained from the nasal passageways of 12 surgeons and veterinary assistants and from 29 hand-touch sites of instruments in operative units and subjected to bacterial isolation and enumeration. Swab samples were collected in triplicate from 29 dogs and three cats at the site of incision, from the incision area, from the peritoneum during surgery and from the peritoneum before suture. MRCoPS were identified by mecA gene detection and characterized by their antibiogram profile, SCCmec type and pulsed-field gel electrophoresis. Twenty-four staphylococci were isolated, derived from one veterinary assistant, 12 operating room floor areas and hand-touch sites, three dogs and one cat. Methicillin-resistant S. pseudintermedius (MRSP) was found on an electric clipper and rebreathing circuits in the operating room. Three dogs were positive for MRSP during surgery, and one methicillin-resistant S. aureus (MRSA) was detected in a cat. All MRCoPS were resistant to doxycycline, erythromycin, clindamycin and enrofloxacin, but no patients developed surgical site infections. According to their genotypic patterns, the clones obtained from the environment and human sources differed from the animal clones. Despite intensive hygienic management, a variety of MRCoPS clones were present within the surgical unit and during surgery.