著者
Ho-Geun JEGAL Hyo-Jin PARK Jin-Woo KIM Seul-Gi YANG Min-Ji KIM Deog-Bon KOO
出版者
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
雑誌
Journal of Reproduction and Development (ISSN:09168818)
巻号頁・発行日
pp.2020-013, (Released:2020-04-22)
被引用文献数
5

Ruthenium red (RR) inhibits calcium (Ca2+) entry from the cytoplasm to the mitochondria, and is involved in maintenance of Ca2+ homeostasis in mammalian cells. Ca2+ homeostasis is very important for further embryonic development of fertilized oocytes. However, the effect of RR on mitochondria-Ca2+ (mito-Ca2+) levels during in vitro fertilization (IVF) on subsequent blastocyst developmental capacity in porcine is unclear. The present study explored the regulation of mito-Ca2+ levels using RR and/or histamine in fertilized oocytes and their influence on blastocyst developmental capacity in pigs. Red fluorescence intensity by the mito-Ca2+ detection dye Rhod-2 was significantly increased (P < 0.05) in zygotes 6 h after IVF compared to mature oocytes. Based on these results, we investigated the changes in mito-Ca2+ by RR (10 and 20 μM) in presumptive zygotes using Rhod-2 staining and mito-Ca2+ uptake 1 (MICU1) protein levels as an indicator of mito-Ca2+ uptake using western blot analysis. As expected, RR-treated zygotes displayed decreased protein levels of MICU1 and Rhod-2 red fluorescence intensity compared to non-treated zygotes 6 h after IVF. Blastocyst development rate of 20 μM RR-treated zygotes was significantly increased 6 h after IVF (P < 0.05) due to improved mitochondrial functions. Conversely, the blastocyst development rate was significantly decreased in histamine (mito-Ca2+ inhibitor, 100 nM) treated zygotes (P < 0.05). The collective results demonstrate that RR improves blastocyst development in porcine embryos by regulating mito-Ca2+ and MICU1 expression following IVF.
著者
Ping Ji Changmai Chen Yanan Hu Zixuan Zhan Wei Pan Rongrong Li Erguang Li Hui-Ming Ge Guang Yang
出版者
The Pharmaceutical Society of Japan
雑誌
Biological and Pharmaceutical Bulletin (ISSN:09186158)
巻号頁・発行日
vol.38, no.1, pp.1-6, 2015-01-01 (Released:2015-01-01)
参考文献数
21
被引用文献数
13 32

The bark, leaves, and flowers of Paulownia trees have been used in traditional Chinese medicine to treat infectious and inflammatory diseases. We investigated the antiviral effects of Paulownia tomentosa flowers, an herbal medicine used in some provinces of P. R. China for the treatment of skin rashes and blisters. Dried flowers of P. tomentosa were extracted with methanol and tested for antiviral activity against enterovirus 71 (EV71) and coxsackievirus A16 (CAV16), the predominant etiologic agents of hand, foot, and mouth disease in P. R. China. The extract inhibited EV71 infection, although no effect was detected against CAV16 infection. Bioactivity-guided fractionation was performed to identify apigenin as an active component of the flowers. The EC50 value for apigenin to block EV71 infection was 11.0 µM, with a selectivity index of approximately 9.3. Although it is a common dietary flavonoid, only apigenin, and not similar compounds like naringenin and quercetin, were active against EV71 infection. As an RNA virus, the genome of EV71 has an internal ribosome entry site that interacts with heterogeneous nuclear ribonucleoproteins (hnRNPs) and regulates viral translation. Cross-linking followed by immunoprecipitation and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that EV71 RNA was associated with hnRNPs A1 and A2. Apigenin treatment disrupted this association, indicating that apigenin suppressed EV71 replication through a novel mechanism by targeting the trans-acting factors. This study therefore validates the effects of Paulownia against EV71 infection. It also yielded mechanistic insights on apigenin as an active compound for the antiviral activity of P. tomentosa against EV71 infection.
著者
Shin-ya Ohba Yoshio Tsuda
出版者
The Japan Society of Medical Entomology and Zoology
雑誌
衛生動物 (ISSN:04247086)
巻号頁・発行日
vol.67, no.1, pp.45-50, 2016-03-25 (Released:2016-09-25)
参考文献数
29

The tsunami caused by the Great East Japan Earthquake on March 11, 2011 hit the Pacific coast and caused heavy destruction of natural and man-made environments in north-eastern Japan. This study focuses on mosquito larvae and their potential aquatic insect predators associated with ground pools and pools that appeared in the concrete foundations of destroyed houses (concrete pool) in inundated areas in Miyagi Prefecture, Japan. Field samplings were conducted on late July 2013. Culex inatomii, Cx. pipiens group, Cx. tritaeniorhynchus, and Cx. orientalis were collected from ground pools and concrete pools. The abundance of Cx. inatomii and Cx. pipiens groups in concrete pools was significantly greater than that in the ground pools. A large number of Hydroglyphus japonicus were collected as potential mosquito predators, followed by Micronecta spp., Enochrus japonicus, Rhantus sturalis, Aquarius paludm paludum and Hydrochara affinis, categorized as “flight dispersers,” which might immigrate rapidly from the non-inundated rice fields or wetlands. Stepwise generalized linear models suggested that larval abundance of Cx. inatomii in the pools studied was affected by the vegetation cover and habitat type (ground pool or concrete pool), but not by water depth, salinity, presence of predators, and bottom type (sand or concrete) of aquatic bodies. Concrete pools and covered with dense vegetation provide breeding habitat for Cx. inatomii along with their potential predators.
著者
FENG Z. -R. TAKAOKA TADAO
出版者
一般社団法人情報処理学会
雑誌
J. of Information Proc. (ISSN:03876101)
巻号頁・発行日
vol.10, pp.173-177, 1988
被引用文献数
1

It is shown how to modify the Boyer-Moore string matching algorithm so that the number of characters actually inspected and the running time decrease sharply as the length of pattern gets longer.
著者
Mei Ling Xu Ga Ram Wi Hyoung Jin Kim Hong-Jin Kim
出版者
The Pharmaceutical Society of Japan
雑誌
Biological and Pharmaceutical Bulletin (ISSN:09186158)
巻号頁・発行日
vol.39, no.4, pp.540-546, 2016-04-01 (Released:2016-04-01)
参考文献数
43
被引用文献数
4 19

Human respiratory syncytial virus (hRSV) is the most common cause of bronchiolitis and pneumonia in infants. The lack of proper prophylactics and therapeutics for controlling hRSV infection has been of great concern worldwide. Xylitol is a well-known sugar substitute and its effect against bacteria in the oral cavity is well known. However, little is known of its effect on viral infections. In this study, the effect of dietary xylitol on hRSV infection was investigated in a mouse model for the first time. Mice received xylitol for 14 d prior to virus challenge and for a further 3 d post challenge. Significantly larger reductions in lung virus titers were observed in the mice receiving xylitol than in the controls receiving phosphate-buffered saline (PBS). In addition, fewer CD3+ and CD3+CD8+ lymphocytes, whose numbers reflect inflammatory status, were recruited in the mice receiving xylitol. These results indicate that dietary xylitol can ameliorate hRSV infections and reduce inflammation-associated immune responses to hRSV infection.
著者
Masakazu Saito Satoru Watanabe Kaori Nimura-Matsune Hirofumi Yoshikawa Hitoshi Nakamoto
出版者
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
雑誌
The Journal of General and Applied Microbiology (ISSN:00221260)
巻号頁・発行日
pp.2020.02.001, (Released:2020-04-10)
参考文献数
29
被引用文献数
1

The CIRCE/HrcA system is highly conserved in cyanobacterial genomes. We have shown that heat-shock induction of the groESL1 operon in the cyanobacterium Synechocystis sp. PCC6803 is negatively regulated by the CIRCE/HrcA system. In Synechococcus elongatus PCC7942, a novel heat shock protein, Orf7.5, is involved in positive regulation of the groESL1 transcription. However, Orf7.5 is not conserved in some cyanobacteria, including Synechocystis sp. PCC6803. The purpose of this study is to evaluate the functional conservation of the CIRCE/HrcA system in S. elongatus PCC7942 and to understand the interplay between the CIRCE/HrcA system and the Orf7.5 regulatory system. We constructed single and double mutants of S. elongatus orf7.5, hrcA and orf7.5/hrcA and heat induction of the groESL1 transcription in these mutants was analyzed. Unexpectedly, derepression of the groESL1 transcription in an hrcA mutant was not observed. In all these mutants, the transcription was greatly suppressed under both normal and heat stress conditions, indicating that both HrcA and Orf7.5 are involved in regulation of the groESL1 transcription in a positive way. Consistent with the decrease in the groESL1 mRNA level, all the single and double mutants showed a great loss of acquired thermotolerance. Heat induction of the orf7.5 promoter activity was totally diminished in the orf7.5 mutant, indicating that Orf7.5 activates its own transcription. Yeast two hybrid analysis showed that the principle sigma factor RpoD1 interacts with Orf7.5. These results indicate that Orf7.5 enhances the transcription of groESL1 and orf7.5 by interacting with RpoD1.
著者
Haruka Takeuchi Chie Takahashi-Muto Mana Nagase Masahiro Kassai Rieko Tanaka-Yachi Chikako Kiyose
出版者
Japan Oil Chemists' Society
雑誌
Journal of Oleo Science (ISSN:13458957)
巻号頁・発行日
pp.ess19321, (Released:2020-04-13)
被引用文献数
23

Obesity, a lifestyle disease resulting from excessive caloric intake and insufficient physical activity, results in a state of chronic inflammation. A food ingredient that suppresses chronic inflammation could help prevent associated diseases. Sweet basil (Ocimum basilicum L.) is a herb from the Lamiaceae family with some reported anti-inflammatory effects. Via this in vitro study, we aimed to investigate whether sweet basil exerts anti-inflammatory effects in obese patients. Fresh sweet basil leaves were freeze-dried and powered. After that, this was extracted with 80% methanol. After 3T3-L1 adipocytes were cultured with sweet basil extracts at final concentrations of either 5 or 25 μg/mL for 24h, RAW264.7 macrophages were seeded onto this adipocytes and co-cultured for 12h. We determined the effects of sweet basil extracts on inflammatory cytokine expression by real-time PCR or western blotting. Sweet basil extracts reduced the expression of inflammatory cytokine mRNA induced by co-culture, including that of IL-6 (Il6), IL-1β (Il1b), TNF-α (Tnf), and CCL2 (Ccl2). In addition, sweet basil extracts suppressed the mRNA expression of NF-κB (Nfκb1), a transcription factor of inflammatory cytokines. In an investigation of costimulatory CD137 (Tnfrsf9)/CD137L inflammatory signaling, a member of the TNF super-family, sweet basil extracts inhibited Tnfrsf9 expression induced by the co-culture. Therefore, the results of this study indicated that sweet basil extracts have an anti-inflammatory effect against adipocyte-induced inflammation, possibly through suppression of Tnfrsf9 expression.
著者
Yumiko Imai Keiji Kuba Takayo Ohto-Nakanishi Josef M. Penninger
出版者
The Japanese Circulation Society
雑誌
Circulation Journal (ISSN:13469843)
巻号頁・発行日
pp.1002010600, (Released:2010-02-04)
参考文献数
66
被引用文献数
30 146

Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin-angiotensin system by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. For instance, in the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in SARS pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.
著者
伊藤 耕介 WU Chun-Chieh CHAN Kelvin T. F. TOUMI Ralf DAVIS Chris
出版者
公益社団法人 日本気象学会
雑誌
気象集誌. 第2輯 (ISSN:00261165)
巻号頁・発行日
vol.98, no.1, pp.5-17, 2020
被引用文献数
13

<p>台風の移動の基礎的な理解はかなり成熟しているが、注目に値する研究の進展が近年も見られる。本論文では、単純化された順圧モデル・精緻な物理モデル・データ解析によって、主に2014年以降に得られた台風の移動に関する新しい概念や既存の概念に関する新たな知見を集約する。これには、台風の移動に関する環境場と台風の相互作用、および、予測可能性の研究を含んでいる。指向流・βジャイア・非断熱加熱といった従来の概念は依然として重要であるが、台風の進路を説明するメカニズムをより正確に理解することは、さらなる進路予報の精度向上に向けて、重要な基礎をなすであろう。</p>
著者
Haruki Yamamoto Hiroko Kojima-Ando Kaori Ohki Yuichi Fujita
出版者
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
雑誌
The Journal of General and Applied Microbiology (ISSN:00221260)
巻号頁・発行日
pp.2020.01.009, (Released:2020-04-02)
参考文献数
62
被引用文献数
8

Protochlorophyllide (Pchlide) reduction is the penultimate step of chlorophyll (Chl) biosynthesis, and is catalyzed by two evolutionarily unrelated enzymes: dark-operative Pchlide oxidoreductase (DPOR) and light-dependent Pchlide oxidoreductase (LPOR). Because LPOR is the sole Pchlide reductase in angiosperms, dark-grown seedlings of angiosperms become etiolated. LPOR exists as a ternary complex of Pchlide-NADPH-LPOR to form paracrystalline prolamellar bodies (PLBs) in etioplasts. Because LPOR is distributed ubiquitously across oxygenic phototrophs including cyanobacteria, it would be important to determine whether cyanobacterial LPOR has the ability to form PLBs. We isolated a DPOR-less transformant ΔchlL/LPORox, carrying a plasmid to overexpress cyanobacterial LPOR in the cyanobacterium Leptolyngbya boryana. The transformant did not produce Chl in the dark and became etiolated with an accumulation of Pchlide and LPOR. Novel PLB-like ultrastructures were observed in etiolated cells, which disappeared during the early stage of the light-dependent greening process. However, the rate of Chl production in the greening process of ΔchlL/LPORox was almost the same as that observed in the control cells, which carried an empty vector. An in vitro LPOR assay of extracts of dark-grown ΔchlL/LPORox cells suggested that the PLB-like structures are deficient in NADPH. Low-temperature fluorescence emission spectra of membrane fractions of the etiolated cells indicated the absence of the photoactive form of Pchlide, which was consistent with the inefficiency of the greening process. Cyanobacterial LPOR exhibited an intrinsic ability to form PLB-like ultrastructures in the presence of the co-accumulation of Pchlide; however, the PLB-like structure differed from the authentic PLB regarding NADPH deficiency.
著者
Jiahua Yu Qian-Ping Gu
出版者
IJNC Editorial Committee
雑誌
International Journal of Networking and Computing (ISSN:21852839)
巻号頁・発行日
vol.6, no.2, pp.149-166, 2016 (Released:2017-03-23)
参考文献数
28

Embedding graphs on the torus is a problem with both theoretical and practical importance. It is required to embed a graph on the torus for solving many application problems such as VLSI design, graph drawing and so on. Polynomial time and exponential time algorithms for embedding graphs on the torus are known. However, the polynomial time algorithms are very complex and their implementation has been a challenge for a long time. On the other hand, the implementations of some exponential time algorithms are known but they are not efficient for large graphs in practice. To develop an efficient practical tool for embedding graphs on the torus, we propose a new exponential time algorithm for embedding graphs on the torus. Compared with a well used previous exponential time algorithm, our algorithm has a better practical running time.
著者
Xing-Bing Pan Hui-Jun Wang Bing Zhang Ying-Li Liu Su-Fen Qi Qing-Bao Tian
出版者
Japan Epidemiological Association
雑誌
Journal of Epidemiology (ISSN:09175040)
巻号頁・発行日
vol.30, no.3, pp.128-135, 2020-03-05 (Released:2020-03-05)
参考文献数
32
被引用文献数
8

Background: The prevalence of overweight is increasing dramatically worldwide. The aim of our study was to investigate the association of plain water intake (PWI) with the risk of new-onset overweight risk among Chinese adults.Methods: A total of 3,200 adults aged 18–65 who were free of overweight at baseline were enrolled from China Health and Nutrition Survey (CHNS) cohort study in 2006–2011. The risk of new-onset overweight with different amounts of PWI per day was analyzed in this 5-year cohort. A multiple logistic regression model was used to assess the association of PWI and the risk of new-onset overweight and adjust for potential confounders. Moreover, dose-response models were developed to estimate the linear relationship.Results: During 5 years of follow-up, 1,018 incident cases were identified. Our analysis indicated an inverse association of more than 4 cups of PWI per day and the risk of new-onset overweight among normal weight individuals. Compared with participants who drank 2 to 3 cups PWI, the adjusted odds ratios (OR) of overweight were 0.741 (95% confidence interval [CI], 0.599–0.916) in participants who drank 4 to 5 cups PWI, and 0.547 (95% CI, 0.435–0.687) in participants who drank more than 6 cups PWI. The dose-response analysis showed that every cup of PWI was associated with a 6.5% and 8.4% decrease in the risk of new-onset overweight among men and women, respectively. The interactions of PWI and covariates on the risk of overweight were not found.Conclusion: Drinking more than 4 cups (≈1 liter) per day of plain water is associated with decrease in the risk of new-onset overweight among normal-weight individuals.
著者
Keiko Makita-Suzuki Chihaya Kakinuma Akira Inomata Yasuhiro Shimada Takefumi Hara Takashi Yao
出版者
JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY
雑誌
Journal of Toxicologic Pathology (ISSN:09149198)
巻号頁・発行日
vol.33, no.1, pp.1-9, 2020 (Released:2020-01-24)
参考文献数
40
被引用文献数
2

Although several liposomal drugs, including liposomal doxorubicin, have been approved, the etiology of the pathological responses caused by their physicochemical properties remains unknown. Herein, we investigated the pathological changes in the liver and the gallbladder of dogs following a single injection of liposomal doxorubicin (1 or 2.5 mg/kg) or an empty liposomal formulation (i.e., liposomal formulation without doxorubicin, ca. 21 mg/kg as lipid content). Injection of liposomal doxorubicin or the empty liposomal formulation induced hemorrhagic changes in the liver and the gallbladder. These changes were accompanied by minimal cellular infiltration with no obvious changes in the blood vessels. As there were no differences in the incidence and severity of hemorrhage between the groups administered comparable amounts of total lipid, the physicochemical properties of the liposomal formulation rather than an active pharmacological ingredient, doxorubicin, were associated with the hemorrhagic changes. Furthermore, decreased cytoplasmic granules with low electron density in mast cells beneath the endothelium of the hepatic vein were observed in the liver of dogs treated with liposomal doxorubicin or empty liposomal formulation. Injection of compound 48/80, a histamine releaser induced comparable hemorrhage in dogs, implying that hemorrhage caused by injection of liposomal doxorubicin or the empty liposomal formulation could be attributed to the histamine released from mast cells. The absence of similar hemorrhagic lesions in other species commonly used in toxicology studies (i.e., rats and monkeys), as well as humans, is due to the lack of mast cells beneath the endothelium of the hepatic vein in these species.
著者
Miura Akira Rosero-Navarro Nataly Carolina Sakuda Atsushi Tadanaga Kiyoharu Phuc Nguyen H. H. Matsuda Atsunori Machida Nobuya Hayashi Akitoshi Tatsumisago Masahiro
出版者
Nature Publishing Group
雑誌
Nature Reviews Chemistry (ISSN:23973358)
巻号頁・発行日
vol.3, no.3, pp.189-198, 2019-02-19
被引用文献数
245

Solid sulfide electrolytes are key materials in all-solid-state lithium batteries because of their high lithium-ion conductivity and deformability, which enable the lithium-ion path to be connected between the material’s grain boundaries under pressure near room temperature. However, sulfur species are moisture-sensitive and exhibit high vapour pressures; therefore, syntheses of sulfide electrolytes need to be carefully designed. Liquid-phase reactions can be performed at low temperatures in controlled atmospheres, opening up the prospect of scalable processes for the preparation of sulfide electrolytes. Here, we review liquid-phase syntheses for the preparation of sulfide-based solid electrolytes and composites of electrolytes and electrodes, and we compare the charge–discharge performances of the all-solid-state lithium batteries using these components.
著者
Myoung Soo KIM Jong Joo RHEE Seung Jun LEE Seon-Joo KWON Chae Heuck LEE
出版者
The Japan Neurosurgical Society
雑誌
Neurologia medico-chirurgica (ISSN:04708105)
巻号頁・発行日
vol.47, no.9, pp.419-423, 2007 (Released:2007-09-25)
参考文献数
25
被引用文献数
2 4

An 11-year-old girl with obstructive hydrocephalus developed akinetic mutism after treatment for hydrocephalus due to aqueductal stenosis by ventriculoperitoneal (VP) shunting. Bilateral chronic subdural hematomas developed about 2 months after insertion of the VP shunt and were evacuated. Postoperatively, the patient developed akinetic mutism, but her condition improved after administration of bromocriptine. Absence of abnormalities on dopamine transporter single photon emission computed tomography, lack of clinical response to levodopa treatment, and normal homovanillic acid concentration in the cerebrospinal fluid all indicated normal dopamine production. Pressure on the periventricular monoamine projections in the thalamus and hypothalamus without major dopamine deprivation in the striatum may have been the most important factors in the development of akinetic mutism in this patient.
著者
Yoshiki Imamura Akiko Okada-Ogawa Noboru Noma Takahiro Shinozaki Kosuke Watanabe Ryutaro Kohashi Masamichi Shinoda Akihiko Wada Osamu Abe Koichi Iwata
出版者
Nihon University School of Dentistry
雑誌
Journal of Oral Science (ISSN:13434934)
巻号頁・発行日
pp.19-0459, (Released:2020-03-11)
参考文献数
87
被引用文献数
7

Burning mouth syndrome (BMS) is one of the most frequently seen idiopathic pain conditions in a dental setting. Peri- and postmenopausal women are most frequently affected, and patients who experience BMS complain of persistent burning pain mainly at the tip and the bilateral border of the tongue. Recent studies have assessed whether BMS is a neuropathic pain condition, based on morphologic changes in biopsied tongue specimens, and whether there are abnormal pain responses in patients with this disease. Somatosensory studies have reported some abnormal findings in sensory and pain detection thresholds with inconsistency; however, the most distinct finding was exaggerated responses to painful stimuli. Imaging and electrophysiologic studies have suggested the possibility of dysregulation of the pain-modulating system in the central nervous system, which may explain the enhanced pain responses despite the lack of typical responses toward quantitative sensory tests. Basic studies have suggested the possible involvement of neuroprotective steroids, although the underlying mechanisms of this condition have not been elucidated. Experimental studies are looking for preferable supportive therapies for BMS patients despite the obscure pathogenesis.
著者
YOKOTA Shun-ichi
出版者
東京大学大学院農学生命科学研究科附属演習林
雑誌
東京大学農学部演習林報告 (ISSN:03716007)
巻号頁・発行日
vol.52, pp.165-171, 1956

It is natural that butt rot fungi should invade healthy trees through scars on the trunk. Besides, one of the modes of infection and propagation of butt rot was discovered by dissecting in detail decayed butts and roots of Sakhalin fir (Abies sachalinensis MAST.) grown in the natural forest in the Tokyo University Forest, Hokkaido.
著者
Echenique-Díaz Lázaro M. OHDACHI Satoshi KITA Masaki Begué-Quiala Gerardo Páez Rafael Borroto Labañino Jorge L. Delgado Díez Jorgelino Gámez HOSON Osamu SAITO Chiemi
出版者
宮城教育大学環境教育実践研究センター
雑誌
環境教育研究紀要 (ISSN:13448005)
巻号頁・発行日
vol.16, pp.89-95, 2014-03

Assessing local people's knowledge of endangered species is important not only for the planning and implementation of environmental education programs, but also to address community-based conservation issues and guarantee an effective engagement of local residents in conservation efforts. A questionnaire-based study conducted in Alejandro de Humboldt National Park assessed local people’s knowledge of the endangered Cuban Solenodon (Solenodon cubanus). From this it was found that although children seem to be better informed than adults with respect to the Cuban Solenodon, more needs to be done to educate both age and groups alike. This study also highlighted local residents’ positive disposition to actively participate in the Cuban Solenodon conservation effort.