著者

Lea KRUMP Ríona G. SAYERS Emer KENNEDY Jim O’MAHONY Gearóid P. SAYERS

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.83, no.11, pp.1715-1721, 2021 (Released:2021-11-16)

参考文献数

35

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An observational study was conducted to comparatively assess the efficacy of three different oral rehydration and buffering solutions, differentiated by their strong ion difference (SID) concentration, for treatment of neonatal calves with naturally acquired diarrhea. The SID concentrations tested were 100 mM, 170 mM and 230 mM for treatments SID100, SID170 and SID230, respectively. Clinical assessment and blood gas analysis were completed for 18 diarrheic calves once pre- and twice post- (6 and 24 hr after) oral administration with one of the three treatments. A repeated measure mixed model approach was used to analyze (a) the within-group efficacy of each treatment over time and (b) the between-group comparison at each timepoint. SID230 treatment resulted in a significant increase in blood pH, HCO3−, BE, SID and Na+ at 6 and 24 hr after treatment, and a significant decrease in AG and K+ by 24 hr after treatment. There were no significant changes in any of the blood gas parameters after treatment with SID100 and SID170. SID230 treatment also resulted in blood gas parameter changes that were significantly different to the other two groups. These results suggest that the optimum SID concentration for the treatment of calves with diarrhea is likely to be higher than current recommendations.

著者

Gennaro ALTAMURA Bianca CUCCARO Claudia ELENI Carina STROHMAYER Sabine BRANDT Giuseppe BORZACCHIELLO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.22-0060, (Released:2022-04-15)

被引用文献数

7

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Recent evidence suggests a possible association of Felis catus papillomavirus type 2 (FcaPV-2) DNA with feline oral squamous cell carcinoma (FOSCC). In this study, type-specific PCR targeting two genes (L1/E6 or E1/E6) of FcaPV-1/-2/-3/-4/-5/-6 was performed to detect viral DNA in a large amount of FOSCC samples collected in Italy and Austria. FcaPV-1/-2/-3/-4/-5 were detected in 7/113 (6.2%), 7/93 (7.5%), 6/113 (5.3%), 1/113 (0.9%) and 2/113 (1.8%) specimens, respectively, with different prevalences in Italian vs Austrian samples, whilst FcaPV-6 went undetected. Our results confirms that FcaPV-2 is the most prevalent in FOSCC, followed by FcaPV-1/-3 and suggest that FcaPVs have variable circulation rates in European countries.

著者

Jiyoung PARK Aryung NAM Hae-Beom LEE Seong Mok JEONG Dae-Hyun KIM

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0673, (Released:2022-04-15)

被引用文献数

1

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A 10-year-old intact male Shih Tzu dog presented with hematuria. Double-contrast cystography revealed a polypoid filling defect at the apex of the urinary bladder. Ultrasonography revealed a heterogeneously hypoechoic intramural mass with minimal vascular flow beneath the submucosal layer. After partial cystectomy, a well-demarcated bladder leiomyosarcoma was diagnosed on histopathology. The patient was alive and well without any clinical signs, recurrence, or metastasis at the 29-month follow-up after the surgical removal of the bladder mass. Leiomyosarcoma should be considered as a differential diagnosis if mass-like lesions are observed in the urinary bladder, although this type of malignancy is rare in canines. Histopathological confirmation is important for predicting prognosis and determining further medical plans.

著者

Yan Qing JIA Xiang Wei WANG Xi CHEN Xin Xin QIU Xing Long WANG Zeng Qi YANG

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.84, no.3, pp.473-483, 2022 (Released:2022-03-30)

参考文献数

30

被引用文献数

5

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Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by interferon (IFN)-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading frame (ORF) of 1,152 bp encoding a protein of 384 amino acids containing two conserved Nmi/IFI35 domain (NID) motifs. Tissue distribution analysis of chIFI35 in healthy and Newcastle disease (ND) virus-infected chickens indicated a positive correlation between chIFI35 mRNA transcription and ND viral loads in various tissues. The role of chIFI35 in regulation NDV replication were further assessed by up- or down-regulated chIFI35 expression in DF-1 cells transfected with plasmid harboring chIFI35, pCMV-3HA-chIFI35 or shRNA targeting chIFI35, pshRNA-chIFI35 plasmids. NDV replications in DF-1 cells were significantly reduced or slightly increased by over- or under-expression of the chIFI35 protein, respectively, indicating the role of chIFI35 in anti-NDV infection. Moreover, chIFI35 also involved in regulation of viral gene transcription and IFNs expression. The collected data were meaningful for research of chicken antiviral immunity and shed light on the pleiotropic antiviral effect of chIFI35 during NDV infection.

著者

Naoki ISOBE Tomoyasu KUROSE Naoki SUZUKI Tomoko KOSHIISHI Kazutoshi UENO Keiichi HISAEDA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.84, no.1, pp.59-63, 2022 (Released:2022-01-07)

参考文献数

20

被引用文献数

3

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The present study was undertaken to examine whether oral administration of colostrum to mastitic cows reduced inflammation in the udder. Fifty milliliters of a colostrum whey product was administered orally daily for 3 days to cows suffering from mastitis. Milk was collected on day 0 and 7 of colostrum administration. For Experiment 1, milk from 11 udder quarters with high somatic cell counts (SCC) in four cows was used. SCC in milk decreased significantly after colostrum administration, whereas colostrum administration increased sodium and IgA concentrations significantly compared with those before administration. In Experiment 2, cows with clinical mastitis were divided into two groups, with and without colostrum administration, whereas all cows with subclinical mastitis were administered colostrum. Antibiotics were infused into the mammary gland from the first day of colostrum administration for 2–4 days. There was no significant decrease in SCC after colostrum administration in any group. However, udder firmness in both clinical mastitis groups was reduced after administration regardless of colostrum administration. IgA concentration in both clinical mastitis groups was significantly increased after colostrum administration compared to that before administration, although there was no significant difference between them. These results suggest the possibility that oral administration of colostrum attenuates inflammation of the mammary gland. Further studies are required to examine the effect of colostrum more precisely using cows with subclinical and chronic mastitis and longer duration of colostrum administration.

著者

Yoshio KIKU Yuya NAGASAWA Fuyuko TANABE Kazue SUGAWARA Atsushi WATANABE Eiji HATA Tomomi OZAWA Kei-ichi NAKAJIMA Toshiro ARAI Tomohito HAYASHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.78, no.9, pp.1505-1510, 2016 (Released:2016-10-01)

参考文献数

27

被引用文献数

15 18

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Staphylococcus aureus (SA) is a major cause of bovine mastitis, but its pathogenic mechanism remains poorly understood. To evaluate the role of lipoteichoic acid (LTA) in the immune or inflammatory response of SA mastitis, we investigated the gene expression profile in bovine mammary epithelial cells stimulated with LTA alone or with formalin-killed SA (FKSA) using cap analysis of gene expression. Seven common differentially expressed genes related to immune or inflammatory mediators were up-regulated under both LTA and FKSA stimulations. Three of these genes encode chemokines (IL-8, CXCL6 and CCL2) functioning as chemoattractant molecules for neutrophils and macrophages. These results suggest that the initial inflammatory response of SA infection in mammary gland may be related with LTA induced chemokine genes.

著者

Fumitaka TAKAHASHI Takaharu HAKOZAKI Nobuo KANNO Shuji SUZUKI Yasuji HARADA Satoshi SOETA Shinichi NAKAMURA Shinya YAMAGUCHI Yasushi HARA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0315, (Released:2022-04-05)

被引用文献数

1

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We evaluated the completeness of bony fusion of the atlantoaxial joint (AAJ) through polymethylmethacrylate fixation (PMF) and atlantoaxial plate fixation (APF) using six canine models with dens partial resection. In both groups, the hydroxyapatite content at the AAJ was measured up to 7 months postoperatively using quantitative computed tomography. Histological assessment revealed fibrous fusion in the PMF group. Meanwhile, in the APF group, only one dog achieved fibrous fusion, whereas the remaining three showed bony fusion. To our knowledge, this study was the first to evaluate AAJ fusion histologically after PMF and APF. The present study demonstrates that PMF and APF may stabilize the AAJ without clinical complications. Therefore, PMF and APF are clinically useful fixation methods for atlantoaxial instability.

著者

Taiki KIDA Arisa YAMAZAKI Koji KOBAYASHI Tatsuro NAKAMURA Takayuki NAKAGAWA Ryohei NISHIMURA Takahisa MURATA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.22-0020, (Released:2022-03-23)

被引用文献数

2

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Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases.

著者

Takuro NAGAHARA Koichi OHNO Itsuma NAGAO Taisuke NAKAGAWA Yuko GOTO-KOSHINO Masaya TSUBOI James K. CHAMBERS Kazuyuki UCHIDA Hirotaka TOMIYASU Hajime TSUJIMOTO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0257, (Released:2022-03-14)

被引用文献数

1

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Intestinal lymphangiectasia (IL) is often observed in dogs with chronic small intestinal diseases. Hypoplasia of the lymphatic vessel due to decreased lymphangiogenesis, which has been suggested in human idiopathic IL, may contribute to the pathogenesis of canine IL. This study aimed to evaluate the diameter and number of lymphatic vessels in full-thickness small intestinal specimens of dogs with IL. Immunohistochemical labeling of lymphatic endothelial cell markers was performed on retrospectively retrieved full-thickness small intestinal specimens. Sixteen dogs with histologically confirmed IL were included, of which 10 had lymphoplasmacytic enteritis (LPE), and six had granulomatous lymphangitis (GL). Nine dogs that died from non-gastrointestinal disorders and with little or no abnormalities in the small intestine were used as controls. Lymphatic vessel diameters in dogs with IL were significantly increased in all layers of the small intestine, including the villus lacteal, lamina propria, submucosa, muscularis, and mesentery, compared with controls (all P<0.01). There was no significant difference in the lymphatic vessel diameters between dogs with LPE and GL (all P>0.05). There was no significant difference in the number of lymphatic vessels between dogs with IL and the controls in all layers of the small intestine (all P>0.05). This study demonstrated that IL was observed in all layers of the small intestine, including the submucosa, muscularis, and mesentery, independent of the underlying disease. Factors other than reduced lymphatic vessels would contribute to the pathogenesis of IL in dogs.

著者

Wang Hsien-Chi Lee Shu-Hwae Chang Tien-Jye WONG Min-Liang

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.66, no.7, pp.855-859, 2004-07-25

参考文献数

9

被引用文献数

9

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牛海綿状脳症(狂牛病)のような遅発性神経病は,動物による汚染飼料の摂取によって伝達されることがある.したがって,市販飼料中の肉成分を検査することは,こうした疾病をコントロールする上で公衆衛生上重要なことである.そこで,PCR-RFLP法を用いて,犬用飼料と猫用飼料の肉成分の検査を行った.動物ミトコンドリア・チトクロームb(cytb, CYT)遺伝子のヌクレオチド配列(359塩基対)をPCRで増幅してから,制限酵素AluIまたはMboIで切断する方法により,台湾で市販されている犬用飼料と猫用飼料各8銘柄について検査を行った.その結果,犬用飼料については全銘柄が,犬以外の3種類以上の動物種(牛,豚,山羊,鶏)由来の肉を含むこと,猫用飼料については5銘柄が鶏肉を含むことが認められた.

著者

Yui HIROSE Mona UCHIDA Masaya TSUBOI Takayuki NAKAGAWA Leo YAGA Shingo MAEDA Yasuyuki MOMOI Yugo KURIKI Mako KAMIYA Yasuteru URANO Tomohiro YONEZAWA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.22-0003, (Released:2022-03-07)

被引用文献数

3

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Since gamma-glutamyl transpeptidase (GGT) is highly and locally expressed in human breast cancer, a GGT-enzymatically activatable fluorescent probe, gamma-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), has been developed to detect the human breast cancer area with high performance. In this study, GGT expression and the efficacy of gGlu-HMRG on visualization were investigated in canine mammary gland tumors (MGT). Seventeen non-fixed fresh-frozen MGT specimens and each peritumoral control tissue were utilized. The GGT mRNA levels were highly observed in the tumor specimens compared with the control. GGT immunostaining was mostly observed on the cell membrane and cytosol of the alveolar and duct mammary epithelium of MGT tissues. These signals were strongly positive in several cases while they were mild to not observed in other cases. When gGlu-HMRG solution was dropped to the non-fixed tissue pieces of MGT or control tissues, the fluorescence intensities (FIs) were measured using Maestro in-vivo imaging device. FIs in MGT tissues were significantly higher than each control tissue 20 min after treatment. Based on Youden index method said that the maximum sensitivity and specificity of FI was 82.4% and 82.4%. These findings suggest that GGT is highly expressed in several MGTs in dogs and gGlu-HMRG could visualize at least a part of MGT tissues in dogs. Nevertheless, it should be needed to assess the false-negative areas more carefully in canine than human cases.

著者

Kiyoshi NAKAUCHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.61, no.1, pp.63-65, 1999-01-25 (Released:2000-04-12)

参考文献数

28

被引用文献数

34 56

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A total of 375 fecal samples of 56 mammalian species belonging to 17 families of 4 orders were examined for the detection of Balantidium coli from December 1994 to August 1995. As a result, B. coli was found from 6 species belonging to 4 families of 2 orders (Primates and Artiodactyla) of host animals examined. White-handed gibbon (Hylobates lar), squirrel monkey (Saimiri sciurea) and Japanese macaque (Macaca fuscata) were new hosts for B. coli. All the wild boar (Sus scrofa) and chimpanzee (Pan troglodytes) examined were positive. The highest number of B. coli was obtained from a chimpanzee (1, 230/g feces). No B. coli was detected from the animals of orders Rodentia and Carnivora including dogs and cats. The rarity of B. coli infection in breeding animals in Japan suggests that there is no serious problem in controlling infections.

著者

Hiroshi SHIMODA Seiji TAMARU Masahiro MORIMOTO Toshiharu HAYASHI Masayuki SHIMOJIMA Ken MAEDA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.9, pp.1241-1242, 2011 (Released:2011-09-30)

参考文献数

11

被引用文献数

11 15

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A previous serosurvey of Japanese encephalitis virus (JEV) among dogs suggested that dogs are well suited for use as sentinels for assessing the risk of JEV transmission to humans. To examine the clinical symptoms and duration of anti-JEV antibodies in dogs, three dogs were experimentally challenged with JEV. All JEV-infected dogs did not show any clinical signs or abnormal blood tests, except for C-reactive protein. Virus-neutralization titers rapidly increased and were maintained until 70 days postinfection, and neither the virus nor the viral genome was detected in blood. Thus, since dogs live in close proximity to humans as companion animals, they are well suited for use as sentinels for surveying the human risk of JEV infection.

著者

Tetsuya SHIMODA Mika TANABE Yuuki SHOJI Takuya KANDA Kouhei KISHIDA Ai KISHIDA Atsushi HASHIMOTO Mako OTSUKA Akina MIYAMOTO Tomoko KAWAKITA Wataru OSHITA Reijiro HIRAO Akihisa SUWA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.84, no.2, pp.251-256, 2022 (Released:2022-02-23)

参考文献数

22

被引用文献数

2

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A cat was presented with depression and anorexia. The complete blood cell count (CBC) revealed non-regenerative anemia (PCV, 8.5%), marked thrombocytopenia (2,400/µl), and leukocytosis (32,090/µl). In the peripheral blood, proliferation of blast cells (85%; 27,276/µl) and basophils (7.7%; 2,460/µl) was observed. Bone marrow aspirate showed hyperplasia with 8.8% blasts and 90.2% basophils of all nucleated cells. The blast cells were negative for myeloperoxidase staining and positive for alpha-naphthol butyrate esterase staining, indicating the agranular blasts are monoblasts. Thus, acute monoblastic leukemia (M5a) with chronic basophilic leukemia was diagnosed. Basophils accounted for more than 40% of the bone marrow, and we diagnosed secondary basophilic leukemia. Secondary basophilic leukemia should be included in the differential list when abnormal basophil increases are observed in feline bone marrow.

著者

松野 年美 針口 二三男 岡本 勉

出版者

社団法人日本獣医学会

雑誌

日本獣医学雑誌 (ISSN:09167250)

巻号頁・発行日

vol.53, no.1, pp.p13-17, 1991-02

被引用文献数

1

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古典的な抗マラリア剤パマキンおよびプリマキンなどの8-aminoquinolineの抗コクシジウム活性については, 未だに報告されていない. それら2薬剤につきバタリー試験により, 5種のニワトリコクシジウム : Eimeria tenella, E. necatrix, E. acervulina, E. maxima, およびE. brunetti (いずれも実験室標準株) に対する活性を調べた. 両薬剤は共にE. tenella, E. necatrixに対してのみ有効で, その他のコクシジウムに対しては, 全く無効であった. 前2者に対する有効性については, E. tenellaに対してパマキンは, 飼料中125-250ppmの投与により顕著に症状を抑制する効果を示し, プリマキンは31.2ppm以上の投与で一層優れた抑制効果を発揮したほか, E. necatrixに対しては, パマキンは250ppmで, プリマキンは125ppm以上の濃度で症状の抑制効果を発揮した. これらの試験でパマキンは125-250ppmの濃度で明らかにヒナの増体重を抑制する傾向を示したが, プリマキンは, 500ppm投与の例を除き250ppmまでの濃度ではそのような傾向を示さなかった. また, パマキンのbenzophenone, nitropyrazole, dinitrobenzoie acid, quinoline類との分子化合物ならびに硫酸塩, 亜鉛塩などにつき同様のバタリー試験を行ったところ, それらの化合物はパマキン自身が示した抗コクシジウム活性を失うことなく, 体重増を低下させる影響を著しく軽度なものとし, 広い安全域を示した. 本来強い抗マラリア・抗ピロプラズマ作用のある8-aminoquinoline類に対して感受性を示したE. tenellaやE. necatrixは, 生活環に上皮細胞寄生のほか特に中はい葉由来細胞に寄生する時期を持つという意味でマラリアやピロプラズマのような住血原虫の生活態度に似ており, 他の3種の感受性を示さなかったEimeriaとは異なっている. 本試験結果はこれら8-aminoquimoline感受性の胞子虫の間には生理機構の中に互いに類似した要素のあることを示唆しているのかもしれない.

著者

松田 敬一 大塚 浩通 一條 俊浩 川村 清市

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.68, no.1, pp.75-77, 2006-01-25

参考文献数

23

被引用文献数

3

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バナナ給与が子牛の免疫細胞に及ぼす影響を調査した.バナナ(2g/kg BW)を5日間経口給与して0, 5, 10および15日目に白血球表面抗原の解析を行った.5日目において給与群のCD^<3+>, CD^<3+>CD45R^-およびCD^<3+>TcR^+細胞数は対照群に比較して有意に増加した(P<0.05).以上のように, 子牛へのバナナ給与はTリンパ球を増加させることから, 感染防御能を高める可能性が考えられた.

著者

折野 宏一 山本 晋二 渡辺 清隆

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.55, no.5, pp.785-787, 1993-10-15 (Released:2008-02-15)

参考文献数

12

被引用文献数

21 22

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酵素免疫測定法により測定したウマ血漿および血清フェリチン濃度は血漿の方が血清より低かった. しかしながら, 血漿と血清を75℃で15分間加熱処理することにより, 両者のフェリチン濃度は同レベルに上昇した. これらの結果はウマ血漿にはフェリチンの免疫測定を阻害する特有のフェリチン結合タンパク質が存在することを示唆する. ウマ血清フェリチン濃度は血清へのウマフィブリノーゲン添加により減少した. フィブリノーゲンはウマ脾臓フェリチンの免疫測定をも阻害し, またこれと結合した. これらの結果から, ウマフィブリノーゲンはフェリチンの免疫測定を阻害するフェリチン結合タンパク質の一つであると結論された.

著者

Mizuki TAKEDA Nobuaki ARAI Yuzo KOKETSU Yasushi MIZOGUCHI

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0266, (Released:2022-01-18)

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To assess factors for canine skin extensibility, our study investigated associations between the dogs’ skin extension index and the following factors, gender, age, neuter status, weight, coat color and six coat color related gene polymorphisms. Swab samples were collected from 69 toy poodles to extract DNA. The skin extension indices of the lower back and the neck were measured using the following formula: vertical height of the skin fold divided by body length multiplied by 100. The dogs’ age, weight, gender, neuter status and coat color were also recorded, as well as polymorphisms of the following six selected coat color related genes, Melanocortin 1 receptor, Tyrosinase-related protein 1, Melanophilin, Canine β-defensin-1, Major Facilitator Superfamily Domain Containing 12 and Agouti-signaling protein (ASIP). Univariable analysis showed there was a meaningful association between the lower back skin extension index and both gender and age (P<0.001 and P=0.048, respectively). Also, there was a possible association between the lower back skin extension index and ASIP Single nucleotide polymorphism (SNP) (R96C) (P=0.078). Linear model analysis showed there was a significant association between the lower back skin extension index and gender (P<0.001), and there was a tendency of the association between the lower back skin extension index and ASIP SNP (R96C) (P=0.098). In addition, there was an association between gender and age for the skin extension index. (P=0.048). Therefore, these results suggest that a greater risk of skin extensibility in toy poodle could be related to being female and the ASIP SNP (R96C), because these factors were associated with higher lower back skin extension index.

著者

Hirosumi MIYAKAWA Huai-Hsun HSU Mizuki OGAWA Yuichi MIYAGAWA Naoyuki TAKEMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0566, (Released:2021-12-30)

被引用文献数

1

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This study compared canine and feline fibroblast growth factor (FGF)-23 concentration measurements between automated chemiluminescence assay (CLEIA) and enzyme-linked immunosorbent assay (ELISA). Seventy serum samples each from dogs and cats were evaluated. FGF-23 measurements by CLEIA significantly correlated with those of ELISA in both dogs and cats. The Bland–Altman test showed that FGF-23 between CLEIA and ELISA had fixed and proportional biases, respectively, in both dogs and cats. Measurements by CLEIA were lower than those of ELISA, especially in higher serum FGF-23 concentrations. This study showed that FGF-23 concentrations in dogs and cats can be evaluated by automated CLEIA. However, FGF-23 cannot be directly compared between CLEIA and ELISA.

著者

Haruka KATAOKA Nanami KOITA Natsuko Ito KONDO Hiroshi C. ITO Momoko NAKAJIMA Kunikazu MOMOSE Hiroko IIMA Tomoo YOSHINO Tomoko AMANO Takio KITAZAWA Daiji ENDOH Nobuyoshi NAKAJIMA Hiroki TERAOKA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0516, (Released:2022-01-18)

被引用文献数

4

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The red-crowned crane Grus japonensis in Hokkaido, Japan forms a closed population as a residence that is independent of the mainland population. Based on observations of a limited number of individuals as well as cranes in captivity, red-crowned cranes are omnivores and eat fish, worms, insects and plants in their own territories except in winter, when they are fed with dent corn that is supplied in eastern Hokkaido. DNA metabarcoding based on high throughput sequencing was carried out using universal primer sets for cytochrome oxidase subunit I gene. Feces from 27 chicks collected in June and July in the period from 2016 to 2018 and intestinal contents from 33 adult and subadult cranes that were found dead almost throughout year in 2006–2013 in the field in eastern Hokkaido were used. Although compositions varied considerably in the cranes, both insects and fish were found in adults and subadults to the same extents, while insects were predominant in chicks. Both insects and fish were detected in all seasons for adults and subadults. Horse flies, scarab beetles and weevils accounted for the most of the insects regardless of the life stage. Dace, stickleback, flatfish and sculpin were the major fish species in adults, while chicks ate almost only stickleback. The results provide the first comprehensive data on carnivorous diets in wild red-crowned cranes in eastern Hokkaido as basis for conservation of red-crowned cranes, for which the life style and area continuing to change.