1 0 0 0 OA 海外だより

出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.56, no.3, pp.191-192, 2016 (Released:2016-05-25)
著者
高橋 景一 真行寺 千佳子
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.27, no.4, pp.145-150, 1987-07-25 (Released:2009-05-25)
参考文献数
16

The sea urchin sperm flagella normally beat in a plane. The flagellar beat plane, however, rotates rapidly and reversibly when an appropriate external force is applied by imposing sinusoidal vibration of varying directions on the sperm head. The rotation may involve rotation of the central tubule complex within the flagellum.

1 0 0 0 OA 三重鎖DNA

著者
清水 光弘 神藤 平三郎 松本 潮
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.33, no.2, pp.68-73, 1993-03-25 (Released:2009-07-09)
参考文献数
44

Two types of DNA triplexes, intra-and intermolecular triplexes, at polypurine·polipyrimidine segments in both recombinant plasmids and DNA fragments were identified and characterized recently: the former forms in supercoiled plasmids, and the latter forms by combining duplex DNA and a single-stranded oligomer. These findings brought the research on the triplestranded DNA and RNA polymers discoverd 35 years ago into new phase. The possible biological significance of triplexes has been suggested by the fact that polypurine·polypyrimidine sequences are overrepresented in eukaryotic genomes and often are found near genes and recombinational hot-spots. The structural properties and biological implications of DNA triplexes are described.
著者
佐伯 泰
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.55, no.1, pp.019-022, 2015 (Released:2015-01-31)
参考文献数
16

The proteasome is a 2.5-MDa multisubunit protease complex that degrades polyubiquitylated proteins. Although its functions and structure have been extensively characterized, little is known about its molecular dynamics in living cells. By using fluorescence correlation spectrometry, a method for quantitative imaging, we determined the absolute concentration, dynamics, and complex formation of the proteasome in living cells. The proteasome is a highly mobile complex and almost all proteasome subunits are stably incorporated into the proteasomes. We also revealed that the proteasome completes its assembly process in the cytoplasm and translocates into the nucleus as a holoenzyme.