著者

松本 浩毅 山田 剛久 竹村 直行 左向 敏紀 小山 秀一 本好 茂一 稲田 頼太郎

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.58, no.8, pp.727-730, 1996-08-25

被引用文献数

1

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臨床的に健常でDirofilaria immitis (D.immitis)感染の認められない102頭のビーグルと16頭のD.immitis感染犬の血中免疫複合体(CIC)を免疫粘着赤血球凝集反応(IAHA)法によって測定した. 102頭の健常犬のCIC値は, 28.2±29.1μg/mlであった. CIC値に関しては, 年齢や性による有意差はみられなかった. D.immitis感染犬のCIC値(210±111.4μg/ml)は, 健常犬に比べ有意に高かった(P<0.01). 今回の実験結果より, IAHA法がイヌのCIC測定に適した方法であり免疫複合体介在性疾患の検出あるいは診断に応用可能であると考えられた.

著者

藤瀬 浩 比嘉 一成 中山 孝大 和田 香陽子 落合 秀治 田名部 雄一

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.59, no.6, pp.495-497, 1997-06-25

被引用文献数

4 31

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劣性である高K(HK)赤血球の表現型を持つ犬が, 日本犬の13の系統あるいは地方犬群の中の10群に発見された. HK犬の発生率は山陰柴犬, 信州柴犬および秋田犬で26-38%であり, その遺伝子頻度は0.513-0.612であった. 韓国の珍島犬は, HK犬の最も高い発生率を示し(42%), その遺伝子頻度は0.652であった. 韓国の他の2つの群もこの変異を保有していた. HK赤血球の表現型を持つ犬は, 台湾, インドネシア, モンゴル, ロシア(サハリン)の犬群には発見されなかったが, 日本および韓国に広がっていた.

著者

Azusa SOMEYA Ryoko FUKUSHIMA Michiko YOSHIDA Yasuyuki TANAHASHI Tangmunkhong PRAPEUK Reiko IIZUKA Hiroshi HIRAMI Atsushi MATSUDA Shunichi TAKAHASHI Goro KURITA Takashi KIMURA Misuzu SEO Masayuki FUNABA Yoshii NISHINO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0349, (Released:2014-05-08)

被引用文献数

3

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Borna disease virus (BDV) infection causes neurological disease in cats. Here, we report BDV infection in 199 hospitalized domestic cats in the Tokyo area. BDV infection was evaluated by detection of plasma antibodies against BDV-p24 or -p40. BDV-specific antibodies were detected in 54 cats (27.1%). Interestingly, the percentage of seropositive cats was not significantly different among the three clinical groups, i.e., healthy (29.8%), neurologically asymptomatic disease (22.2%) and neurological disease (33.3%). The specific antibodies were present even in cats aged below one year. The seropositive ratio was constant, irrespective of age and sampling season. The present study suggests that additional factors are required for onset of Borna disease in naturally infected cats and that BDV is transmitted through vertical routes in cats.

著者

Ryosuke KOBAYASHI Naoaki YAMADA Takashi KITAMORI Fumiyo KITAMORI Kazunari SATO Takuya DOI Yumi WAKO Junko SATO Minoru TSUCHITANI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0529, (Released:2014-05-08)

被引用文献数

2 2

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A dog developed a cervical mass, and computed tomography verified a mass surrounding the trachea with some pulmonary masses. Histopathologically, the cervical mass was composed of malignant neoplastic cells showing follicular appearance which reacted positive for thyroglobulin on immunohistochemistry. A characteristic feature of the tumor was abundant and metaplastic stromal components. Anastomosed collagenous tissues connecting to capsule of the tumor were abundant in the stroma. In parts of the collagenous tissues, mature cartilages and bones were continuously formed. There was no cellular atypia or invasion in the components. We diagnosed this case as follicular thyroid carcinoma with metaplastic stroma. This is the first case report that characterizes stromal components with chondroid and osseous metaplasia in a canine thyroid carcinoma.

著者

Hyung-Jin PARK Dong-Kyu SEO Kun-Ho SONG Kyoung-Won SEO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0015, (Released:2014-05-08)

被引用文献数

3 8

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A 9-month-old intact female Yorkshire terrier dog was presented with episodic partial seizure-like cramping of the limbs. The patient’s episodes began six months previously; the interval between episodes became shorter, and the duration of the episodes increased. Various tests including neurologic examination, blood examination, abdominal radiography, ultrasonographic examination, angiographic computed tomography (CT) and brain magnetic resonance imaging (MRI) detected no remarkable changes. After these tests were conducted, the patient’s condition was suspected to be canine epileptoid cramping syndrome (CECS), which could be a form of paroxysmal dyskinesia (PD), and as a trial therapy, Science Diet k/d (Hill’s Pet Nutrition, Topeka, KS, U.S.A.) was prescribed. The clinical signs were dramatically reduced after diet therapy, and we diagnosed the patient with CECS. This is the first case report of CECS in a Yorkshire terrier dog.

著者

Kazuyo TAIRA Toshimune NAGAI Takeshi OBI Kozo TAKASE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.76, no.4, pp.583-586, 2014 (Released:2014-05-01)

参考文献数

22

被引用文献数

5 51

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Broiler chicks were reared on either wet litter or dry litter to compare the development of footpad dermatitis (FPD). Broilers reared on wet litter first developed FPD at 14 days of age. Their FPD scores increased sharply after 21 days of age, reaching 2.92 at 42 days. In broilers reared on dry litter, FPD was first observed at 28 days of age, and the FPD score was only 0.70 at 42 days. When 21- or 28-day-old broilers that had been reared on wet litter and had developed FPD were moved to dry litter, the progression of FPD was suppressed or delayed. These results suggest that reducing litter moisture is effective in preventing FPD and suppressing disease progression.

著者

大橋 雅津代 佐藤 靖 岩田 博司 河合 光久 榑林 陽一

出版者

Japanese Society of Veterinary Science

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.69, no.12, pp.1223-1228, 2007-12-25

被引用文献数

4 23

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2、4、6-trinitrobenzene sulfonic acid(TNBS)をラットの近位大腸内に投与すると、遠位大腸の伸展刺激に対する痛み感受性の有意な増大が認められた。TNBSに直接暴露された近位大腸には粘膜壊死と炎症性細胞浸潤が認められ、組織中ミエロパーオキシダーゼの有意な増加が認められた。一方、遠位大腸には粘膜壊死は発生せず、ミエロパーオキシダーゼ含量の増加も認められなかったが、トルイジンブルー陽性粘膜型肥満細胞が有意に増加していた。さらに遠位大腸組織を器官培養し、粘膜型肥満細胞脱顆粒の特異的なマーカーであるrat mast cell protease-2(RMCP-2)の遊離量を測定したところ、TNBS処置ラットでは正常対象値と比較して有意なRMCP-2遊離量の増加が認められた。また、肥満細胞安定化作用を持つデドキサントラゾールの皮下投与によりTNBS誘発大腸過敏は有意かつ用量依存的に抑制された。以上の成績から、脱顆粒亢進を伴った大腸粘膜肥満細胞浸潤によるメディエーター遊離亢進がTNBSによる大腸過敏症の発現に関与していることが示唆された。

著者

宇仁 茂彦 松林 誠 池田 英一 鈴木 義孝

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.65, no.3, pp.s・v, 385-388, 2003-03-25

被引用文献数

3 10

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福井,滋賀,岐阜の3県において,捕獲されたニホンツキノワグマの各種内部臓器(肝臓,腎臓,心臓,肺,脾臓,リンパ節,皮膚)の組織学的検索を行った.その結果,18頭すべての肺の全葉の肺胞壁にHepatozoon sp.の未成熟および成熟メロントを見いだした.また,病理学的所見として,メロント,メロゾイトおよび虫体を包含する食細胞結節の周回に炎症性細胞潤滑は見られなかったが,崩壊過程を示す細胞,虫体および結節の周りには炎症性細胞浸潤を見いだした.クマにおけるヘパトゾーン症の最初の報告である.

著者

張 炯寛 小野 満 金 泰鍾 蔡 錦順 津嶋 良典 新倉 昌浩 見上 彪

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.58, no.11, pp.1057-1066, 1996-11-25

被引用文献数

6

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マレック病ウイルス(MDV)血清2型(MDV2)相同糖蛋白I(gI homolog)のORFは, 355アミノ酸残基をコードし得る1065塩基からなり, MDV血清1型(MDV1)と血清3型(七面鳥ヘルペスウイルス: HVT)とはアミノ酸配列において各々49%, 36%の相同性が認められた. また予測されるN-linkの糖鎖付加部位並びにシグナル配列や膜貫通部位が存在し, 膜糖蛋白の性状を有していた. 転写産物解析により, このORFの翻訳開始コードンの上流56-147 bpで転写される3.5 kb mRNAがMDV2 gI homologの特異転写産物として同定された. MDV2(HPRS24株)感染鶏由来の抗血清を用いた免疫沈降解析によりMDV2 gI homologを発現するリコンビナントバキュロウイルス(rAcMDV2gI)の蛋白発現を検討したところ, rAcMDV2gI感染Sf9細胞では45-43 kDaの特異バンドが検出された. またツニカマイシン処理により糖鎖付加阻止試験を行ったところ, MDV2gI homologの前駆体蛋白と見られる35 kDaのバンドが検出された. これらの発現蛋白はホモのみならずヘテロの血清型MDV(GA株, SB-1株, FC126株)感染鶏由来の抗血清によっても認識されたことから, 型間共通のエピトープの存在並びに3血清型によるMDV感染細胞でのgIの発現が示唆された.

著者

Yasuhiro YAMAUE Yoshinao Z. HOSAKA Masato UEHARA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0132, (Released:2014-04-30)

被引用文献数

1 7

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We aimed to document macroscopic variations in the cellular tapetum in the dog, to provide a histologic description of the macroscopic results and to evaluate the correlation between the macroscopic appearance and aging. Fifty three dogs including 5 beagles, 1 Chihuahua and 47 mixed breeds of each gender were used. For a macroscopic study, the fresh tapetal fundi were photographed using digital camera. For a histological study, the glutaraldehyde-formalin fixed eyes were embedded in nitrocellulose and stained with hematoxylin-eosin or thionine. The normal tapetum was triangular with the rounded angles and the smooth contour. The atypical tapetum was smaller and more variable in shape, contour and color than the normal one. In severe cases, the fundus was devoid of the tapetum. The atypical tapetum tended to increase in frequency with aging. Retinal pigment epithelial cells on the normal tapetum were unpigmented. In the eye with the atypical tapetum, regardless of tapetal size and shape, unpigmented retinal pigment epithelial cells showed a similar distribution to that on the normal tapetum, even in a dog without a tapetum. Although there is a congenitally hypoplastic tapetum, the atypical tapetum tends to increase in incidence and severity with aging.

著者

Mohsen HANIFEH Minna M RAJAMÄKI Laura MÄKITALO Pernilla SYRJÄ Satu SANKARI Susanne KILPINEN Thomas SPILLMANN

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0578, (Released:2014-04-18)

被引用文献数

1 5

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Matrix metalloproteinases (MMPs) 2 and 9 are zinc-dependent endopeptidases that contribute to the control of breakdown and reconstitution of extracellular matrix under both normal and pathological conditions. The main objective of this study was to identify the presence of MMP-2 and -9 in the mucosa of the small and large intestines of clinically healthy beagle dogs using gelatin zymography technique. Intestinal mucosa samples from four different parts of the intestine (duodenum, jejunum, ileum and colon) were taken from 12 healthy laboratory beagle dogs and examined histologically. Based on WSAVA histology standards, recorded findings of all samples were considered insignificant. Pro-MMP-2 and -9 activities were found in 17/48 (35%) and 25/48 (52%) of the samples, respectively. Among four different parts of the intestine of 12 dogs, the ileum had the highest positivity rates of 7/12 (58.3%) and 8/12 (66.7%) for pro-MMP-2 and -9 activities, respectively. However, statistical analysis showed no significant difference of pro-MMP-2 and -9 activities between the separate parts of the intestine (P>0.05). None of the intestinal samples showed gelatinolytic activity corresponding to the control bands of active MMP-2 and MMP-9. This study showed that pro-MMP-2 and -9 could be detected in the intestinal mucosa of healthy dogs using zymography, which seems to be a useful tool to evaluate the role of MMP-2 and -9 in the pathogenesis of canine chronic enteropathies, including inflammatory bowel diseases.

著者

Sunghoon JEON Seong Young KWON Rohani CENA Ju-hwan LEE Kyoungoh CHO Jung-Joon MIN Jihye CHOI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0627, (Released:2014-04-18)

被引用文献数

2

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A twelve-year-old, spayed female Yorkshire Terrier with intermittent vomiting was diagnosed with regional granulomatous enteritis through histopathological examination. On ultrasonography and computed tomography, a focal thickened duodenal wall showed a mass-like appearance with indistinct wall layers. Marked uptake of 18F-fluorodeoxyglucose was observed from the mass on positron emission tomography-computed tomography. Regional granulomatous enteritis is a rare form of inflammatory bowel disease and may have similar imaging features with intestinal tumors. This is the first study describing the diagnostic imaging features of ultrasonography, computed tomography and positron emission tomography-computed tomography of regional granulomatous enteritis in a dog.

著者

Adrian Patalinghug YBAÑEZ Rochelle Haidee D. YBAÑEZ Florencia G. CLAVERIA Mary Jane CRUZ-FLORES Xuen XUENAN Naoaki YOKOYAMA Hisashi INOKUMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0405, (Released:2014-04-09)

被引用文献数

4 23

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A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle.

著者

Naoki HIROSE Kazuyuki UCHIDA Hideyuki KANEMOTO Koichi OHNO James K. CHAMBERS Hiroyuki NAKAYAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0083, (Released:2014-04-09)

被引用文献数

1 42

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To determine the incidence of hepatic diseases in dogs and cats in Japan, a retrospective study was performed using data of 463 canine and 71 feline liver biopsies at the Veterinary Medical Center of the University of Tokyo. The most common canine hepatic disease was microvascular dysplasia (MVD) and occupied 29.4% of all diagnoses. This terminology might contain “real” MVD and primary portal vein hypoplasia, because these two conditions were difficult to be clearly distinguished histopathologically. Parenchymal and interstitial hepatitis and primary hepatic tumors accounted for 23.5% and 21.0% of the diagnoses, respectively. Parenchymal and interstitial hepatitis occupied 34.1% of non-proliferative canine hepatic diseases, while hepatocellular adenoma and carcinoma were 26.6% and 24.5% of proliferative hepatic diseases, respectively. Breed-specificity was seen in MVD for Yorkshire terrier, Papillon and Toy poodle, in hepatitis for Doberman pinscher and Labrador retriever, in cholangiohepatitis for American cocker spaniel, Miniature schnauzer and Pomeranian, in hepatocellular adenoma for Golden retriever and Shiba and in hepatocellular carcinoma for Shih Tzu. The most common feline liver disease was parenchymal and interstitial hepatitis (45.1% of all diagnoses). Among feline hepatitis, neutrophilic cholangiohepatitis (23.9%), lymphocytic cholangiohepatitis (14.1%) and chronic hepatitis (5.6%) were recorded. Adult polycystic liver disease was 5.6%. Among proliferative diseases in the feline liver (11.3% of the all), lymphoma (4.2%) and primary epithelial tumors (4.2%) including hepatocellular carcinoma, cholangiocellular adenoma and cholangiocellular carcinoma were observed. Hepatic degeneration was 14.1%, and MVD was 12.7%, respectively.

著者

筒井 茂樹 平沢 健介 武田 真記夫 板垣 慎一 河村 晴次 前田 健 見上 彪 土井 邦雄

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.59, no.9, pp.785-790, 1997-09-25

被引用文献数

7 20

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in vivoマウス肝細胞における高用量ガラクトサミン誘発アポトーシスについて検索した. 3g/kg GalN投与群では, Tdt-mediated dUTP nick end labeling (TUNEL) 陽性細胞が6時間後から観察されはじめ, HE染色切片における好酸性小体およびTUNEL陽性細胞の出現は, 24時間後で最も顕著になった. また, 48時間後では, 肝実質細胞の変性および壊死が目立ち, TUNEL陽性細胞はほとんど見られなくなった. 1.5g/kg GalN投与群では, 3g/kg GalN投与群に比べ, 病変は穏やかで, 好酸性小体およびTUNEL陽性細胞は24時間後ではほとんど見られず, 48時間後で顕著になった. さらに, アガロースゲル電気泳動によって見られるDNAの"はしご様"断片化パターンは, 3g/kg GalN投与後24時間および1.5g/kg GalN投与後48時間で最も顕著となったが, 3g/kg GalN投与後48時間では減少した. 一方, sGOTおよびsGPT活性は, 3g/kg GalN投与後48時間で著しく上昇した. これらの結果から, in vivoで高用量ガラクトサミンにより誘発される細胞死は, アポトーシスにより生じ, 後にネクローシスによる可能性が示唆された.

著者

Muneki HONNAMI Sungjin CHOI I-li LIU Wataru KAMIMURA Tetsushi TAGUCHI Hironori HOJO Nobuyuki SHIMOHATA Shinsuke OHBA Hiroyuki KOYAMA Ryohei NISHIMURA Ung-il CHUNG Nobuo SASAKI Manabu MOCHIZUKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0027, (Released:2014-03-26)

被引用文献数

1 5

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The effect of tetrapod-shaped alpha tricalcium phosphate granules (Tetrabones® [TB]) in combination with basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) on bone defect repair was examined. Bilateral segmental defects 20-mm long were created in the radius of 5 dogs, stabilized with a plate and screws, and implanted with 1 of the following: TB (TB group), TB and bFGF solution (TB/f group), and TB and f-IC gel (TB/f-IC group). Dogs were euthanized 4 weeks after surgery. Radiographs showed well-placed TB granules in the defects and equal osseous callus formation in all the groups. Histomorphometry revealed that the number of vessels and volume of new bone in the TB/f-IC group were significantly higher than those in the other groups. However, no significant differences in neovascularization and new bone formation were observed between the TB/f and TB groups. Furthermore, no significant difference in the lamellar bone volume or rate of mineral apposition was observed among groups. These results suggest that increased bone formation might have been because of the promotion of neovascularization by the f-IC gel. Therefore, the combinatorial method may provide a suitable scaffold for bone regeneration in large segmental long bone defects.

著者

長谷川 承 左向 敏紀 竹村 直行 小山 秀一 本好 茂一

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.54, no.4, pp.789-790, 1992-08-15

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陽イオン交換カラムを使用した高速液体クロマトグラフィー(HPLC)によりネコ糖化ヘモグロビンを測定した. 臨床上正常な猫37頭及び糖尿病猫12頭の糖化ヘモグロビンの百分比はHbAlcではそれぞれ1.70%および3.54%, HbAlでは1.88%および3.85%と, 糖尿病猫で有意な高値を示していた(Plt;0.05). このことからネコ糖化ヘモグロビンは糖尿病診断の指標になると思われた.

著者

Yohei SAKURAI Mika MISHINA Toshifumi WATANABE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0471, (Released:2014-03-20)

被引用文献数

2

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A 9-year-old female dog was referred to Azabu University Veterinary Teaching Hospital for dysuria and dyschezia after ovariohysterectomy. After a series of diagnostic imaging, vaginal obstruction was suspected by vaginal fluid retention. Surgical repair was attempted three times to establish patency through the vagina and the vestibule by episiotomy and laparotomy. Another laparotomy was performed to remove the entire vaginal mucosal layer to prevent recurrence, and which resulted in favorable outcome. Histopathological examination revealed that the resected tissue was a cyst originated from mesonephric duct remnant. In the present case, the cyst was curable by the entire resection of the cyst lining membrane, and which could eradicate all the secretory cells with least damage to the urethral vasculature and innervation.

著者

Hee-Chun LEE Ji-Hyun KIM Cho-Hee JEE Jae-Hoon LEE Jong-Hyun MOON Na-Hyun KIM Jung-Hyang SUR Kyu-Woan CHO Byeong-Teck KANG Jeongim HA Dong-In JUNG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0315, (Released:2014-03-20)

被引用文献数

1 8

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A 9-year-old castrated male Shih-tzu dog was referred to us because of chronic vomiting. The patient’s hematological, radiographic, ultrasonographic, endoscopic, and histological examinations were evaluated for diagnosis. Hematologic analysis indicated moderate anemia and azotemia. Based on imaging study, oval-shape mass was identified in gastric pylorus area. A proliferative mass was found on endoscopic examination and we performed biopsy using grasping forceps. The histopathological findings of the biopsy specimens indicated hypertrophic gastritis and Y-U pyloroplasty was performed. However, the histopathological examination of surgically resected mass was identified as tubular adenocarcinoma of stomach. Then carboplatin chemotherapy was performed 4 times for 13 weeks. The clinical sign such as vomiting were resolved gradually after surgery and chemotherapy, and the patient’s condition was managed favorably until recently (30 months after surgery). This case report demonstrated clinical features, imaging studies, endoscopic characteristics, histopathological and immunohistochemical features of gastric tubular adenocarcinoma as early gastric cancer in a dog.

著者

Toyotaka SATO Shin-ichi YOKOTA Risa ICHIHASHI Tomoka MIYAUCHI Torahiko OKUBO Masaru USUI Nobuhiro FUJII Yutaka TAMURA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0144, (Released:2014-03-18)

被引用文献数

7

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Understanding the prevalence of antimicrobial-resistance and the relationship between emergence of resistant bacteria and clinical treatment can facilitate design of effective treatment strategies. We here examined antimicrobial susceptibilities of Escherichia coli isolated from dogs admitted to a university hospital (University hospital) and companion animal clinics (Community clinics) in the same city, and investigated underlying multidrug-resistance mechanisms. The prevalence of E. coli with intermediate and resistant interpretations to ampicillin (AMP), enrofloxacin (ENR) and chloramphenicol (CHL) was higher in the University hospital than in the Community clinics cases. Use of antimicrobials, including fluoroquinolone, was also significantly higher in the University hospital than in the Community clinics cases. Upon isolation using ENR-supplemented agar plates, all ENR-resistant isolates had 3–4 nucleotide mutations that accompanied by amino acid substitutions in the quinolone-resistance-determining regions of gyrA, parC and parE, and 94.7% of all isolates derived from the University hospital showed AMP and/or CHL resistance, and possessed blaTEM and/or catA1. The average mRNA expression levels of acrA, acrB and tolC, and the prevalence of organic solvent tolerance, in isolates derived from ENR-supplemented agar plates were significantly higher in the University hospital than in the Community clinics isolates. Thus, E. coli derived from the University hospital cases more often showed concomitant decreased susceptibilities to aminopenicillins, fluoroquinolones, and CHL than did those derived from the Community clinics; this was related to an active AcrAB–TolC efflux pump, in addition to acquisition of specific resistance genes and genetic mutations.