著者

佐塚 泰之 山下 恵代 岸本 修一 福島 昭二 竹内 由和 園部 尚

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.122, no.11, pp.995-999, 2002-11-01 (Released:2003-02-18)

参考文献数

14

被引用文献数

6 8

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We have confirmed that theanine, a major amino acid in green tea, enhances the antitumor activity of doxorubicin (DOX) without an increase in DOX-induced side effects. We believe that the action of theanine is due to decreases in glutamate uptake via inhibition of the glutamate transporter, intracellular glutathione (GSH) synthesis, GS-DOX conjugate level, and subsequent extracellular transport of GS-DOX by the MRP5/GS-X pump. To increase the clinical usefulness of theanine, we examined its effects on the antitumor activity of cisplatin and irinotecan (CPT-11), which a known to be transported by the efflux system related to MRP. Cisplatin decreased tumor volume in M5076 tumor-bearing mice. Furthermore, the combination of theanine with cisplatin increased the decrease in tumor volume as compared with the cisplatin-alone group. Tumor volume in the CPT-11-alone group did not show a decrease, but the combination of theanine with CPT-11 significantly reduced tumor volume. The concentration of cisplatin in the tumor was significantly increased by combination with theanine, and thus we assume that it correlated with the enhancement on the antitumor activity of theanine. On the other hand, changes in drug concentrations with theanine were not observed in normal tissues, but rather it is indicated that theanine tends to reduce their concentrations. Therefore theanine enhances the antitumor activity not only of DOX but also of cisplatin or CPT-11.

著者

松尾 泰佑 富田 隆 工藤 賢三 佐塚 泰之

出版者

一般社団法人日本医療薬学会

雑誌

医療薬学 (ISSN:1346342X)

巻号頁・発行日

vol.45, no.1, pp.54-60, 2019-01-10 (Released:2020-01-10)

参考文献数

9

被引用文献数

1

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Magnesium oxide tablets are sometimes crushed prior to administration to patients having difficulties in swallowing tablets. However, the quality of magnesium oxide formulations may decrease when stored for a long time as a one-dose package, owing to the absorption of high amounts of moisture by magnesium oxide. Additionally, as magnesium oxide solution is alkaline, it can interact with other medicines, making it incompatible for one-dose packaging. There are three types of magnesium oxide powder formulations: crushed magnesium oxide tablets, magnesium oxide bulk powder, and magnesium oxide granules. However, the appropriate formulation of magnesium oxide for one-dose packages is unknown. The stability of powder formulations stored as one-dose packages at 75% RH for 3 months was evaluated by analyzing the change in weight change and dissolution. The dissolution of the three powder formulations did not change for 3 months. Although the weight had increased in all the formulations, the change in the weight of magnesium oxide granules was the lowest among the three formulations. Isoniazid and levodopa tablets have been reported to interact with magnesium oxide tablets. The incompatibility between the crushed powder of these medicines and magnesium oxide powder formulations was analyzed when stored as one-dose packages. Among the three formulations, incompatibility was the lowest for granule. In conclusion, preserving magnesium oxide and other formulations that causes incompatibility should basically be avoided. However, magnesium oxide granules can be used for storing with the other formulations that interact with magnesium oxide within a term to circumvent incompatibility.

著者

谷澤 久之 佐塚 泰之 小松-芹田 明子 滝野 吉雄

出版者

公益社団法人 日本栄養・食糧学会

雑誌

日本栄養・食糧学会誌 (ISSN:02873516)

巻号頁・発行日

vol.36, no.4, pp.283-289, 1983 (Released:2010-02-22)

参考文献数

12

被引用文献数

8 5

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米酢は米よりつくる醸造酢の一種であり, 単なる食用酢としてではなく, 東洋では民間薬として健康維持に役立ってきた歴史を持っている。著者らは, マウスを用い急性毒性と脂質代謝に及ぼす影響を検討した。その結果, 1) マウスでの急性毒性は21.5ml/kg (p. o.) でその死因は含有する酢酸による上部消化管に対する障害作用に基づくことが認められた。2) 通常食および高コレステロール食で飼育したマウスの血清コレステロール値を米酢は 2.5ml/kg (p. o.) 以上で低下させた。また, 4%酢酸水溶液でも, ほぼ同様の効果が認められた。3) 抗生物質アドリアマイシソによる心臓中の過酸化脂質 (LPO) 上昇に対し, 米酢は2.5ml/kg (p. o.) で抑制した。また, 正常マウス心臓中のLPOも5ml/kg (p. o.) 以上で低下させた。一方, 4%酢酸水溶液のこれらLPOに対する作用は弱いものだった。

著者

松尾 泰佑 富田 隆 工藤 賢三 佐塚 泰之

出版者

一般社団法人日本医療薬学会

雑誌

医療薬学 (ISSN:1346342X)

巻号頁・発行日

vol.44, no.9, pp.464-470, 2018-09-10 (Released:2019-09-10)

参考文献数

8

被引用文献数

6

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Tablet splitting, an important task of the pharmacist, is performed at hospitals and health insurance pharmacies. Although magnesium oxide tablets have no score line, the accuracy of their division is not assured. Furthermore, as magnesium oxide strongly absorbs moisture, the stability of half-tablets is unknown when stored in one-dose packages. The variation and loss when splitting magnesium oxide 500 mg tablets by using a tablet-divider were evaluated. The stabilities of split tablets after storage in one-dose packages at 75%RH were evaluated through the analysis of weight change, disintegration, and dissolution. The variation in half-tablets of magnesium oxide 500 mg tablets was 5.5 ± 4.4% and the loss of almost all tablets was below 1%. Although the weight of the half-tablets increased when preserved in one-dose packages, this was related to the quantity of magnesium oxide stored in one-dose packages and not on the splitting of tablets. The disintegration time of half-tablets was prolonged by increased moisture absorption, but the disintegration time of split tablets was shorter than that of whole tablets when the same quantity of moisture was absorbed. The dissolution rate was similar among split tablets and whole tablets. Magnesium oxide tablets can be administrated as a split tablet, but caution should be exercised regarding prolonged disintegration time. Pharmacists should be careful when using half-tablets in prescriptions.

著者

佐塚 泰之 廣津 祥代 広田 貞雄 金川 麻子 森田 哲生

出版者

日本DDS学会

雑誌

Drug Delivery System (ISSN:09135006)

巻号頁・発行日

vol.13, no.6, pp.415-421, 1998-11-10 (Released:2009-01-21)

参考文献数

19

被引用文献数

1 1

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We prepared the liposomes, changed the entrapped amount of adriamycin (ADR) per the amount of liposome composing lipid, and after the addition of these liposomes with the same concentration of ADR(therefore, different dose of lipid), the tumor cell uptake of ADR was examined. The high entrapped amount of ADR demonstrated the usefulness in the tumor cell uptake of the ADR liposome in vitro. The cell uptake of the liposome depended on additional amount of ADR and liposomal lipid. Next, using ADR contained liposome and irinotecan contained liposome, its usefulness on tumor cell uptake by the polyethyleneglycol (PEG) modification of the surface on the liposome in vitro examined. In both liposome, PEG modification of the surface on the liposome facilitated the initial rate of the liposome uptake into the tumor cell. We have considered that this facilitation was attributed to the lipo-hydrophilic property of PEG and the fixed aqueous layer around the liposome. Therefore, PEG modification of the surface on the liposome, prevents the adhesion of serum opsonine and avoids reticuloendothelial system, does not inhibit tumor cell uptake rather facilitates. From the results of dextran sulfate contained liposome, it is expected that these liposome passed through the membrane of the tumor cell. Therefore, a higher entrapped amount of antitumor agents in the liposome and PEG modification have been confirmed to be beneficial in the tumor cell uptake.

著者

佐塚 泰之 廣津 祥代 宮城島 惇夫 野澤 靖夫 広田 貞雄

出版者

日本DDS学会

雑誌

Drug Delivery System (ISSN:09135006)

巻号頁・発行日

vol.12, no.3, pp.193-198, 1997-05-10 (Released:2009-01-21)

参考文献数

15

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The lipid composition, particle diameter and dose have been reported to affect the liposomal uptake in the tumor. However, effects of encapsulation amount of drugs in liposomes on the uptakes have scarecely been reported either in vitro or in vivo. In this study, we examined the uptake of liposomes containing CPT-11 by Ehrlich ascites carcinoma in vitro changing the encapsulation amount of CPT-11, and also the tissue distribution of liposomal CPT-11 in vivo with mice. After the addition of high concentration as CPT-11, the uptakes in the tumor cell by liposomes was about 1/3 of that by the solution in vitro. However, after the addition of same level as tissue distribution of CPT-11 in vivo, there is no difference on CPT-11 uptakes between liposome and solution. Thus lipid satulation in the tumor cells was observed by increasing liposomes in the medium. For a definite dose, the decrease of CPT-11 amount in the liposomes reduced the uptake in the tumor cell. Therefore, we thought that the uptake of liposomes in the tumor cell depended on lipid amount of liposomes. The increase of CPT-11 amount in the liposomes enhanced CPT-11 concentration in the serum, liver and tumor after administration of liposomal CPT-11 to the mice. An enhanced antitumor activity was expected from the result of SN-38 concentration in the tumor.

著者

藤原 邦彦 大橋 綾子 那谷 耕司 駒野 宏人 西郡 秀夫 中山 貢一 佐塚 泰之 林 宏明 岸本 成史 藤本 康之 田邉 由幸 杉山 晶規 高橋 勝雄 北川 隆之 二井 將光

出版者

日本医療薬学会

雑誌

日本医療薬学会年会講演要旨集

巻号頁・発行日

vol.18, 2008-09-01