著者

Go SUGAHARA Akio KIUCHI Reiko USUI Ryouichi USUI Takayuki MINESHIGE Junichi KAMIIE Kinji SHIROTA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0449, (Released:2013-11-08)

被引用文献数

5

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To the best of our knowledge, we present here the first report of a case involving granulomatous pododermatitis caused by Fusarium proliferatum in a 10-year-old female cat. A cutaneous mass developed on the foot-pad of the right hind leg. Nodular granulomatous dermatitis with numerous macrophages and multinucleated giant cells containing cytoplasmic fungal structures were revealed on histological examination. Periodic acid-Schiff reaction and Fungi-Fluor staining clearly revealed irregular, septate fungal hyphae englobed by macrophages and multinucleated giant cells. Polymerase chain reaction and sequence analysis targeting three domains of the extracted fungal DNA revealed 100% amplicon homology with F. proliferatum.

著者

黄 禹錫 金 賢一 李 殷松 林 正黙 廬 湘鎬 申 泰英 黄 光南 李 柄千

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.62, no.1, pp.1-5, s・viii, 2000-01

被引用文献数

8

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血清無添加で,"HanWoo"(Bos taurus coreanae)の卵子を牛卵管上皮細胞(BOEC)と共培養し,産生された組織増殖抑制因子metalloproteinase(TIMP)-1の精製純化と,胚の発育効果を調べることを目的とした.TIMP-1はHPLC-System(Perseptive Biosystems Inc.,U.S.A)とゲルろ過法により精製純化し,分子量はSDA-Page(Bio-Rad Co.,U.S.A)により32kDa蛋白質であることが明らかとなった.培養BOECのTIMP-1局在を,isothiocyanate蛍光染色標本で証明した.血清無添加培養液にTIMP-1を,0,1.25,2.5ならびに5μg/mlの割合で加え,体外受精により得られた胚の発育を観察した.この結果,2.5μg/ml添加で桑実胚と胚盤胞への発育効果を認めた.今回の試験から,BOECはTIMP-1を産生し,この糖蛋白質は"HanWoo"(Bos taurus coreanae)体外受精卵の発育を桑実胚と胚盤胞へ促進した.

著者

Masaaki KATAYAMA Yoshiki KAWAKAMI Rieko KATAYAMA Shunsuke SHIMAMURA Yasuhiko OKAMURA Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0209, (Released:2013-11-05)

被引用文献数

3

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Clarithromycin (CLM) has been known to increase the cyclosporine (CsA) trough level in human and feline organ transplant patients. However, the interaction of CLM with CsA has not been reported in dogs. In this study, the effects of multiple dosing of CLM on the pharmacokinetics of CsA in three healthy beagles were investigated. The treatments included CsA 10 mg/kg alone and CsA 10 mg/kg + multiple-dose of CLM 10 mg/kg. Co-administration of CLM with CsA resulted in significant increases of oral bioavailability of CsA. The results of our study suggest that administration of multiple therapeutic doses of CLM may decrease the required CsA dosage in CsA-based immunosuppressive therapy in renal transplanted dogs.

著者

Kenji ISHIWATA Tomonori MINAGAWA Tsunesuke KAJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.60, no.8, pp.911-917, 1998 (Released:2001-10-06)

参考文献数

35

被引用文献数

11 23

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The clinical effects of recombinant feline interferon-ω (rFeIFN-ω), produced in silkworm by recombinant baculovirus, were examined in 3-4 month-old beagle dogs given an experimental canine parvovirus type-2 (CPV-2) infection. Clinical symptoms, such as pyrexia, vomiting, anorexia and diarrhea, were observed on day 4 after oral inoculation of 107 TCID 50 of CPV-2 (cc 238 strain) in almost all the inoculated dogs. From day 4, rFeIFN-ω (1 mega units/kg/day) or physiological saline was administered intravenously to infected dogs for 3 consecutive days. Seven out of 17 dogs treated with physiological saline showed hemorrhagic diarrhea and continuously expressed severe clinical enteritis; one dog died with a large amount of hemorrhagic rice-water stool on day 6 after viral exposure. In contrast, 4 out of 12 dogs treated with rFeIFN-ω showed severe clinical enteritis associated with intermittent diarrhea. Scoring of fecal condition revealed that treatment with rFeIFN-ω significantly shifted the enteritis from a severe to mild form. Furthermore, rFeIFN-ω administered in the morning decreased the number of dogs expressing clinical enteritis in the evening suggesting a rapid effect. Vomiting and anorexia were also improved by treatment with rFeIFN-ω. These results suggest that rFeIFN-ω can reduce severe enteritis caused by CPV-2 infection in dogs.

著者

村上 昇 幸野 亮太 中原 桂子 井田 隆徳 黒田 治門

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.62, no.7, pp.S・viii, 763-766, 2000-07-25

被引用文献数

3

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一年以上の間, 室温22度, 14時間明:10時間暗の照明条件下で飼育されたシマリスを短日照明(10時間明:14時間暗)と低温条件に暴露することにより季節外冬眠を誘起した.我々は一年のどの時期でもこの季節外冬眠を誘起できた.この季節外冬眠は季節間において, 冬眠一党醒インターバルや, それぞれの冬眠や覚醒時間には有意な差を認めなかったが, 冬眠に入るまでの期間の長さにおいて夏のみ約60日を要し, 他の季節の平均30日より長かった.さらに, 覚醒インターバルでの覚醒時刻は冬では明期に起こるのに対し, 春では明期と暗期でほぼ等しい割合で起こった.これらの結果はシマリスの冬眠がサーカディアンリズム(概日リズム)とサーカニュアルリズム(概年リズム)の両者にリンクしていることを示唆している.夏の季節外冬眠において, セロトニン枯渇剤であるパラクロロフェニルアラニン(PCPA)の冬眠中での慢性投与は冬眠を阻止し, 非冬眠動物への投与は逆に冬眠を誘発した.一方, オピオイドのアンタゴニストであるナロキソンの投与は覚醒時間の延長を起こした.これらの結果は, セロトニンによる冬眠誘超や維持機構がサーカニュアル(概年リズム)システムと独立したものであることを示唆している.

著者

Mohammad Mahbubur RAHMAN Akira YABUKI Moeko KOHYAMA Sawane MITANI Keijiro MIZUKAMI Mohammad Mejbah UDDIN Hye-Sook CHANG Kazuya KUSHIDA Miori KISHIMOTO Remi YAMABE Osamu YAMATO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0443, (Released:2013-10-25)

被引用文献数

1 7

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GM2 gangliosidosis variant 0 (Sandhoff disease, SD) is a fatal, progressive neurodegenerative lysosomal storage disease caused by mutations of the HEXB gene. In canine SD, a pathogenic mutation (c.283delG) of the canine HEXB gene has been identified in toy poodles. In the present study, a TaqMan probe-based real-time PCR genotyping assay was developed and evaluated for rapid and large-scale genotyping and screening for this mutation. Furthermore, a genotyping survey was carried out in a population of toy poodles in Japan to determine the current mutant allele frequency. The real-time PCR assay clearly showed all genotypes of canine SD. The assay was suitable for large-scale survey as well as diagnosis, because of its high throughput and rapidity. The genotyping survey demonstrated a carrier frequency of 0.2%, suggesting that the current mutant allele frequency is low in Japan. However, there may be population stratification in different places, because of the founder effect by some carriers. Therefore, this new assay will be useful for the prevention and control of SD in toy poodles.

著者

Sungjin CHOI I-Li LIU Kenichi YAMAMOTO Muneki HONNAMI Takamasa SAKAI Shinsuke OHBA Ryosuke ECHIGO Shigeki SUZUKI Ryouhei NISHIMURA Ung-Il CHUNG Nobuo SASAKI Manabu MOCHIZUKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0054, (Released:2013-10-25)

被引用文献数

3 8

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We investigated biodegradability and new bone formation after implantation of tetrapod-shaped granular artificial bone (Tetrabone®) or β-tricalcium phosphate granules (β-TCP) in experimental critical-size defects in dogs, which were created through medial and lateral femoral condyles. The defect was packed with Tetrabone® (Tetrabone group) or β-TCP (β-TCP group) or received no implant (control group). Computed tomography (CT) was performed at 0, 4 and 8 weeks after implantation. Micro-CT and histological analysis were conducted to measure the non-osseous tissue rate and the area and distribution of new bone tissue in the defect at 8 weeks after implantation. On CT, β-TCP was gradually resorbed, while Tetrabone® showed minimal resorption at 8 weeks after implantation. On micro-CT, non-osseous tissue rate of the control group was significantly higher compared with the β-TCP and Tetrabone groups (P<0.01), and that of the β-TCP group was significantly higher compared with the Tetrabone group (P<0.05). On histology, area of new bone tissue of the β-TCP group was significantly greater than those of the Tetrabone and control groups (P<0.05), and new bone distribution of the Tetrabone group was significantly greater than those of the β-TCP and control groups (P<0.05). These results indicate differences in biodegradability and connectivity of intergranule pore structure between study samples. In conclusion, Tetrabone® may be superior for the repair of large bone defects in dogs.

著者

Pichanun LINHARATTANARUKSA Sayamon SRISUWATANASAGUL Suppawiwat PONGLOWHAPAN Muhammad KHALID Kaywalee CHATDARONG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0114, (Released:2013-10-22)

被引用文献数

1 4

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The extracellular matrix of the cervix that comprises collagen, elastin, proteoglycans and glycosaminoglycans (GAGs) is thought to have an essential role in cervical relaxation. This study investigated the proportion of collagen and smooth muscle as well as the GAGs in cervices obtained from healthy bitches at different stages of the estrous cycle and bitches with open- and closed-cervix pyometra. Cervices were collected after ovariohysterectomy. The proportion of collagen to smooth muscle was determined using Masson’s trichrome staining. Alcian blue staining was used to evaluate the relative distribution of cervical GAGs. The proportion of cervical collagen relative to smooth muscle was higher at estrus compared to anestrus (P≤0.05). It was also higher (P≤0.05) in bitches with open- compared to those with closed-cervix pyometra. Overall, hyaluronan (HA) was the predominant GAG in the canine cervix. In the luminal epithelium, the staining intensity for HA was stronger in estrus than in anestrus (P≤0.05), but not in diestrus (P>0.05). On the contrary, the intensity for the combined keratan sulfate (KS) and heparan sulfate (HS) was stronger in anestrus than in estrus and diestrus (P≤0.05). In bitches with pyometra, the staining intensity of the stroma for KS and HS was weaker in open- compared to closed-cervix pyometra (P≤0.05). Collectively, the different profiles of collagen and GAG suggest that the metabolism of both collagen and GAGs in the canine cervix is associated with hormonal statuses during the estrous cycle and cervical patency of bitches with pathological uterine conditions, such as pyometra.

著者

Jae Yeon LEE Myung Cheol KIM

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0062, (Released:2013-10-09)

被引用文献数

5 35

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The present study evaluated and compared the oxidative stress status of dogs undergoing laparoscopic or open ovariectomy. Twelve healthy female dogs were divided into two groups according to the type of the surgical procedure, laparoscopic or open ovariectomy. Plasma total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) levels for the evaluation of oxidative stress were determined. Increases in plasma TOS and OSI levels and decreases in TAS levels were observed in both groups after surgery. The TOS level was significantly lower in the laparoscopic ovariectomy group compared with the open surgery group. Laparoscopic ovariectomy is a safe and beneficial surgical alternative to traditional ovariectomy with respect to oxidative stress status in dogs.

著者

Shiho USUI Yasushi MIZOGUCHI Hidemi YASUDA Nobuaki ARAI Yuzo KOKETSU

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0369, (Released:2013-10-08)

被引用文献数

1 10

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The objectives of this study were to set specific dog breed and sex standards for total cholesterol (T-Cho) and total triglyceride (T-TG) concentrations in dogs and to quantify the associations between dog age and concentrations of both lipids for different breeds. Increased age was associated with higher T-Cho and T-TG concentrations in all five breed groups (P<0.05); T-Cho concentrations increased by 62.5 mg/dL between 9 and 16 years of age, and T-TG concentrations increased by 4.8 mg/dL per year of age (P<0.05). Miniature Schnauzers had the highest T-Cho concentrations of the studied breeds, while Miniature Dachshunds had the lowest concentrations (P<0.05). Veterinarians should consider dog age and breed when they use the lipid concentrations for diagnostic purposes.

著者

Yusuke MURAHATA Asami YAMAMOTO Yuya MIKI Yoshiaki HIKASA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0398, (Released:2013-10-08)

被引用文献数

1 3

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This study aimed to investigate and compare the antagonistic effects of atipamezole, yohimbine and prazosin on medetomidine-induced diuresis in healthy cats. Five cats were repeatedly used in each of the 9 groups. One group was not medicated. Cats in the other groups received 40 µg/kg medetomidine intramuscularly and saline (as the control), 160 µg/kg prazosin, or 40, 160 or 480 µg/kg atipamezole or yohimbine intravenously 0.5 hr later. Volume, pH and specific gravity of urine; plasma arginine vasopressin (AVP) level; and creatinine, osmolality and electrolyte levels in both urine and plasma were measured. Both atipamezole and yohimbine, but not prazosin, antagonized medetomidine-induced diuresis. The antidiuretic effect of atipamezole was more potent than that of yohimbine, but was not dose dependent, in contrast to the effect of yohimbine at the tested doses. Both atipamezole and yohimbine reversed medetomidine-induced decreases in both urine specific gravity and osmolality and increases in plasma osmolality and free-water clearance. Antidiuresis of either atipamezole or yohimbine was not related to the area under the curve for AVP level, although the highest dose of both atipamezole and yohimbine initially and temporarily increased plasma AVP levels, suggesting that this may partly influence the antidiuretic effects of both agents. The diuretic effect of medetomidine in cats may be mediated by α2-adrenoceptors, but not α1-adrenoceptors. Atipamezole and yohimbine can be used as antagonistic agents against medetomidine-induced diuresis in healthy cats.

著者

鈴木 正嗣 梶 光一 山中 正実 大泰司 紀之

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.58, no.6, pp.505-509, 1996-06-25

参考文献数

29

被引用文献数

13

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北海道東部産のエゾシカ胎子87例で胎齢を推定し, 受胎日の変異と外部形態の発達過程とを明らかにした. 胎子の体重(W)と胎齢(T)との間には, T=(√^3<W>+2.730)/0.091関係式が認められた. この式を用いて胎齢を算出し, 捕殺日からの逆算により求められた推定受胎日は, 10月7日から翌1月17日の範囲で変異していた. しかし, その多くは10月中旬から11月上旬にかけて集中していた. また, 11月下旬以降に受胎したと思われる胎子9例のうち, 6例が1歳メスから採取された標本であった. これは, 北海道東部個体群の良好な栄養状態が, 1歳メスの成長と性成熟を冬期間にも可能にすることを示唆している. 胎子の外部形態においては, 感覚毛や一般被毛の発現時期と白斑の発現時期とが重複していなかった. また, いくつかの発達過程上の変化が, 特定の体重で起こることも確認された. これらの特性を利用することにより, 胎子成長は4段階のステージに分割できた. 各ステージにおける胎子の外部形態的特徴は, エゾシカ以外のニホンジカでも簡便な胎齢推定に役立つと考えられる.

著者

Shi-Xia ZHANG Hong-Bin WANG Jian-Tao ZHANG Nan ZHANG Long PAN

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.9, pp.1161-1166, 2013 (Released:2013-10-01)

参考文献数

26

被引用文献数

1 10

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Colopexy was accomplished in eight healthy mixed-breed dogs by use of a 3-portal laparoscopic technique without major intraoperative and postoperative complications. A permanent adhesion between the colon and the abdominal wall was observed. Concentrations of acute-phase C-reactive protein (CRP) were measured in serum as a marker of systemic inflammation postoperatively, and no relevant increase in CRP concentrations was found.

著者

Yanyan CHENG Jiangdong XUE Chunyu YAO Li GAO Dexing MA Yan LIU Zhigang ZHANG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.9, pp.1139-1146, 2013 (Released:2013-10-01)

参考文献数

47

被引用文献数

3 7

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The objective of this study was to evaluate the possible protection of resveratrol against lung injury induced by arsenic trioxide (As2O3). Twenty-four healthy Chinese Dragon Li cats of either sex were randomly divided into four groups: control (1 ml/kg physiological saline), As2O3 (1 mg/kg), resveratrol (3 mg/kg) and resveratrol (3 mg/kg) + As2O3 (1 mg/kg). The resveratrol + As2O3- treated group was given resveratrol 1 hr before As2O3 (1 mg/kg) administration. We found that pretreatment with resveratrol in a clinically comparable dose regimen can reversed the changes in morphological and biochemical parameters induced by As2O3 in the lung. Resveratrol treatment also upregulated the activities of antioxidant enzymes and attenuated As2O3-induced increases in reactive oxygen species, 8-hydroxydeoxyguanosine and malondialdehyde production in the lung. In addition, resveratrol attenuated the As2O3-induced reduction in the ratio of reduced glutathione to oxidized glutathione, the content of total glutathione and lung arsenic burden. These findings indicated that resveratrol can provide significant protection against As2O3-induced oxidative damage.

著者

Masanori KOBAYASHI Tatsuya HORI Eiichi KAWAKAMI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.9, pp.1249-1252, 2013 (Released:2013-10-01)

参考文献数

20

被引用文献数

2 7

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In healthy male dogs, peripheral plasma testosterone (T), prostaglandin E2 (PGE2) and seminal plasma PGE2 levels were measured before, during and after ejaculation, and semen quality was examined after oral administration of PGE2. Plasma T and PGE2 levels did not change during these periods, but the seminal plasma PGE2 level of combined the first and second fractions was significantly higher than those at 0–5 and 5–10 min after the start of ejaculation of the third fraction. Semen volume but not quality increased after PGE2 administration. In conclusion, large amounts of PGE2 are released from the prostate gland during the early part of ejaculation, and PGE2 plays an essential role in secretion of seminal plasma.

著者

Hiroki KAWABATA Ai TAKANO Teruki KADOSAKA Hiromi FUJITA Yoshiki NITTA Mutsuyo GOKUDEN Toshiro HONDA Junko TOMIDA Yoshiaki KAWAMURA Toshiyuki MASUZAWA Fubito ISHIGURO Nobuhiro TAKADA Yasuhiro YANO Masako ANDOH Shuji ANDO Kozue SATO Hideyuki TAKAHASHI Makoto OHNISHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.9, pp.1201-1207, 2013 (Released:2013-10-01)

参考文献数

47

被引用文献数

2 7

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Lyme disease Borrelia spp. are transmitted by Ixodes ticks, and more than 10 species of borreliae have been identified around the world. Recently, another Borrelia sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as Borrelia valaisiana-related sp. In the present study, we obtained and genetically characterized 19 B. valaisiana-related sp. strains from mammals and ticks. Genetic analyses showed that the Borrelia strains were distinct from B. valaisiana found in Europe. Multilocus sequence typing revealed that these Borrelia isolates formed a monophyletic group with B. yangtze strains in China. Some of the strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious in C3H/HeN mice. We observed that the Borrelia sp. was maintained in the Ixodes granulatus tick after molting. These results suggested that small mammals and I. granulatus were possible reservoir hosts and the vector tick for the Borrelia sp., respectively. B. valaisiana, originally found in Europe, was transmitted mainly by I. ricinus, and birds were mainly thought to be reservoir hosts. Our results suggested that Japanese isolates of B. yangtze (formerly B. valaisiana-related sp.) were distinguishable from B. valaisiana according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 into bioresource centers as a reference strain of B. yangtze(=DSM 24625, JCM 17189).

著者

Tatsuya HORI Ryuta YOSHIKUNI Masanori KOBAYASHI Eiichi KAWAKAMI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0303, (Released:2013-10-01)

被引用文献数

6

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The objective of the present study was to determine an optimum temperature and extender for short-term transport of canine ejaculated semen. There was no significant difference on the qualities of semen diluted two kinds of extender, egg yolk Tris-citrate fructose (EYT-FC) or glucose (EYT-GC) extender between the 2°C or 8°C or 12°C and 4°C control groups during storage for up to 48 hr, while the 16–24°C groups showed decreased sperm motility during storage for 48 hr. However, 2°C group showed slightly lower sperm motility and slightly higher sperm abnormality than 4°C group. Therefore, we concluded that semen qualities can be maintained for up to 48 hr when canine semen samples are extended with EYT-FC or EYT-GC and stored at the range of 4–12°C.

著者

Tae-Ho CHUNG Hee-Dong KIM Young-Sun LEE Cheol-Yong HWANG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0223, (Released:2013-09-20)

被引用文献数

5 14

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Helicobacter spp. may have multiple routes of transmission. It is unclear, however, whether the agent is zoonotic and therefore transmitted from an animal reservoir, including dogs. The aim of this population-based study was to assess the relationship between pet ownership or frequent exposure to dogs and Helicobacter spp. infection, especially focusing on HHLO-2 (Helicobacter heilmannii-like organisms type 2) in saliva and feces samples in Korea, using non-invasive genus/species-specific PCR. One hundred twenty-four eligible human subjects and 39 dogs participated in this study. Relativity of contact with dogs and Helicobacter spp. infection diagnosed by genus-specific PCR showed a statistically significant result (P<0.01), but in the relativity analyses between contact with dogs and H. pylori, H. felis and H. bizzozeronii infections diagnosed using species-specific PCR, only Helicobacter felis showed a statistically significant result. Although H. pylori infection showed a statistically significant relativity, no statistically significant association was found between veterinarian subjects and Helicobacter. spp., H. felis and H. bizzozeronii infections. On performing risk factor analyses of HHLO-2 infection by transmission, using matching species, between HHLO-2-positive dog owners and HHLO-2-positive dogs, Helicobacter felis infection showed an extremely significant relativity (P<0.0001) and Helicobacter bizzozeronii may also be a possible significant risk factor (P<0.01). These results suggest that HHLO-2 infection might be a zoonotic infection, because continuous contact with dogs was proved to be correlated with human H. felis and H. bizzozeronii infections in this study.

著者

Masashi WATANABE Kazuaki TANAKA Tatsuya TAKIZAWA Kazuhito SEGAWA Sakurako NEO Ryo TSUCHIYA Michiko MURATA Masaru MURAKAMI Masaharu HISASUE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0316, (Released:2013-09-13)

被引用文献数

1 2

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A polymorphic tetranucleotide (GAAT)n microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)5, (GAAT)6, (GAAT)7 and (GAAT)8, including 9 of the 10 expected genotypes. The expected heterozygosity (He) and the polymorphic information content (PIC) value of this microsatellite locus varied from 0.389 to 0.749 and from 0.333 to 0.682, respectively, among the 6 breeds. The allelic frequency differed greatly among breeds, but this microsatellite marker was highly polymorphic and could be a useful marker for the canine TNFA gene.

著者

Manabu KOIKE Yasutomo YUTOKU Aki KOIKE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0283, (Released:2013-09-10)

被引用文献数

4

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Various chemotherapeutic drugs, such as etoposide, and ionizing radiation (IR) have been clinically applied for the treatment of many types of animal and human malignancies. IR and chemotheraputic drugs kill tumor cells mainly by inducing DNA double-strand breaks (DSBs). On the other hand, unrepaired or incorrectly repaired DSBs can lead to chromosomal truncations and translocations, which can contribute to the development of cancer in humans and animals. Thus, it is important to clarify the molecular mechanisms underlying the chemosensitivity or radiosensitivity of mammalian cells in order to develop medical treatments and next-generation chemotherapeutic drugs for cancer. Previously, we established and analyzed cell lines stably expressing chimeric constructs of EGFP and the wild-type Ku80 (XRCC5) protein or its mutant protein to which mutations were introduced by the site-directed mutagenesis. We found that the Ku70 (XRCC6)-binding-site mutations (A453H/V454H) of Ku80 and nuclear localization signal (NLS)-dysfunctional mutations (K565A/K566A/K568A) affected the ability to complement etoposide sensitivity. In this study, we examined the radiosensitivity of these cell lines. We found that either or both amino acid substitutions in two functional domains of Ku80, i.e., Ku70-binding-site mutations (A453H/V454H) and NLS-dysfunctional mutations (K565A/K566A/K568A), affect the ability to complement radiosensitivity. Moreover, these mutations in the two domains of Ku80 affect the DSB-sensing ability of Ku80. These information and Ku80 mutant cell lines used might be useful for the study of not only the dynamics and function of Ku80, but also the molecular mechanism underlying the cellular response to IR and chemotherapeutic drugs in mammalian cells.