著者

Hiroshi IWANAGA Hirohiko TSUZUKI Yoichiro KAMIYAMA Hiroshi UEDA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.25, no.9, pp.1101-1106, 2009-09-10 (Released:2009-09-10)

参考文献数

13

被引用文献数

1 2 3

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With a specific and strong molecular-recognition capability cultivated in humoral acquired immunity, an antibody has been extensively utilized in various applications, such as diagnostics and therapy. However, so far most of its uses have been limited to be in the liquid phase. In view of its potential uses, such as a gas-phase biosensor or a high-performance air filter, we have tried to verify a previously undescribed binding reaction between protein antigens and corresponding antibodies immobilized on a solid surface by using fluorescence resonance energy transfer between the two. Our data showed that the antibody on a solid surface specifically reacted with a protein antigen supplied from the gas phase under the normal ambient condition. Also discovered was that the reaction occurred even faster than that in the liquid phase under several assay conditions.

著者

Hiroshi Ueda Hiroshi Itoh Junya Hirai Kiyotaka Hidaka

出版者

The Plankton Society of Japan, The Japanese Association of Benthology

雑誌

Plankton and Benthos Research (ISSN:18808247)

巻号頁・発行日

vol.17, no.2, pp.221-230, 2022-05-30 (Released:2022-05-31)

参考文献数

22

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The Paracalanus parvus (Claus, 1863) species complex is a common marine calanoid copepod found in the world’s oceans. Recent genetic analyses of its specimens from the world oceans revealed that the complex in the western North Pacific consists of three species, i.e., P. indicus Wolfenden, 1905, P. tropicus Andronov, 1977 and an undescribed species. We assign the last one to be Paracalanus orientalis n. sp. by comparing it with previous morphological descriptions of related species. Both sexes of the new species are fully described based on genetically identified specimens from the surface layer of the south of Japan. The new species can be reliably distinguished from P. indicus and P. tropicus by the hunchback shape of the female if it is clearly present. Among the three species, the high length:width ratio of the third exopod segment of leg 4 is also characteristic of the new species, which would be identifiable, in most cases, by having a ratio of 5.4 or more.

著者

Ko Yamamoto Hiroki Shiomi Takeshi Morimoto Hiroki Watanabe Akiyoshi Miyazawa Kyohei Yamaji Masanobu Ohya Sunao Nakamura Satoru Mitomo Satoru Suwa Takenori Domei Shojiro Tatsushima Koh Ono Hiroki Sakamoto Kiyotaka Shimamura Masataka Shigetoshi Ryoji Taniguchi Yuji Nishimoto Hideki Okayama Kensho Matsuda Kenji Nakatsuma Yohei Takayama Jun Kuribara Hidekuni Kirigaya Kohei Yoneda Yuta Imai Umihiko Kaneko Hiroshi Ueda Kota Komiyama Naotaka Okamoto Satoru Sasaki Kengo Tanabe Mitsuru Abe Kiyoshi Hibi Kazushige Kadota Kenji Ando Takeshi Kimura on behalf of the OPTIVUS-Complex PCI Investigators

出版者

The Japanese Circulation Society

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-22-0837, (Released:2023-03-11)

参考文献数

11

被引用文献数

5

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Background: There is a paucity of data on the effect of optimal intravascular ultrasound (IVUS)-guided percutaneous coronary intervention (PCI) compared with standard PCI or coronary artery bypass grafting (CABG) in patients with multivessel disease.Methods and Results: The OPTIVUS-Complex PCI study multivessel cohort was a prospective multicenter single-arm study enrolling 1,021 patients undergoing multivessel PCI including the left anterior descending coronary artery using IVUS aiming to meet the prespecified criteria for optimal stent expansion. We conducted propensity score matching analyses between the OPTIVUS group and historical PCI or CABG control groups from the CREDO-Kyoto registry cohort-3 (1,565 and 899 patients) fulfilling the inclusion criteria for this study. The primary endpoint was a composite of death, myocardial infarction, stroke, or any coronary revascularization. In the propensity score-matched cohort (OPTIVUS vs. historical PCI control: 926 patients in each group; OPTIVUS vs. historical CABG control: 436 patients in each group), the cumulative 1-year incidence of the primary endpoint was significantly lower in the OPTIVUS group than in the historical PCI control group (10.4% vs. 23.3%; log-rank P<0.001) or the historical CABG control group (11.8% vs. 16.5%; log-rank P=0.02).Conclusions: IVUS-guided PCI targeting the OPTIVUS criteria combined with contemporary clinical practice was associated with superior clinical outcomes at 1 year compared with not only the historical PCI control, but also the historical CABG control.

著者

Roberta Beltrão Michiko Monde Takayuki Mine Hiroshi Ueda

出版者

The Plankton Society of Japan, The Japanese Association of Benthology

雑誌

Plankton and Benthos Research (ISSN:18808247)

巻号頁・発行日

vol.18, no.2, pp.63-73, 2023-05-31 (Released:2023-05-30)

参考文献数

45

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Ariake Bay has Japan’s biggest nori seaweed farming field in the innermost part and has suffered from environmental degradation since the late 1990s. To elucidate the seasonal patterns in the copepod community structure in the inner bay, we examined monthly plankton-net samples collected in the top 2-m layer at 11 stations for 5 years from April 2002 to March 2007. Of the 11 stations, 7 were at the high-turbidity site (HTS) representing the nori farming field and 4 in the offshore low-turbidity site (LTS) for comparison. The copepod community in the HTS was dominated almost year round by Oithona davisae, whose 5-year mean abundance of 203 ind. L−1 comprised 84% of copepods and was more than double that in the LTS. O. davisae became extremely abundant in summer with the maximum monthly mean abundance in the HTS of 2756 ind. L−1 and the maximum sample-specific abundance of 10116 ind. L−1. The following predominant copepods in the HTS were Parvocalanus crassirostris in summer–autumn, Microsetella norvegica in summer, Acartia omorii in spring, and Paracalanus parvus s.l. irregularly. Possible causes for high abundance of O. davisae in the HTS are assumed to be sufficient food resources, water temperature over 21°C, and a wide salinity tolerance range of the species.

著者

Noriko Tsubamoto-Sano Junji Ohtani Hiroshi Ueda Masato Kaku Kazuo Tanne Kotaro Tanimoto

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.61, no.1, pp.119-124, 2019 (Released:2019-03-28)

参考文献数

34

被引用文献数

4

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This study was to investigate the influences of habitual mouth breathing on memory and learning ability during the growth period. At age 5 weeks, the experimental rats were subjected to surgery to close completely one side of the nasal cavity. An 8-arm radial maze was used to evaluate memory and learning ability at age 7, 11, and 15 weeks. Moreover, the brain was extracted at age 7, 11, and 15 weeks, and subjected to histomorphometric examination for the distribution and number of pyramidal cells in the hippocampal CA1 and CA3 regions after Nissl staining. The trial time to accomplish each task was significantly longer in the experimental group than in the control group throughout the experimental period. The number of pyramidal cells was significantly less in the experimental rats than in controls in both the CA1 and CA3 regions for the entire experimental period. Thus, the functional deterioration of the respiratory system during the growth phase exerts a substantial effect on the growth and development of the central nervous system.

著者

Sakiko Orui Sakaguchi Hiroshi Ueda

出版者

The Plankton Society of Japan, The Japanese Association of Benthology

雑誌

Plankton and Benthos Research (ISSN:18808247)

巻号頁・発行日

vol.13, no.4, pp.173-179, 2018-11-21 (Released:2018-11-14)

参考文献数

22

被引用文献数

6

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Pseudodiaptomus inopinus, a common estuarine calanoid copepod in the Northwest Pacific, has been suggested to be a species complex. The population of the mainland of Japan consists of two allopatric forms that are distinguishable by the length of the posterior processes of the genital operculum; the forms with short and long processes are tentatively called SP- and LP-forms, respectively. We analyzed the mitochondrial cytochrome c oxidase subunit I (COI) gene and the nuclear ribosomal internal transcribed spacer 1 (ITS1) gene of 34 individuals (12 SP- and 22 LP-forms) collected from various localities. The genetic differences between the two forms were 12–15% for COI and 7–10% for ITS1. They were clearly separated into different clades in both COI and ITS1 trees. These results confirmed that the two forms belong to different species. The SP-form is a distinct species because the shape of the genital operculum of P. inopinus s. str. has long posterior processes as in the LP-form. Pseudodiaptomus japonicus Kikuchi, 1928, which is the SP-form and was once synonymized with P. inopinus, is revived. The genetic comparison using available sequence data indicates that Pseudodiaptomus koreanus Soh et al., 2012 is the same species as P. japonicus.

著者

Zheng Wang Akira Ando Atsuko Takeuchi Hiroshi Ueda

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

vol.24, no.3, pp.421-425, 2018 (Released:2018-07-25)

参考文献数

31

被引用文献数

1 3

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This study sought to determine the optimal cooking conditions under which lutein would be maintained while oxalic acid and nitrate ion would be removed. The effects of cooking temperature and time control on the concentration of oxalic acid, nitrate ion, and lutein in spinach were investigated. The results demonstrated that boiling at 100°C for 2 min was the optimal cooking condition. This preserved approximately 77% of lutein while 67% of oxalic acid and 30% of nitrate ion were removed. If nitrate ion is disregarded, we also found that submerging in hot water at 60°C for 30 s was another optimal condition. In this case, 96% of lutein remained, 28% of oxalic acid was removed, whereas the content of nitrate ion was unchanged.

著者

Hiroshi Ueda Sakiko Orui Sakaguchi

出版者

The Plankton Society of Japan, The Japanese Association of Benthology

雑誌

Plankton and Benthos Research (ISSN:18808247)

巻号頁・発行日

vol.14, no.1, pp.29-38, 2019-02-27 (Released:2019-03-12)

参考文献数

26

被引用文献数

3

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The brackish-water calanoid copepod known as Pseudodiaptomus inopinus in the mainland of Japan consists of two genetically separate species. One is P. japonicus, which was once synonymized with P. inopinus but was recently revived. This paper describes the other species as Pseudodiaptomus yamato n. sp., which is confirmed to have morphological differences from P. inopinus s.s. based on specimens from the type locality (Lake Taihu, China) of the latter. We also redescribe P. japonicus and P. inopinus s.s. for comparative purposes. The three species are distinguishable by the combination of the following morphologies: 1) weak or prominent posterior round projections of the female last pediger; 2) relative length of posterior processes of the female genital operculum; 3) presence or absence of medial spinules on the first exopodal segment of the female leg 5; and 4) the size of spinules at the center of the ventral surface of the male second urosomite. Significant inter-population variation is observed in some spinules of P. japonicus. The past and present records indicate that Pseudodiaptomus yamato n. sp. is endemic to Japan and confined to the coasts affected by the warm Kuroshio Current from western Kyushu to the middle of Honshu, while P. japonicus is widespread in northern East Asia without overlapping the range of P. yamato n. sp. The range of Pseudodiaptomus inopinus s.s. most certainly does not extend to those of P. yamato n. sp. and P. japonicus.

著者

Hiroshi Ueda

出版者

The Plankton Society of Japan, The Japanese Association of Benthology

雑誌

Plankton and Benthos Research (ISSN:18808247)

巻号頁・発行日

vol.13, no.1, pp.28-31, 2018-02-28 (Released:2018-03-14)

参考文献数

14

被引用文献数

1

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High-concentration pure glycerol has been used as a medium for permanent preservation of micro-crustacean specimens. However, glycerol-mounted permanent slide preparations are rare because of the difficulty in sealing the coverslip over the long term. I devised a glycerol-mounted permanent preparation method, especially for a small dissected piece of a micro-crustacean using a glass depression slide and a coverslip with secure sealing, in order to overcome some of the disadvantages of the popular permanent mountants that solidify, such as discoloration of specimens and difficulty in remounting. Unique characteristics of the preparation method are: (1) the central glycerol being of a few millimeters in diameter, which embeds the specimen and is retained in the depression center by a paraffin wax circle painted on the depression bottom; (2) the marginal glycerol, which fills up the clearance between the glass slide and the coverslip and prevents intrusion of the sealant into the clearance; (3) sealing with nail polish first and Canada balsam second, where the latter can seal securely over the long term and the former, being a fast-drying sealant, prevents intrusion of the latter into the marginal glycerol. By following this method, even a small dissected piece can be easily found under a microscope and taken out from the preparation for detailed examination with a low risk of going missing. The procedure to make the preparation is presented in detail.

著者

Minoru Ijichi Gyo Itani Hiroshi Ueda

出版者

日本プランクトン学会、日本ベントス学会

雑誌

Plankton and Benthos Research (ISSN:18808247)

巻号頁・発行日

vol.12, no.3, pp.145-150, 2017-08-22 (Released:2017-08-24)

参考文献数

26

被引用文献数

4

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The life cycle and precopulatory mate guarding behavior of the poecilostomatoid copepod Goidelia japonica associated with the spoon worm Urechis unicinctus (Echiura) are described based on specimens from 19 worms collected from western Japan. A total of 676 copepods were collected from the host’s rectum (494 copepods) and body surface (182 copepods). Copepod numbers were significantly correlated with host weight. Copepods in the rectum consisted almost completely of copepodids (35%) and adult males (65%), with only one non-ovigerous adult female recorded. In contrast, those on the body surface were all adult males (24%) or females (76%), of which 58% were ovigerous. No nauplii or CI occurred. In the rectum, 36% of the adult males guarded 67% of the copepodids, consisting of CII–CV. From these results, their life cycle is considered as follows: nauplii and CI live as plankton; just before or after molting to CII, they enter the host’s rectum and females are probably soon guarded by males; they grow up to CV there; CV females guarded by males move to the body surface just before the final molt; on the body surface females molt to adults and spawn eggs. Guarding the first symbiotic stage (CII) is a unique precopulatory behavior for symbiotic poecilostomatoid copepods. This can be explained from the viewpoint of evolutionary resolution of the intersexual conflict and advantage for early copepodids in precopula.

著者

Yuta Yamanouchi Akira Ando Yuko Takano-Ishikawa Hiroshi Ueda

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

pp.FSTR-D-22-00186, (Released:2023-08-08)

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Brassicaceae vegetables contain glucosinolate (GSL), which is well known for its role in the plant defense system, bitter substances, and functional components. This study reported the GSL profiles of 18 GSLs in 14 Brassicaceae vegetables (kale, cabbage, kohlrabi, broccoli, cauliflower, komatsuna, pak choi, turnip, choy sum, Chinese cabbage, rutabaga, leaf mustard, leaf radish, and radish root) consisting of 64 cultivars grown in Japan. The GSL compositions showed specific trends among each species, and furthermore, there was considerable variation in the total and individual GSL contents among the cultivars and tissues of the same vegetable. Because each vegetable was grown under the same conditions, this variation was expected to primarily be due to the genetic background. It is desirable to collect additional data from commonly distributed cultivars to conduct more detailed studies on GSLs in Brassicaceae vegetables, such as investigating a negative correlation between GSL consumption and cancer risk.

著者

Yoshitaka HARADA Yuki OHMURO-MATSUYAMA Mika TSUNA Hiroshi UEDA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.37, no.3, pp.455-459, 2021-03-10 (Released:2021-03-10)

参考文献数

13

被引用文献数

4

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Immunochromatography assay is an easy and rapid on-site detection method. However, conventional sandwich immunochromatographies using two antibodies can only detect target molecules above a threshold size. Small molecules below 1000 in molecular weight are usually detected using competitive immunoassay. However, competitive immunoassay is not suitable for visual detection of low concentration samples. Based on the principles of open sandwich immunoassay, which detects small molecules via interchain interaction of separated variable region fragments (VH and VL) from a single antibody, we developed non-competitive open sandwich immunochromatography. Bone Gla protein (BGP)-C7, a peptide containing the seven C-terminal amino acids of human osteocalcin, was selected as the target. By using VL fragments fixed on a nitrocellulose membrane, and colored cellulose bead-labeled VH fragments, we specifically detected 10 ng/mL of BGP-C7. This is the first report of open sandwich immunochromatography, which is an easy and rapid method for on-site, signal-on detection of small molecules.

著者

Hiroshi Ueda Chikako Yamazaki Masatoshi Yamazaki

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.25, no.9, pp.1197-1202, 2002 (Released:2002-09-01)

参考文献数

24

被引用文献数

169 207

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Oral administration of the perilla leaf extract (PLE) to mice inhibits inflammation, allergic response, and tumor necrosis factor-α production. We also found that PLE suppressed the tumor necrosis factor-α (TNF-α) production in vitro. Using the inhibitory activity of TNF-α production in vitro as the index for isolation, we searched the active constituents from PLE and isolated luteolin, rosmarinic acid and caffeic acid as active components. Among the isolated compounds, only luteolin showed in vivo activity: inhibition of serum tumor necrosis factor-α production, inhibition of arachidonic acid-induced ear edema, inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced ear edema and inhibition of oxazolone-induced allergic edema. These results suggest that luteolin is a genuinely active constituent which is accountable for the oral effects of perilla.

著者

Hiroshi UEDA Atsuko TAKEUCHI Tadayuki WAKO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.77, no.9, pp.1809-1813, 2013-09-23 (Released:2013-09-23)

参考文献数

23

被引用文献数

11

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Bunching onion [Allium fistulosum L. (Liliaceae)] secretes mucus in the cavities of its green leaves. The effects of the mucus, which is consumed as food, were examined. The mucus augmented the production of tumor necrosis factor (TNF)-α and monocyte chemotactic protein (MCP)-1 from RAW 264 cells and of interleukin (IL)-12 from J774.1 cells; however, extracts from green leaves and white sheaths did not. An oral administration of this mucus to mice augmented the immune functions of peritoneal cells by increasing TNF-α and IL-12 production and phagocytosis. It also augmented interferon (IFN)-γ production from spleen cells and natural killer (NK) activity. These results suggest that an oral administration of the A. fistulosum mucus can enhance natural immunity.

著者

Hiroshi Ueda

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.42, no.11, pp.1773-1782, 2019-11-01 (Released:2019-11-01)

参考文献数

67

被引用文献数

4 10

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Currently, only a few medicines have been approved for use in the clinical treatment of chronic pain, but they are not fully satisfying due to their side effects. From the view that radical treatment, rather than simply treating symptoms, is more important in addressing life-long chronic pain, we have been investigating translational research for a mechanism-based medicine to treat pain. Through the characterization of various types of peripheral and central neuropathic pain in mice, we discovered that lysophosphatidic acid (LPA) plays roles in definitive mechanisms of the development and maintenance of neuropathic pain. We found LPA1 receptor- and LPA3 receptor-mediated amplification of LPA production could be a key mechanism underlying the initiation and maintenance of this pain. We have developed stress-induced fibromyalgia models, and have revealed that LPA1 receptor-signaling also plays key roles in the mechanism. Throughout these studies, we found that LPA plays a key role in pain memory, and that LPA1 receptor- and LPA3 receptor-antagonists could reverse the established pain, and thereby cure the disease source of pain.

著者

Hiroshi Ueda Hidefumi Nagai

出版者

The Japanese Society of Systematic Zoology

雑誌

Species Diversity (ISSN:13421670)

巻号頁・発行日

vol.17, no.1, pp.97-107, 2012-05-25 (Released:2018-03-30)

参考文献数

27

被引用文献数

2 2

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Two new species of cyclopoid copepod, Halicyclops setiformis and H. itohi, are described based on female specimens from the Tama River estuary in Tokyo Bay, central Japan. One of the defining characteristics of H. setiformis is the presence of a setiform lateral element on the third exopodal segment of legs 3 and 4. Among its congeners, only H. blachei Lindberg, 1952 has similar elements; H. setiformis differs from it in the shape of the third endopodal segment of leg 4. This new species is considered carnivorous based on the occurrence of a specimen biting a nematode and the presence of stout, claw-like spines on the oral appendages. The second new species, Halicyclops itohi, is distinguishable from most of its congeners in the absence of lateral protuberances on the genital double-somite, the absence of a terminal accessory seta on the caudal ramus, and a 3.4.4.3 spine formula on the third exopodal segment of legs 1-4. Congeners sharing these three morphological characteristics differ variously from H. itohi in their caudal ramus shape, urosomal frills, antennal setal number, medial elements of the third endopodal segment of leg 4, and/or the shape of leg 5. The hitherto known Halicyclops species from Japan are briefly reviewed, and a key to the Japanese species is presented to resolve previous questionable identifications.

著者

Aiko Mitsuzawa Hiroyuki Miyamoto Hiroshi Ueda

出版者

日本プランクトン学会、日本ベントス学会

雑誌

Plankton and Benthos Research (ISSN:18808247)

巻号頁・発行日

vol.12, no.2, pp.115-122, 2017-05-30 (Released:2017-06-06)

参考文献数

29

被引用文献数

14

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Copepod nauplii are the primary food item for early-stage marine fish larvae, but their feeding selectivity on the nauplii of different copepod species has not been well studied. This study revealed feeding selectivity in the dominant preflexion larvae of three fishes Sebastiscus marmoratus, Sebastes sp. and Ammodytes japonicus in the Seto Inland Sea, Japan, in spring and winter. Copepod nauplii and eggs numerically constituted 80–91% of the prey items in their guts. Chesson’s preference index for the nauplii of various copepod species was positive for Calanus sinicus and Paracalanus parvus sensu lato, negative for Acartia omorii, and negative or neutral for Oithona similis. This result is probably attributable to the size and swimming behavior of the prey species. The preference index in A. japonicus larvae was not significantly different between the nauplii of different species, suggesting that the larvae are less selective than the other two fishes. The preference index for Calanus eggs was very high, especially in Sebastiscus marmoratus and Sebastes sp., and most of the eggs in the guts were shrunken, probably due to digestion. In terms of volume, C. sinicus nauplii and eggs in gut contents represented 36–72% of total copepod nauplii and >80% of total copepod eggs, respectively, because of high selectivity by fish larvae and their large individual volumes compared with the dominant copepods. This indicates that C. sinicus is the most important prey species for early fish larvae despite their low abundance in the environment.

著者

Tatsuya SUZUKI Yuriko MUNAKATA Kazuki MORITA Tatsuya SHINODA Hiroshi UEDA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.23, no.1, pp.65-70, 2007 (Released:2007-01-10)

参考文献数

29

被引用文献数

23 41

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Zearalenone (ZEA) is an estrogenic mycotoxin produced by Fusarium sp., and its production on corn and small grains during storage has been of considerable concern. For sensitive ZEA detection, we applied an open sandwich (OS) immunoassay that can noncompetitively detect monovalent antigens utilizing an antigen-induced enhancement of the VH/VL interaction. We cloned the VH and VL cDNAs of anti-ZEA mAb to a split-Fv phagemid pKST2, and firstly both VH and VL fragments were displayed on M13 phage p9 and p7, respectively, using an amber suppressor, TG-1, as a host. The split-Fv phage showed specific binding to immobilized ZEA, which was well inhibited by free ZEA. Then, the VH/VL interaction and its antigen-dependency were analyzed using a non-suppressor HB2151 as a host to produce VH-displaying phage and his/myc-tagged soluble VL in the culture supernatant. By capturing VL with an anti-myc or -his antibody and probing bound VH-phage, ZEA was successfully detected with a superior detection limit as well as a wider working range than those of a competitive assay. Also, essentially the same results were reproduced with purified VH-alkaline phosphatase and MBP-VL fusion proteins.