著者

井上 敏 義永 忠一 野尻 亘 Satoshi Inoue Tadakazu Yoshinaga Wataru Nojiri

雑誌

桃山学院大学総合研究所紀要 = ST.ANDREW'S UNIVERSITY BULLETIN OF THE RESEARCH INSTITUTE (ISSN:1346048X)

巻号頁・発行日

vol.30, no.1, pp.21-42, 2004-07-01

---

Sensyu District is in the southern part of Osaka Prefecture. In this region, since the 18th century, many farmers had cultivated cotton and spun cotton into thread. However, because of the importation of cotton to Japan in the latter half of the 19th century, farmers had abandoned cultivation of cotton. On the other hand, the tradition of spinning cotton as a side job for farmers led the development of the thriving textile industries in this region. Up to World War II cotton textiles had been the most important export for Japan since the 19th century. Osaka City was the center of exporting cotton textiles. The location adjacent to Osaka City was advantageous for textile industries. Miscellaneous textile products, for example, cotton textiles, blankets, towels, knitwear, carpets etc. are manufactured by medium and small sized factories in Sensyu District. The networks of these companies and related industries have formed the agglomeration of textile industries in this region. The indigenous labor market is composed of specialized workers for textile industries. The trade unions and public institutions are engaged in quality control and development of new products for textile industries. Today, the incursion of cheap imports of textiles from Asia, the lack of successors in the workforce and the stagnation of productivity have gradually brought about the decline of textile industries in Sensyu District.

著者

Keiko MORITO Toshiharu HIROSE Junei KINJO Tomoki HIRAKAWA Masafumi OKAWA Toshihiro NOHARA Sumito OGAWA Satoshi INOUE Masami MURAMATSU Yukito MASAMUNE

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.24, no.4, pp.351-356, 2001 (Released:2002-04-26)

参考文献数

37

被引用文献数

391 471

---

The human estrogen receptor (hER) exists as two subtypes, hER α and hER β, that differ in the C-terminal ligand-binding domain and in the N-terminal transactivation domain. In this study, we investigated the estrogenic activities of soy isoflavones after digestion with enteric bacteria in competition binding assays with hER α or hER β protein, and in a gene expression assay using a yeast system. The estrogenic activities of these isoflavones were also investigated by the growth of MCF-7 breast cancer cells. Isoflavone glycoside binds weakly to both receptors and estrogen receptor-dependent transcriptional expression is poor. The aglycones bind more strongly to hER β than to hER α. The binding affinities of genistein, dihydrogenistein and equol are comparable to the binding affinity of 17 β-estradiol. Equol induces transcription most strongly with hER α and hER β. The concentration required for maximal gene expression is much higher than expected from the binding affinities of the compounds, and the maximal activity induced by these compounds is about half the activity of 17 β-estradiol. Although genistin binds more weakly to the receptors and induces transcription less than does genistein, it stimulates the growth of MCF-7 cells more strongly than does genistein.

著者

Hassadin BOONSRIROJ Daria Llenaresas MANALO Kazunori KIMITSUKI Taichi SIHMATSU Nozomi SHIWA Harumi SHINOZAKI Yurika TAKAHASHI Naoto TANAKA Satoshi INOUE Chun-Ho PARK

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0308, (Released:2015-08-14)

被引用文献数

3 13

---

Rabies is a zoonotic disease caused by the rabies virus. While the salivary glands are important as exit and propagation sites for the rabies virus, the mechanisms of rabies excretion remain unclear. Here, we investigated the histopathology of the salivary glands of rabid dogs and analyzed the mechanism of excretion into the oral cavity. Mandibular and parotid glands of 22 rabid dogs and three control dogs were used. Mild to moderate non-suppurative sialadenitis was observed in the mandibular glands of 19 of the 22 dogs, characterized by loss of acinar epithelium and infiltration by lymphoplasmacytic cells. Viral antigens were detected in the mucous acinar epithelium, ganglion neurons and myoepithelium. Acinar epithelium and lymphocytes were positive for anti-caspase-3 antibodies and TUNEL staining. In contrast, no notable findings were observed in the ductal epithelial cells and serous demilune. In the parotid gland, the acinar cells, myoepithelium and ductal epithelium all tested negative. These findings confirmed the path through which the rabies virus descends along the facial nerve after proliferation in the brain to reach the ganglion neurons of the mandibular gland, subsequently traveling to the acinar epithelium via the salivary gland myoepithelium. Furthermore, the observation that nerve endings passing through the myoepithelium were absent from the ductal system suggested that viral proliferation and cytotoxicity could not occur there, ensuring that secretions containing the virus are efficiently excreted into the oral cavity.

著者

井上 敏 Satoshi Inoue 桃山学院大学経営学部

出版者

桃山学院大学総合研究所

雑誌

桃山学院大学総合研究所紀要 = ST.ANDREW'S UNIVERSITY BULLETIN OF THE RESEARCH INSTITUTE (ISSN:1346048X)

巻号頁・発行日

vol.34, no.3, pp.85-95, 2009-03-18

---

Present Japan is confronted with a grave economic crisis. Museums are being forced to streamline their management under pressure from the economic situation affecting Japanese society overall. But the legal system for museums in Japan is not prepared for optimal management of museums. What is needed is an open discussion on implementing a new system under the concept of "intellectual freedom" in museums. This paper will examine this concept by looking at two key themes. One of these is Dr. Juzo Arai's theory of museum studies. The other is the legal case of the Museum of Modern Art, Toyama. In his theses, Dr. Arai emphasized the importance of selfgoverned organization by gakugeiin ( Japanese Curator) of local museums. This suggestion was indeed used as factual evidence in the legal case of the Museum of Modern Art, Toyama (1986). In Japan's present museum system, little attention is given to the opinions of gakugeiin, creating the need to organize independently from local governments. The concept of "intellectual freedom" in museums must be discussed as a first step toward revising the Museum Act in Japan.

著者

Daisuke KOJIMA Chun-Ho PARK Yusuke SATOH Satoshi INOUE Akira NOGUCHI Toshifumi OYAMAD

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.71, no.3, pp.319-324, 2009 (Released:2009-04-04)

参考文献数

31

被引用文献数

20 27

---

Fixed rabies viruses (CVS-11 strain) were inoculated intramuscularly to C57BL/6J mice, and the pathomorphological changes of the spinal cord including dorsal root spinal ganglion cells were investigated. At 4 days postinoculation (PI), viral antigens were first detected in the spinal neurons and dorsal root spinal ganglion cells without producing morphological changes. At 5 days PI, mild infiltration of lymphocytes was observed around the central canal, small blood vessels and leptomeninges. Cells positive to anti-Iba1 and anti-GFAP antibodies increased significantly from 3 to 5 days PI, respectively. Microglia changed their morphological forms to be ramified or amoeboid, and astroglia extended their cytoplasm from the leptomeninges to the parenchyma. At 7 days PI, apoptotic cells were found in the spinal cord and dorsal root spinal ganglion using TUNEL. We confirmed that most of T lymphocytes and a minority of microglial cells underwent apoptosis, using a combination of TUNEL and immunostaining with antibodies to viral phosphoprotein, CD3, Iba1 and GFAP. On the other hand, astroglial cells and virus-infected nerve cells were negative against TUNEL and cleaved caspase-3 antibody. These findings indicate that T lymphocytes and microglial cells died by apoptosis, whereas virus-infected nerve cells died by necrosis. This was accompanied by increased numbers and morphological changes of glial cells associated with the pathogenesis of CVS-11 in the C57BL/6J mouse.

著者

Nanase Kubo Chun-ho Park Satoshi Inoue Hajime Hatta

出版者

Japan Poultry Science Association

雑誌

The Journal of Poultry Science (ISSN:13467395)

巻号頁・発行日

vol.60, no.2, pp.2023014, 2023 (Released:2023-06-17)

参考文献数

26

---

Immunization of egg-laying hens with viral antigens efficiently produces large amounts of virus-specific IgY antibodies from egg yolks. A supply of practical and economical antibodies against the rabies virus is being desired worldwide. We immunized hens with the antigen gene DNA of the rabies virus, purified specific IgY antibodies from the egg yolk, and characterized the immuno-protein chemistry for use as a diagnosis. To prepare specific IgY antibodies against rabies virus nucleoprotein (RV-N) by DNA immunization, laying hens were pre-injected with λ-carrageenan or Freund’s complete adjuvant to increase local immune activity (pre-immune stimulation), and then immunized with RV-N recombinant plasmid DNA. RV-N-specific IgY antibodies were prepared from egg yolks of immunized hens. For comparison, conventional protein antigen immunization was also used to induce the production of RV-N-specific IgY antibodies. Laying hens were immunized with an RV-N protein antigen and RV-N-specific IgY was purified from egg yolks. The binding activity against RV-N antigens was examined using IgY samples prepared by DNA (with pre-immune stimulation) and protein immunization. Immunohistochemical staining showed that IgY antibodies prepared by protein immunization strongly detected viral antigens in the brain sections of dogs infected with the virus, whereas IgY antibodies prepared by DNA immunization did not. Enzyme-linked immunosorbent assay was performed using a commercially available rabies vaccine (inactivated virus) treated with 10% formalin and heating (60°C, 30 min and 90°C, 5 min). IgY prepared by DNA immunization had weaker reactivity with denatured antigens and lower antigen concentrations than IgY prepared by protein immunization. These results suggest that it is necessary to develop a DNA immunization method for inducing IgY antibodies against the rabies virus that strongly bind to native and denatured antigens to prepare specific IgYs that can be used for antigen detection in clinical tests.

著者

田村 達 桒田 但馬 井上 都之 佐藤 公子 Toru Tamura Tajima Kuwada Satoshi Inoue Kimiko Sato

出版者

岩手県立大学社会福祉学部

雑誌

岩手県立大学社会福祉学部紀要 = Bulletin of the Faculty of Social Welfare, Iwate Prefectural University (ISSN:13448528)

巻号頁・発行日

vol.25, pp.37-46, 2023-03-24

著者

Dai Kamamoto Shoko Takahashi Satoshi Inoue Masateru Katayama Sadao Suga

出版者

The Japanese Society for Neuroendovascular Therapy

雑誌

Journal of Neuroendovascular Therapy (ISSN:18824072)

巻号頁・発行日

pp.tn.2022-0005, (Released:2022-06-18)

参考文献数

11

被引用文献数

1

---

Objective: The Mo.Ma Ultra is an embolic protection device used in carotid artery stenting (CAS). In cases of left internal carotid artery stenosis (ICS) in which the common carotid artery (CCA) branches off the aortic arch at a steep angle, insertion of the Mo.Ma Ultra into the CCA is sometimes difficult. We introduce a “buddy catheter technique” that helps guide the Mo.Ma Ultra into the CCA, with an additional 4 Fr catheter into the external carotid artery.Case Presentation: An 84-year-old man with left ICS whose CCA also branched off the aortic arch at a steep angle also underwent CAS. The “buddy catheter technique” was used, and the Mo.Ma Ultra was inserted smoothly. The buddy catheter technique displaces the left CCA upward. Displacement straightens the vessels anatomically, and the ledge effect can be prevented by aligning the course of the vessels with the wire. Nevertheless, this technique requires bilateral femoral puncture, and so, complications can occur.Conclusion: The buddy catheter technique may be considered in cases in which the left CCA branches off the aortic arch at a steep angle.

著者

Chun-Ho PARK Sayaka KUBONIWA Ryo MURAKAMI Nozomi SHIWA Satoshi INOUE Kazunori KIMITSUKI Ma. Ricci R. GOMEZ Mark Joseph M. ESPINO Alpha Grace B. CABIC Sheila Marie C. ESPOSO Daria L. MANALO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.83, no.10, pp.1563-1569, 2021 (Released:2021-10-05)

参考文献数

27

被引用文献数

2

---

The rabies virus is one of the most neurotropic of all viruses infecting mammals. During the terminal phases of infection, the virus spreads to peripheral tissues, including the skin. The external skin of the nose, called the nasal planum, is a sensory organ where numerous nerve bundles and terminal nerves are distributed. Therefore, the nasal planum is expected to serve as a postmortem diagnostic material. However, the distribution of rabies virus antigens in the nasal planum in rabid animals has not yet been studied. In this study, the nasal planum was obtained from 45 rabid dogs. In all rabid dogs, the viral antigen was detected in the peripheral nerve tissues, Merkel cells, and squamous cells. The viral antigen in the epidermis exhibited three patterns: first, a diffuse positive pattern from the basal layer to the squamous layer; second, a reticular positive pattern along the cell membrane in the squamous layer; and third, a basal layer pattern of the epidermis. In the dermis, viral antigens were detected more often in lamellated corpuscles just beneath the rete pegs. These results suggest that the nasal planum could serve as a useful alternative source for postmortem diagnosis in rabies endemic countries.

著者

Chun-Ho PARK Sayaka KUBONIWA Ryo MURAKAMI Nozomi SHIWA Satoshi INOUE Kazunori KIMITSUKI Ma. Ricci R. GOMEZ Mark Joseph M. ESPINO Alpha Grace B. CABIC Sheila Marie C. ESPOSO Daria L. MANALO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0438, (Released:2021-09-02)

被引用文献数

2

---

The rabies virus is one of the most neurotropic of all viruses infecting mammals. During the terminal phases of infection, the virus spreads to peripheral tissues, including the skin. The external skin of the nose, called the nasal planum, is a sensory organ where numerous nerve bundles and terminal nerves are distributed. Therefore, the nasal planum is expected to serve as a postmortem diagnostic material. However, the distribution of rabies virus antigens in the nasal planum in rabid animals has not yet been studied. In this study, the nasal planum was obtained from 45 rabid dogs. In all rabid dogs, the viral antigen was detected in the peripheral nerve tissues, Merkel cells, and squamous cells. The viral antigen in the epidermis exhibited three patterns: first, a diffuse positive pattern from the basal layer to the squamous layer; second, a reticular positive pattern along the cell membrane in the squamous layer; and third, a basal layer pattern of the epidermis. In the dermis, viral antigens were detected more often in lamellated corpuscles just beneath the rete pegs. These results suggest that the nasal planum could serve as a useful alternative source for postmortem diagnosis in rabies endemic countries.

著者

井上 敏 義永 忠一 野尻 亘 Satoshi Inoue Tadakazu Yoshinaga Wataru Nojiri

出版者

桃山学院大学総合研究所

雑誌

桃山学院大学総合研究所紀要 = St. Andrew's University bulletin of the Research Institute (ISSN:1346048X)

巻号頁・発行日

vol.30, no.1, pp.21-42, 2004-07

---

Sensyu District is in the southern part of Osaka Prefecture. In this region, since the 18th century, many farmers had cultivated cotton and spun cotton into thread. However, because of the importation of cotton to Japan in the latter half of the 19th century, farmers had abandoned cultivation of cotton. On the other hand, the tradition of spinning cotton as a side job for farmers led the development of the thriving textile industries in this region. Up to World War II cotton textiles had been the most important export for Japan since the 19th century. Osaka City was the center of exporting cotton textiles. The location adjacent to Osaka City was advantageous for textile industries. Miscellaneous textile products, for example, cotton textiles, blankets, towels, knitwear, carpets etc. are manufactured by medium and small sized factories in Sensyu District. The networks of these companies and related industries have formed the agglomeration of textile industries in this region. The indigenous labor market is composed of specialized workers for textile industries. The trade unions and public institutions are engaged in quality control and development of new products for textile industries. Today, the incursion of cheap imports of textiles from Asia, the lack of successors in the workforce and the stagnation of productivity have gradually brought about the decline of textile industries in Sensyu District.

著者

Nozomi SHIWA Hiroko YAMASHITA Koki TOMIOKA Kazunori KIMITSUKI Daria Llenaresas MANALO Satoshi INOUE Chun-Ho PARK

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0591, (Released:2018-12-07)

被引用文献数

3

---

In the present study, follicle-sinus complexes (FSCs) were harvested from the muzzle skin of 123 dogs with suspected canine rabies, and the sensitivity and specificity of FSC analysis were compared with those of brain tissue immunohistochemistry analysis. In the FSCs, viral antigen was detected from Merkel cells. Sensitivity was 97.3%, specificity was 100%, and the coefficient κ was 0.88. These results reconfirm that FSCs are very useful for the postmortem diagnosis of canine rabies, and suggest that 5 FSCs can yield results that are almost equivalent to those derived from brain tissue analysis in rabid dogs.

著者

Nozomi SHIWA Chikage NAKAJIMA Kazunori KIMITSUKI Daria Llenaresas MANALO Akira NOGUCHI Satoshi INOUE Chun-Ho PARK

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0519, (Released:2018-10-17)

被引用文献数

3

---

Recently, we reported that follicle-sinus complexes (FSCs) in the muzzle skin are useful for postmortem diagnosis of rabid dogs. Here, we compared the sensitivity and specificity of detecting the viral antigen in the brain and FSCs of 226 suspected rabid dogs, and assessed whether the FSC harbored the virus genome and particles. The viral antigen was detected in 211 of 226 samples with 100% sensitivity and specificity. Viral RNA and particles were observed in the cytoplasm of Merkel cells (MCs). These results suggest that MCs are targets of virus infection and FSCs are useful material for diagnosing rabies.

著者

Tatsuya Hirano Tomohiko Kinoshita Daichi Kazamori Satoshi Inoue Kouji Nishimura Asuka Sakurai Kensuke Ohishi Yasuhiro Kuramoto Hirotaka Amano Akira Yazaki

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.66, no.3, pp.235-238, 2018-03-01 (Released:2018-03-01)

参考文献数

25

被引用文献数

5

---

WFQ-101 with a unique N-1 substituent, 5-amino-4-fluoro-2-(hydroxymethyl)phenyl group, was selected as a lead compound through combination screening based on antimicrobial activity and the efflux index against quinolone-resistant (QR) Pseudomonas aeruginosa (P. aeruginosa). Through structural optimization, we identified WFQ-228 as a novel fluoroquinolone antibiotic candidate. WFQ-228 had potent and superior activity in comparison to levofloxacin (LVX) and ciprofloxacin (CIP) against clinical isolates of P. aeruginosa, Escherichia coli and Acinetobacter baumannii, including QR strains. Furthermore, WFQ-228 demonstrated the potential to overcome major mechanisms of drug resistance; its antimicrobial activity was less affected by both pump-mediated efflux and mutations of the quinolone resistance-determining region in P. aeruginosa compared with LVX and CIP. These results suggest that WFQ-228 is a promising candidate for further evaluation in the treatment of infections caused by QR Gram-negative pathogens.

著者

Tomohiko Urano Satoshi Inoue

出版者

(社)日本内分泌学会

雑誌

Endocrine Journal (ISSN:09188959)

巻号頁・発行日

pp.EJ15-0154, (Released:2015-04-11)

被引用文献数

4 49

---

Osteoporosis is a skeletal disorder characterized by low bone mineral density (BMD) and an increased susceptibility to fractures. Evidence from genetic studies indicates that BMD, a complex quantitative trait with a normal distribution, is genetically controlled. Genome-wide association studies (GWAS) as well as studies using candidate gene approaches have identified single-nucleotide polymorphisms (SNPs) that are associated with BMD, osteoporosis and osteoporotic fractures. These SNPs have been mapped close to or within genes including those encoding WNT/β-catenin signaling proteins. Understanding the genetics of osteoporosis will help to identify novel candidates for diagnostic and therapeutic targets. Genetic factors are also important for the development of sarcopenia, which is characterized by a loss of lean body mass, and obesity, which is characterized by high fat mass. Hence, in this review, we discuss the genetic factors, identified by genetic studies, which regulate the body components related to osteoporosis, sarcopenia, and obesity.

著者

Chun-Ho PARK Daisuke KOJIMA Hitoshi HATAI Satoshi INOUE Toshifumi OYAMADA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.6, pp.787-790, 2012 (Released:2012-07-04)

参考文献数

15

被引用文献数

3 5

---

Traumatic neuroma of the cervical spinal cord was diagnosed in a 14-year-old male mixed-breed dog. A gross view showed two intradural extramedullary masses, measuring 1 and 0.6 cm in length and 0.7 and 0.4 cm in diameter, attached to the left side of the spinal cord at the level of the sixth and seventh cervical vertebrae. Microscopically, the cervical spinal masses comprised interlacing fascicles of axons and Schwann cells surrounded by collagenous stroma. Immunohistochemically, the fascicles were stained positively for neurofilament and S-100 proteins. Ultrastructurally, variably sized myelinated fibers and onion bulb-like structures were observed. To our knowledge, this is the first report of a traumatic neuroma in the cervical spinal cord of a dog.

著者

Chun-Ho PARK Daisuke KOJIMA Hitoshi HATAI Satoshi INOUE Toshifumi OYAMADA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.1112250744, (Released:2011-12-28)

被引用文献数

4 5

---

Traumatic neuroma of the cervical spinal cord was diagnosed in a 14-year-old male mixed-breed dog. A gross view showed two intradural extramedullary masses, measuring 1 cm and 0.6 cm in length and 0.7 cm and 0.4 cm in diameter, attached to the left side of the spinal cord at the level of the sixth and seventh cervical vertebrae. Microscopically, the cervical spinal masses comprised interlacing fascicles of axons and Schwann cells surrounded by collagenous stroma. Immunohistochemically, the fascicles stained positively for neurofilament and S-100 proteins. Ultrastructurally, variably sized myelinated fibers and onion bulb-like structures were observed. To our knowledge, this is the first report of a traumatic neuroma in the cervical spinal cord of a dog.