- 著者
- 松浦 滉明 坂井 直樹
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.63, no.3, pp.219-221, 2021-08-31 (Released:2021-09-02)
- 参考文献数
- 6
Automated data collection by the ZOO system has realized effective data collection for protein structure determination at SPring-8. Using the ZOO system, it is now available to collect diffraction data from hundreds of single crystals within a day. In order to analyze crystal structures using the huge amount of data, we have been developing an automated structure analysis pipeline. Furthermore, we have applied the highly efficient data collection systems of SPring-8 to high-throughput ligand screening using protein crystals.Here we report our recent developments on the automated structure analysis pipeline NABE system and the ligand screening pipeline using an acoustic liquid handler ECHO.
4 0 0 0 OA 点群,空間群の部分群
4 0 0 0 OA 電子線三次元結晶構造解析/3D ED/マイクロED
- 著者
- 米倉 功治
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.63, no.3, pp.189-196, 2021-08-31 (Released:2021-09-02)
- 参考文献数
- 49
The scattering powers of electron and X-ray differ by 4 - 5 orders of magnitude. Thanks to this property, the electron beam yields high-resolution diffraction spots from undersized crystals of various samples, which are hard to grow to a suitable size for X-ray diffraction even with a high-intensity synchrotron radiation beam. Thus, the technique known as electron 3D crystallography/3D ED/MicroED is recognized as being important especially in synthetic chemistry, material sciences and related areas, while single particle analysis can be used for larger-sized proteins. Here I review this technology including our recent developments and results.
4 0 0 0 OA 球・楕円・楕円体での最密充填
- 著者
- 松本 〓生
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.39, no.3, pp.254-257, 1997-06-28 (Released:2010-09-30)
- 参考文献数
- 20
4 0 0 0 OA リチウムに富む含水準輝石族新鉱物「村上石」の結晶化学
4 0 0 0 OA JCPDS-国際回折データ・センターについて
3 0 0 0 OA 植物が乾燥ストレスに応答する仕組み:アブシジン酸シグナル伝達の制御機構
- 著者
- 宮川 拓也 田之倉 優
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.53, no.3, pp.178-185, 2011-06-30 (Released:2011-07-13)
- 参考文献数
- 32
The phytohormone abscisic acid (ABA) plays a key role in the rapid adaptation of plants to environmental stresses such as drought and high salinity. Accumulated ABA in plant cells promotes stomatal closure in guard cells and transcription of stress-tolerant genes. Our understanding of ABA responses dramatically improved by the discovery of both PYR/PYL/RCAR as a soluble ABA receptor and inhibitory complex of a protein phospatase PP2C and a protein kinase SnRK2. Moreover, several structural analyses of PYR/PYL/RCAR revealed the mechanistic basis for the regulatory mechanism of ABA signaling, which provides a rational framework for the design of alternative agonists in future.
3 0 0 0 OA 結晶の相転移 (序論)
- 著者
- 松原 武生
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.14, no.6, pp.201-215, 1972-12-25 (Released:2010-09-30)
- 参考文献数
- 22
3 0 0 0 OA クライオ電子顕微鏡単粒子解析の実際~試料調製から画像解析まで~
- 著者
- 橋本 翼 横山 武司 田中 良和
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.63, no.2, pp.89-96, 2021-05-31 (Released:2021-06-05)
- 参考文献数
- 30
- 被引用文献数
- 1
Recent marked development called “Resolution revolution” has made cryo-electron microscopy (Cryo-EM) the third method of structure determination at atomic resolution next to X-ray crystallography and NMR. In this review, actual situation surrounding Cryo-EM including an outline about the workflow from sample preparation to image analysis and differences between Cryo-EM analysis and X-ray crystallography is introduced. We hope that this review is useful for researchers particularly who will start Cryo-EM analysis.
3 0 0 0 OA フラグメント分子軌道計算による構造解析
- 著者
- 福澤 薫 渡邉 千鶴 加藤 幸一郎
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.65, no.1, pp.17-25, 2023-02-28 (Released:2023-03-08)
- 参考文献数
- 19
The fragment molecular orbital(FMO)method is a theoretical method that enables quantum chemical calculations of whole bio-macromolecules, such as proteins and nucleic acids, yielding the energies and electron densities of whole molecules as well as interaction energies between fragments. The FMO calculations of protein complexes that have been structurally analyzed experimentally allow for quantitative analysis and physicochemical interpretation of intra- and intermolecular interactions within the complexes. Here we describe the quantitative analysis of structures and interactions using the FMO method, i.e., the “structure and interaction basis” obtained through the complementary use of experimental structure and the FMO calculations.
3 0 0 0 OA 空間群のヒエラルキーと分類
3 0 0 0 OA カラメルに含まれるオリゴ糖を分解する酵素の構造生物学的な洞察
3 0 0 0 OA 全自動測定による高難度結晶からの構造解析
- 著者
- 沼本 修孝
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.63, no.3, pp.229-231, 2021-08-31 (Released:2021-09-02)
- 参考文献数
- 5
Recent advance in the automatic data collection systems at the synchrotron facilities enable us to obtain high quality diffraction data without on-site operation. Structural determinations from the crystal clusters of small proteins were successfully achieved with microfocus beam, the automated data-collection system, and the data-processing pipeline in SPring-8.
3 0 0 0 OA 高分解能中性子構造から明らかになったタンパク質中のペプチド結合における新しい描像
3 0 0 0 OA ヘルマン・モーガン記号の解読
3 0 0 0 OA 収束電子回折法による結晶構造解析の進展
- 著者
- 森川 大輔 津田 健治
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.63, no.2, pp.135-142, 2021-05-31 (Released:2021-06-05)
- 参考文献数
- 36
Convergent-beam electron diffraction (CBED) is one of the powerful tools for nano-meter scale structural analysis. From its specific futures such as nano-meter probe, dynamical scattering, and direct determination of electrostatic potential, CBED can be applied for space group determination, observation of orbital-ordered state, and local structure analysis. In this paper, some examples for recent results obtained by CBED method are described.
3 0 0 0 OA 結晶の外形から何がわかるか
- 著者
- 長瀬 敏郎
- 出版者
- The Crystallographic Society of Japan
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.42, no.6, pp.469-473, 2000-12-22 (Released:2010-09-30)
- 参考文献数
- 25
- 被引用文献数
- 1 1
External form of polyhedral crystal provides information on the structural feature of the crystal. This article describes the relation between the external form and the structure of crystals.
3 0 0 0 OA クロマチンのクライオ電子顕微鏡構造解析
- 著者
- 滝沢 由政 胡桃坂 仁志
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.64, no.1, pp.65-68, 2022-02-28 (Released:2022-03-04)
- 参考文献数
- 9
Chromatin is a highly organized complex of genomic DNA and proteins in eukaryotic cells. Fundamental unit of chromatin is nucleosome composed of histone octamer and approximately 150 base pairs of DNA. The higher-order conformation of chromatin is involved in important genomic events such as replication, transcription, recombination, and repair. Recently various structures chromatin have been determined by cryo-electron microscopy (cryo-EM). Based on our cryo-EM studies, in this article, we introduce current structures of chromatin determined by cryo-EM.
3 0 0 0 OA タンパク質結晶構造解析入門
- 著者
- 芦田 玉一
- 出版者
- The Crystallographic Society of Japan
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.38, no.6, pp.378-388, 1996-12-30 (Released:2010-09-30)
- 参考文献数
- 4
Introduction to elementary protein crystallography including items such as, features of protein crystals, crystallization of proteins, the heavy atom isomorphous replacement method, model building and refinement, and a brief history of proten crystallography, is presented.
3 0 0 0 OA 氷の相転移
- 著者
- 河田 脩二
- 出版者
- The Crystallographic Society of Japan
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.40, no.1, pp.32-36, 1998-02-28 (Released:2010-09-30)
- 参考文献数
- 38
Phase transitions in ice are summarized in aspect of the hydrogen bond and its network Recent works and developments in phase transition from Ih to the ordered phase, phase transitions between high pressure phases: VII, VIII and X, and amorphous-amorphous phase transition are centrally reviewed.