著者

Irene Ferreres Mirari Ortega Camilo López-Cristoffanini Salvador Nogués Xavier Serrat

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.36, no.4, pp.269-273, 2019-12-25 (Released:2019-12-27)

参考文献数

22

被引用文献数

5

---

Anther culture is a fast tool to obtain double haploid plant lines for breeding purposes. In rice, this procedure is commonly performed in two steps: i) induction of calli from anthers and ii) regeneration of plantlets from calli. It has been stated that genotype highly influences the anther culture efficiency, so the media used in each step should be optimized for each variety. In this study, we tested different media modifications of an efficient protocol optimized for a medium sized grain temperate japonica NRVC980385, used as a control, in a long grain temperate japonica rice variety (NRVC20120346), and two long grain tropical japonica varieties (303012 and 303013).We found that the addition of 150 mg l−1 colchicine to the induction medium worked best for all genotypes except for NRVC20120346, whose best induction was obtained with the colchicine-free medium. Referring to regeneration, increased gelling agent in the medium provided the best rates in NRVC980385, improving our former NRVC980385-optimized anther culture protocol. Sorbitol fortified regeneration medium worked the best in the case of the long grain varieties. The presence of colchicine in the induction medium was also related to a higher obtention of double haploid plantlets. This study highlights that genotype is a key factor in the performance of rice anther culture. It has set a first anther culture study on long grain japonica varieties and optimizes the anther culture protocol for temperate japonica medium grain NRVC980385 with the use of colchicine and other additives that increase osmotic stress.

著者

Shin-Ichiro KARAKI

出版者

Center for Academic Publications Japan

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.65, no.6, pp.498-506, 2019-12-31 (Released:2019-12-31)

参考文献数

42

被引用文献数

1

---

Diarrhea is the most common complication of enteral nutrition (EN). Pro/prebiotics are typically used to prevent diarrhea during EN. This study aimed to demonstrate the effects of enteral formula containing fermented dairy products (FDPs) and galacto-oligosaccharides on intestinal mucosal functions in rats. After feeding rats with regular rodent chow (RRC), standard formula (STD-F), and FDP-containing formula (FDP-F) for 2 wk, the rats were sacrificed with their intestines removed. Then, the electrophysiological properties of intestinal epithelia were measured using the Ussing chamber. In addition, organic acids and microbiota in the cecal contents were analyzed. In FDP-F-fed rats, electrical nerve activation-evoked increase in short-circuit current (Isc) in the cecum and middle colon was reduced compared with STD-F-fed rats. Mucosal propionate-evoked changes in Isc in FDP-F-fed rats were also reduced in the terminal ileum. The total cecal organic acid concentration in STD-F-fed rats decreased compared with RRC-fed rats, and approximately half was recovered in FDP-F-fed rats, which contributed to the recovery of acetate and butyrate concentrations. In microbiota analysis, the density of total bacteria, particularly Bifidobacterium, in cecal contents increased in FDP-F-fed rats. In conclusion, the consumption of FDP-F changed the total amounts and components of gut microbiota and organic acids, and resulted in inhibitory changes in mucosal luminal stimulant- and nervous system-mediated fluid secretory function. These findings suggest that FDP-F might prevent the incidence of diarrhea during EN.

著者

Noriko Fujihara Shin-ichi Yoshioka

出版者

Tottori University Medical Press

雑誌

Yonago Acta Medica (ISSN:13468049)

巻号頁・発行日

pp.2020.02.003, (Released:2019-12-23)

参考文献数

24

---

Background This study attempted to clarify issues regarding difficulties in school life perceived by professional training college students and educational support systems for students including possible developmental disabilities.Methods We surveyed 953 students enrolled at 9 professional training colleges in Japan by using an anonymous self-administered questionnaire to investigate difficulties during school life, help-seeking preferences, and self-esteem. Difficulties were investigated by using the Self-Cognitive Difficulties Scale, help-seeking preferences were assessed with the Help-Seeking Preferences Scale, and self-esteem was assessed by the Rosenberg Self-Esteem Scale. We also investigated the relationship between the Self-Cognitive Difficulties Scale and the sources of advice used by students.Results Responses were obtained from 863 students, and those of 775 students were considered to be valid. In terms of learning scenarios, 271 students (35.0%) responded that written examinations caused the most difficulties. The Rosenberg Self-Esteem Scale and Help-Seeking Preferences Scale were negatively correlated with the Self-Cognitive Difficulties Scale. With respect to the relationship between sub-factors of the Self-Cognitive Difficulties Scale and sources of advice, the students who asked specialists for advice had significantly higher scores for the factors of interpersonal relationships and reading/writing, as well as significantly higher scores for impulsivity and learning-related difficulties. The students who asked their previous high school teachers for advice had significantly higher scores for inattention and reading/writing. Furthermore, the students who asked senior students in the same department for advice had a significantly higher score for learning-related difficulties.Conclusion Our results suggested that professional training college students with a high Self-Cognitive Difficulties Scale score are more likely to choose a specialist as the source of advice. When providing educational support to professional training college students, it is important to consider the possibility that their sources of advice might differ depending on their individual self-perceived difficulty characteristics.

著者

Mercedes BECERRA-HERRERA Valentina MIRANDA Pablo RICHTER

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

pp.19P409, (Released:2019-12-27)

被引用文献数

6

著者

Celia Haig-Brown

出版者

Tillacum Library

巻号頁・発行日

1988

著者

Hiroyuki Kajiura Kyoko Hiwasa-Tanase Hiroshi Ezura Kazuhito Fujiyama

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.35, no.4, pp.375-379, 2018-12-25 (Released:2018-12-31)

参考文献数

17

被引用文献数

1 4

---

Miraculin is a promising protein with taste-modifying properties. Focusing on the unique function and potential of miraculin, recombinant miraculin production has been explored with the use of heterologous expression systems, but the activities of recombinant miraculins were much lower than those of native miraculin, probably due to the difference in post-translational modification, especially N-glycosylation. For practical use therefore, the differences between N-glycan of recombinant miraculin compared to that of native miraculin should be minimized. Here, to establish the platform for functional miraculin production, we expressed miraculin in tomato plants with the same taste-modifying activity as native miraculin purified from miracle fruit, and we compared the N-glycan structures with those of native miraculin. Our N-glycan structural analysis using purified miraculin, followed by hydrazynolysis, 2-pyridylamine (PA)-labeling, high-performance liquid chromatography, and a liquid chromatography tandem-mass spectrometry analysis revealed that both the native and recombinant miraculins carried an M3 structure as a predominant structure and that most of the N-glycan structures on the miraculins were pauci-mannosidic structures with a smaller amount of plant-specific α1,3-fucosylated and/or β1,2-xylosylated N-glycans and without a Lewis a epitope. These results indicate that the N-glycoform of native miraculin from miracle fruit and recombinant miraculin expressed in tomato plants are almost identical to each other with similar ratios and that, therefore, plant-specific N-glycans are essential for showing the full taste-modifying activity of miraculin.

著者

Thanida SANANMUANG Kanchanarat MANKONG Premsak JEERATANYASAKUL Kaj CHOKESHAI-USAHA Suppawiwat PONGLOWHAPAN

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0516, (Released:2019-12-27)

被引用文献数

2

---

A 3.5-year-old, 2.9 kg, multiparous Chihuahua presented with abdominal distension; pregnancy was diagnosed. On Day 7 before parturition, prenatal sonograms showed anechoic bilateral dilated cerebral lateral ventricles, suggesting fluid-filled regions (ventriculomegaly) in one foetus. A Caesarean section was performed and the male newborn had an abnormally enlarged dome-shaped head and a cleft lip, and died 6 days after birth. According to the family pedigree, the X-linked recessive inheritance of an orofacial cleft from the unaffected mother was suggested. This report clearly demonstrates that canine foetal ventriculomegaly (hydrocephalus) can be diagnosed in utero. For dog breeds predisposed to congenital ventriculomegaly, early detection is important for the prediction of perinatal survival and adequate supportive care can be applied at delivery.

著者

Ali Reza Fardin-Kia Sanjeewa R. Karunathilaka Betsy Jean Yakes Kyungeun Lee Zachary Ellsworth Lea Brückner Magdi M. Mossoba

出版者

Japan Oil Chemists' Society

雑誌

Journal of Oleo Science (ISSN:13458957)

巻号頁・発行日

vol.68, no.11, pp.1105-1112, 2019 (Released:2019-11-07)

参考文献数

29

被引用文献数

5

---

We recently observed that the weak near-infrared (NIR) band near 5260 cm–1 was relatively more intense for extra virgin olive oil (EVOO) than for refined olive oil (ROO). We also observed that its intensity was diminished upon heating and erroneously presumed that it may be attributed to volatile carbonyl components in EVOO. In the present study we demonstrate for the first time that this band is primarily attributed to a water O-H combination band. To accurately determine the intensity of this weak band, observed on a shifted and sloping baseline, we measured the peak-to-peak (p-p) height of its first derivative. An exponential calibration curve for p-p height versus gravimetrically-determined concentration of spiked water was satisfactorily generated. The calibration curve was first evaluated by using independent sets of gravimetrically prepared test samples. Subsequently, it was used to determine the moisture content, a quality parameter, for a limited set of authenticated reference olive oils whose quality and purity were confirmed by official methods. These concentrations, 0.098-0.12% H2O (w/w) for EVOO, 0.022-0.030% H2O (w/w) for ROO, and 0.028-0.054% H2O (w/w) for pomace olive oil (POO), were consistent with those reported in the literature. For 88 commercial products investigated, the moisture levels fell in the range from 0.026% to 0.13% (w/w). The correlation between moisture content and other olive oil quality parameters has been reported in the literature and has yet to be further investigated.

著者

Azuma Jun-ichi Sakamoto Masahiro

出版者

FCCA(Forum: Carbohydrates Coming of Age)

雑誌

Trends in glycoscience and glycotechnology (ISSN:09157352)

巻号頁・発行日

vol.15, no.81, pp.1-14, 2003-01-02

参考文献数

38

被引用文献数

1 56

---

直径が3nm以下のセルロースミクロフィブリルを含むハイドロコロイドがヤドリギ、<i>Viscum album</i> L.、の果実およびバジルの種子の外珠皮に存在している。ヤドリギの場合では、セルロースミクロフィブリルは viscin と呼ばれる組織中に存在するロープ状の細胞中に細胞の長軸に対して直角の方向にコイル状にパックされている。一方、バジルの場合では、種子の外珠皮に存在する筒形の細胞内にコイル状にパックされている。これらの細胞が含水するに伴ってセルロースミクロフィブリルはほどけて周囲に広がっていく。ハイドロコロイドは一種のセルロース-ヘミセルロース性の多糖のコンポジットである。両ハイドロコロイドには(1,4)-結合したキシランとグルコマンナンの他に高度に分岐したアラビノガラクタンが含まれていることがメチル化分析の結果わかった。ハイドロコロイドの部分酸加水分解によりグルコマンナンタイプのヘミセルロースがセルロースと密接な関係にあり、酸性の多糖が水不溶性のセルロースをコロイド状態に保つ役割をしていると考えられる。

著者

金 忠永 Kim Choong-Young

出版者

筑波大学比較・理論文学会

雑誌

文学研究論集 (ISSN:09158944)

巻号頁・発行日

no.19, pp.156-172, 2001-03-31

---

一、 はじめに 『風姿花伝』は世阿弥の能楽論書の中で前期の代表作であり、その中心課題は舞台上に「花」を咲かせることにある。この『風姿花伝』で説かれている「花」とは、能の魅力、あるいは能役者が観客に与える感動をさし、『風姿花伝』第七の別紙口伝には、 ...

著者

[a film by Marc-Henri Wajnberg]

出版者

ナウオンメディア (発売)

巻号頁・発行日

2003

著者

Hayato HARIMA Michihito SASAKI Masahiro KAJIHARA Akina MORI-KAJIHARA Bernard M. HANG’OMBE Katendi CHANGULA Yasuko ORBA Hirohito OGAWA Martin SIMUUNZA Reiko YOSHIDA Aaron MWEENE Ayato TAKADA Hirofumi SAWA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0424, (Released:2019-12-20)

被引用文献数

4

---

Orthoreoviruses have been indentified in several mammals, however, there is no information about orthoreoviruses in shrews. In this study, we screened wild animals in Zambia, including shrews, rodents, and bats for the detection of orthoreoviruses. Two orthoreovirus RNA genomes were detected from a shrew intestinal-contents (1/24) and a bat colon (1/96) sample by reverse-transcription (RT)-PCR targeting the RNA-dependent RNA polymerase gene of orthoreoviruses. Phylogenetic analyses revealed that each of the identified orthoreoviruses formed a distinct branch among members of the Orthoreovirus genus. This is the first report that shrews are susceptible to orthoreovirus infection. Our results suggest the existence of undiscovered orthoreoviruses in shrews and provide important information about the genetic diversity of orthoreoviruses.

著者

コリンヌ・ドゥベ-ヌ=フランクフォ-ル著 南條郁子訳

出版者

創元社

巻号頁・発行日

1999

著者

林 孟楠 LIN Meng-Nan

出版者

名古屋大学大学院法学研究科

雑誌

名古屋大學法政論集 (ISSN:04395905)

巻号頁・発行日

vol.262, pp.339-382, 2015-06-25

著者

Arata Yoshiaki Zhang Yue-Chang

出版者

高温学会

雑誌

高温学会誌 (ISSN:03871096)

巻号頁・発行日

vol.23, pp.1-56, 1997-01

被引用文献数

5

著者

田代 信 前島 弘則 戸田 謙一 R. Kelley L. Reichenthal J. Lobell R. Petre M. Guainazzi L. Angelini E. Costantini M. Edison 藤本 龍一 M. Grimm 林田 清 J-W. den Herder 石崎 欣尚 S. Paltani 松下 恭子 森 浩二 G. Sneiderman 竹井 洋 寺田 幸功 冨田 洋 XARM team

出版者

一般社団法人 日本物理学会

雑誌

日本物理学会講演概要集 73.1 (ISSN:21890803)

巻号頁・発行日

pp.494, 2018 (Released:2019-05-13)

著者

Israel Fernandez-Pineda David Marcilla Francisco Javier Downey-Carmona Sebastian Roldan Lucia Ortega-Laureano Jose Bernabeu-Wittel

出版者

The Editorial Committee of Annals of Vascular Diseases

雑誌

Annals of Vascular Diseases (ISSN:1881641X)

巻号頁・発行日

vol.7, no.3, pp.316-319, 2014 (Released:2014-09-25)

参考文献数

6

被引用文献数

7 30

---

The acronym FAVA (Fibro-Adipose Vascular Anomaly) has been recently given to a distinct vascular entity that is characterized by fibrofatty infiltration of muscle, unusual phlebectasia with pain, and contracture of the affected extremity. We report a new case of FAVA in a 10-year-old girl with pain in her right lower leg and equinus contracture. As in our case, FAVA typically presents in young females with calf involvement and limited ankle dorsiflexion with local pain. FAVA should be considered as a differential diagnosis when evaluating vascular anomalies in the lower extremities.

著者

Naoki SUGIMOTO Shu-ichi Nakano

出版者

公益社団法人 日本化学会

雑誌

化学と教育 (ISSN:03862151)

巻号頁・発行日

vol.51, no.7, pp.398-401, 2003-07-20 (Released:2017-07-11)

参考文献数

4

被引用文献数

1

---

人から人が産まれたり,子供が親に似るのは,形質が遺伝するためである。この遺伝という現象を化学の視点から捉えられるようになったのは,ここ50年のことである。この遺伝の本質を化学的に理解することは,病気の発症や予防などとの関連で注目を集めており,様々な生物に対して遺伝子の解明が進められている。ここでは,遺伝子の本体であるDNAの化学的な性質について説明し,DNAが遺伝物質である理由とその役割について解説する。

著者

Masaki MAEZAWA Ken-ichi WATANABE Noriyuki HORIUCHI Kotaro MATSUMOTO Yoshiyasu KOBAYASHI Hisashi INOKUMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0418, (Released:2019-12-04)

被引用文献数

12

---

We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) gene rearrangement, specifically with regard to its utility as a method to diagnose bovine B-cell lymphoma. PCR for IgH gene rearrangement indicated monoclonal proliferation of B-cells in 24 of 35 cattle with B-cell lymphoma. In contrast, PCR for IgH gene rearrangement in lymph nodes and tumor tissues from 65 cattle diagnosed with tumors other than B-cell lymphoma and non-tumors revealed polyclonal population of B-cells. Sensitivity, specificity, positive predictive value, and negative predictive value for PCR for IgH gene rearrangement for bovine B-cell lymphoma were 68.6%, 100%, 100%, and 85.5%, respectively. Clonality analysis using PCR for IgH gene rearrangement may be useful for adjunctive diagnosis of bovine B-cell lymphoma.

著者

Kensuke ORITO Asako KAWARAI-SHIMAMURA Atsushi OGAWA Atsushi NAKAMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.12, pp.1978-1982, 2017 (Released:2017-12-22)

参考文献数

22

被引用文献数

8

---

A prospective observational study was performed in canine clinical medicine to evaluate the emetic action and adverse effects of tranexamic acid. Veterinarians treated 137 dogs with a single dose of tranexamic acid (50 mg/kg, IV) after accidental ingestion of foreign substances. If needed, a second (median, 50 mg/kg; range, 20–50 mg/kg, IV) or third dose (median, 50 mg/kg; range, 25–50 mg/kg, IV) was administered. Tranexamic acid induced emesis in 116 of 137 (84.7%) dogs. Median time to onset of emesis was 116.5 sec (range, 26–370 sec), median duration of emesis was 151.5 sec (range, 30–780 sec), and median number of emesis episodes was 2 (range, 1–8). Second and third administrations of tranexamic acid induced emesis in 64.7 and 66.7% of dogs, respectively. In total, IV administration of tranexamic acid successfully induced emesis in 129 of 137 (94.2%) dogs. Adverse effects included a tonic-clonic convulsion and hemostatic disorder in two different dogs, both of which recovered after receiving medical care. Tranexamic acid induced emesis in most dogs following a single-dose. When a single dose was not sufficient, an additional dosage effectively induced emesis. Overall, adverse effects were considered low and self-limiting.