著者

Masaki Takigawa Hirofumi Masutomi Yuki Kishimoto Yoshitomo Shimazaki Yoshitomo Hamano Yoshitaka Kondo Tomio Arai Jaewon Lee Toshihiro Ishii Yoshiko Mori Akihito Ishigami

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.40, no.7, pp.975-983, 2017-07-01 (Released:2017-07-01)

参考文献数

21

被引用文献数

1 8

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Vancomycin hydrochloride (VCM) is a glycopeptide antibiotic that is commonly used against methicillin-resistant, Gram-positive cocci despite the nephrotoxic side effects. VCM-induced nephrotoxicity has been reported in 5–28% of recipient patients. Therefore, renal failure induced by VCM has become an important clinical problem. However, the exceedingly complex mechanism of VCM-induced nephrotoxicity is not fully understood. Therefore, this study was designed to clarify time-dependent alterations of VCM-induced nephrotoxicity in mice as a step toward decreasing the risks of kidney injury associated with VCM therapy. VCM was injected intraperitoneally into mice at a dose of 400 mg/kg body weight at 24-h intervals for 3, 5, 7, and 14 d. At 24 h after the last injection, we examined histopathological alterations of the kidney as well as blood biochemistry. VCM administration resulted in a decrease of body weight and increase of kidney weight. Histological examination revealed renal damage such as dilated proximal tubules with occasional casts and interstitial fibrosis in VCM-treated mice. Furthermore, immunohistochemical staining with anti-CD10 and anti-single-stranded DNA antibodies highlighted damaged renal proximal tubules with marked dilatation as well as numerous apoptotic cells as early as day 4 of VCM-treatment. The severity of symptoms progressed until day 15. These results suggest that VCM-induced renal damage and incipient renal failure begin soon after the start of treatment and progressively worsen. This is the first report describing the time-dependence of VCM-induced nephrotoxicity in mice and depicting a model that clarifies the mechanisms of this tissue damage.

著者

Shintaro MAENO Leon DICKS Junichi NAKAGAWA Akihito ENDO

出版者

BMFH Press

雑誌

Bioscience of Microbiota, Food and Health (ISSN:21863342)

巻号頁・発行日

vol.36, no.4, pp.147-149, 2017 (Released:2017-10-04)

参考文献数

10

被引用文献数

15

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Potential fructophilic characteristics of Lactobacillus apinorum, originally isolated from the guts of honeybees (Apis mellifera), were studied in the present study. The species showed typical fructophilic growth characteristics, i.e., active growth on d-fructose, poor growth on d-glucose, and accelerated growth on d-glucose in the presence of electron acceptors. Biochemical characteristics strongly supported classification of the species into fructophilic lactic acid bacteria (FLAB). Furthermore, genetic analyses suggested that the species underwent extensive gene reduction, similar to that recorded for Lactobacillus kunkeei and other FLAB. These data clearly indicated that L. apinorum is the second fructophilic species within the genus Lactobacillus.

著者

Akihito Usui Yusuke Kawasumi Kiyotaka Usui Yuya Ishizuka Kaito Takahashi Masato Funayama Haruo Saito

出版者

東北ジャーナル刊行会

雑誌

The Tohoku Journal of Experimental Medicine (ISSN:00408727)

巻号頁・発行日

vol.242, no.3, pp.183-192, 2017 (Released:2017-07-07)

参考文献数

12

被引用文献数

7

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Traditional autopsy has changed little in the past century. In Japan, the rate of forensic autopsy in cases of unusual death is very low. Therefore, multi-slice computed tomography (CT) has been used to obtain imaging data instead of or in addition to autopsy in suspicious forensic cases. In our institute, postmortem multi-slice CT has been performed since 2009, and by 2014 there were over 1,000 cases. Our extensive experience with postmortem CT shows that in many cases of death by drug overdose, stomach contents exhibit high X-ray absorption. This article reviews the relationship between CT findings of stomach contents and toxicological analysis results in 23 cases of death by drug overdose. All cases (12 females and 11 males, aged 44 ± 11 years) known to have orally ingested drugs were included in this study. We assessed the slices of all stomach areas on consecutive axial CT images. Twenty cases (87%) showed high X-ray absorption in the stomach, while the other three did not demonstrate radio-dense stomach contents even though drug analysis detected lethal concentrations of drugs in the blood. In conclusion, drugs were frequently, but not always, visualized as contents with high X-ray absorption in the stomach. Postmortem gastric CT images can provide useful information in cases of oral drug intoxication if there are empty drug packages or a suicide note at the death scene. However, precise determination of the cause of death requires full autopsy in cases where there is no indication of suicide at the death scene.

著者

Trung Quang PHAM Takayuki HOSHI Yoshihiro TANAKA Akihito SANO

出版者

公益社団法人 計測自動制御学会

雑誌

SICE Journal of Control, Measurement, and System Integration (ISSN:18824889)

巻号頁・発行日

vol.10, no.5, pp.426-432, 2017 (Released:2017-09-23)

参考文献数

24

被引用文献数

2

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Rapid adapting type-I (RA-I) receptor is one type of mechanoreceptors in the human skin. They are believed to be responsible for the detection of stimuli that produce minute skin motion (flutter, slip, microgeometric surface features). The neurophysiological experiments in the paper [J.R. Phillips et al. J. Neurophysiol., Vol. 46, pp. 1192-1203, 1981]raise a question about why the RA-I afferent (innervated into RA-I receptor) fails to represents the stimulus with the width less than 3mm and why their response is anisotropy. It is unclear whether the skin's mechanics or the specific afferent branching of mechanoreceptors themselves are accounted for these phenomena. The present work seeks an interpretation of the neurophysiological phenomena, using a biomechanical finite-element (FE) model with a transduction sub-layer and synthetic sub-model for afferent current. The predicted afferent current matched well with the neural recordings in previous reports. This result suggests a major role of afferent branching in regard to the neurophysiological phenomena.

著者

Masahiro HATTORI Glaezel Angelique TORRES Naoto TANAKA Sanae OKADA Akihito ENDO Junichi NAKAGAWA

出版者

BMFH出版会

雑誌

Bioscience of Microbiota, Food and Health (ISSN:21863342)

巻号頁・発行日

vol.36, no.2, pp.65-72, 2017 (Released:2017-04-19)

参考文献数

23

被引用文献数

4

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Penicillin-binding proteins (PBPs) are responsible for peptidoglycan synthesis. By using biotinylated ampicillin, we detected PBPs of Lactobacillus paracasei strains. Ten PBPs were identified, 7 of which had apparent molecular sizes similar to those of Escherichia coli. In the presence of cholate, strain NRIC 0625 showed an elongated shape, and its putative PBP3 showed cholate-sensitive penicillin-binding activity. Furthermore, this strain was highly sensitive to cefalexin, which is known to inhibit cell division by inactivating PBP3. These results suggest that the septum synthetase PBP3 of lactic acid bacteria can be one of the targets of intestinal bile acid.

著者

Masahiro Tahara Kazuhiro Yatera Kei Yamasaki Takeshi Orihashi Makoto Hirosawa Takaaki Ogoshi Shingo Noguchi Chinatsu Nishida Hiroshi Ishimoto Akihito Yonezawa Junichi Tsukada Hiroshi Mukae

出版者

一般社団法人 日本内科学会

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.55, no.13, pp.1787-1791, 2016-07-01 (Released:2016-07-01)

参考文献数

13

被引用文献数

8

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A 37-year-old woman was admitted to a hospital due to a prolonged fever and a rash on her legs. She had systemic lymphadenitis and a skin abscess on her left leg. Pathological findings of a left leg skin biopsy revealed abscess formation with granulomatous dermatitis, Mycobacterium abscessus complex was cultured from the resected left supraclavicular lymph node, and disseminated M. abscessus complex infection was diagnosed. She was treated with combination treatment with antimicrobials and percutaneous drainage, and her clinical findings improved. Four months later, she developed acute lymphocytic leukemia. Leukemia is a risk factor for disseminated M. abscessus complex infection, even before developing leukemia.

著者

Koichi Yoneyama Atsuko Sekiguchi Takashi Matsushima Rieko Kawase Akihito Nakai Hirobumi Asakura Toshiyuki Takeshita

出版者

日本医科大学医学会

雑誌

Journal of Nippon Medical School (ISSN:13454676)

巻号頁・発行日

vol.83, no.1, pp.6-14, 2016-01-15 (Released:2016-03-09)

参考文献数

19

被引用文献数

2

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Aim: The aim of the present study was to elucidate the clinical characteristics of pregnancy-associated maternal deaths. Methods: We performed a retrospective analysis with medical records and autopsy reports of cases of pregnancy-associated deaths. We collected information on all maternal deaths related to pregnancy that occurred in 3 hospitals affiliated with Nippon Medical School in Japan from January 1, 1984, to December 31, 2014. Data analyzed were maternal age, past medical history, parity, gestational age, clinical signs and symptoms, cause of death, and maternal autopsy findings. Results: A total of 26 maternal deaths occurred during the 31-year study period. Autopsies were performed for 16 patients (61.5%). The 26 deaths included 19 (73.1%) classified as direct maternal deaths and 7 (26.9%) classified as indirect maternal deaths. The mean maternal age at death was 33.1±4.3 years (range, 26-41 years). The highest percentage of women was aged 35 to 39 years (38.5%). Of the 26 maternal deaths, 69% occurred at 32 to 41 weeks of gestation. In cases of direct maternal death, the leading causes were amniotic fluid embolism (7 cases, 27.0% of all deaths) and hemorrhage (6 cases, 23.1% of all deaths). In cases of indirect obstetric deaths, the causes included cardiovascular disorders, cerebrovascular disorders, sepsis due to group A streptococcal infection, and hepatic failure of unknown etiology. Conclusions: Amniotic fluid embolism was the leading cause of maternal deaths and was followed by obstetric hemorrhage. To prevent and reduce the number of maternal deaths in Japan, further basic and clinical research on amniotic fluid embolism is required.

著者

Akihito Endo Sanae Okada Hidetoshi Morita

出版者

公益財団法人 応用微生物学・分子細胞生物学研究奨励会

雑誌

The Journal of General and Applied Microbiology (ISSN:00221260)

巻号頁・発行日

vol.53, no.3, pp.191-200, 2007 (Released:2007-08-29)

参考文献数

37

被引用文献数

8 30 32

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Diversity and compositions of the Lactobacillus, Streptococcus, and Bifidobacterium group in the feces of six healthy, actively racing horses (Thoroughbreds) were analyzed by using PCR-denaturing gradient gel electrophoresis (DGGE) and real-time PCR with primer sets specific for each group. PCR-DGGE analysis of the feces showed that Lactobacillus equi, Lactobacillus johnsonii, a phylogenetic relative of Lactobacillus salivarius, a phylogenetic relative of Lactobacillus gastricus, and Weissella confusa were predominant in almost all of the feces tested, and Streptococcus bovis/Streptococcus equinus was predominant in the Streptococcus group. The Bifidobacterium group was not detected by single-PCR but atypical species of the group were found in three of the six Thoroughbreds tested by nested-PCR. Calculation and estimation of lactic acid bacteria and bifidobacteria revealed that lactic acid bacteria were predominant in the feces and bifidobacteria were minor. These results indicate that the community of lactic acid bacteria and bifidobacteria in horse feces are unique because of the presence of specific species for horse feces and a minority of the Bifidobacterium group. Repeated tests of the feces from the same horse over 3 months showed that the diversity and composition of lactic acid bacteria and bifidobacteria in the feces was basically stable throughout the test period.

著者

AYA SAKAGUCHI AKINOBU KADOKURA PETER STEIER KAZUYA TANAKA YOSHIO TAKAHASHI HARUKA CHIGA AKIHITO MATSUSHIMA SATORU NAKASHIMA YUICHI ONDA

出版者

GEOCHEMICAL SOCIETY OF JAPAN

雑誌

GEOCHEMICAL JOURNAL (ISSN:00167002)

巻号頁・発行日

vol.46, no.4, pp.355-360, 2012-08-20 (Released:2013-11-08)

参考文献数

26

被引用文献数

90 27

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Concentrations of the radionuclides, U, Pu, and Cs were measured in water samples (10-20 L) to study analyte dispersion and migration following the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident. A total of 8 water samples including oceanic water and paddy-field water were collected in the vicinity of the plant. Determinations of U, Pu and Cs isotopes were performed by accelerator mass spectrometry (AMS), inductively coupled plasma mass spectrometry (ICP-MS), and γ-ray spectrometry. The 236U/238U atom ratio was in the range 1.83-8.20 × 10-9 for fresh water and around 0.57 × 10-9 for seawater while the concentration of 236U was about 104-105 and 106 atoms/kg, respectively. Plutonium (239,240Pu) was detected in one riverine sample and the marine samples at very low levels and with large uncertainty. The concentrations of 137Cs in fresh riverine samples were 0.02-0.46 Bq/kg which are more than three orders of magnitude larger than the global fallout level. As for seawater samples within 80 km offshore of the FDNPP, the concentrations of 137Cs were 10-20 times higher than that of the Japan Sea water. Also 134Cs and 137Cs were of similar concentrations in all samples. The results show that volatile and refractory nuclides such as Cs, U and Pu exist in the dissolved phase, which can be readily assimilated by plants/humans. However the environmental impact of Pu and U in the vicinity of the FDNPP is considered to be low in comparison to that of the volatile radionuclide Cs.

著者

立川 陽仁 Tachikawa Akihito

出版者

三重大学人文学部文化学科

雑誌

人文論叢 (ISSN:02897253)

巻号頁・発行日

no.27, pp.191-204, 2010

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カナダの太平洋沿岸部において、20世紀を通じて地域を支える一大産業にまで成長したサケ漁業は、現地の先住民社会の経済的な自立も支えてきた。しかし1990年代からのサケ漁業の衰退により、先住民社会は経済的自立を支える新たな方途を模索せねばならなくなっている。そこで一部の先住民に注目されたのが、養殖業であった。養殖業に注目した先住民の一部は、みずからのコミュニティを再び活性化させることに成功しているが、彼らはそれだけに飽き足らず、みずからの成功を他の先住民社会にまで拡大しようと目論んでいる。そのような先住民有志によって設立されたのが、先住民養殖業協会である。本稿は、この先住民養殖業協会の設立背景と現時点における活動を整理し、かつその将来像についても若干の分析をおこなうものである。

著者

Ohnishi Hiroshi Yokoyama Akihito Yasuhara Yoshifumi Watanabe Akira Naka Tetsuji Hamada Hironobu Abe Masahiro Nishimura Kazutaka Higaki Jitsuo Ikezoe Junpei Kohno Nobuoki

出版者

BMJ

雑誌

Thorax (ISSN:00406376)

巻号頁・発行日

vol.58, pp.872-875, 2003

被引用文献数

16 104

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Background: The circulating level of KL-6/MUC1 is a sensitive marker for various interstitial lung diseases. Previous case reports have suggested that KL-6 may also be increased in some patients with drug induced pneumonitis. A study was undertaken to determine whether serum KL-6 could be a marker for particular types of drug induced pneumonitis. Methods: The findings of high resolution computed tomographic (HRCT) chest scans of 30 patients with drug induced pneumonitis were reviewed separately by two independent observers. The pneumonitis was classified into four predominant patterns: widespread bilateral consolidation (diffuse alveolar damage, DAD; n=7), fibrosis with or without consolidation (chronic interstitial pneumonia, CIP; n=11), consolidation without fibrosis (bronchiolitis obliterans organising pneumonia or eosinophilic pneumonia, BOOP/EP; n=8), and diffuse ground glass opacities without fibrosis (hypersensitivity pneumonitis, HP; n=4). Serum KL-6 levels were measured by a sandwich enzyme linked immunosorbent assay. Results: The overall sensitivity of serum KL-6 in detecting drug induced lung disease was 53.3%, which was lower than its sensitivity in detecting other interstitial lung diseases. However, the KL-6 level was increased in most patients with a DAD or CIP pattern (16/18; 88.9%) and was closely correlated with their clinical course. In contrast, serum KL-6 levels were within the normal range in all patients with a BOOP/EP or HP pattern. Conclusions: Particular patterns detected by HRCT scanning, such as DAD and CIP but not the BOOP/EP or HP patterns, are associated with increased circulating KL-6 levels in drug induced pneumonitis. Serum KL-6 levels may reflect the clinical activity of the particular disorders.

著者

Akihito Konishi Yasukazu Hirao Kouzou Matsumoto Hiroyuki Kurata Takashi Kubo

出版者

(社)日本化学会

雑誌

Chemistry Letters (ISSN:03667022)

巻号頁・発行日

vol.42, no.6, pp.592-594, 2013-06-05 (Released:2013-06-05)

参考文献数

50

被引用文献数

52

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The improved Scholl reaction allows for the direct cyclization of anthracene oligomers to give bisanthene, teranthene, and quateranthene. Furthermore, a variety of π-expanded bisanthenes are obtained by the Diels–Alder cycloaddition of bisanthene with several arynes. These reactions would allow us to synthesize various size- and shape-controlled polyperiacenes.